416 results about "Azotobacter chroococcum" patented technology

The invention discloses a compound probiotics preparation for biological organic fertilizers and a preparation method thereof. The preparation method comprises the following steps: performing activated culture of 14 kinds of probiotics, including Pseudomona putida, Streptomyces violovariabilis, Rhizobium sp., Azotobacter chroococcum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis, Bacillus weihenstephanensis, Bacillus mucilaginosus, Bacillus thuringiensis, Saccharomyces rouxii, Streptomyces griseus var. ferrugineus, and Streptomyces microflavus; performing mixed inoculation at a proportion of 1% to 5% to a 500 mL liquid seed culture medium; then performing amplification culture; and finally delivering the strains to a 500 mL fermentor and fermenting for 3 to 5 days under such conditions that the temperature ranges from 28 DEG C to 30 DEG C and the rotational speed is 200 rpm, to obtain a liquid-state compound probiotics preparation. The compound probiotics preparation for biological organic fertilizers, provided by the invention, plays important roles of taking advantage of microbial ecology, improving soil, promoting plant growth, improving the yield and disease resistance of crops and reducing the consumption of fertilizers.

The invention discloses a method to prepare leaven of fertilizer and application, which is characterized by the following: allocating raw material with mass ratio at 10-80 wt industrial residue of ferment, 5-30 wt straw, 5-30 wt cake meal, 10-30 wt bran or rice bran, 1.5-2 wt aquacare, 0.5-1 wt NaCl and or amylaceum, and 1-30 wt brown ball Azotobacter (Azotobacter chroococcum), 1-30 wt bacillus megaterium (Bacillus megaterium), 1-30 wt bacillus licheniformis (Bacillus licheniformis), 1-30 wt plant lactobacillus (Lactobacillus plantarum) and 1-30 wt 1-5 strain of saccharomycopsis fibuligera (Saccharomycopsis fibuligera); producing the product.

The invention relates to a compound microbial fertilizer and a preparation method thereof. The compound microbial fertilizer is prepared by mixing the following raw materials: yeast fermentation powder residues, an organic fertilizer, urea, K2SO4, NH4H2PO4, clay and a compound microbial agent; and the compound microbial agent is formed by fermentation of bacillus mucilaginosus, bacillus megaterium, bacillus cereus, bacillus thuringiensis, azotobacter chroococcum, pichia farinose and streptomyces microflavus. The compound microbial fertilizer disclosed by the invention has a variety of microbes with the effects of nitrogen fixation, phosphorus dissolution and potassium dissolution, can increase the nitrogen content in soil, improves the utilization rate of the chemical fertilizer, and is favorable for improving the structure of the soil and improving the yield and quality of crops; and simultaneously, the microbes in the fertilizer can produce a variety of metabolites, and the metabolites can effectively resist diseases and insect pests.

The invention discloses complex microbial inoculant which comprises the following active ingredients: bacillus subtilis, bacillus megatherium, trichoderma koningii, aspergillus oryzae, trichoderma viride, geotrichum candidum, aspergillus niger, rhizopus oligosporus, lactobacillus plantarum CICC20768 and azotobacter chroococcum. Correspondingly, the invention further discloses a method for preparing an organic fertilizer from the complex microbial inoculant. The method comprises the following steps: a. uniformly mixing the complex microbial inoculant with a plant fiber raw material to obtain a fermented raw material; b, composting and fermenting the fermentation raw material; c, drying the composted fermentation raw material to obtain the organic fertilizer. microbes of the complex microbial inoculant have no antagonism, and can effectively cooperate in the production process of the organic fertilizer, and the chemical components of the production raw materials can be sufficiently utilized to finally produce the high-quality organic fertilizer. The produced organic fertilizer reaches the parameter indexes of the standard (NY884-2012) of China, can improve the planting property of soil, is beneficial to optimization of soil microbial flora, and can provide comprehensive nutrients to a plant root system.

