39 results about "Aureobasidium sp." patented technology

The invention relates to the field of agricultural microbial preparations, in particular to a complex microbial inoculant sustained-release granule for preventing and treating sweet potato stem nematode disease and a preparation method of the complex microbial inoculant sustained-release granule. Comprising the following components in parts by weight: 15-25 parts of paecilomyces lilacinus viable bacterium powder, 10-20 parts of bacillus thuringiensis viable bacterium powder, 10-20 parts of streptomyces viable bacterium powder, 5-15 parts of bacillus subtilis viable bacterium powder, 15-25 parts of charcoal, 5-15 parts of sodium alginate, 5-15 parts of humic acid, 5-10 parts of chitosan, 3-8 parts of calcium silicate, 1-5 parts of mannitol and 1-5 parts of sodium lignin sulfonate. Four microorganisms with complementary functions, namely paecilomyces lilacinus, bacillus thuringiensis, streptomycete and bacillus subtilis, are adopted, the sweet potato stem nematode disease is synergistically controlled through multiple action mechanisms, and the control effect and the stability are remarkably improved.

The invention provides a soil conditioner for improving soil hardening and a preparation method thereof, and relates to the technical field of soil conditioning. The soil conditioner is prepared by combining nano silicon dioxide, a straw retting material, a straw composite fermentation material, biochar, a water-retaining agent and modified starch. The straw composite fermented material is prepared by the following steps: crushing straws, mixing with sweet water, stirring, drying, carrying out microwave treatment, mixing with bacillus thuringiensis, fermenting, sterilizing, subsequently adding a composite microbial agent of bacillus subtilis, trichoderma and bacillus mucilaginosus, continuously fermenting, and drying. The modified starch is prepared by semi-gelatinizing corn starch and then carrying out microwave drying and shaping. According to the present invention, the defects in the prior art are overcome, the soil hardening phenomenon can be rapidly improved with the small addition amount, the soil porosity is increased, the activity and the diversity of the soil microorganisms are ensured, and the soil fertility is improved.

The invention provides a bacillus thuringiensis strain, a fermentation culture method and application of the bacillus thuringiensis strain. The strain is preserved in the China Center for Type Culture Collection on September 28, 2011, the preservation address is No. 299 on eight road, Wuchang District, Wuhan City, Hubei Province, the preservation number is CCTCC No: M 2011334, and the preservation date is September 28, 2011. The strain cultured by the fermentation method has efficient, specific and stable insecticidal activity on culex, aedes and other mosquitoes, has the advantages of low preparation cost, environmental friendliness and the like, can be developed into a biological insecticide for preventing and killing mosquitoes and preventing and controlling malaria, dengue fever, chikungunya and other mosquito-borne infectious diseases, and meets the requirements of green and safe prevention and control in China.

The invention belongs to the technical field of environmental engineering, and discloses a microbial agent for degrading malathion as well as a preparation method and application of the microbial agent. The microbial agent comprises the following raw materials: compound bacteria, a nutritional supplement and a biomass carrier material, wherein the compound bacteria comprise functional bacteria and synergistic bacteria, and the mass ratio of the functional bacteria to the synergistic bacteria is (1.2-1.8): 1; the functional bacteria comprise issatchenkia orientalis, nocardia, brevibacillus laterosporus and bacillus cereus; the synergistic bacteria comprise azospirillum brasilense, bacillus thuringiensis and lactobacillus buchneri. Functional bacteria with direct degradation capacity and synergistic bacteria with auxiliary functions are creatively compounded according to a specific proportion, meanwhile, the garlic straw powder is selected as a biomass carrier material, all the raw materials complement each other in function and have a synergistic effect, the degradation rate and thoroughness of malathion are remarkably improved, and the degradation efficiency of malathion is greatly improved. The defects of low degradation efficiency and poor environmental adaptability of a single strain are overcome.

The invention discloses a bacillus thuringiensis CMS-BT17, which is classified and named as bacillus thuringiensis, is preserved in the China General Microbiological Culture Collection Center (CGMCC), has the preservation number of CGMCC 35313, and is preserved on July 21, 2025. The bacillus thuringiensis strain CMS-BT17 directly separated and screened from a zizania aquatica soil sample in Haikou City of Hainan carries four insecticidal protein coding genes of Cry8Ca, Cry8Da, Spp1Aa and Vpb4Ca, not only is high in insecticidal toxicity and good in insecticidal effect, but also has a certain effect of inhibiting tomato fungal diseases, can be widely applied to prevention and control of agricultural diseases and pests, and has a wide application prospect. The sustainable development of green agricultural production is promoted.

