18 results about "Azotobacter chroococcum" patented technology

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for heavy metal contaminated soil and a biological bacterial fertilizer and a preparation method thereof. The micro-ecological restoration liquid complex microbial inoculant suitable for the heavy metal contaminated soil comprises nitrogen-fixing bacteria: azotobacter chroococcum ACCC 11104, phosphate solubilizing bacteria: pseudomonas fluorescens ACCC 03031, potassium solubilizing bacteria: bacillus mucilaginosus CCTCC KB20082790, growth-promoting bacteria: azotobacter braziliensis CCTCC AB 91001, bacillus subtilis CGMCC 17161, synergistic bacteria: trichoderma asperellum CGMCC 3.17461, and restoration bacteria: pseudomonas putida CGMCC 1.4533. The biological bacterial fertilizer for the heavy metal contaminated soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the heavy metal contaminated soil is greatly improved, the heavy metal contaminated soil is effectively improved, and the crop yield is remarkably increased.

The invention belongs to the technical field of biological bacterial fertilizers, and particularly relates to a high-salt-tolerance microbial liquid complex microbial inoculant suitable for saline-alkali desertification land and a biological bacterial fertilizer thereof. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation of three stages: salt-tolerant repair bacteria Nocardia halophila, salt-tolerant functional bacteria Bacillus subtilis and Azospirillum brasilense, nitrogen-fixing functional bacteria Azotobacter chroococcum, phosphorus-solubilizing functional bacteria Pseudomonas fluorescens, and nitrogen-solubilizing functional bacteria Pseudomonas fluorescens. The bacillus mucilaginosus is used as a potassium-dissolving functional bacterium, the trichoderma asperellum is used as a biocontrol growth-promoting bacterium, and the trichoderma harzianum is used as a rhizosphere optimizing bacterium. The biological bacterial fertilizer is prepared from the liquid complex microbial inoculant. Through scientific compatibility of different strains provided by the invention, efficient improvement and crop yield increase of the saline-alkali desertification land are realized, and the strain has important economic and social values.

The invention discloses an oil shale waste residue microwave activation and microorganism coupling soil improvement method which comprises the following steps: crushing and screening oil shale dry distillation residues, and adding ground phosphate rock when necessary; then carrying out two-stage microwave activation treatment to obtain an activated waste residue carrier with high specific surface area and low content of harmful substances; mixing the activated carrier with a compound microbial agent (containing azotobacter chroococcum, silicate bacteria and cellulose degrading bacteria) and straws according to a specific ratio, and carrying out stack retting to prepare a harmless activated waste residue modifier; finally, the application amount is determined according to the soil type, and the fertilizer is applied to soil. According to the invention, harmlessness and high-value recycling of the oil shale waste residues are realized, and the prepared modifier can significantly increase the soil nutrient content, improve the soil structure, adjust the pH value and promote crop growth, is suitable for improvement of various degraded soil, has the advantages of efficient process, low cost, environmental friendliness and the like, and has a relatively high popularization value.

The invention relates to the technical field of biology, and discloses a recombinant N-acetylglucosamine-1-phosphate uridine transferase (GlmU) mutant and a preparation method thereof. The sequence of the recombinase is formed by connecting an N-acetylglucosamine-1-uridine phosphate transferase (GlmU) mutant (L113V-V172I) from Azotobacter chroococcum, an N-terminal histidine tag (His-tag) and a small ubiquitin-like modified protein tag (SUMO-tag) in series. The invention further discloses a preparation method of the recombinase. Compared with a recombinant wild type N-acetylglucosamine-1-uridine phosphate transferase with the same source, the recombinant N-acetylglucosamine-1-uridine phosphate transferase mutant has higher activity which is 1.5 times of that of the recombinant wild type and higher stability, and the residual activity of the mutant is 66.5% after the mutant is placed at room temperature for 5 days, so that the mutant has a good application prospect in the field of N-acetylglucosamine-1-uridine phosphate transferase. And the soluble expression quantity of the recombinant mutant N-acetylglucosamine-1-uridine phosphate transferase is increased by 5 times.

The invention provides a saline-alkali soil conditioner and a preparation method thereof. The preparation method comprises the following steps: S1, preparing polystyrene microspheres coated with polyethyleneimine; s2, preparing attapulgite of which the surface is grafted with TEMPO oxidized cellulose, and then carbonizing the attapulgite to obtain a carbon-coated attapulgite composite material; s3, preparing composite aerogel; s4, bacillus pasteurii and azotobacter chroococcum are loaded on the composite aerogel, and the saline-alkali soil conditioner is obtained. The double strains are loaded on the composite aerogel, controlled release and slow release of the strains are achieved, in addition, the two microbial agents serve as dominant flora, breeding of harmful pathogens in soil can be inhibited, soil micro-ecological balance can be adjusted, and a healthy rhizosphere environment is created for follow-up crop planting.