The invention relates to a liquid microbial fertilizer with nitrogen-fixing, phosphorus-dissolving and potassium-dissolving effects, and a preparation method thereof. The liquid microbial fertilizer consists of the following microbial fermentation liquids in parts by volume: 1 to 10 parts of azotobacter chroococcum, 1 to 30 parts of bacillus mucilaginosus, 1 to 10 parts of phosphorus-dissolving bacillus megatherium, 1 to 10 parts of bacillus amyloliquefaciens, 1 to 10 parts of bacillus subtilis, 1 to 5 parts of photosynthetic bacteria and 1 to 10 parts of streptomyces microflavus. The functional bacteria serve as main functional flora of the microbial fertilizer, so that the obtained product has remarkable effects of fixing nitrogen, dissolving phosphorus, dissolving potassium and resisting disease, can enhance the total metabolic activity of soil bacterial community and the diversity of the bacterial community, improve the soil micro-ecological environment, promote growth and reproduction of beneficial microorganisms and inhibit the growth of pathogenic bacteria, is an ideal fertilizer in green agriculture and organic agriculture, and has a wide prospect in agricultural sustainable development.

The invention discloses a microbial preparation for gangue reclaimed soil. The microbial preparation comprises three microbial preparations which are respectively used independently, wherein the I type microbial preparation contains bacillus subtilis and bacillus licheniformis; the II type microbial preparation contains bacillus mucilaginosus and the bacillus licheniformis; the III type microbial preparation contains azotobacter chroococcum, bacillus megaterium, the bacillus mucilaginosus and lysine bacillus; the total viable count of every gram of the microbial preparation is (2-200)*10<8> CFU. The gangue reclaimed soil is covered in layers by using the microbial preparation disclosed by the invention, a first gangue layer, a sticky loess and fly ash mixing covering layer, a second gangue layer, a fly ash covering layer, a fine granular gangue layer and a loess covering layer are sequentially arranged from bottom to top, the I type microbial preparation is applied to the sticky loess and fly ash mixing covering layer, the II type microbial preparation is applied to the fly ash covering layer, the III type microbial preparation is applied to the loess covering layer, and crops are planted on the loess covering layer.

The invention provides a functional bactericide for preventing and treating root-knot nematode.The functional bactericide comprises an azotobacter bactericide, a biocontrol bacterium bactericide and a carrier, and the mass ratio of the azotobacter bactericide to the biocontrol bacterium bactericide to the carrier is (1-2.5):(1.5-5):1.The azotobacter bacterium bactericide contains nodule bacteria and azotobacter chroococcum, and the living bacterium count ratio of nodule bacteria to azotobacter chroococcum is (1-100):(1-200).The biocontrol bacterium bactericide contains paecilomyces lilacinus, verticillium chamydosporium, bacillus subtilis and bacillus cereus, and the living bacterium count ratio of paecilomyces lilacinus to verticillium chamydosporium to bacillus subtilis to bacillus cereus is (1-100):(1-100):(1-100):(1-100).The living bacteria count of the functional bactericide is larger than 0.2 billion/g.When applied to fertilizer, the functional bactericide can improve the disease preventing effect and the growth promoting effect of microbial fertilizer, guarantee nutrition supply of microbial fertilizer in the applying process, achieve the effects of preventing and treating root-knot nematode, forming healthy root nodules and promoting plant growth, increase the yield of crops and improve the quality of crops.

The invention provides a microbial organic fertilizer and a preparation method thereof. The preparation method comprises the steps of strain culture, compounding and liquid submerged fermentation, and belongs to an agricultural integrated production technique. 15-20% of Bacillus mucilaginosus, 15-18% of Bacillus megaterium, 15-18% of Azotobacter chroococcum, 10-13% of Saccharomyces cerevisiae, 10-13% of Candida utilis, 15-18% of Lactobacillus plantarum and 10-13% of actinomycete are respectively subjected to primary and secondary strain culture and tertiary liquid submerged fermentation until the bacterial count in the fermentation liquid reaches 10-15*10<8>/mL, thereby obtaining the microbial organic fertilizer (707(Y)). By adopting the advanced liquid submerged fermentation technique and using the strains with high stability and functionality, which are screened from the natural world over the years and have the advantages of many varieties and convenient application method, the invention can promote the plant growth, improve the fruit quality, promote prematurity, increase the yield, enhance the utilization ratio of the fertilizer, improve the ecological environment of soil, and have certain effects of disease prevention and disease resistance.

