299 results about "Geonoma congesta" patented technology

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

The invention discloses a composite microbial inoculum organic multielement compound fertilizer which is prepared from microbial inoculum, organic component and inorganic fertilizer. The microbial inoculum is a composite microbial inoculum which comprises the following three types of microbial inocula: resource type microbial inoculum, which contains Bacillus megaterium, thiobacillus thiooxidans, Bacillus mucilaginosus and Bacillus circulans; environment-friendly microbial inoculum, which contains Arthrobacter and Alcaligenes; and antibiotic microbial inoculum, which contains Streptomycs micuoflavus and Streptomyces jingyangensis. The organic component is one or more of cereal crop straw, subsidiary agricultural product dreg, sugar mill bagasse, bran coat, municipal and rural domestic waste, and livestock and poultry dung. The inorganic fertilizer is one or more of nitrogen fertilizer, phosphate fertilizer, potash fertilizer and medium/micro-nutrient element fertilizer. The weight proportion of microbial inoculum to organic constituent to inorganic fertilizer is (5-25):(18-28):(47-77).

The invention relates to a liquid compound biological fertilizer with long shelf life as well as a preparation method and application thereof. The liquid compound biological bacterial fertilizer comprises the following components in parts by weight: 2-5 parts of microbial thalli, 80-130 parts of compound nutrients, 10-30 parts of thalli protective agents and 0.5-3.0 parts of additives, wherein the microbial thalli is metered in terms of the wet weight of deposition obtained after fermentation liquor is centrifugalized, the microbial thalli include bacillus thuringiensis, bacillus megaterium, bacillus mucilaginosus and bacillus amyloliquefaciens, and the total viable count achieves 5*10<8>-5*10<9>/mL. The liquid compound biological bacterial fertilizer disclosed by the invention can be used as a biological pesticide, can be directly applied to the spraying of plant surfaces or applied to seed soaking, root irrigation, and the like, has the effects of insect resistance, phosphate solubilization, potassium solubilization, organic matter solubilization, pest resistance, and the like and is long in shelf life.

The invention discloses a biological fertilizer, comprising, by weight, 65-80 parts of a concentrated nmolasses fermentation broth, 7-14 parts of potassium humate, 0.5-2 parts of a chelated microelement, 1-5 parts of microbial strains and 5-10 parts of a surfactant, wherein the chelated microelement is a mixture of EDTA-Cu, EDTA-Fe, EDTA-Mn and EDTA-Zn; and the microbial strains are composed of paenibacillus polymyxa, bacillus subtilis and bacillus mucilaginosus. The biological fertilizer is an environment-friendly type fertilizer, can improve soil environment and increase crop output, can be widely applied in plants particularly crops, has a very significant yield-increasing effect, and can increase the output of food crops, vegetables, melons and fruits by 10%-20%.

The invention is suitable for the technical field of recycling organic wastes, and discloses a flora for recycling treatment of organic wastes. The flora for recycling treatment of organic wastes is efficiently compounded from a variety of bacteria such as bacillus subtilis, aspergillus niger, saccharomycete and soil flora, wherein the soil flora is extracted from the natural world and comprises three or more of bacillus megatherium, bacillus mucilaginosus, bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis, mycorrhiza fungi, azotobacter vinelandii, photosynthetic bacteria, bacillus coagulans, aspergillus oryzae, and paecilomyces lilacinus. According to the flora for recycling treatment of organic wastes, disclosed by the invention, by virtue of mutual promotion, mutual coordination and combined action of a variety of beneficial microorganisms, the organic wastes are recycled, environmental pollution is reduced, and wastes are changed into valuables; and the flora for recycling treatment of organic wastes can be used for producing industrial fermentation products and improving the health and growth conditions of animals, and can also improve the current situation of serious soil hardening at present and improve soil quality, thereby promoting the rapid development of agricultural economics.

