37 results about "Purpureocillium lilacinum" patented technology

The invention discloses a bio-control composite bacterial agent which comprises, by weight, bacterial strains including 8-12 parts of purpureocillium lilacinum, 18-22 parts of pseudomonas fluorescens, 18-22 parts of bacillus cereus, 8-12 parts of bacillus mucilaginosus, 18-22 parts of bacillus subtilis, 8-12 parts of bacillus megaterium, 4-6 parts of bacillus thuringiensis and 4-6 parts of trichoderma viride and a culture medium comprising 0.8-1.2 parts of beef extract, 2.5-4 parts of potassium dihydrogen phosphate, 1.3-1.8 parts of urea, 10-12 parts of molasses, 1.5-2.5 parts of amino acid, 2.5-3.5 parts of glucose, 0.3-0.6 part of peptone, 0.25-0.4 part of sodium chloride and 0.35-0.6 part of potassium nitrate. The invention further comprises a preparation method of the bacterial agent. The bio-control composite bacterial agent has good effect on solving current problems of soil improvement, environment protection of agriculture and waste straw rubbish and can improve soil and accelerate crop growth and organic matter decomposition.

The present invention relates to a fungi mixing bacterial agent for preventing and controlling plant meloidogyne diseases, and applications thereof, wherein two fungi cultures such as Purpureocillium lilacinum CGMCC No.9344 and Clonostachys rosea CGMCC No.1037 are cultured to prepare the fungi mixing bacterial agent. According to the present invention, the fungi mixing bacterial agent can be mixed with seedling breeding mediums, organic fertilizers and organic-inorganic compound fertilizers or be used separately so as to perform soil treatment or hole application before or after transplantation, or root-irrigation, and is used for preventing and controlling plant meloidogyne diseases.

The invention relates to purpureocillium lilacinum with strong virulence on cucumber root-knot nematodes and an application thereof. The strain is Pl36-1-1 and can be developed to a biocontrol agent for biological control of the cucumber root-knot nematodes. Compared with an existing chemical agent for preventing cucumber root-knot nematodes, the purpureocillium lilacinum has the characteristics of being high in efficiency, low in toxicity, few in residues and free of pollution, and being unlikely to generate drug resistance. Besides strong virulence on the cucumber root-knot nematodes, the strain is quickly cultivated, the sporulation quantity is great, the germination rate of spores is high, and the strain is easy to prepare and has good market application prospects.

The invention relates to a Purpureocillium lilacinum strain with strong virulence to Botrytis cinerea and application thereof. The strain is Purpureocillium lilacinum pt362 and can be developed into a biocontrol bacterium preparation for biocontrol of Botrytis cinerea. Compared with conventional chemical agents for preventing and treating Botrytis cinerea, the strain provided by the invention has the characteristics of high efficiency, low toxicity, low residues, no pollution and small possibility of incurring drug resistance. The strain has strong virulence to Botrytis cinerea and is fast to culture, great in sporulation quantity, high in the germination rate of spores and easy to prepare; so the strain has good market application prospects.

The invention belongs to the technical field of agriculture and discloses a microbial agent for controlling root-knot nematode. The microbial agent is prepared from bacillus subtilis, purpureocillium lilacinum, bacillus cereus and acremonium strictum, wherein the living bacteria count ratio of the bacillus subtilis to the purpureocillium lilacinum to the bacillus cereus to the acremonium strictum is (1-50): (1-50): (1-50): (1-50); and the living bacteria count is greater than 200,000,000 / ml. In addition, the invention also provides a bacterial fertilizer for controlling the root-knot nematode, which comprises the microbial agent, a fertilizer and a carrier. The microbial agent is combined with the fertilizer, so that the nutrient supply for accelerating the plant growth can be improved when nematode is controlled.