The invention relates to a liquid composite biological multi-control fertilizer. The invention is characterized in that the liquid composite biological multi-control fertilizer is prepared from the following raw materials in parts by weight: 1-3 parts of Streptomyces hygroscopicus solution, 1-3 parts of Streptomyces cinnamofuscus solution, 2-4 parts of Bacillus amyloliquefaciens solution, 2-4 parts of bacillus sphaericus solution, 7-15 parts of Bacillus mucilaginosus solution, 1-3 parts of Azotobacter chroococcum solution, 1-3 parts of Streptomyces aureochrogmoenes, 8-10 parts of Trichoderma viride fermentative degradation solution, 8-10 parts of matrix and 45-65 parts of sterilized water. The invention prepares a crop-absorbing carbohydrate and organic matter Trichoderma viride fermentative degradation solution, and provides high-efficiency beneficial microbial florae with phosphate solubilizing, potassium solubilizing, nitrogen fixation and disease resistance effects. The fertilizer can simultaneously perform the three functions of biological pesticide, biofertilizer and biological nutrients, and has the advantages of no toxicity or harm and no environment pollution. Compared with other fertilizers with the same nutrients, the crop yield is increased by 20%.

The invention discloses a solid composite biological multi-control water and moisture retention fertilizer and a preparing method and application thereof.The fertilizer is prepared from bentonite, matrix, streptomyces hygroscopicus liquid, bacillus megatherium liquid, bacillus amyloliquefaciens liquid, bacillus sphaericus liquid, bacillus mucilaginosus liquid, azotobacter chroococcum liquid, bacillus cereus liquid, bacillus subtilis liquid, golden streptomyces chromogenes liquid, streptomyces microflavus liquid and trichoderma viride liquid.The preparing method comprises the steps of 1, preparing bacterial liquid; 2, mixing and drying the different kinds of bacterial liquid and the matrix, and then mixing with bentonite for pelletizing.The fertilizer can achieve nitrogen fixation, dephosphorization and potassium release, absorb water to maintain soil moisture, promote soil organic matter decomposition, resist bacteria and insects, improve microbiota in soil and improve the structure of soil.

The invention provides a novel compound microbial fertilizer containing Pulullan and polyglutamic acid and a preparation method of the novel compound microbial fertilizer. The compound microbial fertilizer comprises a microbial fermentation inoculum, compound nutrients, Pulullan and polyglutamic acid, wherein the microbial fermentation inoculum is prepared by mixing fermentation liquors of bacillus megatherium, bacillus amyloliquefaciens, bacillus thuringiensis, bacillus mucilaginosus and azotobacter chroococcum according to the mass ratio of 1: 1: 1: 1: 1; the compound nutrients comprise nitrogen, phosphorus, potassium, trace elements, organic matters and compound amino acids. By applying the microbial fertilizer, the bud ratio, the plant height and the yield of highland barley can be obviously improved, and the highland barley is excellent in taste. Furthermore, the use level of irrigation water can be reduced by 10% due to the film-forming property of Pulullan and the water-retaining property of polyglutamic acid. In addition, Pulullan and polyglutamic acid are mixed to form a liquid mulch on the surface of soil, so that the use of a plastic thin film can be reduced, an environmental protective role can be played, the labor force is alleviated, and the cost is lowered by 10-20%.

The invention discloses a bioanalysis preparation technique of a biological organic fertilizer by chicken manure. High-efficient and green chicken mature fermenting biological organic fertilizer is developed through a mixed bacteria aerobiotic two-step fermenting technique, wherein an one-step fermenting flora comprises aspergillus, thermophilic sporotrichum, saccharomycetes, bacillus subtilis, thermophilic heterotrophism ammonia oxidizing bacteria; a two-step aerobiotic fermenting flora comprises EM fungicide, azotobacter chroococcum, and bacillus licheniformis. The technique adopts multi-bacterium mixing fermentation; through combination of metabolic capabilities, the complex metabolism which is hard to complete by single culture is completed; through synergic fermentation between microorganisms, the chicken mature is effectively transformed, the hygienic index reaches the national standard, and the product decomposition and growth promoting index are significantly promoted. The fermenting technique is simple and high-efficient, the deodorization and sterilizing effects are good; besides, the production cost is low, and the technique is easy to carry out industrial scale production.

