37 results about "Bacillus laterosporus" patented technology

The invention relates to the technical field of soil remediation, and particularly discloses a soil remediation agent containing construction waste resourceful treatment substances as well as a preparation method and application of the soil remediation agent. The soil remediation agent disclosed by the invention is prepared from 1.5 to 2.5 parts of construction waste resourceful treatment substances, 0.1 to 0.3 part of mineral substances and 0.6 to 1.0 part of modified kaolin, the preparation method of the construction waste resourceful treatment substance comprises the following steps: performing magnetic separation and winnowing purification on construction waste, crushing, pyrolyzing, soaking in an activating solution, filtering, drying and grinding to obtain the construction waste resourceful treatment substance. A preparation method of the modified kaolin comprises the following steps: uniformly mixing kaolin, corn starch and water, adding compound bacteria, uniformly mixing, fermenting, drying and grinding, thereby obtaining the modified kaolin. The compound bacteria are prepared from bacillus subtilis and bacillus laterosporus. By utilizing the soil remediation agent provided by the invention, the content of available cadmium in soil can be effectively reduced, so that the production safety of plant planting is improved, and the yield of plants can be effectively increased.

The invention relates to a biological fermentation organic fertilizer based on crop residues and a fermentation method and application, and belongs to the technical field of organic fertilizer production.The fermentation method of the biological fermentation organic fertilizer based on the crop residues comprises the steps that firstly, the crop residues are pretreated to obtain a premixed material A and a premixed material B; mixing saccharomycetes, lactic acid bacteria, bacillus laterosporus, flavobacterium flagelloides, cellulase, xylanase and nattokinase to obtain an enzyme and bacterium mixture, composting the premixed material A and the premixed material B, and mixing with the enzyme and bacterium mixture for fermentation. According to the biological fermentation organic fertilizer based on the crop residues, the crop residues are pretreated through multiple means before fermentation, and a specific enzyme and bacterium mixing agent is mixed in the fermentation process to promote fermentation of the crop residues, so that after the organic fertilizer acts on soil, the content of organic matter in the soil can be increased, and the organic matter content in the soil can be increased; and heavy metals in the soil can be obviously reduced.

The invention discloses a method for carrying out soil treatment by using ardealite and a microbial agent, and aims to solve the problem that ardealite as an industrial by-product is difficult to recycle. According to the method, 5% of a microbial agent (one of trichoderma harzianum, arbuscular mycorrhizal fungi or bacillus laterosporus) and 5%-15% of biochar are applied to phosphogypsum in a combined mode, the nutrient content of a phosphogypsum matrix is remarkably increased, and meanwhile specific pollutants (such as soluble phosphorus, fluorine and heavy metal) are stabilized and solidified. Experimental results show that the technology can effectively regulate and control the pH value of the phosphogypsum matrix, obviously increase the diversity of effective microbial communities and improve the micro-ecological environment of the phosphogypsum matrix. The ardealite combined with the microbial agent and the biochar can be used as a plant growth matrix or a soil substitute material for ecological restoration of mines, has the characteristics of low cost and high efficiency, and provides a feasible way for harmlessness and soil utilization of the ardealite.

This invention discloses a *Bacillus laterosporus* strain for the stable production of nonribosomal cyclic lipolipeptide and its construction method. The M1A gene in the Bogorol biosynthetic gene cluster is knocked out using CRISPR-Cas9 technology to block the Bogorol pathway, which competes with Brevicidine for substrates. Subsequently, the pMCCas9-S48 plasmid, which integrates S8 and S41 sgRNAs and their homologous arms as repair templates, is used to simultaneously knock out S8 and S41 family peptidase genes, preventing the expression of extracellular degradation enzymes. This invention also provides the application of the above strain in the preparation of nonribosomal cyclic lipolipeptide of Brevicidine and a method for preparing the nonribosomal cyclic lipolipeptide. This strain, through reducing metabolic competition and preventing product degradation, exhibits rapid cell growth, stable production of Brevicidine, and significantly reduced degradation rate, resulting in a 66.13% increase in fermentation yield compared to the unmodified strain. This invention solves the problems of degradation and unstable yield in the production of Brevicidine, provides an effective technical approach for the industrial production of Brevicidine, and provides important technical support for the development and application of novel antimicrobial peptide drugs.

