Preparation method and application of extract for preventing and treating citrus anthracnose

Abstract

The application discloses a preparation method of an extractive for preventing and treating citrus anthracnose, and comprises the following steps: drying garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed, and then crushing the dried garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed; adding the crushed garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed into boiling water and stirring until uniform; continuing to cook; freezing; thawing after the freezing is completed; obtaining a freeze-thawed material; adding sodium dihydrogen phosphate into the freeze-thawed material; placing the freeze-thawed material in a fermentation tank; inoculating bacillus subtilis and bacillus mycoides for aerobic fermentation; inoculating bacillus licheniformis, bacillus laterosporus and bacillus thuringiensis for aerobic fermentation; completing the aerobic fermentation; carrying out anaerobic fermentation on the fermented material; stirring the fermented material and pure water until uniform; filtering after standing; taking filter residue and precipitate as extractive A; adjusting the PH value of filtrate to obtain extractive B; adding bagasse powder into the extractive A, compacting, packaging and bagging; and sealing and storing the extractive B. The extractive prepared by the application can efficiently prevent and treat citrus anthracnose, and has the characteristics of low production cost and no pollution to citrus planting environment.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of citrus disease prevention and control. More specifically, the present application relates to a preparation method and application of an extract for preventing and controlling citrus anthracnose. BACKGROUND

[0002] Citrus prefers warm and humid climate, and is mainly distributed in Yunnan, Guangxi, Guangdong, southern Sichuan, southern Hunan, southern Jiangxi and part of Fujian, and is one of the important economic crops in China. Due to the continuous planting of citrus, the diseases on citrus trees have also increased year by year, and "anthracnose" is one of the main hazards of citrus. The pathogenic bacteria causing citrus anthracnose are the glomerella of the deuteromycotina order, and the sexual stage is the small cluster shell of the ascomycota order. The host range of the pathogen is wide, and it occurs in all major citrus planting areas in China, seriously damaging production, transportation and storage. Anthracnose mainly damages the aboveground part of the tree, causing leaf fall, fruit drop, branch dryness, etc., and in severe cases, it can cause tree decline, yield reduction, and even tree death.

[0003] At present, the main technical means for preventing and controlling citrus anthracnose is to use methylthiophanate wettable powder, carbendazim wettable powder, chlorothalonil water agent, benzene ether azole water dispersible granules, and some pesticides with certain toxicity such as prochloraz for prevention and control. Pesticide pollution refers to the pollution phenomenon caused by the retention of trace amounts of pesticides, toxic metabolites, degradation products and impurities in biological bodies, agricultural and sideline products and the environment after the use of pesticides, which exceeds the maximum residue limit of pesticides. The toxicity of the residual pesticides to organisms is called pesticide residue, and the pesticides retained in the soil may cause pollution to the soil, atmosphere and groundwater. When pesticides are lost to the environment, the pesticides in the air are adsorbed by dust, which can cause air pollution. The pesticides in the air are also lost to the water through rainfall, causing water pollution. At the same time, the pesticides lost to the soil also cause soil compaction. Most pesticides kill all kinds of organisms without selection, including some beneficial animals such as frogs, bees and earthworms. The decrease of these beneficial animals will lead to the increase of pests, which will affect agricultural production. Wild animals and livestock will suffer acute or chronic poisoning if they eat food contaminated with pesticides, and pesticides will also affect the reproductive ability of organisms.

[0004] In view of the anthracnose of citrus, it is of great significance to develop a green, non-toxic and efficient pesticide substitute for green planting of citrus. SUMMARY

[0005] An object of the present application is to solve at least the above problems and to provide at least the advantages to be explained later.

[0006] An object of the present application is to provide a preparation method of an extract for preventing and treating citrus anthracnose, which can efficiently prevent and treat citrus anthracnose and has the characteristics of low production cost and no pollution to citrus planting environment.

[0007] In order to achieve these objects and other advantages of the present application, a preparation method of an extract for preventing and treating citrus anthracnose is provided, which comprises the following steps:

[0008] Step one, garlic, astragalus, chuanqiong, banana peel, soybean, silkworm chrysalis, and psyllium are dried and crushed, and the crushed materials are respectively added to boiling water and stirred until uniform, and then boiled for 5-10 minutes, and then frozen in an environment below -20℃, and then taken out and thawed at room temperature to obtain a freeze-thaw material;

[0009] Step two, sodium dihydrogen phosphate is added to the freeze-thaw material and mixed uniformly, and then inoculated with bacillus subtilis and bacillus mycoides for aerobic fermentation to obtain a primary fermentation material;

[0010] Step three, bacillus licheniformis, bacillus laterosporus, and bacillus thuringiensis are inoculated into the primary fermentation material for aerobic fermentation to obtain a secondary fermentation material;

[0011] Step four, the secondary fermentation material is transferred into an anaerobic tank and covered with a tarpaulin to isolate oxygen for anaerobic fermentation, and when the total acid in the fermentation material is ≥32%, the fermentation material and pure water are fully stirred and mixed at a mass ratio of 1:5, and then filtered after standing, and the filter residue and precipitate are extract A; the filtrate is adjusted to a PH value in the range of 6.5-7.5 to obtain extract B; sugarcane residue powder is added to extract A and mixed uniformly, and then compacted and packed; extract B is bottled and the bottle mouth is sealed for storage.

[0012] Preferably, the crushed materials are the following components in weight parts: garlic 35-40 parts, astragalus 30-35 parts, chuanqiong 25-35 parts, banana peel 20-30 parts, soybean 20-25 parts, silkworm chrysalis 20-25 parts, and psyllium 15-20 parts.

[0013] Preferably, the mass of sodium dihydrogen phosphate is 0.08%-0.12% of the freeze-thaw material.

[0014] Preferably, the inoculation amount of bacillus subtilis is 0.007%-0.01%, the inoculation amount of bacillus mycoides is 0.005%-0.01%, the inoculation amount of bacillus licheniformis is 0.014%-0.018%, the inoculation amount of bacillus laterosporus is 0.01%-0.02%, and the inoculation amount of bacillus thuringiensis is 0.08%-0.12%.

[0015] Preferably, in step two, after inoculation, the material is turned over at least twice to mix the Bacillus subtilis and Bacillus mycoides evenly, and aerobic fermentation is carried out for 10-20 hours, during which the material is turned over every 2.5 hours if the temperature is greater than or equal to 40 DEG C, or every 3.5 hours if the temperature is less than 40 DEG C.