The invention discloses a bio-control curing compound bactericide and a preparation method thereof. The bio-control curing compound bactericide is prepared by mixing 10 to 20 parts by weight of Purpureocillium lilacinum, 10 to 20 parts by weight of Pseudomonas fluorescens, 10 to 20 parts by weight of Bacillus cereus, 10 to 20 parts by weight of Bacillus mucilaginosus, 20 to 30 parts by weight of Bacillus subtilis and 10 to 20 parts of Trichoderma viride according to a certain ratio after being individually cultured. The bio-control curing compound bactericide provided by the invention has the advantages of preventing decline of a certain strain caused by culture after mixing, ensuring that the strains of the compound bactericide have different functions and perfectly matched, and having efficacies of fixing nitrogen, solubilizing phosphorus, dissolving potassium, decomposing organic matter, inhibiting pests, promoting organic matter conversion, accelerating the decomposition of organic matter and nutrients, turning waste into treasure, reducing pollution and the like. The product produce is a multi-functional compound bio-control bacterial group, has a good solution to the current soil improvement, agricultural environmental protection, and waste straw garbage, and is an ideal product capable of improving the soil, accelerating the growth of crops, and accelerating the decomposition of organic matter.

The invention relates to the technical field of microbial agents, and provides a compound microbial agent for biological control of nematodes and application of the compound microbial agent for biological control of nematodes in order to solve the technical problem that a good and stable nematode control effect cannot be achieved for a long time. The compound microbial agent for biological control of nematodes is composed of active bacteria and an adsorbent, wherein the adsorbent is a biomass-based porous material, and the biomass-based porous material is prepared from biodegradable resin, a porous carbon material, agricultural and sideline products and carbonate; and the active bacteria comprise purpureocillium lilacinum, beauveria bassiana and bacillus thuringiensis. According to the invention, carbon and nitrogen elements are provided for growth of plants in a release process, plant growth is promoted, the survival rate of plants is increased, and the effect of controlling nematodes in a persistent prevention and control manner can be achieved.

The invention discloses a preparation method of a composite biocontrol microbial agent. The preparation method comprises the following steps: preparing the following raw materials: purpureocillium lilacinum, beauveria bassiana, bacillus thuringiensis, modified starch, modified chitin, modified chitosan, azotobacter chroococcum and bacillus siamensis mixed fermentation liquor and potassium fulvate;respectively inoculating purpureocillium lilacinum, beauveria bassiana and bacillus thuringiensis into a seed culture medium, carrying out shaking culture to obtain a shake flask seed solution, and inoculating the shake flask seed solution into a sterilized fermentation tank submerged liquid fermentation culture medium to obtain a liquid fermentation product; fully stirring and uniformly mixing the liquid fermentation product with modified starch, modified chitin, the azotobacter chroococcum and bacillus siamensis mixed fermentation liquor and potassium fulvate to prepare a solid fermentationproduct; and conducting drying until the water content is 15-20%, and conducting crushing and sieving. The purpureocillium lilacinum, beauveria bassiana and bacillus thuringiensis are synergisticallymatched to achieve a good prevention and treatment effect on various plant diseases and insect pests.

The invention provides a microbial selenium-rich medical fertilizer for preventing and treating root-knot nematode disease, clubroot and soil insects. The microbial selenium-rich medical fertilizer comprises purpureocillium lilacinum, bacillus subtilis, trichoderma harzianum, total matrine, abamectin, selenium-enriched yeast, sugar alcohol composite boron, calcium fulvate, amino acid, tea seed cake, cane sugar and turf. The microbial selenium-rich medical fertilizer is prepared from the following steps: uniformly mixing the tea seed cakes and the turf added with 50% of water, uniformly mixingthe mixture with the selenium-enriched yeast and the cane sugar added with 10% of water, stacking and carrying out aerobic fermentation to form a mixture A; turning and crushing the mixture A, sequentially adding the sugar alcohol composite boron, the total matrine, amino acid raw powder and the calcium fulvate, and fully and uniformly stirring to form a mixture B; and adding the bacillus subtilis, the purpureocillium lilacinum, the trichoderma harzianum and the abamectin into the mixture B, and fully and uniformly stirring to obtain the selenium-rich medical fertilizer. The microbial selenium-rich medical fertilizer can prevent and treat the root-knot nematode disease, clubroot and soil insects. The selenium-rich microbial insecticide-fertilizer is suitable for preventing and treating theroot-knot nematode disease, clubroot and soil insects.

