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Cellulase production bacillus licheniformis with phosphate-solubilizing and potassium-releasing function and application of same

A technology of bacillus licheniformis and cellulase, applied in the field of microbial technology and microbial fertilizer

Active Publication Date: 2013-04-17
江苏华晟知识产权运营有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no efficient resource utilization method for waste activated sludge from fuel ethanol production plants.

Method used

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  • Cellulase production bacillus licheniformis with phosphate-solubilizing and potassium-releasing function and application of same
  • Cellulase production bacillus licheniformis with phosphate-solubilizing and potassium-releasing function and application of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Screening and isolation of Bacillus licheniformis BL-1CCTCC M 2012457

[0022] In May 2011, 7 soil samples were collected from forest lands in Henan, Anhui, and Jiangsu, and they were used as a plate culture medium with carboxymethyl cellulose as the sole carbon source (the composition of the medium is as follows by weight percentage: carboxymethyl cellulose Sodium cellulose 1.0%, KH 2 PO 4 0.1%, 0.3% tryptone, 0.1% yeast extract, 0.2% Congo red aqueous solution with a mass concentration of 2%, 2.0% agar, and the remaining components are water) for separation. The separation method is a typical dilution coating method for microorganisms, and the Cultivate overnight in an incubator at ℃; determine the cellulase-producing ability of the strain according to the size of the transparent circle produced by the colony grown on the medium, and finally screen to obtain a strain with the strongest cellulase-producing ability (the largest transparent circle) .

Embodiment 2

[0023] Example 2 Identification of Bacillus licheniformis BL-1CCTCC M 2012457

[0024] 1. Morphological characteristics of bacterial colonies

[0025] After culturing on LB solid medium at 37°C overnight, a round colony with irregular edges and hairy shape is formed, and the bacterial lawn is off-white, dry, opaque, and spreading; it is Gram-stained positive by optical microscope observation, and the bacteria The shape is helper-like, and the spores formed are oval and slightly longer.

[0026] 2. Physiological and biochemical characteristics

[0027] Physiological and biochemical identification was carried out on the cellulase-producing strain isolated in Example 1, and its main physiological and biochemical characteristics are shown in Table 1.

[0028] Table 1

[0029] Pilot projects

result

Spore shape

oval, slightly longer

Cell morphology

rod-shaped

hydrolyzed casein

+

Hydrolyzed starch

+

VP response

...

Embodiment 3

[0034] Example 3 Determination of Phosphorus and Potassium Ability of Bacillus licheniformis BL-1CCTCC M 2012457

[0035] 1. Determination of Phosphorus Solubilizing Ability

[0036] Use culture medium (the medium composition is calculated in g / L: glucose 10, ammonium sulfate 1.0, sodium chloride 0.1, magnesium sulfate 0.5, calcium carbonate 0.5, yeast powder 0.5, phosphate rock powder 1, add water to 1L, adjust pH to 7.2~7.5); Inoculate Bacillus licheniformis BL-1CCTCC M2012457 of the present invention into the above-mentioned medium according to the inoculum amount of 1% by weight, and cultivate it at 37° C. for 14 days; use the molybdenum-antimony anti-colorimetric method to measure the phosphorus in the culture liquid For the total amount of elements, a blank control (not inoculated with the strain of the present invention) was set at the same time, and the parallel determination was performed twice. The test results showed that the two parallel determinations of phospho...

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Abstract

The invention relates to cellulase production bacillus licheniformis with phosphate-solubilizing and potassium-releasing functions and the application of the cellulase production bacillus licheniformis. The invention discloses the bacillus licheniformis BL-1 with the preserving number of CCTCC No. M 2012457 and the bacterial strain can produce cellulose, which has an excellent phosphate-solubilizing and potassium-releasing function and can be made into composite probiotics through scientific formulation to microzyme, bacillus subtilis, bacillus megatherium, bacillus mucilaginosus and azotbacteria chroococum, and microbial active sludge organic fertilizer added with the composite probiotics in proportion is directly applied to plant growth regions or applied to plant growth regions after compost fermentation, and has excellent soil enriching and yield increasing effects. According to the invention, efficient resource comprehensive utilization to active sludge discarded by fuel ethanol manufacturers can be achieved, meanwhile, environment pollution is avoided, the treatment process is simple, and the application cost is low. Therefore, ecological protection and sustainable development to agriculture can be facilitated.

Description

technical field [0001] The invention belongs to the field of microbial technology and microbial fertilizers, and in particular relates to a cellulase-producing Bacillus licheniformis with the function of decomposing phosphorus and potassium, a high-efficiency microecological preparation containing the Bacillus licheniformis, and the microecological preparation in improving fuel efficiency. Application of industrial waste activated sludge for ethanol production on fertilizer effect. Background technique [0002] Chemical fertilizers are indispensable fertilizers in agricultural production. They have occupied a dominant position in the field of fertilizers for nearly a hundred years and have made great contributions to agricultural production. However, the long-term use of chemical fertilizers will not only reduce the quality of crops, cause soil compaction and other phenomena, but also cause environmental pollution, which is not conducive to the sustainable development of agr...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C05F11/08C05F7/00C12R1/10C12R1/125C12R1/11C12R1/07C12R1/065
CPCY02A40/20
Inventor 张梁张凌杰张君胜
Owner 江苏华晟知识产权运营有限公司
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