The invention relates to the field of microbiology, and specifically relates to a composite microbial fertilizer and a preparation method thereof. The composite microbial fertilizer is composed of a composite microbial fermentation broth and an embedding medium, wherein a volume ratio of the composite microbial fermentation broth to the embedding medium are in a range of 1:5 to 1:4. The compositemicrobial fermentation broth is compounded of a co-culture comprising Candida utilis, lactic streptococci, lactobacillus acidophilus and bacillus subtilis, and a single nutrient solution comprising Bradyrhizobium japonicum, Azospirillum lipoferum, Rhodopseudomonas palustris, Bacillus megaterium, Bacillus mucilaginosus and Azotobacter chrococcum. In the invention, composite microbes processed by embedding can be stored stably for more than 60 days and a count of the composite microbes is always more than 2 billion per gram, thus the composite microbial fertilizer comprising the composite microbes has a long effective time and can improve a planting capacity of soil.

The invention discloses a method for treating culturing wastewater through utilization of a microorganism flocculant. The method comprises the following steps: 1) selection of bacillus mucilaginosus as a producing strain; 2) activation, fermentation and cultivation of a strain, wherein the strain is activated by a selective culture medium, inoculated in a culture medium for producing a seed strain and cultured to prepare the seed strain; and the seed strain is inoculated in a liquid fermentation culture medium and is fermented to obtain a fermentation liquid; 3) preparation of the microorganism flocculant, wherein the fermentation liquid is naturally precipitated; and a supernatant fluid is removed to prepare the microorganism flocculant; and 4) treatment on the culturing wastewater, wherein the microorganism flocculant and the culturing wastewater in volume ratio of 1 : 40-60 are mixed for 30 to 45 minutes and filtered; and filter residue is removed. The invention also provides a composite fertilizer prepared according to the method. The culturing wastewater treated by the method can reach emission standard and can also prepare composite fertilizer with good fertilizing effect.

The present invention discloses one kind of fast harmless biological treating process of animal excrement and manure water, and has six kinds of functional microbes combined for fast and efficient treatment. Six kinds of functional microbes, including Bacillus cereus, glial bacillus, trichoderma, curdy bacillus, saccharomycete and Bacillus subtilis are combined to form compound microbial preparation; and the compound microbial preparation is further combined with humic acid and inoculated to animal excrement and manure water in pig farm. Through adding filler, fermentation and other steps, animal excrement and manure water are converted into organic fertilizer. The present invention can utilize the resource of animal excrement and manure water effectively.

The invention discloses a biological control agent for fusarium wilt and a preparation and use method of the biological control agent. The biological control agent comprises the following components of not less than 1*10<7>cfu/mL of cellulose-producing trichoderma viride, not less than 0.5*10<7>cfu/mL of aspergillus niger, 0.8*10<9>cfu/mL of bacillus subtilis and 0.8*10<9>cfu/mL of bacillus mucilaginosus. The prepared biological control agent has an antagonism on antagonism, is capable of increasing disease prevention capability, and has functions of activating soil nutrients, promoting growth of root systems of crops, and inducing the improvement of disease resistance when being applied, thereby improving control effect of fusarium wilt of the crops, and enabling the incidence rate to be lower than 10 percent. The biological control agent can completely replace a chemical agent, has no pollution, residue and biological toxicity during use, ensures that the yield of crops is increased by above 15 percent, and is suitable for controlling fusarium wilt of cucurbit plants such as cucumber, watermelon, muskmelon, bottle gourd and towel gourd, and solanaceae crops such as tomato and eggplant.

The invention belongs to the technical field of saline-alkali soil improvement and particularly relates to a saline-alkaline soil improving agent. The saline-alkali soil improving agent is characterized by comprising the following raw materials in parts by weight: 10-15 parts of flue gas desulfurization wastes, 20-30 parts of mixed grass meal, 3-8 parts of compound microorganisms, 5-15 parts of sawdust, 6-15 parts of sweet clover root powder and 10-15 parts of kieselguhr, wherein the mixed grass meal consists of sainfoin, symphytum officinale, coronilla varia and festuca arundinacea, and the compound microorganisms consist of nitrogen-fixing bacteria, Bacillus subtilis, bacillus licheniformis and bacillus mucilaginosus. The improving agent disclosed by the invention not only can greatly and effectively improve the current situations of saline-alkali soil, but also can utilize wastes and recycle resources and ecological vegetation, and is environment-friendly, safe and free of secondary pollution problem.

