13 results about "Bacillus laterosporus" patented technology

This invention discloses a *Bacillus laterosporus* strain for the stable production of nonribosomal cyclic lipolipeptide and its construction method. The M1A gene in the Bogorol biosynthetic gene cluster is knocked out using CRISPR-Cas9 technology to block the Bogorol pathway, which competes with Brevicidine for substrates. Subsequently, the pMCCas9-S48 plasmid, which integrates S8 and S41 sgRNAs and their homologous arms as repair templates, is used to simultaneously knock out S8 and S41 family peptidase genes, preventing the expression of extracellular degradation enzymes. This invention also provides the application of the above strain in the preparation of nonribosomal cyclic lipolipeptide of Brevicidine and a method for preparing the nonribosomal cyclic lipolipeptide. This strain, through reducing metabolic competition and preventing product degradation, exhibits rapid cell growth, stable production of Brevicidine, and significantly reduced degradation rate, resulting in a 66.13% increase in fermentation yield compared to the unmodified strain. This invention solves the problems of degradation and unstable yield in the production of Brevicidine, provides an effective technical approach for the industrial production of Brevicidine, and provides important technical support for the development and application of novel antimicrobial peptide drugs.

This invention relates to the field of microbial technology, specifically to a strain of *Bacillus laterosporus* and its applications. The *Bacillus laterosporus* provided by this invention (… Brevibacillus laterosporus HNBR05-1 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 25886. *Bacillus laterosporus* HNBR05-1 exhibits highly effective inhibition of plant pathogens, demonstrating dual efficacy against plant diseases such as brown spot and bacterial leaf spot, with high control efficacy. It shows promising application prospects in plant disease control and lays the foundation for the development of highly effective biocontrol agents.

This invention discloses a plasmid for gene overexpression in non-pattern *Bacillus laterosporus*. Its leader region contains a methyltransferase expression cassette derived from the *Bacillus laterosporus* i83 genome, enabling stable existence in specific hosts containing the RM system. Based on this, a PidR2 promoter is added to obtain the pMCPidR2 shuttle plasmid. The PidR2 promoter effectively drives target gene expression, the pWV01 replicon ensures plasmid replication in non-pattern hosts, and the chloramphenicol resistance gene is used for positive colony selection. The plasmid contains a TraJ element, allowing it to be introduced into non-pattern microbial hosts via conjugation transfer. This invention also provides a target gene expression strategy in non-pattern microorganisms, achieving high transformation efficiency and high strain yield through conjugation transfer transformation of donor bacteria, conjugation transfer helper bacteria, and recipient bacteria, resulting in stable expression of the target protein. This invention provides an effective vector for efficient and stable gene overexpression in specific non-pattern microorganisms, helping to solve the problem of difficult genetic manipulation of industrial production strains.

The invention discloses a fermentation method and application of bacillus laterosporus, and belongs to the technical field of microbial fermentation engineering and biological control. In order to solve the technical problems of unstable fermentation level, low spore formation rate and the like of bacillus laterosporus, the invention provides a high-density fermentation method of bacillus laterosporus, which comprises the following steps: adding a nutrition-signal composite inducer in stages in the middle and later periods of fermentation, and coupling pH / DO two-stage control, so that the spore formation rate is greater than or equal to 95%; besides, fermentation liquor of bacillus laterosporus and the bentonite-trehalose-sodium glutamate composite carrier are mixed and then subjected to low-temperature spray drying, the viable bacterium yield of the prepared microbial agent is larger than or equal to 95%, the viable bacterium rate is larger than or equal to 90% after the microbial agent is stored for 12 months at the temperature of 25 DEG C, and the microbial agent shows a remarkable effect in tomato root-knot nematode prevention and treatment of livestock and poultry diarrhea and has good application prospects. The compound can completely or partially replace chemical pesticides and antibiotics, and has a wide market prospect.