[0016] Preferably, in step three, aerobic fermentation is carried out for 10-20 hours, during which the material is turned over every 2.5 hours if the temperature is greater than or equal to 40 DEG C, or every 3.5 hours if the temperature is less than 40 DEG C.

[0017] Preferably, in step one, the freezing time is at least 24 hours.

[0018] The application of the extract prepared by the preparation method in the prevention and treatment of citrus anthracnose, comprising the following steps:

[0019] When the citrus is sprouting, the extract A is applied to the root of the citrus at a dosage of 1-1.5 tons per mu, then the extract A is covered with soil, and the extract B is mixed with water at a volume ratio of 1:300 to spray the leaves;

[0020] When the citrus is not sprouting and is not diseased, the extract B is mixed with water at a volume ratio of 1:500 to spray the leaves;

[0021] When the citrus is infected with anthracnose or the tree vigor is weak, the extract B is mixed with water at a volume ratio of 1:300, then the root is irrigated, then the extract A is applied to the root at a dosage of 1-1.5 tons per mu, then the extract B is mixed with water at a volume ratio of 1:300 to spray the leaves, and the spraying is continuously carried out for 3 days.

[0022] The application at least has the following beneficial effects:

[0023] Firstly, the cellulase and proteinase produced by Bacillus subtilis and Bacillus laterosporus decompose garlic, astragalus, chuanqiong, banana peel, soybean, silkworm chrysalis, plantain seed and the like, so that the antibacterial substances and active mineral elements are released from the components, and the protein is degraded into amino acids and polypeptides; the chitin deacetylase produced by Bacillus licheniformis and Bacillus subtilis can degrade the chitin in the silkworm chrysalis into chitosan, so that the antibacterial active substances are enhanced; and the metabolic products of the above-mentioned bacteria also have the effects of antibiosis, insect killing, resistance enhancement and growth promotion.

[0024] Second, the mechanism of the extract prepared by the present application for preventing and treating citrus anthracnose is as follows: 1. The antibacterial substances produced by garlic, astragalus, etc. and bacillus subtilis, etc. inhibit the growth of anthracnose bacteria; 2. The metabolites produced by bacillus mycoides, bacillus licheniformis, etc. stimulate the immune response of crops and enhance the disease resistance of crops; 3. Banana peels, silkworm chrysalis, etc. provide rich minerals to promote the growth of crops; 4. Proteases produced by bacillus subtilis, etc. decompose the proteins contained in soybeans, silkworm chrysalis, banana peels, etc. into small molecular polypeptides and amino acids to promote the growth of citrus; 5. The insecticidal effect of bacillus thuringiensis kills insects and cuts off the transmission of anthracnose by insects. The present application enhances the resistance of citrus by enhancing the growth of citrus trees, and cuts off the transmission of anthracnose bacteria at the same time of killing and inhibiting the anthracnose bacteria, thereby greatly improving the prevention and treatment effect.

[0025] Other advantages, objects, and features of the present application will be apparent from the following specification and claims. DETAILED DESCRIPTION

[0026] The present application will be further described in detail below so that those skilled in the art can implement it according to the description.

[0027] It should be understood that the terms such as "have", "contain", and "include" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0028] It should be noted that the experimental methods in the following embodiments are conventional methods, and the reagents and materials are commercially available unless otherwise specified.

[0029] A preparation method of an extract for preventing and treating citrus anthracnose includes the following steps:

[0030] Step one: garlic, astragalus, ligusticum wallichii, banana peels, soybeans, silkworm chrysalis, and psyllium are dried and crushed, and the crushed materials are added to boiling water and stirred until uniform, and then continue to cook for 5-10 minutes, and then quickly freeze in an environment below -20℃, and then take out and thaw at room temperature to obtain freeze-thaw materials;

[0031] Step two: sodium dihydrogen phosphate is added to the freeze-thaw materials and mixed uniformly, and then inoculated with bacillus subtilis and bacillus mycoides for aerobic fermentation to obtain primary fermentation materials;

[0032] Step three: bacillus licheniformis, bacillus laterosporus, and bacillus thuringiensis are inoculated into the primary fermentation materials for aerobic fermentation to obtain secondary fermentation materials;

[0033] Step four, the secondary fermentation material is transferred into an anaerobic tank, covered with a tarp to exclude oxygen, and anaerobic fermentation is carried out; when the total acid in the fermentation material is ≥ 32%, the fermentation material and pure water are fully stirred and mixed in a mass ratio of 1:5, then filtered after standing; the filter residue and the precipitate after standing are extract A; the filtrate is adjusted to a PH value in the range of 6.5-7.5 to obtain extract B; the extract A is mixed with sugarcane residue powder, and then packed; the extract B is bottled and sealed.

[0034] Garlic contains rich natural antibacterial substances, the main component of which is allicin, which can not only prevent and control diseases and pests, but also prevent decay, and is beneficial to promoting germination and promoting the growth and development of crops.

[0035] Radix Astragali contains rich polysaccharides, which have antibacterial and bactericidal effects.

[0036] Chuanxiong has antibacterial, antioxidant, and cell protection effects, and when it is used together with garlic and Radix Astragali, it can enhance the antibacterial effect of garlic and Radix Astragali.

[0037] Banana peel is rich in protein, fat, sugar, and calcium, potassium, magnesium, iron, zinc, and more than a dozen elements. Banana peel contains a substance called peelin, which can inhibit the growth of bacteria and fungi.

[0038] Soybeans are rich in protein, unsaturated fatty acids, fiber, minerals, and vitamins.

[0039] Silkworm chrysalis contains rich protein, fat, vitamins, minerals, amino acids, ergosterols, and chitin.

[0040] Plantago contains a large amount of mucilage, succinic acid, plantaginol, adenine, choline, plantaginine, fatty oil, vitamin A, and vitamin B, and has antibacterial effects.

[0041] Bacillus subtilis can secrete protease, amylase, lipase, cellulase, pectinase, chitin deacetylase, subtilin, polymyxin, nystatin, and short bacillus peptide.

[0042] Bacillus mucilaginosus has the effects of decomposing silicate minerals, dissolving phosphorus, releasing potassium, and fixing nitrogen, and can increase the content of soluble phosphorus and potassium in the soil, improve soil fertility, improve the process of mineral elements required by crops, promote plant growth, improve disease resistance, and prevent and reduce diseases such as gray mold and anthracnose.