This invention relates to the field of microbial technology, specifically to a strain of *Bacillus laterosporus* and its applications. The *Bacillus laterosporus* provided by this invention (… Brevibacillus laterosporus HNBR05-1 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 25886. *Bacillus laterosporus* HNBR05-1 exhibits highly effective inhibition of plant pathogens, demonstrating dual efficacy against plant diseases such as brown spot and bacterial leaf spot, with high control efficacy. It shows promising application prospects in plant disease control and lays the foundation for the development of highly effective biocontrol agents.

The invention discloses a self-propagating microbial soil conditioning agent and a preparation process thereof, and relates to the technical field of soil treatment. The invention relates to a self-propagating microbial soil conditioning agent. Comprising the following components in parts by mass: 13 to 21 parts of Orson's bacteria, 11 to 22 parts of bacillus licheniformis, 10 to 15 parts of bacillus mucilaginosus, 20 to 23 parts of bacillus megatherium, 15 to 25 parts of bacillus thuringiensis, 15 to 20 parts of bacillus laterosporus, 12 to 25 parts of bacillus mucilaginosus and 240 to 365 parts of plant organic matters. The colonic microorganism soil conditioning fungicide prepared from the components can remarkably improve the soil structure, improve the soil fertility and promote crop growth. Meanwhile, due to the fact that microorganisms have the self-breeding capacity, beneficial microbial communities can be continuously provided for the soil, and the ecological balance of the soil is maintained. In addition, the microbial agent also has the advantages of preventing and treating soil diseases, reducing the use amount of chemical fertilizers and the like, and has important significance for promoting agricultural sustainable development.

The present application relates to the field of microorganisms, and specifically discloses a Bacillus spporidia HP‑2 and an application thereof; a Bacillus spporidia HP‑2, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCCNO.M20221789; a biological fertilizer comprising the following raw materials: soybean meal, potassium phosphate, diammonium phosphate, EDTA chelated magnesium, EDTA chelated calcium, EDTA chelated iron, zinc sulfate, humic acid, biological fiber, breathable filler, and Bacillus spporidia HP‑2 bacterial liquid; the Bacillus spporidia HP‑2 bacterial liquid is prepared from Bacillus spporidia HP‑2; Bacillus spporidia HP‑2 has the advantages of high temperature resistance and alkali resistance, and can increase crop yield after application.

This invention discloses a plasmid for gene overexpression in non-pattern *Bacillus laterosporus*. Its leader region contains a methyltransferase expression cassette derived from the *Bacillus laterosporus* i83 genome, enabling stable existence in specific hosts containing the RM system. Based on this, a PidR2 promoter is added to obtain the pMCPidR2 shuttle plasmid. The PidR2 promoter effectively drives target gene expression, the pWV01 replicon ensures plasmid replication in non-pattern hosts, and the chloramphenicol resistance gene is used for positive colony selection. The plasmid contains a TraJ element, allowing it to be introduced into non-pattern microbial hosts via conjugation transfer. This invention also provides a target gene expression strategy in non-pattern microorganisms, achieving high transformation efficiency and high strain yield through conjugation transfer transformation of donor bacteria, conjugation transfer helper bacteria, and recipient bacteria, resulting in stable expression of the target protein. This invention provides an effective vector for efficient and stable gene overexpression in specific non-pattern microorganisms, helping to solve the problem of difficult genetic manipulation of industrial production strains.