The invention relates to a purpureocillium lilacinum-lactobacillus plantarum composite microbe agent. The composite microbe agent comprises a purpureocillium lilacinum microbe agent and a lactobacillus plantarum microbe agent in a weight ratio of 8-12:1. The spore concentration of the purpureocillium lilacinum microbe agent is not less than 10<9> spore / mL. The spore concentration of the lactobacillus plantarum microbe agent is not less than 15.0*10<9> cfu / mL. Through combination of the purpureocillium lilacinum and the lactobacillus plantarum, soil-borne pathogens can be inhibited, and disease resistance of plants can be improved so as to reduce harm to vegetables, thus promoting vegetable growth and reducing harms caused by root-knot nematode.

The invention relates to a purpureocillium lilacinum strain and application thereof in prevention and treatment of phyllotreta striolata, and belongs to the technical field of biopesticides. The purpureocillium lilacinum PlSC59A3 strain is already preserved in the guangdong microbial culture collection center, the preservation date is 06 November 2020, and the preservation number is GDMCC 61232. The purpureocillium lilacinum PlSC59A3 strain is efficient in prevention and treatment of phyllotreta striolata, is an entomopathogenic fungus, is used as a biopesticide, has the characteristics of nopollution to the environment, difficulty in generation of drug resistance of pests and no toxicity to human and livestock, and is suitable for production requirements of green healthy agricultural products.

The invention discloses a plant root-knot nematode biocontrol bacterium as well as a preparation and an application thereof. The purpureocillium lilacinum CCTCC NO.M2016682 screened through research has strong pathogenicity on plant root-knot nematodes, and can be used as a plant root-knot nematode biocontrol bacterium; a plant root-knot nematode biocontrol agent containing the purpureocillium lilacinum, conidia of the purpureocillium lilacinum, metabolites of the purpureocillium lilacinum or solid fermentation products of the purpureocillium lilacinum can be prepared; the plant root-knot nematode biocontrol agent is applied to soil in a soil mixing form, so that plant root-knot nematode can be effectively prevented and treated; the high-yield and high-quality leucinostatin can be extracted or prepared by using the purpureocillium lilacinum. The biocontrol bacterium and the biological agent are low in production cost, easy to prepare, efficient, low in toxicity, environmentally friendly, free of pollution and not prone to generating drug resistance; the effects of environmental protection, safety, low cost and high-efficiency prevention and treatment of plant root-knot nematodes can be achieved.

The invention provides two Purpureocillium lilacinum genes Pth11-rg1 and Pth11-rg2 which are homologous with a rice blast transmembrane protein coding gene pth11, the Pth11 gene is subjected to function shielding by utilizing a gene knockout technology to obtain deltaPth11-rg1 and deltaPth11-rg2 gene engineering strains, and the functions of the Pth11-rg1 and Pth11-rg2 genes of the Purpureocillium lilacinum are verified by utilizing the gene engineering strains. Therefore, primary identification of functions on the gene level can be efficiently carried out.

The invention discloses purpureocillium lilacinum and application thereof in chicken feather degradation. The purpureocillium lilacinum is separated from soil in Kunming City, Yunnan Province. The strain is GZAC18-2JMP, and is preserved in the China General Microbiological Culture Collection Center on August 27, 2020, and the preservation number is CGMCC No. 20249. Researches show that the purpureocillium lilacinum is medium-temperature bacteria and can degrade chicken feathers, the optimal culture method for degrading the chicken feathers comprises the following steps of inoculating the GZAC18-2JMP strain into basic fermentation liquor added with the chicken feathers, standing for 48 hours at the temperature of 25 DEG C, and carrying out shaking culture for 96 hours at the speed of 110rpm / min, wherein the chicken feathers in the fermentation liquor are crushed, the soluble protein is 175.7 mu g / mL, and the dry weight of chicken feather filter residues is 0.36 g; and the chicken feather degradation rate is 64%. The strain and the chicken feather degradation culture method thereof can be used for biodegradation of chicken feathers.