[0043] Bacillus licheniformis can produce bacteriolytic substances such as chitin deacetylase, cellulase, and glucanase, as well as antibacterial substances such as surfactin, iturin, and subtilin. The metabolites can also promote crop growth, induce systemic resistance in crops, and enhance the disease resistance of crops to crop pathogens.

[0044] Bacillus laterosporus can produce protease, amylase, cellulase and various vitamins, amino acids and other substances, promote crop root growth, improve root activity, and stimulate the immune system to produce various immune responses, enhance the disease resistance of crops; Bacillus laterosporus can also secrete insecticidal and antibacterial active substances.

[0045] Bacillus thuringiensis can produce spore-associated crystal toxins and cause insect toxicemia and death.

[0046] The present application utilizes the cellulase, protease and other substances produced by Bacillus subtilis, Bacillus laterosporus and the like to decompose garlic, astragalus, chuanqiong, banana peel, soybean, silkworm chrysalis, plantain seed and other substances, so that the above components release antibacterial substances and active mineral elements, and the proteins are degraded into amino acids and polypeptides; the chitin deacetylase produced by Bacillus licheniformis and Bacillus subtilis can degrade the chitin in silkworm chrysalis into chitosan, enhancing the antibacterial active substances. And the metabolic products of the above-mentioned bacteria also have the effects of antibiosis, insecticidal, resistance enhancement and growth promotion. The mechanism of the extract prepared by the present application for preventing and treating citrus anthracnose is as follows: 1. Utilizing the antibacterial substances produced by garlic, astragalus and the like and Bacillus subtilis and the like to inhibit the growth of anthracnose bacteria; 2. Utilizing the metabolic products produced by Bacillus mycoides, Bacillus licheniformis and the like to stimulate crops to produce immune responses and enhance the disease resistance of crops; 3. Utilizing banana peel, silkworm chrysalis and the like to provide rich minerals and promote the growth of crops; 4. Utilizing the protease produced by Bacillus subtilis and the like to decompose the proteins contained in soybean, silkworm chrysalis, banana peel and the like into small molecular polypeptides and amino acids, promoting the growth of citrus; 5. Utilizing the insecticidal effect of Bacillus thuringiensis to kill insects and cut off the transmission of anthracnose disease by insects.

[0047] In another technical solution, the pulverized material is composed of the following components in weight parts: garlic 35-40 parts, astragalus 30-35 parts, chuanqiong 25-35 parts, banana peel 20-30 parts, soybean 20-25 parts, silkworm chrysalis 20-25 parts, and plantain seed 15-20 parts.

[0048] In another technical solution, the mass of sodium dihydrogen phosphate is 0.08%-0.12% of the freeze-thaw material.

[0049] In another technical solution, the inoculation amount of Bacillus subtilis is 0.007%-0.01%, the inoculation amount of Bacillus mycoides is 0.005-0.01%, the inoculation amount of Bacillus licheniformis is 0.014%-0.018%, the inoculation amount of Bacillus laterosporus is 0.01-0.02%, and the inoculation amount of Bacillus thuringiensis is 0.08%-0.12%.

[0050] In another technical solution, in step two, after inoculation, the material is mixed evenly with Bacillus subtilis and Bacillus mycoides by turning over at least twice, aerobic fermentation is carried out for 10-20 hours, and the material is turned over every 2.5 hours when the temperature is greater than or equal to 40℃, or every 3.5 hours when the temperature is less than 40℃.

[0051] In another technical solution, in step three, aerobic fermentation is carried out for 10-20 hours, and the material is turned over every 2.5 hours when the temperature is greater than or equal to 40℃, or every 3.5 hours when the temperature is less than 40℃.

[0052] In another technical solution, in step one, the freezing time is at least 24 hours.

[0053] In another technical solution, the extract A is further processed by the following steps:

[0054] 1) 5-10% bagasse powder is added to the extract A, and the mixture is evenly mixed to obtain material A;

[0055] 2) Potassium feldspar, phosphate powder, dopamine hydrochloride, and genipin are added to the material A, and the mass ratio of the material A to the potassium feldspar, phosphate powder, 10% (wt) dopamine hydrochloride, and 0.1% (wt) genipin is 100:1-5:1-5:5-10:1-5:0.1-1, and the mixture is evenly mixed to obtain material B;

[0056] 2) Bagasse powder is added to the material B, and the mass ratio of the material B to the bagasse powder is 100:1-10, and the mixture is evenly mixed and granulated in a granulator, and the granular material is dried at 40℃ to obtain granular material A.

[0057] The cellulose in the bagasse powder is rich in hydroxyl groups and has good adsorption performance. In this embodiment, the bagasse powder is mixed with the extract A to utilize the adsorption of the bagasse powder on the nutrient components and bacterial bodies such as Bacillus subtilis in the extract A, and then the bagasse powder is mixed with potassium feldspar, phosphate powder, dopamine hydrochloride, and genipin, and the bagasse powder is used to adjust the moisture content for granulation in the granulator. During the subsequent drying process of the granules, the cross-linking of chitosan, dopamine hydrochloride, and genipin forms a cross-linked structure on the surface or inside of the granular material A to bind the bacterial bodies, potassium feldspar, phosphate powder, and various nutrient components in the granular material A, so that the granular material A has a slow-release effect during use, avoiding the loss of nutrients, and creating a suitable microenvironment for the growth of bacterial bodies such as Bacillus subtilis, so that the bacteria become dominant in the microenvironment, and the bacterial bodies maintain a strong growth ability for a long time, thereby improving the control effect of the bacterial bodies. The created microenvironment can increase the concentration of local microbial metabolites, enhance the effects of dissolving phosphorus and potassium, and continuously provide sufficient mineral elements for the growth of citrus, avoiding the occurrence of tree weakness.

[0058] In another technical solution, the extract B is further treated before use by adding the following raw material components into 100 parts by volume of the extract B: 0.5-1 parts by weight of dopamine hydrochloride, 3-5 parts by weight of montmorillonite powder and 0.01-0.1 parts by weight of genipin, and then mixing the extract B with the dopamine hydrochloride to obtain an application liquid B.