The invention discloses a fermentation method and application of bacillus laterosporus, and belongs to the technical field of microbial fermentation engineering and biological control. In order to solve the technical problems of unstable fermentation level, low spore formation rate and the like of bacillus laterosporus, the invention provides a high-density fermentation method of bacillus laterosporus, which comprises the following steps: adding a nutrition-signal composite inducer in stages in the middle and later periods of fermentation, and coupling pH / DO two-stage control, so that the spore formation rate is greater than or equal to 95%; besides, fermentation liquor of bacillus laterosporus and the bentonite-trehalose-sodium glutamate composite carrier are mixed and then subjected to low-temperature spray drying, the viable bacterium yield of the prepared microbial agent is larger than or equal to 95%, the viable bacterium rate is larger than or equal to 90% after the microbial agent is stored for 12 months at the temperature of 25 DEG C, and the microbial agent shows a remarkable effect in tomato root-knot nematode prevention and treatment of livestock and poultry diarrhea and has good application prospects. The compound can completely or partially replace chemical pesticides and antibiotics, and has a wide market prospect.

The invention provides a novel bacillus laterosporus (bacillus megatherium Z2) and an application method of the novel bacillus laterosporus in aquaculture. Through screening and gene improvement, the strain has the characteristics of high temperature resistance, high salt resistance and acid and alkali resistance, and can remarkably inhibit growth of aquatic pathogenic bacteria, improve water quality and promote healthy growth of aquaculture species. According to the application method, the strain with the concentration of 1 * 10 < 6 > CFU / L to 1 * 10 < 8 > CFU / L is added into a water body, diseases are effectively controlled, and the breeding yield and the aquatic product quality are improved. Experimental results show that the bacterial strain is obviously superior to the prior art in the aspects of bacteriostatic effect, water quality improvement, economic benefit and the like, and has the characteristics of environment friendliness, high efficiency and safety. A sustainable biological control scheme is provided for aquaculture, and the application prospect is wide.

This invention discloses a Bacillus laterosporus SCUT-BR-2 strain capable of producing collagenase and its application in the extraction of animal collagen / collagen peptides. Specifically, the method includes: screening collagenase-producing strains from meat products, and extracting collagen from animal skin using a crude extract of the strain's collagenase through morphological observation, physiological and biochemical tests, and determination; simultaneously, using this crude extract to decompose bovine bone collagen to obtain a bovine bone collagen peptide with good antioxidant properties and anti-wrinkle, firming, soothing, and moisturizing effects.

The invention provides a special fertilizer used before fruit bearing of vine hanging watermelons as well as a preparation method and application of the special fertilizer, and belongs to the technical field of fertilizers. The special fertilizer comprises the following raw materials in parts by weight: 10-15 parts of macroelements, 0.07-0.09 part of microelements, 3-8 parts of a root growth promoting agent, 20-30 parts of potassium humate, 10-15 parts of a microbial agent and 2-5 parts of auxiliary materials, the root growth promoting agent is prepared from high-molecular polyglutamic acid, amino-oligosaccharide, nicotinamide and brassin; the microbial agent is prepared from bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis and azotobacter chroococcum. The special fertilizer provided by the invention can improve the absorption rate of nutrient substances, improve the root activity of watermelons, promote the growth of the root systems, stems and leaves of the watermelons, contribute to improving the absorption and utilization of nutrient components after fruit bearing in the later period, and lay a solid foundation for the growth of fruits in the later period.

The invention provides a trichoderma inoculant, a preparation method thereof and application of the trichoderma inoculant in improving yield and quality of beet, and belongs to the technical field of agricultural microbial inoculants. The trichoderma agent comprises the following components: trichoderma harzianum, trichoderma viride, purpureocillium lilacinum, bacillus amyloliquefaciens and bacillus laterosporus in a mass ratio of (3-5): (1-3): (2-4): (2-4): (1-2). According to the invention, the strains cooperate with each other to promote the growth of beet, effectively enhance the immune resistance of beet, and effectively prevent and reduce beet root rot, brown blotch and other diseases caused by continuous cropping; the photosynthesis efficiency of the beet can be enhanced, the accumulation of photosynthetic products is improved, the soil structure is improved, the number of beneficial bacteria groups in the soil is increased, and the rhizosphere environment is improved, so that the sugar content and yield of the beet are increased. By applying the microbial agent disclosed by the invention, multiple effects of increasing the yield per mu, increasing the sugar content of beet, improving soil, improving the fertilizer efficiency, resisting diseases, preventing insects and the like can be achieved, and farmers are helped to increase both production and income.