The invention provides a fermenting medium for producing spores by liquid submerged fermentation of Purpureocillium lilacinum and a production method of the fermenting medium. The fermenting medium comprises 45-80 g / L of sucrose or glucose, 23-40 g / L of soluble starch, 20-35 g / L of soybean flour or cottonseed powder, 2-4 g / L of monopotassium phosphate, 0.1-0.4 g / L of anhydrous calcium chloride, 0.1-0.3 g / L of magnesium sulfate heptahydrate, 0.01-0.037 g / L of cobalt chloride hexahydrate, 0.01-0.05 g / L of iron sulfate heptahydrate, 0.005-0.016 g / L of manganese sulfate monohydrate, and 0.005-0.014 g / L of zinc sulfate heptahydrate. Fermenting time of the liquid submerged fermentation is 55-65 h; fermenting temperature is 26-28 DEG C; dissolved oxygen control is subjected to two-stage control;dissolved oxygen value is controlled to 45-60% in the 0-24-hour bacterial growth stage of 0-24 hours, and the dissolved oxygen value is controlled to 10-15% in the 24-65-hour spore formation and maturing stage. The fermenting cycle for producing spores by liquid submerged fermentation of Purpureocillium lilacinum is shortened evidently; spore yield is increased greatly; fermentation cost of Purpureocillium lilacinum is reduced; the produced spores can well kill root-knot nematodes.

The invention discloses a composite microbial soil remediation agent and a preparation method thereof. The composite microbial soil remediation agent is prepared from the following raw materials in parts by weight: 10-18 parts of a composite microbial agent, 30-70 parts of organic matters and 10-25 parts of inorganic raw materials, the compound microorganisms are prepared from the following microorganisms in parts by weight: 0.5 to 5 parts of bacillus subtilis, 0.5 to 5 parts of bacillus licheniformis, 0.3 to 4 parts of bacillus mucilaginosus, 0.2 to 3 parts of bacillus megaterium and 0.2 to 3 parts of purpureocillium lilacinum; the organic matter is crop waste; the inorganic raw material is a mixture of sepiolite, medical stone and apatite; the compound microorganism soil remediation agent has a good heavy metal contaminated soil remediation effect and has a remarkable effect in the aspects of improving the physical and chemical properties of soil and remediating soil pollution, the remediation rate of the heavy metal contaminated soil reaches 50% or above, and the yield of crops can be remarkably increased.

The invention relates to purpureocillium lilacinum with high pathogenicity to oilseed rape sclerotinia sclerotiorum and application thereof. The strain is purpureocillium lilacinum pt361, and can be developed into a fungi-proofing preparation to be used for biological control of sclerotinia rot of colza. Compared with an existing chemical agent for controlling oilseed rape sclerotinia sclerotiorum, the strain has the advantages of being efficient, low in toxicity, low in residue amount, free of pollution and capable of reducing the possibility of drug resistance. Besides high pathogenicity to oilseed rape sclerotinia sclerotiorum, the strain is also characterized in high culture speed, large sporulation quantity, high spore germination rate, easy preparation and great market application prospects.

The present invention relates to a seedling culture matrix as a healing promoting agent for promoting quick healing of a cucumber grafting wound and an application thereof. A matrix formula comprisesgrass carbon, perlite and vermiculite with the ratio of 6:3:1, and 0.5% of brown sugar, 0.2% of potassium dihydrogen phosphate and 1% of a purpureocillium lilacinum agent (pt361 and number: CCTCCM2016681) are added, so that the matrix can be developed into the healing promoting agent for promoting quick healing of the cucumber grafting wound; and compared with different healing promoting agents atpresent, the healing promoting agent has the characteristics of shortening quick healing time of the wound, preventing diseases and strengthening seedlings. The strain disclosed by the invention notonly promotes quick healing of the cucumber grafting wound, but also improves the seed germination rate, promotes the growth and shortens the commercial seedling period.

The invention provides an isolated Purpureocillium lilacinum strain, a composition containing the same, and an application of the strain in prevention and treatment of Meloidogyne. The strain is highin sporulation capacity, high in viable count and good in prevention and treatment effect, and can be used for prevention and treatment of various plants.

The invention relates to the technical field of microbial control, in particular to a microbial agent for controlling zingiber officinale roscoe and application of the microbial agent. The microbial agent is prepared from the following raw materials in parts by weight: 3 to 8 parts of purpureocillium lilacinum, 3 to 8 parts of trichoderma harzianum, 8 to 12 parts of enzymolysis amino acid polypeptide powder, 45 to 55 parts of glucose, 3 to 8 parts of calcium hydroxide, 10 to 15 parts of monopotassium phosphate and 10 to 15 parts of dipotassium phosphate. According to the microbial agent disclosed by the invention, the purpureocillium lilacinum is matched with the trichoderma harzianum, and meanwhile, nematode thalli and eggs are killed in a parasitic manner, so that the effect of preventing and treating the zingiber officinale nematode disease is achieved.