[0059] The extract B contains rich polypeptides, amino acids, secondary metabolites of bacillus subtilis, chitosan formed by deacetylation of chitin, and rich mineral elements such as potassium, phosphorus, calcium, etc. The cross-linking of dopamine hydrochloride, chitosan and genipin in the present embodiment enables the extract B to be cross-linked on the surface of crops after spraying, to form an antibacterial layer and a nutrient slow-release layer on the surface of crops, thereby enhancing the disease resistance of crops.

[0060] An application of the extract prepared by the preparation method in the prevention and treatment of citrus anthracnose, which comprises the following steps:

[0061] When the citrus is sprouting, the granular material A is applied to the roots of the citrus at a dosage of 1-1.5 tons per mu, then the granular material A is covered with soil, and the application liquid B is mixed with water at a volume ratio of 1:300 to spray the leaves;

[0062] When the citrus is not sprouting and is not diseased, the application liquid B is mixed with water at a volume ratio of 1:500 to spray the leaves;

[0063] When the citrus is infected with anthracnose or the tree vigor is weakened, the application liquid B is mixed with water at a volume ratio of 1:300, then the roots are irrigated, the leaves are sprayed, the granular material A is applied to the roots at a dosage of 1-1.5 tons per mu, the application liquid B is mixed with water at a volume ratio of 1:300 to spray the leaves, and the spraying is continuously performed for 3 days.

[0064] An application of the extract prepared by the preparation method in the prevention and treatment of citrus anthracnose, which comprises the following steps:

[0065] When the citrus is sprouting, the granular material A is applied to the roots of the citrus at a dosage of 1-1.5 tons per mu, then the granular material A is covered with soil, and the application liquid B is mixed with water at a volume ratio of 1:300 to spray the leaves;

[0066] When the citrus is not sprouting and is not diseased, the application liquid B is mixed with water at a volume ratio of 1:500 to spray the leaves;

[0067] When the citrus is infected with anthracnose or the tree vigor is weakened, the application liquid B is mixed with water at a volume ratio of 1:300, then the roots are irrigated, the leaves are sprayed, the granular material A is applied to the roots at a dosage of 1-1.5 tons per mu, the application liquid B is mixed with water at a volume ratio of 1:300 to spray the leaves, and the spraying is continuously performed for 3 days.

[0068] <Example 1>

[0069] A preparation method and application of an extractive for preventing and treating citrus anthracnose, comprising the following steps:

[0070] Step one, after drying garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and psyllium, they are crushed, and the following components are taken respectively: garlic 35 parts, astragalus 30 parts, ligusticum wallichii 25 parts, banana peel 20 parts, soybean 20 parts, silkworm chrysalis 20 parts, and psyllium 15 parts, which are added into 150 parts of boiling water and stirred evenly, then continue to boil for 5 minutes, and then quickly freeze in an environment below -20℃, after freezing, take out and thaw in room temperature environment, the freezing time is 24 hours, and the freeze-thaw material is obtained;

[0071] Step two, sodium dihydrogen phosphate is added into the freeze-thaw material, and the mass of sodium dihydrogen phosphate is 0.08% of the freeze-thaw material, and they are mixed evenly, then inoculated with bacillus subtilis and bacillus mycoides for aerobic fermentation, the inoculation amount of bacillus subtilis is 0.007%, and the inoculation amount of bacillus mycoides is 0.005%, and the first fermentation material is obtained; wherein, after inoculation, the material is turned over twice to mix the material with bacillus subtilis and bacillus mycoides evenly, and aerobic fermentation is carried out for 20 hours, if the material temperature is greater than or equal to 40℃, the material is turned over every 2.5 hours, and if the material temperature is less than 40℃, the material is turned over every 3.5 hours;

[0072] Step three, bacillus licheniformis, bacillus laterosporus and bacillus thuringiensis are inoculated into the first fermentation material for aerobic fermentation, the inoculation amount of bacillus licheniformis is 0.014%, the inoculation amount of bacillus laterosporus is 0.01%, and the inoculation amount of bacillus thuringiensis is 0.08%, aerobic fermentation is carried out for 20 hours, if the material temperature is greater than or equal to 40℃, the material is turned over every 2.5 hours, and if the material temperature is less than 40℃, the material is turned over every 3.5 hours, and the second fermentation material is obtained;

[0073] Step four, the second fermentation material is transferred into an anaerobic tank, and the tank is covered with a tarpaulin to isolate oxygen, and anaerobic fermentation is carried out, when the total acid in the fermentation material is greater than or equal to 32%, the fermentation material and pure water are fully stirred according to the mass ratio of 1:5, and after standing, filtration is carried out, the filter residue and the precipitate after standing are the extractive A; the filtrate is adjusted to a PH value in the range of 6.5 to obtain the extractive B; the extractive A is mixed with sugarcane residue powder, and then is compacted and packed in a bag; the extractive B is bottled and the bottle opening is sealed for storage;

[0074] Step five, when citrus shoots, the extractive A is applied at the root of the citrus, and the amount is 1 ton per mu, then the extractive A is covered with soil, and the extractive B is mixed with water according to the volume ratio of 1:300, and then the mixture is sprayed on the leaves;

[0075] When there is no citrus shoot and no disease, the extractive B is mixed with water according to the volume ratio of 1:500, and then the mixture is sprayed on the leaves;

[0076] When citrus is infected with anthracnose or the tree vigor is weak, extract B and water are mixed uniformly at a volume ratio of 1:300, then the roots are irrigated, then extract A is applied to the roots at a dosage of 1 ton / acre, then extract B and water are mixed uniformly at a volume ratio of 1:300, and the leaves are sprayed, and the spraying is continuously performed for 3 days.