The application discloses a preparation method of an extractive for preventing and treating citrus anthracnose, and comprises the following steps: drying garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed, and then crushing the dried garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed; adding the crushed garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed into boiling water and stirring until uniform; continuing to cook; freezing; thawing after the freezing is completed; obtaining a freeze-thawed material; adding sodium dihydrogen phosphate into the freeze-thawed material; placing the freeze-thawed material in a fermentation tank; inoculating bacillus subtilis and bacillus mycoides for aerobic fermentation; inoculating bacillus licheniformis, bacillus laterosporus and bacillus thuringiensis for aerobic fermentation; completing the aerobic fermentation; carrying out anaerobic fermentation on the fermented material; stirring the fermented material and pure water until uniform; filtering after standing; taking filter residue and precipitate as extractive A; adjusting the PH value of filtrate to obtain extractive B; adding bagasse powder into the extractive A, compacting, packaging and bagging; and sealing and storing the extractive B. The extractive prepared by the application can efficiently prevent and treat citrus anthracnose, and has the characteristics of low production cost and no pollution to citrus planting environment.

The invention relates to the technical field of planting, in particular to a fungicide and fertilizer suitable for planting landscape plants. The microbial inoculum disclosed by the invention is prepared from the following components in parts by mass: 5 to 10 parts of bacillus coagulans microbial inoculum, 4 to 8 parts of bacillus laterosporus microbial inoculum, 10 to 18 parts of bacillus mucilaginosus microbial inoculum, 6 to 10 parts of bacillus velezensis microbial inoculum, 2 to 6 parts of trichoderma viride, 6 to 10 parts of bacillus amyloliquefaciens microbial inoculum and 2 to 6 parts of paecilomyces lilacinus. Through compatibility of various microbial agents, the soil structure can be improved, and the soil fertility can be improved. The microbial agent disclosed by the invention is prepared into a fertilizer, and the obtained fertilizer also has the effects of regulating the soil microhabitat, improving the soil structure and improving the soil fertility. The growth of garden plant roots can be promoted, and the growth performance of garden plants is improved. And a basis is provided for planting of landscape plants.

The invention discloses an agricultural microbial agent as well as a preparation method and application thereof. The agricultural microbial agent is prepared from bacillus laterosporus, bacillus subtilis and a vine-corn straw composite carrier used for loading the bacillus laterosporus and the bacillus subtilis. The preparation method comprises the following steps: step 1, culturing bacillus laterosporus and bacillus subtilis; step 2, preparing a microbial inoculum composite carrier; the application refers to application in straw degradation. According to the prepared microbial agent carrier, bacillus laterosporus and bacillus subtilis can be better loaded on the surface of the microbial agent carrier, and the preservation period of the microbial agent is longer, so that the prepared agricultural microbial agent has relatively lasting straw degradation capacity, meanwhile, the agricultural straw composting period can be remarkably shortened, and nitrogen loss is reduced.

The present invention provides a compound probiotic fertilizer for regulating the growth of Platycodon grandiflorum and its application, belonging to the field of microbial fertilizers. The compound probiotic fertilizer is prepared from a microbial inoculant JG-002, Platycodon grandiflorum root exudates, and a carrier. The mass ratio of the microbial inoculant JG-002, Platycodon grandiflorum root exudates, and the carrier is 100:1-5:10-20. The microbial inoculant JG-002 is composed of Bacillus subtilis, Bacillus licheniformis, and Bacillus laterosporus in a viable bacteria number ratio of 5:3:2. The carrier is humic acid, sodium alginate, and calcium chloride, and the mass ratio of humic acid, sodium alginate, and calcium chloride is 3-5:2-3:1-2. The compound probiotic fertilizer in the present invention has a biological antagonistic effect, can effectively inhibit the growth and reproduction of pathogenic microorganisms such as Fusarium in the soil, reduce the incidence of Platycodon grandiflorum root rot, and solve the continuous cropping obstacle.