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for saline-alkali soil and a biological bacterial fertilizer of the micro-ecological restoration liquid composite microbial agent. The micro-ecological restoration liquid complex microbial inoculant suitable for the saline-alkali soil is prepared from restoration salt-tolerant bacteria, namely bacillus subtilis, nocardia halospora, nitrogen-fixing bacteria, namely azotobacter chroococcum, phosphate solubilizing bacteria, namely pseudomonas fluorescens, potassium solubilizing bacteria, namely bacillus mucilaginosus, growth-promoting bacteria, namely azospirillum brasilense, biocontrol bacteria, namely bacillus megatherium and synergistic bacteria, wherein the restoration salt-tolerant bacteria are bacillus subtilis, nocardia halospora, the nitrogen-fixing bacteria are azotobacter chroococcum, the phosphate solubilizing bacteria are pseudomonas fluorescens, the potassium solubilizing bacteria are bacillus mucilaginosus, the growth-promoting bacteria are azospirillum brasilense, the biocontrol bacteria are bacillus megatherium, and the synergistic bacteria are prepared from bacillus subtilis The biological bacterial fertilizer for the saline-alkali soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the saline-alkali soil is greatly improved, the saline-alkali soil is effectively improved, and the crop yield is remarkably increased.

The invention discloses Azospirillum brasilense capable of producing protease, decomposing potassium, degrading cellulose, promoting growth and relieving NaCl stress of plants and application of the Azospirillum brasilense. The invention provides nitrogen-fixing spirillum or a progeny thereof, the nitrogen-fixing spirillum is Dongying nitrogen-fixing spirillum, the strain number of the nitrogen-fixing spirillum is RS252, and the registration number of the nitrogen-fixing spirillum in the China General Microbiological Culture Collection Center is CGMCC (China General Microbiological Culture Collection Center) No. 31494. The strain is a new strain of azospirillum, and has the capabilities of producing protease, decomposing potassium and degrading cellulose. Pot experiments show that under the stress of NaCl, compared with an uninoculated negative control group, after the strain is inoculated, the root length and underground fresh weight of rice can be improved, and the strain can be used as a microbial organic fertilizer for improving soil fertility, relieving the NaCl stress of crops and improving the NaCl stress adaptability of the crops.

The present application discloses a strain of azotobacter chroococcum with the abilities of producing protease, decomposing potassium and cellulose, promoting growth and relieving plant NaCl stress, and application thereof. Azospirillum dongyingense The present application provides the azotobacter chroococcum or its offspring, the azotobacter chroococcum is azotobacter chroococcum (Azotobacter chroococcum) of Dongying, the strain number is RS252, and the registration number of the Center of General Microbiological Culture Collection of China Microbial Culture Collection Committee is CGMCC No. 31494. The strain is a new strain of azotobacter chroococcum genus, has the abilities of producing protease, decomposing potassium and cellulose. Potted test shows that compared with the negative control group without inoculation, the strain can improve the root length and underground fresh weight of rice under NaCl stress, and the strain can be used as microbial organic fertilizer to improve soil fertility, relieve crop NaCl stress and improve the adaptability of crops to NaCl stress.

The invention discloses a preparation method of a healthy and environment-friendly household lawn planting fertilizer, and belongs to the technical field of agricultural fertilizers. The invention relates to a preparation method of a healthy and environment-friendly household lawn planting fertilizer. The preparation method comprises the following steps: 1) efficiently converting kitchen garbage and garden waste by adopting a complex microbial inoculant (azotobacter chroococcum, thermophilic cellulose decomposition bacteria and candida tropicalis) in a specific ratio; 2) designing an intelligent response type core-shell coating structure, and realizing accurate controlled release of nutrients through the synergistic effect of a PHBV copolymer core layer, a modified starch interface layer and a temperature-sensitive nano composite shell layer; and 3) a safe combination of a bitter agent and a botanical insecticide is innovatively used. The product has the characteristics of quick start and long-acting supply, can obviously improve the soil environment, is completely biodegradable and pollution-free, and solves the problems of single function and serious pollution of the traditional fertilizer. The preparation process ensures stable product performance by optimizing fermentation parameters and an electrospray coating technology, and is particularly suitable for ecological maintenance requirements of family lawns.