The invention discloses paecilomyces lilacinus and application of the paecilomyces lilacinus in inhibiting plant growth. The paecilomyces lilacinus is named as Purpureocillium lilacinum Scaumcx04. Thepreservation number is GDMCC NO: 60794, and the strain is preserved in Guangdong Province Microbial Culture Collection Center on September 27, 2019, wherein the Guangdong Province Microbial Culture Collection Center is located in the 5th floor of the building No. 59 of the courtyard No.100 of Xianlie Middle Road of Guangzhou City. A culture obtained by culturing the paecilomyces lilacinus or spores of the paecilomyces lilacinus and secondary metabolite which is obtained by further organic solvent extraction and chromatographic column purification can inhibit growth of stems on the ground of plants and growth of roots under the ground of the plants, meanwhile, plant seed germination can be inhibited, and the germination time is prolonged, so that the paecilomyces lilacinus can be used as aregulator for plant growth and a plant herbicide, and an effective way is provided for development of plant growth regulators and herbicides.

The invention discloses purpureocillium lilacinum and application thereof. The strain is purpureocillium lilacinum BPT05 and was preserved in Guangdong Microbiological Culture Collection Center (GDMCC) of Guangdong Institute of Microbiology, Floor 5, Building 59#, 100#, Xianlie Middle Road, Guangzhou City, and the preservation number is GDMCC NO: 60178. The strain generates substances with the effect of promoting plant growth, in particularly with the effect of remarkably promoting the growth of tobacco. The strain is used for performing liquid fermentation and the fermentation products are subjected to aftertreatment, so that the biological agent for promoting the growth of the tobacco can be prepared; the biological agent for promoting the growth of the tobacco is convenient to storage and transport, convenient to use and stable in activity, and can effectively improve the agronomic trait of the tobacco and increase the yield of the tobacco; and the biological agent for promoting the growth of the tobacco is environment-friendly and pollution-free to soil and environment, and has important significance in agricultural sustainable development.

The invention discloses a composite microbial agent containing streptomycete, which is characterized in that the composite microbial agent comprises streptomycete, purpureocillium lilacinum and trichoderma harzianum; the streptomyces comprises streptomyces microflavus, streptomyces dense and streptomyces rochei; the weight ratio of the streptomyces to the purpureocillium lilacinum to the trichoderma harzianum is (4-6): (1-3): 1; according to the compound microbial agent prepared by the invention, various strains are reasonably proportioned and have a synergistic effect, so that gray mold and leaf mold of crops can be efficiently prevented and treated; meanwhile, crop growth can be stimulated, and crop yield and quality are improved.

The invention relates to the technical field of agriculture, in particular to preparation and application of a Purpureocillium lilacinum biological seedling substrate. A functional flora taking a purpureocillium lilacinum strain Pl36-1-1 as a main microorganism is formed in a substrate, so that the healthy growth of plants is protected from a seedling raising stage, and the potential of the microorganism is comprehensively exerted; and the prevention and control effect of microbial products on vegetable soil spread diseases is improved. The method comprises the following steps: S1, adding 10-20 parts of cane sugar, 1-6 parts of trace elements and 15-30 parts of rice husks into 100-150 parts of agricultural wastes, performing uniform stirring, performing fermenting, performing decomposing, performing sieving, adding perlite and vermiculite, and performing uniformly mixing to obtain a primary fermentation product; and S2, mixing 10-20 parts of cane sugar into the primary fermentation product, performing uniform stirring, inoculating Purpureocillium lilacinum Pl36-1-1 strain conidia powder, and performing uniform mixing to obtain the Purpureocillium lilacinum seedling culture substrate.