The invention discloses an agricultural microbial agent as well as a preparation method and application thereof. The agricultural microbial agent is prepared from bacillus laterosporus, bacillus subtilis and a vine-corn straw composite carrier used for loading the bacillus laterosporus and the bacillus subtilis. The preparation method comprises the following steps: step 1, culturing bacillus laterosporus and bacillus subtilis; step 2, preparing a microbial inoculum composite carrier; the application refers to application in straw degradation. According to the prepared microbial agent carrier, bacillus laterosporus and bacillus subtilis can be better loaded on the surface of the microbial agent carrier, and the preservation period of the microbial agent is longer, so that the prepared agricultural microbial agent has relatively lasting straw degradation capacity, meanwhile, the agricultural straw composting period can be remarkably shortened, and nitrogen loss is reduced.

The invention discloses a functional composite microbial inoculant for ecological improvement of moderate and severe saline-alkali soil and a preparation method and application thereof. The composite bacterial agent is prepared by compounding bacterial solutions of paenibacillus polymyxa SMr02, bacillus velezensis DS-R-B-3-5, bacillus laterosporus BHr6-5, lysinibacillus fusiformis SCB19 and pseudomonas japonicus D109 according to the volume ratio of 3: 2: 2: 2: 1. The complex microbial inoculant disclosed by the invention has good saline-alkali tolerance, can obviously improve the stable soil fertility and nutrient content in moderate and severe saline-alkali soil, reduce the soil salinity and alkalinity, relieve saline-alkali stress of salt-tolerant crops and promote growth of the salt-tolerant crops, is simple and convenient to operate, low in cost and environment-friendly, and has a wide application prospect. The method is suitable for ecological improvement of moderate and severe saline-alkali soil in arid areas, irrigation areas in the middle and upstream of the Yellow River and the like, and has good ecological benefits and economic benefits.

The invention belongs to the technical field of agricultural biology, and particularly discloses a functional seedling culture substrate for preventing and treating root-knot nematode and application of the functional seedling culture substrate. The functional seedling raising substrate is composed of a solid seedling raising substrate and a compound microbial agent uniformly dispersed in the solid seedling raising substrate. The compound microbial agent is prepared by carrying out mixed fermentation on bacillus velezensis RKN1111, bacillus laterosporus CDD-01 and paenibacillus mucilaginosus HX-02. The invention further discloses a preparation method of the compound microbial agent. When the functional seedling culture substrate is used for seedling culture, the colonization rate of the compound microbial agent in seedling rhizosphere is greatly improved, the inside and the outside of root lumps of cultured germchit are rich in active biocontrol bacteria during transplanting, the germchit can continuously play a high-efficiency and stable prevention and control role after transplanting, and the growth of the germchit is remarkably promoted. According to the method, the prevention and control opportunity of the root-knot nematode is greatly advanced from a field period to a controllable seedling raising period, active immunization is achieved, and the technical problems that colonization is difficult and the effect is unstable when a biocontrol microbial inoculum is applied to traditional root irrigation or fields are effectively solved.

The application discloses a planting method for improving corn yield and belongs to the technical field of corn planting. The planting method comprises the following steps: applying a compound microbial fertilizer as base fertilizer before sowing and additionally applying the compound microbial fertilizer during the corn growth period; the compound microbial fertilizer contains the following components in parts by weight: 38 parts of compound microbial bacteria, 42 parts of rotten cow dung, 12 parts of corn straw powder, 20 parts of wood ash, 7 parts of humic acid and 4 parts of potassium sulfate. The compound microbial bacteria comprise bacillus subtilis, bacillus licheniformis and bacillus laterosporus, and the ratio of the three is 0.8:1:1.2. The application improves the soil structure by using the biological microbial fertilizer, increases the soil organic matter content, improves the soil aggregate structure, makes the corn grow more robust, has more large grains and increases the thousand-grain weight, and thus significantly improves the corn yield and quality.

The invention belongs to the field of cultivation of jack fruits, and particularly relates to a plant immunomodulator for preventing cracking of jack fruits and a use method of the plant immunomodulator. According to the plant immunomodulator disclosed by the invention, eucalyptus leaves, fragrant eupatorium herb, wedelia chinensis and pitaya stems are used as fermentation materials, bacillus licheniformis, bacillus laterosporus, trichoderma harzianum and metarhizium anisopliae are respectively added for step-by-step fermentation treatment, and the prepared plant immunomodulator can be used for remarkably reducing the fruit cracking rate of jackfruits and improving the commercial fruit rate and yield; moreover, the pulp rate of the jackfruits can be increased to a certain extent, the effect of double improvement of the yield and the fruit quality of the jackfruits is achieved, and a new way is provided for improvement of planting economic benefits of the jackfruits.