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

The present invention describes capsule compositions consisting of crosslinked chitosan, beta-glucan or alginate, and encapsulates an environmentally compatible ingestible larvicide, especially larvicidal agents such as essential oils or other ingestible larvicides such as Bacillus thuringiensis which are lethal to the larvae of pest insects, especially mosquito larvae.

This application relates to the field of microbial strain technology, specifically disclosing a Bacillus thuringiensis HP-SYJ2 strain and its applications. The Bacillus thuringiensis HP-SYJ2 strain is deposited at the China Center for Type Culture Collection (CCTCC), accession number: CCTCC NO: M2025343, deposit date: March 3, 2025, address: Wuhan University, Wuhan, China. The strain of this application can be used to prepare insecticides for controlling underground pests, which is of great significance for the biological control of plant diseases and pests.

The invention discloses a carbon-based biological liquid organic water-soluble fertilizer as well as a preparation method and application thereof, and belongs to the technical field of biological organic fertilizers. The water-soluble fertilizer comprises the following raw materials in parts by weight: 15-25 parts of a seaweed extract, 10-15 parts of potassium fulvate, 5-10 parts of a compound microbial agent, 3-5 parts of potassium dihydrogen phosphate, 2-4 parts of potassium nitrate, 2-3 parts of urea, 2-3 parts of calcium nitrate, 1-2 parts of magnesium sulfate heptahydrate, 1-1.5 parts of a trace element-containing substance, 1-2 parts of sodium dodecyl benzene sulfonate and 0.5-1 part of potassium sorbate. The total amount of the components is 100 parts. According to the invention, three strains such as pseudomonas sevivaldi, bacillus pumila and bacillus thuringiensis are screened to play roles in three dimensions of promoting growth, improving rhizosphere environment and preventing and treating diseases and insect pests respectively, and are combined with organic components such as seaweed extract and potassium fulvate in the fertilizer and other components, so that the fertilizer has a good application prospect. A comprehensive system of nutrition, growth promotion, disease resistance and quality improvement is formed, and the yield and quality of grapes are remarkably improved.

The invention discloses a microbial preparation for preventing and treating plant root-knot nematode as well as a preparation method and application thereof, and belongs to the technical field of microbial preparations. The microbial preparation for preventing and treating the plant root-knot nematode is of a double-layer coating structure and comprises an inner layer and an outer layer, the inner layer comprises phaffia rhodozyma fermentation liquor, paecilomyces lilacinus fermentation liquor, bacillus subtilis fermentation liquor, bacillus thuringiensis fermentation liquor, beauveria bassiana fermentation liquor and an inner layer carrier material; the outer layer comprises a streptomyces densely-rotating fermentation liquid and an outer layer carrier material; according to the application, the phaffia rhodozyma, the paecilomyces lilacinus, the bacillus subtilis, the bacillus thuringiensis fermentation liquor, the beauveria bassiana fermentation liquor and the streptomyces dense are compounded, and the strains have a synergistic effect, so that functional complementation is realized; a double-layer coating structure is adapted to acid soil, guarantees the initial activity of the strain and improves the survival efficiency of the strain on one hand, and can prolong the shelf life and the function exerting period on the other hand, so that long-acting pest control is realized.

The present invention provides a planting method for promoting the early survival and growth of Panax notoginseng, and relates to the technical field of Panax notoginseng planting. The planting method mainly comprises the following steps: disinfecting and hormone-treating the Panax notoginseng seeds in the early stage, fumigating them with a certain concentration of cymene, and then watering them with a sterilized solution of pine needles and corn fermented with Bacillus thuringiensis after sowing. The present invention overcomes the shortcomings of the existing technology, can effectively improve the early emergence efficiency of Panax notoginseng, ensure uniform emergence of seedlings, promote root growth of Panax notoginseng seedlings, increase the vigorous seedling rate of Panax notoginseng seedlings, and comprehensively improve the economic benefits of Panax notoginseng planting.