The invention provides a novel compound microbial fertilizer containing Pulullan and polyglutamic acid and a preparation method of the novel compound microbial fertilizer. The compound microbial fertilizer comprises a microbial fermentation inoculum, compound nutrients, Pulullan and polyglutamic acid, wherein the microbial fermentation inoculum is prepared by mixing fermentation liquors of bacillus megatherium, bacillus amyloliquefaciens, bacillus thuringiensis, bacillus mucilaginosus and azotobacter chroococcum according to the mass ratio of 1: 1: 1: 1: 1; the compound nutrients comprise nitrogen, phosphorus, potassium, trace elements, organic matters and compound amino acids. By applying the microbial fertilizer, the bud ratio, the plant height and the yield of highland barley can be obviously improved, and the highland barley is excellent in taste. Furthermore, the use level of irrigation water can be reduced by 10% due to the film-forming property of Pulullan and the water-retaining property of polyglutamic acid. In addition, Pulullan and polyglutamic acid are mixed to form a liquid mulch on the surface of soil, so that the use of a plastic thin film can be reduced, an environmental protective role can be played, the labor force is alleviated, and the cost is lowered by 10-20%.

The invention belongs to the field of the manufacturing of organic fertilizers and discloses a method for producing an amino acid microbial organic fertilizer by using cassava residue and starch waste liquor. The method comprises the following steps of: dehydrating the cassava residue and the starch waste liquor until the final water content is 60 to 65 weight percent; adding 8 to 12 weight percent of humic acid, 3 to 7 weight percent of oil cake, 3 to 7 weight percent of rice bran and 0.3 to 0.5 weight percent of straw fermentation decomposing inoculant, and performing aerobic fermentation, wherein the fermentation temperature is 40 to 65 DEG C, and the fermentation cycle is 15 to 20 days; and uniformly mixing a functional strain which accounts for 3 to 5 percent of the total weight and is one or more of Bacillus mucilaginosus, bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Streptomyces jingyangensis, starch-containing waste liquor which accounts for 2 percent of the total weight and fermented residue, aerating the air and performing late curing for 5 to 7 days, and adding 1 to 3 percent of amino acid to obtain a finished product. Raw materials do not contain harmful bacteria and pathogens, harmful gas and odor are not generated in the fermentation, and the fertilizer can increase effect and stimulate plants to grow quickly.

The invention discloses a sewage treating agent for heavy metal pollution. The sewage treating agent is prepared from raw materials in parts by weight as follows: polyethyleneimine, carbon disulfide,sodium hydroxide, starch xanthate, bacillus mucilaginosus, concha haliotidis powder, modified zeolite powder, modified bentonite, sodium alginate, humic acid, ferric trichloride, nanometer titania, cellulose, ferrous sulfate, a photocatalyst, an efficient nickel remover, a chitosan modified polymeric flocculant, a coagulant aid, sephadex and deionized water. A preparation method of the sewage treating agent comprises steps as follows: material preparation, preparation of a mixed liquor A, preparation of a mixed liquor B and forming of the sewage treating agent. Through addition of polyethyleneimine, carbon disulfide and the coagulant aid, the sewage treating agent has a good flocculation effect on various heavy metal ions; through addition of starch xanthic acid, bacillus mucilaginosus, the concha haliotidis powder, the modified zeolite powder and the modified bentonite, the sewage treating agent has a good adsorbing effect, and the heavy metal ions in sewage are effectively removed bydouble cooperation of adsorption and flocculation.