The invention relates to the field of microbial agents, in particular to a universal soil micro-ecological restoration liquid composite microbial agent suitable for industrial production and a biological nitrogen fertilizer thereof. Comprising six functional strains: nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium-solubilizing bacteria, growth-promoting bacteria, biocontrol bacteria and synergistic bacteria, the nitrogen-fixing bacteria are azotobacter chroococcum, the phosphate-solubilizing bacteria are pseudomonas fluorescens, the potassium-solubilizing bacteria are bacillus mucilaginosus, the growth-promoting bacteria are azotobacter braziliensis, the biocontrol bacteria are bacillus subtilis, and the synergistic bacteria are trichoderma asperellum; the liquid complex microbial inoculant is prepared from the six functional strains through liquid fermentation by using electrochemical low-Hertz water. The biological nitrogen fertilizer is prepared by using the liquid complex microbial inoculant, exerts the functions of nitrogen fixation, phosphate solubilization, potassium solubilization, growth promotion, biocontrol and synergy, also can reduce the dependence on chemical fertilizers, not only can improve the soil quality, but also achieves the synergy goals of crop yield increase, quality improvement and soil health.

The invention discloses a method for preparing vegetation planting soil by utilizing industrial solid waste and microorganisms and application of the vegetation planting soil. The method comprises the following steps: mixing 80-90% of high titanium slag, 7-15% of sandy soil or phosphogypsum and 2-5% of titanium gypsum into a vegetation matrix according to specific particle size grading; inoculating a composite strain consisting of bacillus mucilaginosus and azotobacter chroococcum, fermenting at 30 DEG C for 12-18 days, and dynamically monitoring the contents of alkali-hydrolyzable nitrogen, available phosphorus and potassium and biologically extractable titanium; then, alfalfa is planted, arbuscular mycorrhizal fungi and rhizobium suspensions with the OD600 being 0.8-1.0 are regularly irrigated, and curing is conducted for 12-18 days; and irrigating and maintaining for 12-18 days by using chlorella propagation liquid every 4-6 days, and finally reserving the whole plant or harvesting to obtain the plant growing planting soil. According to the method, industrial solid waste is efficiently converted into a functional planting medium through the multi-stage biological-mineral synergistic effect, solid waste recycling and soil function reconstruction are achieved, and the method has the environment restoration and ecological reconstruction value.

The invention discloses a preparation method of a biogas slurry-based organic aqueous solution fertilizer, which comprises the following steps: filtering fresh anaerobic digestion biogas slurry, adding a dispersing agent, adjusting the pH value, mixing with a composite high-temperature bacterial agent, carrying out primary fermentation, carrying out secondary electrochemical treatment, adjusting the content ratio of nitrogen to phosphorus to potassium, adding a seaweed extract, a water-retaining agent and a preservative, and carrying out secondary electrochemical treatment to obtain the biogas slurry-based organic aqueous solution fertilizer. Uniformly mixing and curing to obtain an organic water solution fertilizer; wherein the composite high-temperature fungicide is prepared by compounding bacillus licheniformis, sporotrichum thermophile and azotobacter chroococcum according to the viable count ratio of 3: 1: 1. The high-end functional liquid fertilizer product with market competitiveness is prepared by efficiently treating biogas slurry through staged and multi-technology cooperation, decomposing organic matters and plant toxic substances and passivating metals.

The invention relates to the field of microbial agents, in particular to a soil micro-ecological restoration liquid composite microbial agent and biological bacterial fertilizer suitable for hillside dry land. The soil micro-ecological restoration liquid complex microbial inoculant suitable for the hillside dry land comprises nitrogen-fixing bacteria: azotobacter chroococcum ACCC 11104, phosphate solubilizing bacteria: pseudomonas fluorescens ACCC 03031, potassium solubilizing bacteria: bacillus mucilaginosus CCTCC KB20082790, growth-promoting bacteria: azospirillum brazil CCTCC AB 91001, bacillus subtilis CGMCC 17161, synergistic bacteria: trichoderma asperellum CGMCC 3.17461, and drought-resistant bacteria: bacillus megatherium ACCC 11099 and streptomyces CCTCC KA 2008039. The biological bacterial fertilizer for the hillside dry land is prepared from the liquid complex microbial inoculant, and compared with a conventional biological bacterial fertilizer, the biological bacterial fertilizer has the advantages that the ecological adaptability to the hillside dry land is greatly improved, the hillside dry land is effectively improved, and the crop yield is remarkably increased.