The invention belongs to the field of rubber tree cultivation, and particularly relates to a biocontrol bacterium preparation for preventing and treating anthracnose of rubber trees and preparation of the biocontrol bacterium preparation. The biocontrol microbial agent is prepared from the following components in parts by weight: 0.5 to 1 part of bacillus velezensis HN-2, 1 to 1.5 parts of bacillus laterosporus, 5 to 10 parts of mango polysaccharide, 20 to 40 parts of herba king grass, 10 to 20 parts of clausena lansium peel, 15 to 25 parts of pitaya stem and 8 to 12 parts of rubber leaf. According to the biocontrol microbial inoculum, waste pitaya stems in the pitaya cultivation process, waste rubber leaves in the rubber tree cultivation process and wampee peel are used for preparing an extracting solution for strain fermentation, and the biocontrol microbial inoculum has good inhibitory activity on colletotrichum gloeosporioides of the rubber trees and has good application prospects in the aspect of preventing and treating the colletotrichum gloeosporioides of the rubber trees.

The invention discloses a functional composite microbial inoculant for ecological improvement of moderate and severe saline-alkali soil and a preparation method and application thereof. The composite bacterial agent is prepared by compounding bacterial solutions of paenibacillus polymyxa SMr02, bacillus velezensis DS-R-B-3-5, bacillus laterosporus BHr6-5, lysinibacillus fusiformis SCB19 and pseudomonas japonicus D109 according to the volume ratio of 3: 2: 2: 2: 1. The complex microbial inoculant disclosed by the invention has good saline-alkali tolerance, can obviously improve the stable soil fertility and nutrient content in moderate and severe saline-alkali soil, reduce the soil salinity and alkalinity, relieve saline-alkali stress of salt-tolerant crops and promote growth of the salt-tolerant crops, is simple and convenient to operate, low in cost and environment-friendly, and has a wide application prospect. The method is suitable for ecological improvement of moderate and severe saline-alkali soil in arid areas, irrigation areas in the middle and upstream of the Yellow River and the like, and has good ecological benefits and economic benefits.

The invention belongs to the technical field of agricultural biology, and particularly discloses a functional seedling culture substrate for preventing and treating root-knot nematode and application of the functional seedling culture substrate. The functional seedling raising substrate is composed of a solid seedling raising substrate and a compound microbial agent uniformly dispersed in the solid seedling raising substrate. The compound microbial agent is prepared by carrying out mixed fermentation on bacillus velezensis RKN1111, bacillus laterosporus CDD-01 and paenibacillus mucilaginosus HX-02. The invention further discloses a preparation method of the compound microbial agent. When the functional seedling culture substrate is used for seedling culture, the colonization rate of the compound microbial agent in seedling rhizosphere is greatly improved, the inside and the outside of root lumps of cultured germchit are rich in active biocontrol bacteria during transplanting, the germchit can continuously play a high-efficiency and stable prevention and control role after transplanting, and the growth of the germchit is remarkably promoted. According to the method, the prevention and control opportunity of the root-knot nematode is greatly advanced from a field period to a controllable seedling raising period, active immunization is achieved, and the technical problems that colonization is difficult and the effect is unstable when a biocontrol microbial inoculum is applied to traditional root irrigation or fields are effectively solved.

The invention relates to the technical field of feed additives, and particularly discloses a compound feed additive as well as a preparation method and application thereof. The compound feed additive provided by the invention comprises the following components in parts by weight: 1-8 parts of a saw palmetto extract, 1-5 parts of an inonotus obliquus extract, 0.5-2.5 parts of a rhizoma dioscoreae nipponicae extract, 20-30 parts of a raspberry extract, 3-5 parts of a cortex erythrinae extract, 3-5 parts of compound bacteria and 10-30 parts of a carrier, furthermore, the compound bacteria are selected from enterococcus faecium, bacillus laterosporus, acetobacter ollani, lactobacillus acidophilus and lactobacillus rhamnosus; the invention also provides an animal feed prepared from the compound feed additive. The compound feed additive provided by the invention can improve the growth performance of animals from multiple aspects of enhancing animal immunity, improving animal intestinal microbe balance, increasing the feed conversion rate and the like, so that the breeding benefits of the livestock and poultry breeding industry are improved.