[0077] <Embodiment 2>

[0078] A preparation method and application of an extract for preventing and treating citrus anthracnose, comprising the following steps:

[0079] Step one, garlic, astragalus, chuanxiong, banana peel, soybeans, silkworm chrysalis, and psyllium are dried and crushed, and the following components are taken in the following weight portions: garlic 40 portions, astragalus 35 portions, chuanxiong 35 portions, banana peel 30 portions, soybeans 25 portions, silkworm chrysalis 25 portions, and psyllium 20 portions, which are added to 200 portions of boiling water and stirred uniformly, and then boiled for 10 minutes, and then quickly frozen in an environment of minus 25℃, and then taken out and thawed at room temperature, and the freezing time is 24 hours, and a freeze-thaw material is obtained;

[0080] Step two, sodium dihydrogen phosphate is added to the freeze-thaw material, and the mass of the sodium dihydrogen phosphate is 0.12% of the mass of the freeze-thaw material, and the mixture is placed in a fermentation tank and inoculated with bacillus subtilis and bacillus mycoides for aerobic fermentation, and the inoculation amount of the bacillus subtilis is 0.01%, and the inoculation amount of the bacillus mycoides is 0.01%, and a first fermentation material is obtained; wherein, after inoculation, the material is turned over twice to mix the material with the bacillus subtilis and the bacillus mycoides uniformly, and aerobic fermentation is performed for 10 hours, and if the material temperature is greater than or equal to 40℃, the material is turned over every 2.5 hours, and if the material temperature is less than 40℃, the material is turned over every 3.5 hours;

[0081] Step three, bacillus licheniformis, bacillus laterosporus, and bacillus thuringiensis are inoculated into the first fermentation material for aerobic fermentation, and the inoculation amount of the bacillus licheniformis is 0.018%, the inoculation amount of the bacillus laterosporus is 0.02%, and the inoculation amount of the bacillus thuringiensis is 0.12%, and aerobic fermentation is performed for 10 hours, and if the material temperature is greater than or equal to 40℃, the material is turned over every 2.5 hours, and if the material temperature is less than 40℃, the material is turned over every 3.5 hours, and a second fermentation material is obtained;

[0082] Step four, the second fermentation material is transferred to an anaerobic tank, and a tarpaulin is covered to isolate oxygen, and anaerobic fermentation is performed, and when the total acid in the fermentation material is greater than or equal to 32%, the fermentation material and pure water are stirred uniformly at a mass ratio of 1:5, and then filtered after standing, and the filter residue and the precipitate after standing are extract A; the filtrate is adjusted to a PH value in the range of 7.5 to obtain extract B; and the extract A is mixed with sugarcane residue powder to obtain a mixture, and then the mixture is compacted and packed in a bag; and the extract B is bottled and sealed at the bottle opening for storage;

[0083] Step five, when the citrus shoots, citrus root application of extract A, the amount of 1.5 tons per mu, then add soil cover extract A, and extract B and water are mixed uniformly according to the volume ratio of 1:300, spraying on the leaves; when the citrus shoots and no disease, extract B and water are mixed uniformly according to the volume ratio of 1:500, spraying on the leaves; when the citrus infected with anthracnose or tree vigor, extract B and water are mixed uniformly according to the volume ratio of 1:300, first irrigation, then the root application of extract A, the amount of 1.5 tons per mu, then extract B and water are mixed uniformly according to the volume ratio of 1:300, spraying on the leaves, continuous spraying for 3 days.

[0084] <Example 3>

[0085] A preparation method and application of an extract for preventing and treating citrus anthracnose, comprising the following steps:

[0086] Step one, garlic, astragalus, chuanxiong, banana peel, soybean, silkworm chrysalis, psyllium are dried and crushed, and the following components are taken respectively: 35 parts of garlic, 30 parts of astragalus, 25 parts of chuanxiong, 20 parts of banana peel, 20 parts of soybean, 20 parts of silkworm chrysalis, and 15 parts of psyllium, which are added to 150 parts of boiling water and stirred uniformly, then continue to cook for 5 minutes, and then quickly freeze in an environment of minus 20℃, after freezing, take out and thaw at room temperature, the freezing time is 24 hours, and the freeze-thaw material is obtained;

[0087] Step two, sodium dihydrogen phosphate is added to the freeze-thaw material, the mass of sodium dihydrogen phosphate is 0.08% of the freeze-thaw material, and the mixture is placed in a fermentation tank and inoculated with bacillus subtilis and bacillus mycoides for aerobic fermentation, the inoculation amount of bacillus subtilis is 0.007%, and the inoculation amount of bacillus mycoides is 0.005%, and the primary fermentation material is obtained; wherein, after inoculation, the material is stirred for 2 times to mix the material with bacillus subtilis and bacillus mycoides uniformly, aerobic fermentation is carried out for 20 hours, if the material temperature is greater than or equal to 40℃, the material is stirred every 2.5 hours, and if the material temperature is less than 40℃, the material is stirred every 3.5 hours;

[0088] Step three, bacillus licheniformis, bacillus laterosporus and bacillus thuringiensis are inoculated into the primary fermentation material for aerobic fermentation, the inoculation amount of bacillus licheniformis is 0.014%, the inoculation amount of bacillus laterosporus is 0.01%, and the inoculation amount of bacillus thuringiensis is 0.08%, aerobic fermentation is carried out for 20 hours, if the material temperature is greater than or equal to 40℃, the material is stirred every 2.5 hours, and if the material temperature is less than 40℃, the material is stirred every 3.5 hours, and the secondary fermentation material is obtained;

[0089] Step four, the secondary fermentation material is transferred into an anaerobic tank, a tarpaulin is covered to isolate oxygen, anaerobic fermentation is carried out, when the total acid in the fermentation material is greater than or equal to 32%, the fermentation material and pure water are fully stirred and mixed uniformly according to a mass ratio of 1:5, after standing, filtration is carried out, the filtered residue and the standing precipitate are extract A; the filtrate is adjusted to a PH value in a range of 6.5 to obtain extract B;

[0090] Step five, 5% of sugarcane residue powder by mass fraction is added into the extract A and mixed uniformly to obtain material A; potash feldspar, phosphate powder, dopamine hydrochloride and genipin are added into the material A, the mass ratio of the material A to the potash feldspar, the phosphate powder, the dopamine hydrochloride and the genipin is 100:1:1:5:1:0.1, and the mixture is mixed uniformly to obtain material B; the material B is mixed with sugarcane residue powder, the mass ratio of the material B to the sugarcane residue powder is 100:1, and the mixture is granulated in a granulator, and the granular material is dried at 45℃ to obtain granular material A;

[0091] Step six, 0.5 parts of dopamine hydrochloride, 3 parts of montmorillonite powder and 0.01 parts of genipin are added into 100 parts of extract B by volume to obtain application liquid B.

