29 results about "Azotobacter chroococcum" patented technology

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention relates to the technical field of microorganisms, and particularly discloses a nitrogen-fixing and cadmium-reducing microbial agent as well as a preparation method and application thereof. The microbial agent consists of bacillus pseudomycoides, lactococcus lactis, azotobacter chroococcum and biochar, wherein the biochar is obtained by pyrolyzing and carbonizing rice straws. The bacillus pseudomycoides can reduce the bio-availability of soil cadmium; lactococcus lactis can secrete a large number of biological active substances such as exopolysaccharides and enzymes, and the activity of other microorganisms is stimulated and improved; azotobacter chroococcum converts nitrogen in air into ammonium, amino acid and peptide which can be utilized by plants and promotes plant growth, and released metallothionein contains free sulfydryl which is combined with Cd ions, so that a stable sulfide compound is formed. The biological carbon adsorbs and fixes the three bacteria, so that the microorganisms can continuously and effectively play a role and have a mutual synergistic effect, the fixation of Cd in the soil is further enhanced, the availability of nitrogen is improved, and the plant growth is effectively promoted.

The invention discloses a salinity regulation and control seedling culture method for hibiscus syriacus, and belongs to the technical field of hibiscus syriacus seedling culture. The method comprises the following steps: (1) seed treatment; (2) preparing a substrate; (3) after sowing, culturing in a greenhouse; (4) transplanting and salinity control: a) when the seedlings grow to have 3-4 true leaves, transplanting the seedlings into a nutrition bag, adding 0.4-0.6 wt% of a microbial liquid into the matrix at the same time, the microbial liquid containing nitrogen-fixing bacteria Azobacter chrocococcus and phosphate-solubilizing bacteria Bacillus licheniformis, and the total number of viable bacteria being greater than or equal to 1 * 10 < 9 > CFU / mL; after transplanting, carrying out drip irrigation with brackish seawater, and spraying a 0.1% monopotassium phosphate solution on leaf surfaces every half a month for three times in total; b) directly carrying out drip irrigation on brackish seawater in the nutrition bag; and (5) outdoor cold-resistant treatment: 30 days before winter, spraying 0.2-0.5 L of 0.2-0.4 wt% potassium sulfate aqueous solution to leaf surfaces for each plant, spraying once every 6-8 days, and spraying 3-5 times in total. Through salinity gradient domestication and use of the microbial agent, the biomass loss rate of the hibiscus syriacus under the salinity of 0.5% is remarkably reduced, and the annual increment of the plant height is improved.

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for heavy metal contaminated soil and a biological bacterial fertilizer and a preparation method thereof. The micro-ecological restoration liquid complex microbial inoculant suitable for the heavy metal contaminated soil comprises nitrogen-fixing bacteria: azotobacter chroococcum ACCC 11104, phosphate solubilizing bacteria: pseudomonas fluorescens ACCC 03031, potassium solubilizing bacteria: bacillus mucilaginosus CCTCC KB20082790, growth-promoting bacteria: azotobacter braziliensis CCTCC AB 91001, bacillus subtilis CGMCC 17161, synergistic bacteria: trichoderma asperellum CGMCC 3.17461, and restoration bacteria: pseudomonas putida CGMCC 1.4533. The biological bacterial fertilizer for the heavy metal contaminated soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the heavy metal contaminated soil is greatly improved, the heavy metal contaminated soil is effectively improved, and the crop yield is remarkably increased.

The invention belongs to the technical field of biological bacterial fertilizers, and particularly relates to a high-salt-tolerance microbial liquid complex microbial inoculant suitable for saline-alkali desertification land and a biological bacterial fertilizer thereof. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation of three stages: salt-tolerant repair bacteria Nocardia halophila, salt-tolerant functional bacteria Bacillus subtilis and Azospirillum brasilense, nitrogen-fixing functional bacteria Azotobacter chroococcum, phosphorus-solubilizing functional bacteria Pseudomonas fluorescens, and nitrogen-solubilizing functional bacteria Pseudomonas fluorescens. The bacillus mucilaginosus is used as a potassium-dissolving functional bacterium, the trichoderma asperellum is used as a biocontrol growth-promoting bacterium, and the trichoderma harzianum is used as a rhizosphere optimizing bacterium. The biological bacterial fertilizer is prepared from the liquid complex microbial inoculant. Through scientific compatibility of different strains provided by the invention, efficient improvement and crop yield increase of the saline-alkali desertification land are realized, and the strain has important economic and social values.