The invention relates to the field of biopesticides, and particularly discloses a microorganism-plant two-way insect-resistant fungicide and a preparation method thereof. The insect-resistant fungicide is prepared from, by weight, 5-10 parts of bacillus thuringiensis liquid, 5-10 parts of beauveria bassiana liquid, 10-15 parts of purpureocillium lilacinum liquid and 70-80 parts of plant extract liquid. The preparation method comprises the following steps: weighing the bacillus thuringiensis liquid, the beauveria bassiana liquid, the purpureocillium lilacinus liquid and the plant extract liquid, mixing and stirring to prepare the insect-resistant fungicide. The insect-resistant fungicide has the advantages of being good in compounding effect, stable in mixing, wide in antibacterial spectrum, safe and harmless to human and livestock, and easy to degrade.

The invention relates to a P. lilacensis strain and its application in preventing and treating flea beetle, and belongs to the technical field of biological pesticides. The Purpureocillium lilacinum PlSC59A3 strain has been preserved in the Guangdong Microbial Culture Collection Center on October 16, 2020, and the preservation number is GDMCC No: 61232. The PlSC59A3 strain of P. lilacinus provided by the present invention is highly effective in preventing and treating flea beetle. The strain is an entomopathogenic fungus. As a biological pesticide, it has no pollution to the environment, is not easy for pests to develop drug resistance, and is harmless to humans and animals. It is suitable for the production needs of green and healthy agricultural products.

The invention relates to a fermentation culture medium for increasing leucinostatin produced from purpureocillium lilacinum. The fermentation culture medium comprises, by weight, 45-55 parts of glucose, 4.5-5.5 parts of peptone, 4.5-5.5 parts of yeast extract, 0.5-2.0 parts of K2HPO4, 8.5-12.5 parts of Na2CO3, 0.1-0.5 part of MgSO4.7H2O, 1.5-10 parts of corn flour, 0.005-0.05 part of FeSO4.7H2O and 1.5-10.5 parts of urea. A culture method for increasing the leucinostatin produced from the purpureocillium lilacinum by the aid of the fermentation culture medium includes steps of carrying out fermentation culture on culture bacterium solution by the aid of purpureocillium lilacinum shake flasks; carrying out culture on the culture bacterium solution in seed tanks by the aid of the fermentation culture medium; inoculating the culture bacterium solution in the seed tanks into fermentation tanks and carrying out fermentation. The fermentation culture medium and the culture method have the advantages that the purpureocillium lilacinum cultured by the aid of the fermentation culture medium is low in cost, short in fermentation cycle and high in leucinostatin yield, and the like.

The invention belongs to the field of pesticides, and particularly relates to a pesticide for preventing and treating pepper root-knot nematode, which is prepared from the following materials in parts by weight: 5 to 10 parts of clothianidin, 10 to 15 parts of beauveria bassiana, 5 to 10 parts of purpureocillium lilacinum and 20 to 30 parts of trichoderma longibrachiatum. The auxiliary agent comprises the following components in parts by weight: 3-5 parts of pesticide emulsifier 700 #, 2-6 parts of palygorskite, 3-5 parts of polyester resin, 1-2 parts of citric acid, 3 parts of ink black, 30-50 parts of starch, 7-9 parts of propylene glycol and 1-2 parts of an odor agent. According to the invention, Beauveria bassiana and Purpureocillium lilacinus are added to improve the control effect on root-knot nematodes, Trichoderma longibrachiatum improves and keeps the continuous control effect on root-knot nematode eggs in soil, the root-knot nematode eggs are prevented from relapsing after being killed, and the product can be prevented from being eaten by mistake by adding ink black and an odorizor.

The invention discloses a strain of P. lilacinus and its application. The strain is Purpureocillium lilacinum (Purpureocillium lilacinum) BPT05, which was preserved on May 09, 2017 in the Guangdong Provincial Microbiological Culture Collection Center on the 5th Floor, Building 59, Compound 59, No. 100 Xianlie Middle Road, Guangzhou City. Deposit number: GDMCC NO: 60178. During the process of growth and metabolism, the bacterial strain produces substances that can promote plant growth, especially significantly promote the growth of tobacco. Using the strain for liquid fermentation, and then post-processing the fermentation product, it can be prepared into a tobacco growth-promoting biological bacterial agent. The tobacco growth-promoting biological bacterial agent is convenient for storage and transportation, easy to use, stable in activity, and can effectively improve the agronomic properties of tobacco. It can increase tobacco production; it is environmentally friendly and has no pollution to soil and environment, which is of great significance to the sustainable development of agriculture.