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Preparation method and application of composite biocontrol microbial agent

A technology for biocontrol microorganisms and inoculants, which is applied in the field of preparation of compound biocontrol microbial inoculants, can solve problems such as high drug resistance of pathogenic microorganisms, affecting the quality of agricultural products, health, and ecological environment.

Inactive Publication Date: 2021-03-23
中诚国联(河南)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, at present, most of the prevention and control of plant diseases still rely on chemical agents for prevention and control, which has an impact on the ecological environment. The resistance of pathogenic microorganisms is increasing, and it also seriously affects the quality of agricultural products and the health of the people. Therefore, it is urgent to prepare a A compound biocontrol microbial agent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Proportional preparation includes the following raw materials in parts by weight: 20 parts of Porphyra lilacinus, 15 parts of Beauveria bassiana, 10 parts of Bacillus thuringiensis, 6 parts of modified starch, 2 parts of modified chitin, and modified chitosan 1 part, 10 parts of mixed fermented liquid of nitrogen-fixing bacterium Ziem bacillus, 1 part of potassium fulvate; inoculate the seed culture medium with Porphyra lilacinus, Beauveria bassiana, and Bacillus thuringiensis respectively, and carry out shaker culture , to obtain the shake flask seed solution, inoculate the shake flask seed solution in the submerged liquid fermentation medium of the sterilized fermenter, carry out fermentation culture, and obtain the liquid fermentation product; the liquid fermentation product is mixed with modified starch and modified chitin , mixed fermented liquid of nitrogen-fixing bacteria Bacillus cymum and potassium fulvic acid were fully stirred, mixed evenly, fermented for 7 da...

Embodiment 2

[0027] Proportional preparation includes the following raw materials in parts by weight: 22 parts of Porphyra lilacinus, 17 parts of Beauveria bassiana, 12 parts of Bacillus thuringiensis, 7 parts of modified starch, 2.5 parts of modified chitin, and modified chitosan 1.5 parts, 12 parts of mixed fermentation broth of Azotobacter rufosa-Bacillus sime, and 1.5 parts of potassium fulvate; inoculate P. lilacinus, Beauveria bassiana, and Bacillus thuringiensis in the seed medium respectively, and carry out shaking table Cultivate to obtain the shake flask seed liquid, inoculate the shake flask seed liquid into the submerged liquid fermentation medium of the sterilized fermenter, carry out fermentation culture, and obtain the liquid fermentation product; mix the liquid fermentation product with modified starch, modified chitin quality, Azotobacter rufosa-Sim bacillus mixed fermentation liquid, potassium fulvic acid were fully stirred, evenly mixed, fermented for 8 days, and made int...

Embodiment 3

[0033] Proportional preparation includes the following raw materials in parts by weight: 25 parts of Porphyra lilacinus, 20 parts of Beauveria bassiana, 15 parts of Bacillus thuringiensis, 8 parts of modified starch, 3 parts of modified chitin, and modified chitosan 2 parts, 13 parts of mixed fermented liquid of Azotobacter rufosa-Bacillus sime, 2 parts of potassium fulvate; inoculate P. lilacinus, Beauveria bassiana, and Bacillus thuringiensis in the seed medium respectively, and carry out shaking table Cultivate to obtain the shake flask seed liquid, inoculate the shake flask seed liquid into the submerged liquid fermentation medium of the sterilized fermenter, carry out fermentation culture, and obtain the liquid fermentation product; mix the liquid fermentation product with modified starch, modified chitin quality, Azotobacter rufosa-Sim bacillus mixed fermentation liquid, potassium fulvic acid were fully stirred, mixed evenly, and fermented for 9 days to make a solid ferme...

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Abstract

The invention discloses a preparation method of a composite biocontrol microbial agent. The preparation method comprises the following steps: preparing the following raw materials: purpureocillium lilacinum, beauveria bassiana, bacillus thuringiensis, modified starch, modified chitin, modified chitosan, azotobacter chroococcum and bacillus siamensis mixed fermentation liquor and potassium fulvate;respectively inoculating purpureocillium lilacinum, beauveria bassiana and bacillus thuringiensis into a seed culture medium, carrying out shaking culture to obtain a shake flask seed solution, and inoculating the shake flask seed solution into a sterilized fermentation tank submerged liquid fermentation culture medium to obtain a liquid fermentation product; fully stirring and uniformly mixing the liquid fermentation product with modified starch, modified chitin, the azotobacter chroococcum and bacillus siamensis mixed fermentation liquor and potassium fulvate to prepare a solid fermentationproduct; and conducting drying until the water content is 15-20%, and conducting crushing and sieving. The purpureocillium lilacinum, beauveria bassiana and bacillus thuringiensis are synergisticallymatched to achieve a good prevention and treatment effect on various plant diseases and insect pests.

Description

technical field [0001] The invention relates to the technical field of microbial bacterial agents, in particular to a preparation method of a composite biocontrol microbial bacterial agent. Background technique [0002] Biocontrol is biological control, which refers to the method of using one organism to deal with another organism. Biological control can be roughly divided into three categories: controlling pests by insects, controlling pests by birds and controlling pests by bacteria. It is a method of reducing the population density of harmful organisms such as weeds and pests. It takes advantage of the interrelationships between biological species to suppress another or another type of organisms with one or one type of organisms. Its biggest advantage is that it does not pollute the environment, which is incomparable to non-biological pest control methods such as pesticides. [0003] However, at present, most of the prevention and control of plant diseases still rely o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/60C05G3/80C05F17/20C12N1/20C12N1/14C12R1/07C12R1/645C12R1/79C12R1/10C12R1/085C12R1/11C12R1/065C12R1/01
CPCC05G3/00C05G3/60C05G3/80C05F17/20C05F11/00C12N1/20C12N1/14C05F11/02C05F11/08Y02W30/40
Inventor 祝春华朱永杰石朋飞孙辉
Owner 中诚国联(河南)生物科技有限公司
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