The invention discloses an oil shale waste residue microwave activation and microorganism coupling soil improvement method which comprises the following steps: crushing and screening oil shale dry distillation residues, and adding ground phosphate rock when necessary; then carrying out two-stage microwave activation treatment to obtain an activated waste residue carrier with high specific surface area and low content of harmful substances; mixing the activated carrier with a compound microbial agent (containing azotobacter chroococcum, silicate bacteria and cellulose degrading bacteria) and straws according to a specific ratio, and carrying out stack retting to prepare a harmless activated waste residue modifier; finally, the application amount is determined according to the soil type, and the fertilizer is applied to soil. According to the invention, harmlessness and high-value recycling of the oil shale waste residues are realized, and the prepared modifier can significantly increase the soil nutrient content, improve the soil structure, adjust the pH value and promote crop growth, is suitable for improvement of various degraded soil, has the advantages of efficient process, low cost, environmental friendliness and the like, and has a relatively high popularization value.

The invention discloses a hydrogel and probiotic compounded microbial inoculum composition and a vegetable planting matrix, a three-dimensional gel carrier is used for embedding a microbial inoculum, the carrier integrates mechanical properties and thallus protection and can maintain flora activity for a long time, bacillus thuringiensis has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression, and the microbial inoculum composition has the advantages that the microbial inoculum composition has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression; the microbial agent composition can survive for a long time in a low-oxygen environment of a carrier embedding environment, and is cooperated with bacillus cereus, azotobacter chroococcum and bacillus megatherium to promote the growth of plants such as vegetables and the like and directionally kill pathogenic microorganisms such as root-knot nematode. Corresponding pests can be killed in a green and targeted manner, the growth of plants is promoted, and the yield is increased.

The invention relates to the technical field of biology, and discloses a recombinant N-acetylglucosamine-1-phosphate uridine transferase (GlmU) mutant and a preparation method thereof. The sequence of the recombinase is formed by connecting an N-acetylglucosamine-1-uridine phosphate transferase (GlmU) mutant (L113V-V172I) from Azotobacter chroococcum, an N-terminal histidine tag (His-tag) and a small ubiquitin-like modified protein tag (SUMO-tag) in series. The invention further discloses a preparation method of the recombinase. Compared with a recombinant wild type N-acetylglucosamine-1-uridine phosphate transferase with the same source, the recombinant N-acetylglucosamine-1-uridine phosphate transferase mutant has higher activity which is 1.5 times of that of the recombinant wild type and higher stability, and the residual activity of the mutant is 66.5% after the mutant is placed at room temperature for 5 days, so that the mutant has a good application prospect in the field of N-acetylglucosamine-1-uridine phosphate transferase. And the soluble expression quantity of the recombinant mutant N-acetylglucosamine-1-uridine phosphate transferase is increased by 5 times.

The invention provides a saline-alkali soil conditioner and a preparation method thereof. The preparation method comprises the following steps: S1, preparing polystyrene microspheres coated with polyethyleneimine; s2, preparing attapulgite of which the surface is grafted with TEMPO oxidized cellulose, and then carbonizing the attapulgite to obtain a carbon-coated attapulgite composite material; s3, preparing composite aerogel; s4, bacillus pasteurii and azotobacter chroococcum are loaded on the composite aerogel, and the saline-alkali soil conditioner is obtained. The double strains are loaded on the composite aerogel, controlled release and slow release of the strains are achieved, in addition, the two microbial agents serve as dominant flora, breeding of harmful pathogens in soil can be inhibited, soil micro-ecological balance can be adjusted, and a healthy rhizosphere environment is created for follow-up crop planting.