This invention discloses a method for the domestication, isolation, purification, and application of a low-temperature resistant straw-degrading bacterium, belonging to the fields of agricultural microbiology technology and waste resource utilization. The domestication method specifically includes sample preparation, straw pretreatment, culture medium preparation, and multiple rounds of low-temperature domestication culture. During this process, the straw weight loss rate is used as an indicator to detect the degradation efficiency of the bacterial community after each round of domestication. The obtained low-temperature resistant straw-degrading bacterium was deposited on April 27, 2026, at the China Center for Type Culture Collection (CCTCC), located at No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province; the accession number is CCTCC NO: M2026814; the taxonomic name of the low-temperature resistant straw-degrading bacterium is *Brucea buddinga* X4-8. (Aureobasidium sp. X4-8) The domestication method of this invention actively enhances the low-temperature adaptability and degradation activity of microbial communities by combining exogenous amino acid induction with systematic cyclic domestication. Furthermore, this invention also discloses a method for isolating and purifying low-temperature resistant straw-degrading bacteria and its application.

The invention discloses a hydrogel and probiotic compounded microbial inoculum composition and a vegetable planting matrix, a three-dimensional gel carrier is used for embedding a microbial inoculum, the carrier integrates mechanical properties and thallus protection and can maintain flora activity for a long time, bacillus thuringiensis has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression, and the microbial inoculum composition has the advantages that the microbial inoculum composition has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression; the microbial agent composition can survive for a long time in a low-oxygen environment of a carrier embedding environment, and is cooperated with bacillus cereus, azotobacter chroococcum and bacillus megatherium to promote the growth of plants such as vegetables and the like and directionally kill pathogenic microorganisms such as root-knot nematode. Corresponding pests can be killed in a green and targeted manner, the growth of plants is promoted, and the yield is increased.

The invention discloses bacillus thuringiensis CMS-5E8, which is classified and named as bacillus thuringiensis, and is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, the preservation number is CGMCC 35311, and the preservation date is July 21, 2025. The strain carries 16 insecticidal protein coding genes, namely Cry1Aa, Cry1Ah, Cry1Be, Cry1If, Cry1Na, Cry1Ea, Cry1La, Cry2Ab, Cry2Ah, Vip3Aa, Vip3Ad, Spp1Aa, Vpb4Ca, Tpp78Ba, Mpp46Ab and Mpp3Aa, not only is high in insecticidal toxicity and good in insecticidal effect, but also has a certain effect of inhibiting fungal diseases, and can be widely applied to prevention and treatment of agricultural diseases and insect pests.

The invention relates to bacillus thuringiensis ZKSW003 as well as a fungicide and application thereof, and belongs to the technical field of microorganisms. The bacillus thuringiensis ZKSW003 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36074, the preservation date is September 25, 2025, and the address of the preservation institution is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention further provides application of the bacillus thuringiensis ZKSW003 in inhibition of harmful bacteria in soil, prevention and treatment of root-knot nematode and promotion of plant growth. The bacillus thuringiensis ZKSW003 disclosed by the invention not only can promote the growth of crops and realize weight loss and efficiency improvement, but also can control the occurrence of soil-borne fungus and bacterial diseases, and can inhibit the growth of root-knot nematode. Chemical pesticide application can be reduced, pesticide residues are reduced, and sustainable development of agricultural economy is promoted.

The invention relates to bacillus thuringiensis KHWY-1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.33400. The strain is used for fermenting feathers for 20 hours under the conditions that the temperature is 25 DEG C and the pH value is 8, the soluble protein content reaches 691.37 mu g / mL, and the feather degradation rate reaches 90% or above. The invention also relates to a new application of the bacillus thuringiensis KHWY-1 in degrading the feather fermentation filtrate of the white feather chicken as a cosmetic raw material, and the additional value of feather degradation products is improved.