The invention discloses a complex bacterium preparation for fixing nitrogen, solubilizing phosphorus and potassium and rooting and a preparation method thereof. The preparation is mainly prepared by mixing the following raw materials in parts by weight: 5-20 parts of azotobacter vinelandii, 10-30 parts of bacillus megatherium, 10-30 parts of bacillus mucilaginosus, 5-15 parts of azotobacter chroococcum, 10-15 parts of glomus intraradices and 10-25 parts of glomus mosseae. The preparation method comprises the following steps: respectively carrying out high-density culture on each strain; grafting to a shake flask filled with a culture medium in a volume ratio of 15-30 percent for activated culture; grafting the cultured strains into a fermentation tank for fermentation culture expansion; dehydrating and drying the culture expanded single strains to prepare hypopus microorganism dried powder; and mixing to obtain the complex bacterium preparation for fixing nitrogen, solubilizing phosphorus and potassium and rooting. The complex bacterium preparation is used for industrial crops, fruit trees, food crops and the like, has the advantages of biological property, environmental friendliness, high yield and high quality in comparison with existing products, and cannot pollute soil.

The invention belongs to the field of wastewater treatment and discloses a treatment process applicable to industrial wastewater. The treatment process applicable to the industrial wastewater comprises the following steps: 1) preparing complex microbial inoculant; 2) precipitating wastewater; 3) purifying the wastewater; 4) preparing bacterial manure. The prepared complex microbial inoculant comprises 5-6 parts of micrococcus luteus, 4-5 parts of bacillus mucilaginosus, 3-4 parts of paracoccus denitrificans, 2-3 parts of rhodococcus, 2-3 parts of acidithiobacillus ferrooxidans, 1-2 parts of bacillus megaterium and 1-2 parts of bacillus cereus. The invention also discloses bacterial manure prepared by utilizing the treatment process applicable to the industrial wastewater. The treatment process applicable to the industrial wastewater is simple and feasible, the industrial wastewater can be effectively treated, and the bacterial manure is prepared by utilizing the treatment process applicable to the industrial wastewater.

The invention discloses a composite organic fertilizer prepared from vegetable wastes. The composite organic fertilizer is a fermented product which is obtained by fermenting a composite microbes and the vegetable wastes, wherein the composite microbe is a mixture of bacillus mucilaginosus, bacillus subtilis, tiny bacillus, bacillus megatherium, pseudomonas aeruginosa, plant lactic acid bacteria and azotobacter chroococcum, the number ratio of all the strains is 1:(7-8):(4-6):(3-5):(1-3):(3-5):(1-2), and the total amount of viable bacteria is 2.0*10<9>-3.0*10<9> CFU/mL. The invention also discloses a preparation method of the composite organic fertilizer. The preparation method comprises the following steps: inoculating the microbes to prepare a culture medium; and adsorbing by using an adsorbent, and fermenting the vegetable wastes to obtain compost. The compost prepared by the preparation method disclosed by the invention can be used for promoting the growth development of plants, and reducing plant diseases and insect pests and reducing the use amount of the inorganic fertilizer, so that the vegetable wastes are effectively used, and pollution of wastes to farm environments is reduced.

The invention discloses a bio-control composite bacterial agent which comprises, by weight, bacterial strains including 8-12 parts of purpureocillium lilacinum, 18-22 parts of pseudomonas fluorescens, 18-22 parts of bacillus cereus, 8-12 parts of bacillus mucilaginosus, 18-22 parts of bacillus subtilis, 8-12 parts of bacillus megaterium, 4-6 parts of bacillus thuringiensis and 4-6 parts of trichoderma viride and a culture medium comprising 0.8-1.2 parts of beef extract, 2.5-4 parts of potassium dihydrogen phosphate, 1.3-1.8 parts of urea, 10-12 parts of molasses, 1.5-2.5 parts of amino acid, 2.5-3.5 parts of glucose, 0.3-0.6 part of peptone, 0.25-0.4 part of sodium chloride and 0.35-0.6 part of potassium nitrate. The invention further comprises a preparation method of the bacterial agent. The bio-control composite bacterial agent has good effect on solving current problems of soil improvement, environment protection of agriculture and waste straw rubbish and can improve soil and accelerate crop growth and organic matter decomposition.