The invention relates to the technical field of organic fertilizer production, and particularly discloses a mink and fox manure microbial fermentation organic fertilizer and a preparation method thereof. Candida lipolytica, bacillus subtilis, lactobacillus plantarum, azotobacter chroococcum and saccharomyces cerevisiae are compounded according to the volume ratio of 3: 3: 2: 1: 1 to prepare a zymophyte solution, the manure of minks and foxes is mixed with oyster mushroom bran, chestnut shells or corn straw according to a specific proportion, fermentation is started at the temperature of 35 DEG C, and the total fermentation period is 20-30 days. According to the preparation method provided by the invention, the degradation efficiency of grease in feces of minks and foxes is remarkably improved, the nitrogen loss is reduced, the total nitrogen content of the obtained organic fertilizer is greater than or equal to 1.97%, the total phosphorus content is greater than or equal to 0.34%, the coliform group is less than or equal to 98.5 CFU / g, and the standards of NY 884-2012 and GB 8172-1987 are met.

The invention relates to a promoter, a carrier and a transformant for specific inducible expression of arginine secretions of a rice root system and application of the promoter, the carrier and the transformant. The promoter can promote the expression of a target gene in a host under the induction of arginine secretion of a rice root system, especially the expression in nitrogen-fixing pseudomonas stutzeri. The promoter, the vector and the transformant disclosed by the invention have wide application potential in the aspect of construction of an artificial efficient rhizosphere combined nitrogen fixation system.

The invention provides a soil biological conditioning agent for rapidly increasing the earthworm proliferation rate. The soil biological conditioning agent is prepared from a compound microbial agent, a functional enzyme preparation and a humic acid carrier containing calcium alginate, the compound microbial agent comprises bacillus amyloliquefaciens (Bacillus amyloliquefaciens), azotobacter chroococcum (Azotobacter chroococcum) and Saccharomyces cerevisiae (Saccharomyces cerevisiae), and the compound microbial agent is prepared from bacillus amyloliquefaciens (Bacillus amyloliquefaciens), azotobacter chroococcum (Azotobacter chroococcum) and Saccharomyces cerevisiae (Saccharomyces cerevisiae (Saccharomyces cerevisiae). The functional enzyme preparation comprises cellulase and chitinase. According to the conditioning agent disclosed by the invention, a ternary synergistic system consisting of the compound microbial agent, the functional enzyme preparation and the humic acid carrier containing calcium alginate is constructed, so that a natural soil ecological process can be simulated and enhanced, and whole-course efficient regulation and control on earthworm nutrition supply and environment improvement are realized.

The invention discloses an implementation method of a compound microbial agent in early field saline-alkali soil treatment, and belongs to the technical field of saline-alkali soil treatment. The method comprises the steps of preparation of a compound microbial agent, pretreatment of early field saline-alkali soil, application of the compound microbial agent and matched management measures. The compound microbial agent is prepared by mixing bacillus subtilis, bacillus licheniformis, bacillus megaterium, bacillus mucilaginosus and azotobacter chroococcum according to the volume ratio of 3: 2: 2: 2: 1. The early field saline-alkali soil pretreatment comprises deep ploughing and soil loosening and land leveling; the fungicide is applied in two times, the first time is spraying application 10-15 days before sowing, and the second time is root irrigation application in the crop seedling stage. Through the synergistic effect of various strains, the soil salinity and alkalinity can be effectively reduced, the soil nutrient effectiveness is improved, the soil structure is improved, the crop growth is promoted, the yield is increased, the cost is low, no pollution is caused, and good economic benefits and ecological benefits are achieved.

The application discloses an environment-friendly biological organic fertilizer for improving acidified soil and a preparation method thereof, and belongs to the technical field of organic fertilizer. The biological organic fertilizer is prepared from the following raw materials in parts by weight: 10-20 parts of composite gel material, 80-100 parts of mature poultry manure, 10-20 parts of microbial agent, 30-50 parts of modified waste tea leaf biochar, 10-15 parts of urea, 5-10 parts of humic acid, 1-5 parts of superphosphoric acid calcium, and 1-5 parts of sodium carboxymethyl cellulose; the microbial agent is prepared by mixing bacillus firmus, pseudomonas plecoglossicida and azotobacter chroococcum at a volume ratio of 1:1:1. The biological organic fertilizer can significantly improve the soil pH, increase the content of organic matter in the soil, has good buffering capacity and long-term effect, and can enhance the water and fertilizer conservation capacity of the soil, and has a remarkable effect on increasing the yield and income of crops.