[0092] Step seven, when the citrus is sprouting, the granular material A is applied to the root of the citrus at a dosage of 1 ton per mu, then the granular material A is covered with soil, the application liquid B is mixed with water uniformly at a volume ratio of 1:300, and the leaves are sprayed; when the citrus is not sprouting and is not infected, the application liquid B is mixed with water uniformly at a volume ratio of 1:500, and the leaves are sprayed; when the citrus is infected with anthracnose or the tree vigor is weakened, the application liquid B is mixed with water uniformly at a volume ratio of 1:300, the root is irrigated first, then the granular material A is applied to the root at a dosage of 1 ton per mu, the application liquid B is mixed with water uniformly at a volume ratio of 1:300, and the leaves are sprayed, and the spraying is continuously carried out for 3 days.

[0093] <Example 4>

[0094] A preparation method and application of an extract for preventing and treating citrus anthracnose include the following steps:

[0095] Step one, garlic, astragalus, chuanxiong, banana peel, soybeans, silkworm chrysalis and plantain seeds are dried and crushed, the following components are taken respectively by weight: 40 parts of garlic, 35 parts of astragalus, 35 parts of chuanxiong, 30 parts of banana peel, 25 parts of soybeans, 25 parts of silkworm chrysalis and 20 parts of plantain seeds, and the components are added into 200 parts of boiling water and stirred uniformly, then the mixture is continuously boiled for 10 minutes, and then quickly frozen in an environment at minus 25℃, after the freezing is completed, the mixture is taken out and thawed in a room temperature environment, the freezing time is 24 hours, and a freeze-thaw material is obtained.

[0096] Step two, the frozen and thawed material is mixed with sodium dihydrogen phosphate, the mass of sodium dihydrogen phosphate is 0.12% of the frozen and thawed material, and then the mixture is placed in a fermentation tank, inoculated with Bacillus subtilis and Bacillus mycoides for aerobic fermentation, the inoculation amount of Bacillus subtilis is 0.01%, and the inoculation amount of Bacillus mycoides is 0.01%, to obtain a first fermentation material; wherein, after inoculation, the material is turned over 2 times to mix the material with Bacillus subtilis and Bacillus mycoides uniformly, and aerobic fermentation is carried out for 10 hours, during which the material is turned over every 2.5 hours if the temperature is greater than or equal to 40℃, or every 3.5 hours if the temperature is less than 40℃;

[0097] Step three, Bacillus licheniformis, Bacillus laterosporus and Bacillus thuringiensis are inoculated into the first fermentation material for aerobic fermentation, the inoculation amount of Bacillus licheniformis is 0.018%, the inoculation amount of Bacillus laterosporus is 0.02%, and the inoculation amount of Bacillus thuringiensis is 0.12%, aerobic fermentation is carried out for 10 hours, during which the material is turned over every 2.5 hours if the temperature is greater than or equal to 40℃, or every 3.5 hours if the temperature is less than 40℃, to obtain a second fermentation material;

[0098] Step four, the second fermentation material is transferred to an anaerobic tank, covered with a tarpaulin to isolate oxygen, and anaerobic fermentation is carried out, when the total acid in the fermentation material is greater than or equal to 32%, the fermentation material and pure water are mixed in a mass ratio of 1:5, stirred uniformly, and then filtered after standing, the filter residue and the precipitate after standing are extract A; the filtrate is adjusted to a PH value in the range of 7.5 to obtain extract B;

[0099] Step five, 10% of sugarcane bagasse powder by mass fraction is added to extract A to obtain material A; potassium feldspar, phosphate rock powder, dopamine hydrochloride and genipin are added to material A, the mass ratio of material A to potassium feldspar, phosphate rock powder, dopamine hydrochloride and genipin is 100:5:5:10:5:1, and then the mixture is mixed uniformly to obtain material B; sugarcane bagasse powder is added to material B, the mass ratio of material B to sugarcane bagasse powder is 100:10, and then the mixture is mixed uniformly, placed in a granulator, and dried at 45℃ to obtain granular material A;

[0100] Step six, 1 part of dopamine hydrochloride, 5 parts of montmorillonite powder and 0.1 part of genipin are added to 100 parts by volume of extract B to obtain application liquid B.

[0101] Step seven, when the citrus shoots, at the root of the citrus granules A, the amount of 1.5 tons per mu, then add soil covering granules A, and the application of liquid B mixed with water in a volume ratio of 1:300 uniform, spraying on the leaves; non-citrus shoots and not when the disease, the application of liquid B mixed with water in a volume ratio of 1:500 uniform, spraying on the leaves; citrus infected anthracnose or tree weakened, the application of liquid B and water in a volume ratio of 1:300 mixed uniform, first irrigation, then at the root of the granules A, the amount of 1.5 tons per mu, then use the application of liquid B and water in a volume ratio of 1:300 mixed uniform, spraying on the leaves, continuous spraying 3 days.

[0102] <Comparative test>

[0103] Before the prevention of the disease base investigation, and 14 days after the last application of investigation; leaf anthracnose control effect investigation: each mu randomly investigate 2 trees, each according to the east, west, south, north five directions fixed 2 new shoots, investigation of all leaves, record the total leaf number, the number of each level of disease leaves, statistics disease index and control effect.

[0104] Leaf classification standards:

[0105] 0 level: no disease spot;

[0106] 1 level: disease spot area accounts for 5% or less of the entire leaf area;

[0107] 3 level: disease spot area accounts for 6%-10% or less of the entire leaf area;

[0108] 5 level: disease spot area accounts for 11%-25% or less of the entire leaf area;

[0109] 7 level: disease spot area accounts for 26%-50% or less of the entire leaf area;

[0110] 9 level: disease spot area accounts for 51% or more of the entire leaf area.

[0111] Disease index and control effect calculation:

[0112] Disease index = ∑ (each level of disease leaf number x relative level value) / (survey total leaf number x 9) x 100;

[0113] Control effect (%) = (control area disease index - treatment area disease index) / control area disease index x 100.

[0114] Comparative example 1: the method of example 1 is used to prepare extract A and extract B, the difference is that the component does not contain silkworm chrysalis.

[0115] Comparative example 2: the method of example 1 is used to prepare extract A and extract B, the difference is that the component does not contain chuanxiong, psyllium.

[0116] Comparative Example 3: Extract A and extract B were prepared by the method of Example 1, except that Bacillus subtilis and Bacillus licheniformis were not contained in the fermentation broth.

[0117] Comparative Example 4: Extract A and extract B were prepared by the method of Example 1, except that Bacillus thuringiensis was not contained in the fermentation broth.

[0118] Comparative Example 5: Granular A and application liquid B were prepared by the method of Example 1, except that Bacillus mycoides was not contained in the components.