The invention relates to a microbial fertilizer and a preparation method thereof. The preparation method is as follows: bacillus cereus, bacillus megaterium and bacillus subtillis are mixed and fermented; bacillus mucilaginosus, pantoea agglomerans and azotobacter chroococcum are fermented respectively; the four fermented cultures are mixed and filled according to a certain proportion so as to produce a microbial blend which is used by the methods of seed soaking, root irrigation and spraying. The microbial fertilizer can promote plant rhizosphere growth and propagation, improve soil, eliminate soil hardening, enhance soil permeability and promote the development of the root system; the microbial fertilizer has the advantages of releasing potassium and phosphorus, fixing nitrogen, improving fertilizer efficiency and reducing the amount of chemical fertilizer used, inhibiting the propagation of harmful bacteria in the soil, preventing the diseases such as soil-borne diseases, replant disease and the like; thus realizing the functions of increasing income and improving the quality of agricultural products.

The invention discloses aerobic bacterium-anaerobic bacterium composite microbe liquid inocula and a preparation method thereof. The composite microbe liquid inocula comprise six liquid aerobic microbial agents and four liquid anaerobic microbial agents. The liquid aerobic microbial agents comprise a bacillus megaterium agent, a liquid Azotobacter chroococcum agent, a liquid bacillus mucilaginosus agent, a liquid streptomyces microflavus agent, a liquid bacillus subtilis agent and a liquid bacillus licheniformis agent. The liquid anaerobic microbial agents comprise liquid photosynthetic bacteria, a liquid lactobacillus plantarum agent, a liquid lactobacillus acidophilus agent and a liquid lactobacillus delbrueckii agent. The preparation method comprises culturing six types of aerobic bacteria to obtain six liquid aerobic microbial agents, culturing four types of anaerobic bacteria to obtain four mixed liquid anaerobic microbial agents, and injecting the six liquid aerobic microbial agents into the mixed liquid anaerobic microbial agents to obtain the composite microbe liquid inocula. The composite microbe liquid inocula have a quality guarantee period for one year or more at the normal temperature and have functions of nitrogen fixation, phosphate dissolution, potassium release, disease prevention and soil improvement.

The invention discloses a complex bacterium preparation for fixing nitrogen, solubilizing phosphorus and potassium and rooting and a preparation method thereof. The preparation is mainly prepared by mixing the following raw materials in parts by weight: 5-20 parts of azotobacter vinelandii, 10-30 parts of bacillus megatherium, 10-30 parts of bacillus mucilaginosus, 5-15 parts of azotobacter chroococcum, 10-15 parts of glomus intraradices and 10-25 parts of glomus mosseae. The preparation method comprises the following steps: respectively carrying out high-density culture on each strain; grafting to a shake flask filled with a culture medium in a volume ratio of 15-30 percent for activated culture; grafting the cultured strains into a fermentation tank for fermentation culture expansion; dehydrating and drying the culture expanded single strains to prepare hypopus microorganism dried powder; and mixing to obtain the complex bacterium preparation for fixing nitrogen, solubilizing phosphorus and potassium and rooting. The complex bacterium preparation is used for industrial crops, fruit trees, food crops and the like, has the advantages of biological property, environmental friendliness, high yield and high quality in comparison with existing products, and cannot pollute soil.