The application discloses nitrogen-doped porous carbon material and a preparation method and application thereof. The preparation method of the nitrogen-doped porous carbon material comprises the following steps: 1) after biomass waste is crushed, water is added to perform a hydrothermal reaction to obtain porous carbon particles; 2) azotobacter chroococcum is inoculated into an AzuBay nitrogen-free liquid culture medium, and then the porous carbon particles are added to perform biological nitrogen fixation to obtain the porous carbon particles loaded with microbial nitrogen; and 3) the porous carbon particles loaded with microbial nitrogen are placed in a water vapor-carbon dioxide-nitrogen mixed atmosphere to perform activation, and thus the nitrogen-doped porous carbon material is obtained. The nitrogen-doped porous carbon material is prepared by using the azotobacter chroococcum to take the nitrogen in the air as a nitrogen source, without adding an external nitrogen source, and the process is green and environmentally-friendly, and the preparation cost is low. Finally, the nitrogen-doped porous carbon material mainly containing pyrrole nitrogen as a nitrogen-containing functional group can be prepared, and the type of the nitrogen-containing functional group can be flexibly adjusted.

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for saline-alkali soil and a biological bacterial fertilizer of the micro-ecological restoration liquid composite microbial agent. The micro-ecological restoration liquid complex microbial inoculant suitable for the saline-alkali soil is prepared from restoration salt-tolerant bacteria, namely bacillus subtilis, nocardia halospora, nitrogen-fixing bacteria, namely azotobacter chroococcum, phosphate solubilizing bacteria, namely pseudomonas fluorescens, potassium solubilizing bacteria, namely bacillus mucilaginosus, growth-promoting bacteria, namely azospirillum brasilense, biocontrol bacteria, namely bacillus megatherium and synergistic bacteria, wherein the restoration salt-tolerant bacteria are bacillus subtilis, nocardia halospora, the nitrogen-fixing bacteria are azotobacter chroococcum, the phosphate solubilizing bacteria are pseudomonas fluorescens, the potassium solubilizing bacteria are bacillus mucilaginosus, the growth-promoting bacteria are azospirillum brasilense, the biocontrol bacteria are bacillus megatherium, and the synergistic bacteria are prepared from bacillus subtilis The biological bacterial fertilizer for the saline-alkali soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the saline-alkali soil is greatly improved, the saline-alkali soil is effectively improved, and the crop yield is remarkably increased.

The invention discloses Azospirillum brasilense capable of producing protease, decomposing potassium, degrading cellulose, promoting growth and relieving NaCl stress of plants and application of the Azospirillum brasilense. The invention provides nitrogen-fixing spirillum or a progeny thereof, the nitrogen-fixing spirillum is Dongying nitrogen-fixing spirillum, the strain number of the nitrogen-fixing spirillum is RS252, and the registration number of the nitrogen-fixing spirillum in the China General Microbiological Culture Collection Center is CGMCC (China General Microbiological Culture Collection Center) No. 31494. The strain is a new strain of azospirillum, and has the capabilities of producing protease, decomposing potassium and degrading cellulose. Pot experiments show that under the stress of NaCl, compared with an uninoculated negative control group, after the strain is inoculated, the root length and underground fresh weight of rice can be improved, and the strain can be used as a microbial organic fertilizer for improving soil fertility, relieving the NaCl stress of crops and improving the NaCl stress adaptability of the crops.

The present application discloses a strain of azotobacter chroococcum with the abilities of producing protease, decomposing potassium and cellulose, promoting growth and relieving plant NaCl stress, and application thereof. Azospirillum dongyingense The present application provides the azotobacter chroococcum or its offspring, the azotobacter chroococcum is azotobacter chroococcum (Azotobacter chroococcum) of Dongying, the strain number is RS252, and the registration number of the Center of General Microbiological Culture Collection of China Microbial Culture Collection Committee is CGMCC No. 31494. The strain is a new strain of azotobacter chroococcum genus, has the abilities of producing protease, decomposing potassium and cellulose. Potted test shows that compared with the negative control group without inoculation, the strain can improve the root length and underground fresh weight of rice under NaCl stress, and the strain can be used as microbial organic fertilizer to improve soil fertility, relieve crop NaCl stress and improve the adaptability of crops to NaCl stress.