[0119] Comparative Example 6: Granular A and application liquid B were prepared by the method of Example 3, except that montmorillonite powder was not contained in the components.

[0120] <Effect of disease prevention and control>

[0121] In the Valencia orange orchard where anthracnose disease occurred, 7 mu were randomly selected and marked as A, B, C, D, E, F, G, H, and I;

[0122] For A, extract A and application liquid B prepared by Comparative Example 1 were used, extract B and water were mixed at a volume ratio of 1:300 to prepare drip irrigation liquid, the drip irrigation liquid was used to irrigate the roots, then extract A was applied to the roots at a dosage of 1.5 tons per mu, and then extract B and water were mixed at a volume ratio of 1:300 to evenly spray the leaves, and the spraying was continuously performed for 3 days;

[0123] For B, extract A and application liquid B prepared by Comparative Example 2 were used, extract B and water were mixed at a volume ratio of 1:300 to prepare drip irrigation liquid, the drip irrigation liquid was used to irrigate the roots, then extract A was applied to the roots at a dosage of 1.5 tons per mu, and then extract B and water were mixed at a volume ratio of 1:300 to evenly spray the leaves, and the spraying was continuously performed for 3 days;

[0124] For C, extract A and application liquid B prepared by Comparative Example 3 were used, extract B and water were mixed at a volume ratio of 1:300 to prepare drip irrigation liquid, the drip irrigation liquid was used to irrigate the roots, then extract A was applied to the roots at a dosage of 1.5 tons per mu, and then extract B and water were mixed at a volume ratio of 1:300 to evenly spray the leaves, and the spraying was continuously performed for 3 days;

[0125] For D, extract A and application liquid B prepared by Comparative Example 4 were used, extract B and water were mixed at a volume ratio of 1:300 to prepare drip irrigation liquid, the drip irrigation liquid was used to irrigate the roots, then extract A was applied to the roots at a dosage of 1.5 tons per mu, and then extract B and water were mixed at a volume ratio of 1:300 to evenly spray the leaves, and the spraying was continuously performed for 3 days;

[0126] For E area, the extract A prepared by comparative example 5 and the application liquid B, the extract B and water were mixed in a volume ratio of 1:300 to prepare the drip irrigation liquid, the drip irrigation liquid was used to irrigate the roots, then the extract A was applied to the roots with an amount of 1.5 tons per mu, and the extract B and water were mixed in a volume ratio of 1:300 to spray the leaves uniformly, and the spraying was continuously performed for 3 days;

[0127] For F area, the application liquid B and water were mixed in a volume ratio of 1:300 to prepare the drip irrigation liquid, the drip irrigation liquid was used to irrigate the roots, then the granular material A was applied to the roots with an amount of 1.5 tons per mu, and the application liquid B and water were mixed in a volume ratio of 1:300 to spray the leaves uniformly, and the spraying was continuously performed for 3 days;

[0128] For G area, the granular material A prepared by comparative example 6 and the application liquid B, the application liquid B and water were mixed in a volume ratio of 1:300 to prepare the drip irrigation liquid, the drip irrigation liquid was used to irrigate the roots, then the granular material A was applied to the roots with an amount of 1.5 tons per mu, and the application liquid B and water were mixed in a volume ratio of 1:300 to spray the leaves uniformly, and the spraying was continuously performed for 3 days;

[0129] For H area, the granular material A prepared by example 3 and the application liquid B, the application liquid B and water were mixed in a volume ratio of 1:300 to prepare the drip irrigation liquid, the drip irrigation liquid was used to irrigate the roots, then the granular material A was applied to the roots with an amount of 1.5 tons per mu, and the application liquid B and water were mixed in a volume ratio of 1:300 to spray the leaves uniformly, and the spraying was continuously performed for 3 days;

[0130] The roots of the citrus trees in G area were irrigated with water, and the fermented material prepared by fermenting cow dung was applied to the roots with an amount of 1.5 tons per mu, and then the leaves were sprayed continuously for 3 days. The leaf control effect after 14 days is shown in Table 1.

[0131] Table 1 Field anthracnose control effect

[0132]

[0133]

[0134] From the results in Table 1, it can be seen that the control effect of A area is lower than that of F area, indicating that increasing silkworm chrysalis can improve the control effect of anthracnose; the control effect of B area is lower than that of F area, indicating that increasing chuanqiong and plantain seed can improve the control effect of anthracnose; the control effect of C area is lower than that of F area, indicating that increasing bacillus subtilis and bacillus licheniformis can improve the control effect of anthracnose; the control effect of D area is lower than that of F area, indicating that increasing bacillus thuringiensis can improve the control effect of anthracnose; the control effect of E area is lower than that of F area, indicating that increasing bacillus mycoides can improve the control effect of anthracnose; the control effect of H area is better than that of G area, indicating that increasing montmorillonite powder can improve the control effect of anthracnose.

[0135] <Effect test>

[0136] Test one: from February 27, 2022, in the Lingqi Wohuan base in Luoxu Town, Wuming District, Nanning City, 4 mu of Wohuan planted in the base were randomly selected and divided into 4 parts, and the methods provided in Examples 1, 2, 3 and 4 were used to prevent and treat anthracnose, respectively. By June 30, 2022, the 4 mu of Wohuan trees in the test did not develop anthracnose, while 17 mu of Wohuan trees in the base developed anthracnose. By October 30, 2022, the Wohuan trees treated with the methods provided in Examples 1 and 2 developed anthracnose, while the Wohuan trees treated with the methods provided in Examples 3 and 4 did not develop anthracnose. Four mu of the 17 mu of Wohuan trees that developed anthracnose were randomly selected and divided into 4 parts, each part being 1 mu, and the methods provided in Examples 1, 2, 3 and 4 were used to treat anthracnose, respectively. The tree vigor of all parts recovered in 3 days, while the other 13 mu of Wohuan trees infected with anthracnose treated with prochloraz spray recovered in 5-15 days. Although prochloraz can prevent and treat anthracnose, the prevention and treatment effect is not ideal, and anthracnose occurs repeatedly within 10 months. However, the methods provided in Examples 1, 2, 3 and 4 do not cause anthracnose to occur repeatedly.