The invention discloses a composite organic fertilizer prepared from vegetable wastes. The composite organic fertilizer is a fermented product which is obtained by fermenting a composite microbes and the vegetable wastes, wherein the composite microbe is a mixture of bacillus mucilaginosus, bacillus subtilis, tiny bacillus, bacillus megatherium, pseudomonas aeruginosa, plant lactic acid bacteria and azotobacter chroococcum, the number ratio of all the strains is 1:(7-8):(4-6):(3-5):(1-3):(3-5):(1-2), and the total amount of viable bacteria is 2.0*10<9>-3.0*10<9> CFU/mL. The invention also discloses a preparation method of the composite organic fertilizer. The preparation method comprises the following steps: inoculating the microbes to prepare a culture medium; and adsorbing by using an adsorbent, and fermenting the vegetable wastes to obtain compost. The compost prepared by the preparation method disclosed by the invention can be used for promoting the growth development of plants, and reducing plant diseases and insect pests and reducing the use amount of the inorganic fertilizer, so that the vegetable wastes are effectively used, and pollution of wastes to farm environments is reduced.

The invention relates to a drip-irrigation type salt stress-alleviating and disease-preventing biological bacterial fertilizer and a preparation method thereof. The drip-irrigation type salt stress-relieving and disease-preventing biological bacterial fertilizer comprises nutrition carriers, additives, microelements and functional microorganisms, wherein the nutrition carriers comprise two or more of humic acid, glutamic acid, urea, corn flour, potassium sulphate, potassium chloride and diammonium hydrogen phosphate; the additives comprise two or more of bentonite, sodium carboxymethyl cellulose, sodium alginate an polyvinyl alcohol; the microelements comprise one or two of boric acid an ammonium molybdate; and the functional microorganisms is formed by combining salt stress-alleviating growth-promoting bacteria and disease-preventing growth-promoting bacteria, wherein the salt stress-alleviating growth-promoting bacteria is one or two selected from pseudomonas putida and azotobacter chroococcum; and the disease-preventing growth-promoting bacteria is one or two selected from bacillus subtilis and bacillus megatherium. The biological bacterial fertilizer is in a liquid dosage form, has relatively high effective viable count and relatively long quality guarantee period. The effective viable count of the biological bacterial fertilizer is higher than or equal to 10<10> cfu/mL; and total nutrient content of (N+P2O5+K2O) is higher than or equal to 12%.

The invention discloses high-concentration compound microorganism bacterial fertilizer and a production method thereof, wherein the production method comprises the following steps: respectively cultivating azotobacter chroococcum, bacillus megatherium, bacillus mucilaginosus, bacillus licheniformis and bacillus macerans to obtain seeds for fermentation; sterilizing a production culture medium in a fermentation tank at the temperature of 121 DEG C for 20 minutes, inoculating strain seeds when the temperature of the culture medium decreases to about 33 DEG C, fermenting for 24 to 30 hours at the temperature of 33+/-2 DEG C under the condition of introducing oxygen to obtain the compound microorganism bacterial fertilizer, wherein the number of effective live bacteria is larger than or equal to 5*109 (50 hundred million)/mL, and the number of each kind of bacteria is not lower than 3 hundred million/mL. The method adopted by the invention is scientific and reasonable, and has simple and easy operation steps, strong industrialized feasibility and important meanings for promoting agricultural production.

The invention relates to a biological organic fertilizer used for aquaculture paddy fields and the production method thereof. The fertilizer is prepared by the raw materials according to the parts by weight: 0.3 to 0.5 parts of taurine, 2 to 3 parts of treptomyces hygroscopicus, 2 to 3 parts of chromagenic streptomyces aureofaciens, 2 to 4 parts of bacillus cereus, 2 to 4 parts of bacillus sphaericus, 9 to 13 parts of bacillus mucilaginosus, 2 to 3 parts of azotobacter chroococcum, 2 to 3 parts of streptomyces microflavus, 10 parts of fruit waste degradation products, 10 parts of matrix and 46.5 to 57 parts of soil. In the method, as fruit waste degradation product polysaccharide carbohydrate is mixed with nitrogen-fixing, phosphate-dissolving and potassium-dissolving active compound microorganisms and a compound actinomycete agent which has antagonistic effect on rice blast and rice sheath blight, and matched with nutritional components which stimulate the growth of fisheries in rice fields, the biological organic fertilizer can not only be used for improving soil microbial community structure, activating soil nutrients, and improving rice rhizosphere nutrition, increasing the yield and quality of rice and improving water quality, but also promote the growth of cultured fisheries in rice fields.