The invention provides a lettuce growth-promoting microbial agent and a lettuce planting method based on agriculture and light complementation, and belongs to the technical field of vegetable planting. The lettuce growth-promoting microbial agent comprises bacillus subtilis, paenibacillus polymyxa, rhodopseudomonas palustris, Aureobasidium tenuissimum and Azotobacter chroococcum in a mass ratio of (1-3): (2-4): (2-4): (4-6): (1-3). The method comprises the following steps: soaking lettuce seeds in a seed soaking agent, airing, sowing on a seedling culture substrate, covering with soil, and culturing to obtain seedlings; ploughing the land under the photovoltaic panel, and applying a vegetable residue organic fertilizer; transplanting when 4-6 true leaves grow on the seedlings, and applying the lettuce growth-promoting microbial agent while transplanting; a nutrient solution is sprayed 7-10 days after transplanting and 15-20 days after transplanting. According to the lettuce planting method based on agriculture and light complementation, the photosynthesis and growth and development of lettuces under the photovoltaic panel are greatly improved, and the yield and quality of the lettuces are improved.

The invention discloses a preparation method of a healthy and environment-friendly household lawn planting fertilizer, and belongs to the technical field of agricultural fertilizers. The invention relates to a preparation method of a healthy and environment-friendly household lawn planting fertilizer. The preparation method comprises the following steps: 1) efficiently converting kitchen garbage and garden waste by adopting a complex microbial inoculant (azotobacter chroococcum, thermophilic cellulose decomposition bacteria and candida tropicalis) in a specific ratio; 2) designing an intelligent response type core-shell coating structure, and realizing accurate controlled release of nutrients through the synergistic effect of a PHBV copolymer core layer, a modified starch interface layer and a temperature-sensitive nano composite shell layer; and 3) a safe combination of a bitter agent and a botanical insecticide is innovatively used. The product has the characteristics of quick start and long-acting supply, can obviously improve the soil environment, is completely biodegradable and pollution-free, and solves the problems of single function and serious pollution of the traditional fertilizer. The preparation process ensures stable product performance by optimizing fermentation parameters and an electrospray coating technology, and is particularly suitable for ecological maintenance requirements of family lawns.

The invention relates to the field of microbial agents, in particular to a universal soil micro-ecological restoration liquid composite microbial agent suitable for industrial production and a biological nitrogen fertilizer thereof. Comprising six functional strains: nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium-solubilizing bacteria, growth-promoting bacteria, biocontrol bacteria and synergistic bacteria, the nitrogen-fixing bacteria are azotobacter chroococcum, the phosphate-solubilizing bacteria are pseudomonas fluorescens, the potassium-solubilizing bacteria are bacillus mucilaginosus, the growth-promoting bacteria are azotobacter braziliensis, the biocontrol bacteria are bacillus subtilis, and the synergistic bacteria are trichoderma asperellum; the liquid complex microbial inoculant is prepared from the six functional strains through liquid fermentation by using electrochemical low-Hertz water. The biological nitrogen fertilizer is prepared by using the liquid complex microbial inoculant, exerts the functions of nitrogen fixation, phosphate solubilization, potassium solubilization, growth promotion, biocontrol and synergy, also can reduce the dependence on chemical fertilizers, not only can improve the soil quality, but also achieves the synergy goals of crop yield increase, quality improvement and soil health.

The invention discloses a method for preparing vegetation planting soil by utilizing industrial solid waste and microorganisms and application of the vegetation planting soil. The method comprises the following steps: mixing 80-90% of high titanium slag, 7-15% of sandy soil or phosphogypsum and 2-5% of titanium gypsum into a vegetation matrix according to specific particle size grading; inoculating a composite strain consisting of bacillus mucilaginosus and azotobacter chroococcum, fermenting at 30 DEG C for 12-18 days, and dynamically monitoring the contents of alkali-hydrolyzable nitrogen, available phosphorus and potassium and biologically extractable titanium; then, alfalfa is planted, arbuscular mycorrhizal fungi and rhizobium suspensions with the OD600 being 0.8-1.0 are regularly irrigated, and curing is conducted for 12-18 days; and irrigating and maintaining for 12-18 days by using chlorella propagation liquid every 4-6 days, and finally reserving the whole plant or harvesting to obtain the plant growing planting soil. According to the method, industrial solid waste is efficiently converted into a functional planting medium through the multi-stage biological-mineral synergistic effect, solid waste recycling and soil function reconstruction are achieved, and the method has the environment restoration and ecological reconstruction value.

