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A special multifunctional growth-promoting bacterium agent for soybean and its preparation method and application

A growth-promoting bacterium agent and multi-functional technology, applied in the field of microorganisms, can solve the problems that it is difficult to meet the requirements of various nutrient elements of plants, and cannot guarantee the long-term survival of the bacterium agent, so as to increase soybean yield, increase potassium content, and increase quick-acting Effect of Potassium Content

Active Publication Date: 2020-11-24
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ordinary microbial fertilizers such as phosphorus-dissolving bacteria or potassium-dissolving bacteria can only provide plants with a single nutrient element, which is difficult to meet the requirements of plants for multiple nutrients, and cannot guarantee that the bacteria agent can survive in the plant rhizosphere for a long time and play a stable role

Method used

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  • A special multifunctional growth-promoting bacterium agent for soybean and its preparation method and application
  • A special multifunctional growth-promoting bacterium agent for soybean and its preparation method and application
  • A special multifunctional growth-promoting bacterium agent for soybean and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0033] The screening of embodiment 1 bacterial strain

[0034] The strain screening method is as follows:

[0035] Obtain young soybean roots grown for 28-32 days, and put them into -1 Nystatin in sterile saline, at 28°C, 100-200r·min -1 Shake culture for 1-2h, adopt the ten-fold gradient method, and make 10 -1 to 10 -5 Concentration gradient bacterial suspension, respectively take 100-200μL from the bacterial suspension of each dilution and spread it on the phosphate-solubilizing bacteria solid medium plate with a pH of 7.0-7.2. K 2 HPO 4 2.0g, ammonium sulfate 0.5g, NaCl 0.3g, KCl 0.3g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, Ca 3 (PO 4 ) 2 10g, 15g agar, 1000mL distilled water. Cultivate at 27-29°C for 24-36 hours, pick colonies with phosphorus-dissolving circles, separate and purify to obtain a single colony of phosphorus-dissolving bacteria; the obtained phosphorus-dissolving bacteria strains are inoculated into potassium-dissolvin...

Embodiment 2

[0037] Determination of Phosphorus and Potassium Ability of Example 2 Bacterial Strains

[0038] A total of 20 growth-promoting bacterial strains with dual functions of dissolving phosphorus and potassium, which have specific affinity with soybean, were screened from the soybean rhizosphere by selective medium and lectin double screening method, and the phosphorus-dissolving and potassium-dissolving ability of these strains were determined. ability.

[0039] Prepare the bacterial suspension of each strain, insert the bacterial strain into the phosphate-dissolving medium containing tricalcium phosphate, calcium phytate and lecithin with the inoculum size of 2%, and the medium formula is glucose 10g, K 2 HPO 4 2.0g, ammonium sulfate 0.5g, NaCl 0.3g, KCl 0.3g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, insoluble phosphorus 10g, distilled water 1000mL, pH 7.2, and at 28℃, 160r·min -1 Shaking culture 5d. The content of available phosphorus in the cult...

Embodiment 3

[0044] The identification of embodiment 3 bacterial strains

[0045] Combining morphological observation, physiological and biochemical tests and 16SrRNA gene sequence comparison analysis to identify the species of FK19 strain.

[0046] According to "Common Bacteria System Identification Manual" and "Bergey's Bacterial Identification Manual", the physiological and biochemical characteristics of FK19 strain were detected. The morphological tests include Gram staining; the physiological and biochemical tests include starch hydrolysis test, V-P test, contact enzyme test, glucose oxidative decomposition test, hydrogen sulfide test, gelatin liquefaction test, and methyl red test. The results are shown in Table 2.

[0047] Table 2 Some physiological and biochemical characteristics of the strains

[0048]

[0049] The 16S rRNA gene sequence of the FK19 strain was determined, similar sequences were searched in the NCBI database, compared, and combined with the structure of physiol...

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Abstract

The invention belongs to the technical field of microorganisms, and in particular relates to a special multifunctional growth-promoting bacterium agent for soybeans, which is fermented from the FK19 strain, and the classification of the FK19 strain is named Bacillus megaterium (Bacillus megaterium), preserved on December 27, 2018 In China Center for Type Culture Collection, the deposit number is CCTCC M 2018925. The beneficial effects of the present invention are: the multifunctional growth-promoting bacterium agent of the present invention has obvious promoting effect on soybean growth, has obvious increasing effect on phosphorus content and potassium content of soybean leaves, and can increase soybean yield. Soybean roots will produce soybean lectin, which is a sugar-binding protein or glycoprotein of non-immune origin, which can be combined with polysaccharides of bacteria. The multifunctional growth-promoting bacteria agent of the present invention has soybean lectin affinity, and can Combined, adsorbed on the soybean roots, and then promote the synthesis of more isoflavones in soybeans, mediate the colonization of the bacterial strains in the soybean roots, so as to exert the growth-promoting function of the bacterial agent.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a special multifunctional growth-promoting bacterium agent for soybeans, a preparation method and application thereof. Background technique [0002] Soybean is one of the main economic crops in my country and has a wide planting rate in China. Phosphorus and potassium are of great significance to soybean material accumulation, yield and quality. my country has a large population and little cultivated land. Although the soil is rich in phosphorus and potassium, most of them exist in the form of insoluble phosphorus and potassium, which are difficult to be absorbed and utilized by soybeans. In order to improve the yield and quality of soybeans, a large amount of phosphorus and potassium fertilizers are often used in agriculture, but the utilization rate of soybeans for phosphorus and potassium fertilizers is too low, and they cannot absorb the nutrients given by...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/11
CPCC12N1/20C12N1/205C12R2001/11
Inventor 曹媛媛郭婷婷李婷黎权德何相怡邱丽娟王晓波陈春钱叶张程飞
Owner ANHUI AGRICULTURAL UNIVERSITY
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