The invention relates to a technology for utilizing an agricultural microbial agent to prevent and treat crops polluted by soil heavy metal, belonging to the technical field of bioengineering. According to the technology, the agricultural microbial agent is adopted to prevent the roots of the crops to absorb soil heavy metal so as to achieve the purpose of preventing and treating pollution. According to the invention, the good functional bacteria, namely bacillus subtilis and bacillus mucilaginosus in the soil with overproof heavy metal, are adopted. The functional bacteria are utilized to absorb heavy metal ions in the growth process so as to conduct oxidation-reduction reaction, helpful substances are replaced to form methylation, and the generated organic metal is discharged. Due to the action of the floras added repeatedly, the micro molecules of the heavy metal are combined into macromolecules which can not be absorbed by the roots of the crops, so that the pollution is blocked.

The invention relates to a complex lively bacterial preparation which is prepared through the following method: (1) mixing the seed solutions of bacillus subtilis with an OD value of 3.5 to 4.0, saccharomycetes with an OD value of 3.0 to 4.0, bacillus mucilaginosus with an OD value of 3.5 to 4.0 and bacillus licheniformis with an OD value of 3.5 to 4.0 according to the volume ratio of (1 to 5):(1 to 5):(1 to 5):(1 to 5), so as to obtain a mixed seed solution;(2) carrying out high density mixed fermentation cultivation on the mixed seed solution in a by-product produced during yeast fermentation, and not stopping until the total of viable counts is larger than 1 billion/mL, so as to obtain the complex lively bacterial preparation. The complex lively bacterial preparation provided by the invention can be mixed with nutrient elements such as nitrogen and phosphorus to prepare a microorganism water soluble fertilizer, and the water soluble fertilizer prepared from the complex lively bacterial preparation is comprehensive in nutrition and can be easily absorbed by plants, the preparation raw materials of the fertilizer are cheap and easy to obtain, and the microorganism therein can inhibit the growth of pathogenic bacteria, promote the growth of crops and induce the plants to generate resistance, and takes the effects of prophylaxis and growth promoting on the crops.

The invention discloses a method used for preparing solid waste building material products based on microorganism mineralization induction technology. The method comprises following steps: a corresponding culture medium is inoculated with bacillus mucilaginosus for culturing, and a concentrated bacteria solution with a bacteria concentration ranging from 10<6> to 10<7>/ml is prepared; solid waste is mixed with an exciting agent, an obtained mixture is delivered into an agitator kettle for uniform sitting, the concentrated bacteria solution and water are added into the agitator kettle, after uniform stirring, sand is added, stirring is carried out continuously, and after uniform stirring an obtained slurry is delivered into a mould for vibration molding; after molding, obtained test pieces are subjected to curing at a relative humidity ranging from 57% to 63% at a temperature ranging from 18 to 22 DEG C; demolding is carried out in 1 day, and the test pieces are delivered into a pressure kettle for 4h of curing at a relative humidity ranging from 67% to 73% under CO2 pressure so as to obtain the solid waste building material products. The method is low in cost, and excellent in effect, and is friendly to the environment, and no secondary pollution is caused.