The invention relates to a fermentation process of a straw organic fertilizer, and belongs to the field of biological ferment.The fermentation process is characterized in that the fermentation period can be remarkably shortened through the synergistic effect of cellulose thermophilus, streptomyces thermophilus, bacillus subtilis, lactobacillus plantarum, azotobacter chroococcum and geobacillus stearothermophilus in a complex microbial inoculant in cooperation with a segmented fermentation technology, and the fermentation rate can be increased within 20 days; the fermentation is basically completed and the fermentation time is effectively saved. The straw returning machine can be widely applied to straw returning of rice, wheat, corn and the like.

The invention discloses a preparation method of a biogas slurry-based organic aqueous solution fertilizer, which comprises the following steps: filtering fresh anaerobic digestion biogas slurry, adding a dispersing agent, adjusting the pH value, mixing with a composite high-temperature bacterial agent, carrying out primary fermentation, carrying out secondary electrochemical treatment, adjusting the content ratio of nitrogen to phosphorus to potassium, adding a seaweed extract, a water-retaining agent and a preservative, and carrying out secondary electrochemical treatment to obtain the biogas slurry-based organic aqueous solution fertilizer. Uniformly mixing and curing to obtain an organic water solution fertilizer; wherein the composite high-temperature fungicide is prepared by compounding bacillus licheniformis, sporotrichum thermophile and azotobacter chroococcum according to the viable count ratio of 3: 1: 1. The high-end functional liquid fertilizer product with market competitiveness is prepared by efficiently treating biogas slurry through staged and multi-technology cooperation, decomposing organic matters and plant toxic substances and passivating metals.

The invention provides a simplified efficient cultivation method for facility figs. The method for preparing the biochar comprises the following steps that S1, corn straw is rubbed into shreds and cut into short shreds through a pulverizer, orange residues are pulverized, and then the corn straw and the orange residues are mixed to prepare a solid material; and S2, respectively fermenting azotobacter chroococcum and eurotium scherweri to prepare seed solutions, inoculating the seed solutions to the solid material for solid culture, and pyrolyzing the dried fermentation product to prepare the biochar. The simplified and efficient facility fig cultivation method comprises the steps of site selection planning, facility building, soil improvement and field planting and growth management. The prepared biochar is applied to fig cultivation soil, the fertility of the soil is remarkably improved, the use amount of chemical fertilizer is reduced, meanwhile, the treatment cost of agricultural waste is reduced, high-quality, high-yield and low-consumption production of figs is achieved, the economic benefits of fruit farmers are guaranteed, and sustainable development of the local fig industry is promoted.

The invention relates to the field of microbial agents, in particular to a soil micro-ecological restoration liquid composite microbial agent and biological bacterial fertilizer suitable for hillside dry land. The soil micro-ecological restoration liquid complex microbial inoculant suitable for the hillside dry land comprises nitrogen-fixing bacteria: azotobacter chroococcum ACCC 11104, phosphate solubilizing bacteria: pseudomonas fluorescens ACCC 03031, potassium solubilizing bacteria: bacillus mucilaginosus CCTCC KB20082790, growth-promoting bacteria: azospirillum brazil CCTCC AB 91001, bacillus subtilis CGMCC 17161, synergistic bacteria: trichoderma asperellum CGMCC 3.17461, and drought-resistant bacteria: bacillus megatherium ACCC 11099 and streptomyces CCTCC KA 2008039. The biological bacterial fertilizer for the hillside dry land is prepared from the liquid complex microbial inoculant, and compared with a conventional biological bacterial fertilizer, the biological bacterial fertilizer has the advantages that the ecological adaptability to the hillside dry land is greatly improved, the hillside dry land is effectively improved, and the crop yield is remarkably increased.