This invention discloses a multifunctional stem-coating medicated fertilizer solution, its preparation method, and its application, belonging to the field of bio-organic fertilizer technology. The multifunctional stem-coating medicated fertilizer solution of this invention comprises the following raw materials: wood vinegar, microbial inoculants, earthworm protein peptides, *Alternaria solani* extract, potassium humate, lecithin, sodium carboxymethyl cellulose, chitin, urea, potassium dihydrogen phosphate, a chelated trace element mixture, and water. The microbial inoculants include *Bacillus laterosporus*, *Burkholderia ulmoides*, and *Trichoderma pleurotus*. This invention, through the scientific compounding of three specific strains, works synergistically with the other raw materials in the formula, solving the problems of limited disease resistance and low fertilizer utilization rates in existing apple tree stem-coating agents. While effectively controlling apple tree rot, it also significantly promotes fruit enlargement, increases yield and quality, improves tree vigor, and is environmentally friendly, with no adverse effects on the environment or fruit, showing broad application prospects.

The invention belongs to the technical field of microbial remediation, and particularly relates to brevibacillus laterosporus M2H06 and application of the brevibacillus laterosporus M2H06 in degradation of zearalenone. The invention provides brevibacillus laterosporus for degrading zearalenone, the Latin name of the brevibacillus laterosporus is Brevibacillus laterosporus, the brevibacillus laterosporus is preserved in Guangdong Microbial Culture Collection Center, the preservation date is October 27, 2025, the preservation address is Guangdong, China, and the preservation number is GDMCC.No 67167. The brevibacillus laterosporus can be used for degrading zearalenone. And brevibacillus laterosporus M2H06. The brevibacillus laterosporus M2H06 provided by the invention can achieve a degradation rate of 99.25% for 5 [mu] g / mL zearalenone within 12 h, and is efficient, rapid and free of extra pollution. After the zearalenone is removed from the feed, the zearalenone does not need to be removed again.

This invention discloses a method for improving acidic tobacco-growing soil using a combination of biochar and microbial inoculants, belonging to the field of tobacco soil improvement technology. Addressing the problem of unstable effects of using biochar and microbial inoculants alone to improve acidic tobacco-growing soil in existing technologies, this invention applies biochar (450-1350 kg / hm²) and microbial inoculants (total application rate 30 kg / hm²) to acidic tobacco-growing soil. The microbial inoculants are applied twice: once as a basal application and once as a top dressing. Preferably, the biochar is prepared by carbonizing peanut shells at 400℃ under low-oxygen conditions for 20 minutes, with an application rate of 900 kg / hm². The microbial inoculant is a compound inoculant containing four bacteria, including Bacillus laterosporus, with half applied as a basal application and half as a top dressing. The top dressing is applied 15 days after transplanting, diluted 100 times, and used for root irrigation. This invention can significantly increase soil pH, improve soil fertility, promote tobacco growth, and improve yield and quality.

The invention provides a low-temperature-resistant complex microbial inoculant and application thereof. Specifically, the invention provides a low-temperature-resistant complex microbial inoculant which comprises bacillus licheniformis, bacillus megaterium and bacillus laterosporus. The invention also provides an application of the low-temperature-resistant complex microbial inoculant and a method for producing a liquid bacterial fertilizer by using the low-temperature-resistant complex microbial inoculant and using livestock urine as a nutrient source, and relates to the field of microorganisms. In a low-temperature environment, the complex microbial inoculant decomposes and converts livestock urine into a nutrient-rich and safe liquid bacterial fertilizer, the content of available nitrogen in the urine is increased (greater than or equal to 2.0 g / L), and the complex microbial inoculant has obvious ecological benefits and economic benefits when being applied to livestock manure treatment and agricultural production in northeast China.