[0137] Test two: from February 28, 2022, in Guangxi Yalin Fruit Industry, 4 mu of sugar orange trees planted in the base were randomly selected and divided into 4 parts, and the methods provided in Examples 1, 2, 3 and 4 were used to prevent and treat anthracnose, respectively. By June 30, 2022, the 4 mu of sugar orange trees in the test did not develop anthracnose, while 22 mu of sugar orange trees in the base developed anthracnose. By October 30, 2022, the sugar orange trees treated with the methods provided in Examples 1 and 2 developed anthracnose, while the sugar orange trees treated with the methods provided in Examples 3 and 4 did not develop anthracnose. Four mu of the 22 mu of sugar orange trees that developed anthracnose were randomly selected and divided into 4 parts, each part being 1 mu, and the methods provided in Examples 1, 2, 3 and 4 were used to treat anthracnose, respectively. The tree vigor of all parts recovered in 3 days, while the other 18 mu of sugar orange trees treated with traditional prochloraz recovered in 5-12 days. Although prochloraz can prevent and treat anthracnose, the prevention and treatment effect is not ideal, and anthracnose occurs repeatedly within 10 months, seriously reducing the appearance of the fruit. When the methods provided in Examples 1, 2, 3 and 4 are used to prevent and treat citrus anthracnose throughout the base, the sugar orange trees grow well, and the sugar orange trees can be put on the market in mid-October, while the sugar orange trees treated with traditional methods cannot be put on the market until 2 months later.

[0138] Test three, from February 8, 2022, in Guangxi Hongjiang Orange Orchard located in Chongzuo Tendeng County, Guangxi, 4 mu of Hongjiang orange planted in the orchard were selected and divided into 4 parts, and the methods provided in example 1, example 2, example 3, and example 4 were used to prevent and control anthracnose, respectively. Until June 30, 2022, the 4 mu of Hongjiang orange in the test did not have anthracnose, while 21 mu of Hongjiang orange trees in the base had anthracnose. Until October 30, 2022, the Hongjiang orange trees treated with the methods provided in example 1 and example 2 had anthracnose, and the Hongjiang orange trees treated with the methods provided in example 3 and example 4 did not have anthracnose. Among the 21 mu of Hongjiang orange trees with anthracnose, 4 mu were selected and divided into 4 parts, and the methods of example 1, example 2, example 3, and example 4 were used to treat anthracnose, respectively. After 5 days, the tree vigor of all the trees was restored. However, the other 17 mu of Hongjiang orange trees were treated with the traditional method of prochloraz, and it took 7-20 days for the tree vigor to recover.

[0139] Although the embodiments of the present application have been disclosed as above, they are not limited to the use listed in the specification and embodiments, and can be fully applied to various fields suitable for the present application, and additional modifications can be easily made by those skilled in the art, and therefore the present application is not limited to specific details, but is within the general concept defined by the claims and equivalent scope.

Claims

1. A method for the preparation of an extract for the control of anthracnose of citrus, characterized in that, It comprises the following steps: Step one, garlic, astragalus, chuanxiong, banana peel, soybean, silkworm chrysalis, psyllium after drying and crushing, respectively taking the above crushed material, adding to boiling water and stirring evenly, continuing to cook for 5-10 min, then freezing in an environment below-20℃, after freezing, taking out and thawing at room temperature, obtaining freeze-thaw material; The crushed material is the following components by weight: garlic 35-40 parts, astragalus 30-35 parts, chuanxiong 25-35 parts, banana peel 20-30 parts, soybean 20-25 parts, silkworm chrysalis 20-25 parts, and psyllium 15-20 parts; Step two, adding sodium dihydrogen phosphate to the freeze-thaw material and mixing evenly, inoculating bacillus subtilis and bacillus mycoides in a fermentation tank for aerobic fermentation to obtain primary fermented material; After inoculation, the material is mixed evenly with bacillus subtilis and bacillus mycoides by at least 2 times of turning and throwing, aerobic fermentation for 10-20 h, and turning every 2.5 h when the material temperature is greater than or equal to 40℃, or every 3.5 h when the material temperature is less than 40℃ Step three, inoculating bacillus licheniformis, bacillus laterosporus and bacillus thuringiensis in the primary fermented material for aerobic fermentation to obtain secondary fermented material; After inoculation, the material is mixed evenly with bacillus subtilis and bacillus mycoides by at least 2 times of turning and throwing, aerobic fermentation for 10-20 h, and turning every 2.5 h when the material temperature is greater than or equal to 40℃, or every 3.5 h when the material temperature is less than 40℃ Step four, transferring the secondary fermented material into an anaerobic tank, covering the cloth to isolate oxygen, and carrying out anaerobic fermentation, when the total acid in the fermented material is greater than or equal to 32%, taking the fermented material and pure water according to the mass ratio of 1:5, stirring evenly, standing, and filtering, the filter residue and precipitate being extract A, the filtrate being adjusted to pH 6.5-7.5 to obtain extract B, adding bagasse powder to extract A and mixing evenly, then compacting and packaging, and storing the bottled extract B after sealing the bottle mouth; 2. The method of preparing the extract for controlling the anthracnose of citrus according to claim 1, characterized in that, The inoculation amount of bacillus subtilis is 0.007%-0.01%, the inoculation amount of bacillus mycoides is 0.005%-0.01%, the inoculation amount of bacillus licheniformis is 0.014%-0.018%, the inoculation amount of bacillus laterosporus is 0.01%-0.02%, and the inoculation amount of bacillus thuringiensis is 0.08%-0.12%.

3. The method of claim 1, wherein the extract for controlling anthracnose of citrus is prepared by the steps of: The added amount of sodium dihydrogen phosphate is 0.08%-0.12% of the freeze-thaw material. ​ 4. Use of the extract prepared by the process according to any one of claims 1-3 for the control of anthracnose in citrus, characterized in that, In step one, the freezing time is at least 24 h. It comprises the following steps: When the citrus is sprouting, applying extract A at the root of the citrus at a dosage of 1-1.5 tons per mu, then covering the extract A with soil, mixing extract B with water according to the volume ratio of 1:300, and spraying the mixture on the leaves; When the citrus is not sprouting and is not diseased, mixing extract B with water according to the volume ratio of 1:500, and spraying the mixture on the leaves; When the citrus is infected with anthracnose or the tree vigor is weak, mixing extract B with water according to the volume ratio of 1:300, first irrigating the root, then applying extract A at the root at a dosage of 1-1.5 tons per mu, mixing extract B with water according to the volume ratio of 1:300, and spraying the mixture on the leaves, and continuously spraying for 3 days.

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