The invention discloses a preparation method of a bacterial fertilizer or a bacterial product. The preparation method is characterized by comprising the following steps: uniformly mixing a type A microbial agent and a type B microbial agent in the ratio of 1 to (0.1 to 10); adding the uniformly-mixed microbial agent into a pesticide residue culture medium to obtain the bacterial fertilizer or thebacterial product. Active components of the microbial agent consist of rhizobium japonicum, bacillus megaterium, bacillus cereus, bacillus subtilis, bacillus mucilaginosus, lactobacillus buchneri, bacillus amyloliquefaciens, paenibacillus polymyxa, bacillus pumillus, bacillus licheniformis, trichoderma harzianum, etc. The microbial agent and pesticide residues are mixed uniformly to obtain the bacterial fertilizer or the bacterial product. The bacterial fertilizer or the bacterial product can be applied to the facilitation of crop growth or the improvement of crop yield or the improvement of planting soil, as well as the improvement of crop disease resistance. The product can be combined with different bacterial fertilizer carriers to form a solid bacterial fertilizer, a dry or gel starchbacterial fertilizer, a liquid bacterial fertilizer, a pesticide residue waste bacterial fertilizer, a grass charcoal bacterial fertilizer, a peat bacterial fertilizer, a hibernated bacterial fertilizer, as well as a pig, cattle, sheep and chicken manure excrement bacterial fertilizer, and to form seed coatings.

The invention relates to fertilizer pesticide, and specifically relates to an organic fertilizer fermenting agent and a preparation method thereof. The fermenting agent is composed of bacillus subtilis, bacillus mucilaginosus, trichoderma viride, lactococcus lactis, actinomycetes, azotobacter chroococcum, bacillus megatherium, bacillus mucilaginosus, escherichia coli, active ingredient, cellulose, protease and phosphorylase. The preparation method of the fermenting agent comprises the following steps: 1, weighing each constituent; 2, preparing a mixture I; 3, preparing a mixture II; 4, smashing. According to the invention, active ingredient is added into the fermenting agent, so that effective viable count content in the fermenting agent is improved greatly, heating and fermenting time is reduced, and absorption and utilization rates of the fermenting agent by poultry dung, straw, and the like, are improved. Aspergillus niger in the active ingredient is uniformly stirred with other constituents after being stirred well with orangeade, thus, absorption and utilization of the organics in the active ingredient by various microbes in the fermenting agent are promoted greatly, and the increase of effective viable count in the fermenting agent is facilitated.

The invention relates to cellulase production bacillus licheniformis with phosphate-solubilizing and potassium-releasing functions and the application of the cellulase production bacillus licheniformis. The invention discloses the bacillus licheniformis BL-1 with the preserving number of CCTCC No. M 2012457 and the bacterial strain can produce cellulose, which has an excellent phosphate-solubilizing and potassium-releasing function and can be made into composite probiotics through scientific formulation to microzyme, bacillus subtilis, bacillus megatherium, bacillus mucilaginosus and azotbacteria chroococum, and microbial active sludge organic fertilizer added with the composite probiotics in proportion is directly applied to plant growth regions or applied to plant growth regions after compost fermentation, and has excellent soil enriching and yield increasing effects. According to the invention, efficient resource comprehensive utilization to active sludge discarded by fuel ethanol manufacturers can be achieved, meanwhile, environment pollution is avoided, the treatment process is simple, and the application cost is low. Therefore, ecological protection and sustainable development to agriculture can be facilitated.

The invention discloses a formula of a water body remediation microbial inoculant and a preparation method of the remediation microbial inoculant, and particularly relates to the field of sewage treatment. The water body remediation microbial inoculant comprises a microbial agent and a carrier, wherein the microbial agent comprises nitrobacteria, denitrifying bacteria, fibrotic nocardia, lactobacillus plantarum, candida utilis, bacillus subtilis and bacillus mucilaginosus, and the carrier comprises wheat bran, zein and zeolite powder. Though adding the complex inoculantwhich is extremely easy in survival and reproduction into the water body remediation microbial inoculant, a beneficial dominant community is formed, and the ecological structure of microorganisms in a water body is regulated and controlled; the microbial inoculant can degrade and transform the harmful substances in polluted water, promote the growth of indigenous flora, and play a role in purifying water quality, more importantly, various microbial flora contained in the microbial inoculant can promote the growth of aquatic plants, prevent and control aquatic animal diseases, and inhibit excessive reproduction of algae, and finally the effect of stabilizing and restoring the ecological environment of the water body is achieved.