The invention relates to the technical field of microorganisms, and particularly discloses bacillus laterosporus, a fungicide, a preparation method and application of the bacillus laterosporus to biological prevention and control of ticks. The bacillus laterosporus is brevibacillus laterosporus HNYJTHS227, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.31258, the preservation date is July 10, 2024, the preservation unit is China General Microbiological Culture Collection Center, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention further provides a bacillus laterosporus biological agent, the biological agent is simple in preparation method, can achieve a good tick killing effect by being sprayed on animal body surfaces, and is convenient to use, free of residues, efficient and safe.

The invention relates to the technical field of prevention and control of melanogaster. The invention provides a biocontrol bacterium composition and application of the biocontrol bacterium composition in prevention and treatment of melanogaster. The biocontrol bacterium composition comprises the following raw materials in parts by weight: 45-55 parts of a metarhizium anisopliae bacterium agent, 15-25 parts of a bacillus thuringiensis bacterium agent, 10-15 parts of a bacillus licheniformis bacterium agent and 10-15 parts of a bacillus laterosporus bacterium agent. The biocontrol bacterium composition disclosed by the invention contains a plurality of different strains, and the different strains are matched with one another, so that the melanogaster can be effectively prevented and treated, and the killing efficiency of the product is improved. And the used strain is environment-friendly, the efficiency is higher after the strain is loaded by a carrier, and the strain is more convenient to use.

The application relates to the technical field of feed additives, and particularly discloses a compound feed additive as well as a preparation method and application thereof. The compound feed additive provided by the application comprises the following components in parts by weight: saw leaf palm extract 1-8 parts, white birch mushroom extract 1-5 parts, Rhizoma Anemarrhenae extract 0.5-2.5 parts, raspberry extract 20-30 parts, sea-tortoise skin extract 3-5 parts, compound bacteria 3-5 parts and carrier 10-30 parts; further, the compound bacteria are selected from Enterococcus faecium, Bacillus laterosporus, Acetobacter orleanensis, Lactobacillus acidophilus and Lactobacillus rhamnosus; and the application also provides animal feed prepared from the compound feed additive. The compound feed additive provided by the application can improve the growth performance of animals from aspects of enhancing the immunity of animals, improving the intestinal microorganism balance of animals, improving the feed conversion rate and the like, so as to improve the breeding benefit of livestock and poultry breeding.

The invention discloses a fermented feed for increasing the content of astaxanthin in whole shrimps and muscles of penaeus vannamei as well as a production process and application of the fermented feed. The fermented feed is prepared by mixed fermentation of a fermentation substrate, a nutrient medium and strains, wherein the strain is prepared from the following raw materials in percentage by mass based on the total weight of all the raw materials: 0.5%-2% of bacillus subtilis, 0.5%-2% of bacillus laterosporus, 1%-3% of saccharomyces cerevisiae, 3%-5% of phaffia rhodozyma, 2%-3% of rhodopseudomonas palustris, 0.5%-2% of lactobacillus acidophilus and 0.5%-2% of enterococcus faecium. The fermented feed is used for feeding the penaeus vannamei, so that the growth indexes of penaeus vannamei bodies can be effectively improved, the number of culturable bacteria and vibrios in aquaculture water is increased, the content of astaxanthin in the whole penaeus vannamei and muscles and the body color of the penaeus vannamei bodies are increased, the digestion and absorption of the astaxanthin of the penaeus vannamei are improved, and the survival rate of the penaeus vannamei is increased. Furthermore, the nutritional value of the penaeus vannamei boone is improved.