The invention relates to the technical field of organic fertilizer production, and particularly discloses a mink and fox manure microbial fermentation organic fertilizer and a preparation method thereof. Candida lipolytica, bacillus subtilis, lactobacillus plantarum, azotobacter chroococcum and saccharomyces cerevisiae are compounded according to the volume ratio of 3: 3: 2: 1: 1 to prepare a zymophyte solution, the manure of minks and foxes is mixed with oyster mushroom bran, chestnut shells or corn straw according to a specific proportion, fermentation is started at the temperature of 35 DEG C, and the total fermentation period is 20-30 days. According to the preparation method provided by the invention, the degradation efficiency of grease in feces of minks and foxes is remarkably improved, the nitrogen loss is reduced, the total nitrogen content of the obtained organic fertilizer is greater than or equal to 1.97%, the total phosphorus content is greater than or equal to 0.34%, the coliform group is less than or equal to 98.5 CFU / g, and the standards of NY 884-2012 and GB 8172-1987 are met.

The invention relates to a promoter, a carrier and a transformant for specific inducible expression of arginine secretions of a rice root system and application of the promoter, the carrier and the transformant. The promoter can promote the expression of a target gene in a host under the induction of arginine secretion of a rice root system, especially the expression in nitrogen-fixing pseudomonas stutzeri. The promoter, the vector and the transformant disclosed by the invention have wide application potential in the aspect of construction of an artificial efficient rhizosphere combined nitrogen fixation system.

The invention provides a soil biological conditioning agent for rapidly increasing the earthworm proliferation rate. The soil biological conditioning agent is prepared from a compound microbial agent, a functional enzyme preparation and a humic acid carrier containing calcium alginate, the compound microbial agent comprises bacillus amyloliquefaciens (Bacillus amyloliquefaciens), azotobacter chroococcum (Azotobacter chroococcum) and Saccharomyces cerevisiae (Saccharomyces cerevisiae), and the compound microbial agent is prepared from bacillus amyloliquefaciens (Bacillus amyloliquefaciens), azotobacter chroococcum (Azotobacter chroococcum) and Saccharomyces cerevisiae (Saccharomyces cerevisiae (Saccharomyces cerevisiae). The functional enzyme preparation comprises cellulase and chitinase. According to the conditioning agent disclosed by the invention, a ternary synergistic system consisting of the compound microbial agent, the functional enzyme preparation and the humic acid carrier containing calcium alginate is constructed, so that a natural soil ecological process can be simulated and enhanced, and whole-course efficient regulation and control on earthworm nutrition supply and environment improvement are realized.

The invention discloses an implementation method of a compound microbial agent in early field saline-alkali soil treatment, and belongs to the technical field of saline-alkali soil treatment. The method comprises the steps of preparation of a compound microbial agent, pretreatment of early field saline-alkali soil, application of the compound microbial agent and matched management measures. The compound microbial agent is prepared by mixing bacillus subtilis, bacillus licheniformis, bacillus megaterium, bacillus mucilaginosus and azotobacter chroococcum according to the volume ratio of 3: 2: 2: 2: 1. The early field saline-alkali soil pretreatment comprises deep ploughing and soil loosening and land leveling; the fungicide is applied in two times, the first time is spraying application 10-15 days before sowing, and the second time is root irrigation application in the crop seedling stage. Through the synergistic effect of various strains, the soil salinity and alkalinity can be effectively reduced, the soil nutrient effectiveness is improved, the soil structure is improved, the crop growth is promoted, the yield is increased, the cost is low, no pollution is caused, and good economic benefits and ecological benefits are achieved.