The invention relates to a composite microbial preparation for promoting the growth of rape and a preparation method thereof. The composite microbial preparation for promoting the growth of the rape is characterized by comprising 20-40 parts by weight of strain culturing substance and 60-80 parts by weight of microbial carrier, wherein the strain culturing substance comprises the following raw materials in parts by weight: 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium, 5-10 parts of rhodopseudomonas palustris and 1-4 parts of azotobacter chroococcum. The preparation method of the composite microbial preparation for promoting the growth of the rape comprises the following steps: culturing 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium and the rhodopseudomonas palustris together, culturing the azotobacter chroococcum independently, mixing and fermenting. The invention has the advantages of promoting the growth and the propagation of beneficial microorganisms, increasing the absorption of the root system of the rape to nitrogen, phosphorus, potassium and other organic substances and promoting the normal development of the root system, thus promoting the growth of the rape and reducing the applied chemical fertilizer.

The invention discloses a nano-carbon organic compound fertilizer, which consists of proper parts by weight of urea, potassium sulfate, hairs, palygorskite, dimethyl sulfoxide, a mixture consisting of a boron fertilizer and a zinc fertilizer in the weight ratio of 1:0.5, ferrous sulfate, nodule bacteria, azotobacter chroococcum, nano-carbon, curculigo orchioides, golden larch bark, light yellow sophora root, ligninase, plant ash, humic acid, sodium dodecyl benzene sulfonate, animal dung and powdery EDTA (Ethylene Diamine Tetraacetic Acid) manganese chelate. The nano-carbon organic compound fertilizer contributes to effective utilization of secondary and trace elements; the nano-carbon organic compound fertilizer is suitable for crops such as wheat, paddy rice, corn, soybean and oilseed rape, and the cold resistance, drought resistance and disease and insect resistance can be enhanced; when the nano-carbon organic compound fertilizer is applied to cotton and hemp crops, the tension and strength of fibers can be enhanced, the yield is increased remarkably, and the quality is improved remarkably.

The invention relates to Bacillus subtilis BC-198, and a selenium-rich microbial inoculum and application thereof. The collection number of the Bacillus subtilis BC-198 is CCTCC No.M2012321. The invention also provides a composite microbial inoculum of which the effective ingredients are the Bacillus subtilis BC-198 and Azotobacter chroococcum AS1.212. The preparation method of the selenium-rich microbial inoculum provided by the invention can utilize the existing universal fermentation plant, is simple to operate and has the advantage of low manufacturing cost; the prepared product has multiple functions of nitrogen fixation, growth promotion, fungus disease control and the like for crops; and after being applied to the crops, the selenium-rich microbial inoculum can increase the yield, improve the quality of the agricultural product, and enhance the selenium content in the agricultural product by more than 20 times, so that the agricultural product reaches the selenium-rich product quality standard.

The invention relates to fertilizer pesticide, and specifically relates to an organic fertilizer fermenting agent and a preparation method thereof. The fermenting agent is composed of bacillus subtilis, bacillus mucilaginosus, trichoderma viride, lactococcus lactis, actinomycetes, azotobacter chroococcum, bacillus megatherium, bacillus mucilaginosus, escherichia coli, active ingredient, cellulose, protease and phosphorylase. The preparation method of the fermenting agent comprises the following steps: 1, weighing each constituent; 2, preparing a mixture I; 3, preparing a mixture II; 4, smashing. According to the invention, active ingredient is added into the fermenting agent, so that effective viable count content in the fermenting agent is improved greatly, heating and fermenting time is reduced, and absorption and utilization rates of the fermenting agent by poultry dung, straw, and the like, are improved. Aspergillus niger in the active ingredient is uniformly stirred with other constituents after being stirred well with orangeade, thus, absorption and utilization of the organics in the active ingredient by various microbes in the fermenting agent are promoted greatly, and the increase of effective viable count in the fermenting agent is facilitated.