The application relates to the technical field of soil remediation, and specifically discloses a soil remediation agent containing construction waste resource processing products and a preparation method and application thereof. The soil remediation agent disclosed by the application comprises 1.5-2.5 parts of construction waste resource processing products, 0.1-0.3 parts of mineral substances and 0.6-1.0 parts of modified kaolin. The preparation method of the construction waste resource processing products is as follows: the construction waste is purified through magnetic separation and air separation, crushed, pyrolyzed, soaked in an activation liquid, filtered, dried and ground to obtain the construction waste resource processing products; the preparation method of the modified kaolin is as follows: kaolin, corn starch and water are uniformly mixed, compound bacteria are added and uniformly mixed, and then fermentation, drying and grinding are carried out to obtain the modified kaolin; the compound bacteria are composed of bacillus subtilis and bacillus laterosporus. The soil remediation agent provided by the application can effectively reduce the content of effective cadmium in soil, thereby improving the production safety of plant planting and effectively improving the yield of plants.

The application discloses a planting method for improving corn yield and belongs to the technical field of corn planting. The planting method comprises the following steps: applying a compound microbial fertilizer as base fertilizer before sowing and additionally applying the compound microbial fertilizer during the corn growth period; the compound microbial fertilizer contains the following components in parts by weight: 38 parts of compound microbial bacteria, 42 parts of rotten cow dung, 12 parts of corn straw powder, 20 parts of wood ash, 7 parts of humic acid and 4 parts of potassium sulfate. The compound microbial bacteria comprise bacillus subtilis, bacillus licheniformis and bacillus laterosporus, and the ratio of the three is 0.8:1:1.2. The application improves the soil structure by using the biological microbial fertilizer, increases the soil organic matter content, improves the soil aggregate structure, makes the corn grow more robust, has more large grains and increases the thousand-grain weight, and thus significantly improves the corn yield and quality.

The invention belongs to the field of cultivation of jack fruits, and particularly relates to a plant immunomodulator for preventing cracking of jack fruits and a use method of the plant immunomodulator. According to the plant immunomodulator disclosed by the invention, eucalyptus leaves, fragrant eupatorium herb, wedelia chinensis and pitaya stems are used as fermentation materials, bacillus licheniformis, bacillus laterosporus, trichoderma harzianum and metarhizium anisopliae are respectively added for step-by-step fermentation treatment, and the prepared plant immunomodulator can be used for remarkably reducing the fruit cracking rate of jackfruits and improving the commercial fruit rate and yield; moreover, the pulp rate of the jackfruits can be increased to a certain extent, the effect of double improvement of the yield and the fruit quality of the jackfruits is achieved, and a new way is provided for improvement of planting economic benefits of the jackfruits.

This invention discloses a multifunctional stem-coating medicated fertilizer solution, its preparation method, and its application, belonging to the field of bio-organic fertilizer technology. The multifunctional stem-coating medicated fertilizer solution of this invention comprises the following raw materials: wood vinegar, microbial inoculants, earthworm protein peptides, *Alternaria solani* extract, potassium humate, lecithin, sodium carboxymethyl cellulose, chitin, urea, potassium dihydrogen phosphate, a chelated trace element mixture, and water. The microbial inoculants include *Bacillus laterosporus*, *Burkholderia ulmoides*, and *Trichoderma pleurotus*. This invention, through the scientific compounding of three specific strains, works synergistically with the other raw materials in the formula, solving the problems of limited disease resistance and low fertilizer utilization rates in existing apple tree stem-coating agents. While effectively controlling apple tree rot, it also significantly promotes fruit enlargement, increases yield and quality, improves tree vigor, and is environmentally friendly, with no adverse effects on the environment or fruit, showing broad application prospects.

The present application relates to the field of biocontrol agents, and specifically discloses a microbial preparation containing Bacillus laterosporus and a preparation method thereof. A preparation method of a microbial preparation containing Bacillus laterosporus comprises the following steps: adding the clay to a liquid culture medium after sterilization, then adding Bacillus laterosporus, mixing evenly, maturing, centrifuging, washing, adding ultrapure water, and preparing a modified clay suspension; by weight, 30-50 parts of the modified clay suspension are mixed with 10-15 parts of concentrated biogas slurry, 1-2 parts of glycerol, 2-4 parts of γ-aminobutyric acid, and 3-7 parts of selenium nanomaterials to prepare a microbial agent. The microbial preparation of the present application has the advantages of promoting seed germination and crop growth.