The application discloses an environment-friendly biological organic fertilizer for improving acidified soil and a preparation method thereof, and belongs to the technical field of organic fertilizer. The biological organic fertilizer is prepared from the following raw materials in parts by weight: 10-20 parts of composite gel material, 80-100 parts of mature poultry manure, 10-20 parts of microbial agent, 30-50 parts of modified waste tea leaf biochar, 10-15 parts of urea, 5-10 parts of humic acid, 1-5 parts of superphosphoric acid calcium, and 1-5 parts of sodium carboxymethyl cellulose; the microbial agent is prepared by mixing bacillus firmus, pseudomonas plecoglossicida and azotobacter chroococcum at a volume ratio of 1:1:1. The biological organic fertilizer can significantly improve the soil pH, increase the content of organic matter in the soil, has good buffering capacity and long-term effect, and can enhance the water and fertilizer conservation capacity of the soil, and has a remarkable effect on increasing the yield and income of crops.

The present application relates to the technical field of agricultural bio-fertilizer, and particularly relates to a special bio-organic fertilizer for sugar beet and a preparation method thereof, wherein sugar beet residues and sugar filter mud are mixed, humic acid, bentonite and amino acid fertilizer are added to obtain a pretreated substrate; aerobic fermentation is carried out, and the end point of aerobic fermentation is determined based on the carbon-nitrogen ratio and seed germination index to obtain a matured substrate; phosphorus-dissolving bacillus subtilis, bacillus mucilaginosus, azotobacter chroococcum, trichoderma harzianum and bacillus subtilis licheniformis are respectively expanded to target viable cell counts, and are mixed according to a mass ratio to obtain a composite functional microbial agent; the cooled matured substrate is inoculated into the composite functional microbial agent for anaerobic fermentation, and the end point of anaerobic fermentation is determined to obtain a functional microorganism colonization matured substrate; gamma-polyglutamic acid is dissolved in sterile water to prepare a gamma-polyglutamic acid spraying solution, zeolite powder and phosphate rock powder are added to obtain a granulation product; the granulation product is low-temperature dried to obtain the special bio-organic fertilizer for sugar beet. The present application effectively reduces the incidence of sugar beet root rot.

The application relates to the technical field of concrete, and particularly discloses a concrete containing recycled aggregate, which comprises the following components: water, cement, fly ash, fine sand, modified recycled aggregate, gravel and water reducing agent; and the modification method of the modified recycled aggregate comprises the following steps: 1) preparing a liquid culture medium and a modification liquid; 2) adding heat-loving streptococcus lactis, bacillus subtilis and azotobacter chroococcum to obtain a bacterial suspension; 3) putting the recycled aggregate into the bacterial suspension for soaking, filtering out the recycled aggregate to obtain pretreated recycled aggregate; and 4) culturing the pretreated recycled aggregate at a temperature of 25-30 DEG C and a relative humidity of 80-95% for 7-10 days, and then drying to obtain the modified recycled aggregate; the application has the advantages that the negative influence of the recycled aggregate on the strength of the concrete is reduced, the accumulated waste concrete solid can be better digested, the application is more friendly to the environment, and the environmental pollution can be better reduced.

The invention discloses a microbial agent for biologically preventing and treating rice diseases and application thereof, and belongs to the technical field of agricultural microorganisms, a preparation method comprises the following steps: respectively loading a trichoderma harzianum spore liquid, a bacillus spore liquid, a streptomyces jingyangensis mycelium fragment suspension and an azospirillum suspension on a charcoal carrier with a hierarchical pore structure; embedding with a coating solution containing rhodopseudomonas palustris spore solution to obtain a coating carrier; granulating and forming the coating carrier, humic acid powder, sodium carboxymethyl cellulose and water to obtain a microbial agent; the biochar carrier comprises a macroporous carbon carrier and a microporous carbon carrier; the pore diameter of the macroporous carbon carrier is 10-100 microns, and the macroporous carbon carrier is loaded with trichoderma harzianum spore liquid and streptomyces jingyangensis mycelium fragment suspension; the pore diameter of the microporous carbon carrier is less than 10 microns, and the microporous carbon carrier is loaded with bacillus spore liquid and azospirillum suspension; the three bottlenecks of flora mutual inhibition, insufficient slow release and degradation limitation are broken through, and an irreplaceable solution is provided for rice disease prevention and control and soil micro-ecological reconstruction.