Fermentation culture of Bacillus vallismortis and production method of compound microbial fertilizer

A technology of Bacillus dead valley and complex microorganisms, which is applied in the field of microbial fermentation and cultivation, can solve the problems of farmland soil deterioration, quality reduction, and fertility decline, and achieve the effects of increasing soil moisture content, reducing disposal costs, and comprehensive nutrients

Active Publication Date: 2014-07-09
SHANGHAI LVLE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, agricultural production has been overly dependent on chemical fertilizers for a long time, resulting in the waste of a large amount of non-renewable energy, the deterioration of farmland soil quality, the decline of fertility, the residues and quality reduction of crop products, and the problems of food and environmental pollution have become increasingly prominent.
Therefore, relying solely on the combination of water-retaining agents and inorganic fertilizers to produce water-retaining slow-release fertilizers cannot meet the requirements of sustainable and green agricultural development.

Method used

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  • Fermentation culture of Bacillus vallismortis and production method of compound microbial fertilizer
  • Fermentation culture of Bacillus vallismortis and production method of compound microbial fertilizer
  • Fermentation culture of Bacillus vallismortis and production method of compound microbial fertilizer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Strain fermentation: the basic medium is sodium glutamate 10g / L, MgSO 4 ·7H 2 O 0.25g / L, CaCl 2 2H 2 O 0.1g / L, K 2 HPO 4 When 2g / L, the fermentation conditions of the Bacillus dead valley strain are: temperature 32°C, liquid volume 20%, medium initial pH 6.5, yeast extract as nitrogen source, dosage 5g / L, saccharified corn flour as carbon source, fermented at a dosage of 15g / L, and the amount of bacteria obtained after 48 hours of fermentation was 3.9×10 10 CFU / mL;

[0041] 2. Sampling of amino acid waste: the hydrolyzed slag was obtained from Henan Lianhua Amino Acid Co., Ltd.;

[0042] 3. pH adjustment: Add 8% potassium hydroxide to the hydrolyzed slag, mix it, crush it to 80 mesh with a chain crusher, and stack it for 8-12 hours to allow it to fully react. The basic properties of hydrolyzed slag after treatment are shown in Table 1;

[0043] Table 1 Basic properties of hydrolyzed slag (powder) after treatment

[0044]

[0045] 4. Turn over: Use a fork...

Embodiment 2

[0057] The fermentation conditions of the strain are as follows: temperature 30°C, liquid volume 25%, medium initial pH 7.0, yeast extract as the nitrogen source, the dosage is 10g / L, saccharified corn flour is the carbon source, and the dosage is 20g / L. After 48 hours of fermentation, the amount of bacteria that can be obtained is 4.5×10 10 CFU / mL;

[0058] Add 6% potassium hydroxide to the hydrolyzed slag, mix it, crush it to 100 mesh with a chain crusher, and stack it for 15 hours to allow it to fully react;

[0059] In the preparation of microbial inoculum, the fineness of rice husk powder is 100 mesh, and the adsorption mass ratio of inoculum to rice husk powder is 1:5. The three adsorbed single bacterial agents were mixed according to the mass ratio of Bacillus dead valley: Bacillus subtilis: Bacillus jelly-like = 10:3:7.

[0060] The addition ratio of the bacterial agent is 6%, and the addition ratio of the hydrolyzed slag is 64%, both of which are the mass percentage...

Embodiment 3

[0062] Add 6% potassium hydroxide to the hydrolyzed slag, mix it with a chain crusher to 120 mesh, and stack it for 8-12 hours to allow it to fully react;

[0063] In the preparation of water-retaining composite microbial agent, the fineness of rice husk powder is 120 mesh, and the adsorption ratio of bacterial liquid to rice husk powder is 1:4. The three kinds of single bacterial agents adsorbed respectively are mixed according to the mass ratio of Bacillus dead valley: Bacillus subtilis: Bacillus jelly-like = 15:10:7, and then pulverized to 80 mesh.

[0064] The addition ratio of the bacteria agent is 8%, the addition ratio of the hydrolyzed slag is 63%, and the addition ratio of urea is 14%, all of which are the mass percentages of the final finished fertilizer. Others are with embodiment 1.

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Abstract

The invention provides a fermentation culture method of Bacillus vallismortis, which comprises a step of seed strain culture and a step of fermentation culture. In the fermentation culture step, the initial pH of the culture medium is 6.0-7.0, the strain temperature is 30-37 DEG C, the nitrogen source consumption is 5-10 g / L, and the carbon source consumption is 15-25 g / L. The invention also provides a method for producing a compound microbial fertilizer from hydrolysis residues of the Bacillus vallismortis fermentation liquid. The three strains of Bacillus vallismortis, Bacillus subtilis and Bacillus mucilaginosus are cultured separately and then mixed to prepare a compound microbial agent, and the compound microbial agent is further processed to a compound microbial fertilizer. The count of viable microorganisms or spores in the fermentation liquid (48 hours) produced by the fermentation culture method is more than (3.4-5.1)*10<10> CFU / mL, which is at least one magnitude order higher than the prior art. Besides, the production method of the microbial fertilizer effectively utilizes waste resources and is advantageous in environmental friendliness and economic efficiency.

Description

technical field [0001] The invention relates to a method for fermenting and cultivating microorganisms, in particular to a method for fermenting and cultivating bacillus and a method for preparing microbial fertilizer from the fermented liquid. Background technique [0002] In recent years, extreme weather conditions have occurred frequently, especially dry climates, and almost all parts of the country have not been spared. For more than three consecutive months in 2009, 15 provinces and cities in North China, Huanghuai, Northwest, Jianghuai and other places did not see effective precipitation. Affected by the severe drought, the water level of the Yangtze River dropped, causing Shanghai to experience rare and severe salty tides in May that would normally occur in winter, causing some water supply areas to become urgent. The impact of the drought on agricultural production is obvious. Although the country has introduced various effective measures to stabilize the excessive ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/07
Inventor 闫龙翔陈芳陈露
Owner SHANGHAI LVLE BIO TECH
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