The application discloses a saline-alkali soil special rice bio-organic fertilizer, which comprises halomonas, bacillus subtilis, paenibacillus polymyxa, bacillus thuringiensis, lactobacillus plantarum, longovis rubescens and organic fertilizer. In actual preparation, the microorganism is expanded to obtain a microbial agent, then the rotten cow dung and auxiliary materials are uniformly mixed, granulation drying is carried out, and after cooling treatment, the microbial agent and a coating agent mixed solution are added to carry out coating treatment, and then packaging is carried out. After the finished product is packaged, it can be used after being stored in a warehouse for a period of time. The microbial fertilizer has high organic matter content and active bacteria quantity, and in combination with conventional irrigation and fertilization measures, can improve the soil structure of saline-alkali soil, reduce the soil salt content and pH, enhance the stress resistance of crops, promote the growth of rice, and increase the yield, so that the effects of improving soil, fertilizing, resisting stress and promoting growth are achieved.

The invention discloses a compound microbial agent rich in beta-glucan as well as a preparation method and application of the compound microbial agent, and belongs to the technical field of agricultural biology. According to the microbial agent, an aureobasidium pullulans-rhizobium pullulans-bacillus subtilis-bacillus thuringiensis quaternary synergistic system is constructed, specific auxiliary materials, carriers and a coating technology are supplemented, and under the drought stress condition, the microbial agent can remarkably promote the growth (plant height and biomass) of the pakchoi, so that the yield of the pakchoi is increased, and the yield of the pakchoi is increased. The yield of the pakchoi is increased, the quality is improved (the content of soluble sugar and protein is increased), the chlorophyll content and the photosynthetic efficiency are improved, the antioxidant enzyme activity is enhanced, cell membrane damage is reduced, the physical and chemical properties of soil are improved, and a new effective solution is provided for agricultural green drought resistance.

The invention belongs to the field of gene engineering, and particularly relates to a bacillus thuringiensis binary toxin gene and application thereof in cotton bollworm prevention and control. The Cry-Tpp binary toxin combination with inhibitory activity on lepidoptera insects is identified for the first time, gene resources with commercial potential are provided for development of novel microbial insecticides and insect-resistant transgenic crops, and a new direction is provided for enhancing the activity of the Cry toxoid, artificially modifying the Cry toxoid and solving the problem of insect resistance. The method is of great significance to deep development and development innovation of Bt resources.

The present invention provides a multifunctional Bacillus thuringiensis LT2016 and its application, belonging to the technical field of biocontrol bacteria. The Bacillus thuringiensis LT2016 of the present invention is deposited in the General Microbiology Center of the China Microorganism Culture Collection Committee with a deposit number of CGMCC No. 28725 and a deposit date of October 24, 2023. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. The Bacillus thuringiensis LT2016 of the present invention has three functions: preventing and controlling lepidopteran pests such as diamondback moth, inhibiting fungal growth, and promoting corn growth and increasing yield. The microbial agent prepared using the biocontrol bacteria can reduce the amount of other chemical pesticides, reduce the cost of corn disease prevention and control, promote corn growth, and has good promotion value.

According to the bacillus thuringiensis and the application thereof in preventing and treating the plutella xylostella, the bacillus thuringiensis with specific activity on the plutella xylostella is screened, the types of insecticidal proteins of the bacillus thuringiensis are identified, and a new microbial resource is provided for biological prevention and treatment of the plutella xylostella. The preservation number of the provided bacillus thuringiensis B-T-3 strain is CCTCC (China Center For Type Culture Collection) No: M20252325. The high-toxicity bacillus thuringiensis obtained by screening has remarkable and excellent insecticidal activity on 2-instar and 3-instar larvae of plutella xylostella. Under the same treatment concentration, the corrected death rate is obviously higher than that of other isolated strains.

The application discloses a preparation method of an extractive for preventing and treating citrus anthracnose, and comprises the following steps: drying garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed, and then crushing the dried garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed; adding the crushed garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed into boiling water and stirring until uniform; continuing to cook; freezing; thawing after the freezing is completed; obtaining a freeze-thawed material; adding sodium dihydrogen phosphate into the freeze-thawed material; placing the freeze-thawed material in a fermentation tank; inoculating bacillus subtilis and bacillus mycoides for aerobic fermentation; inoculating bacillus licheniformis, bacillus laterosporus and bacillus thuringiensis for aerobic fermentation; completing the aerobic fermentation; carrying out anaerobic fermentation on the fermented material; stirring the fermented material and pure water until uniform; filtering after standing; taking filter residue and precipitate as extractive A; adjusting the PH value of filtrate to obtain extractive B; adding bagasse powder into the extractive A, compacting, packaging and bagging; and sealing and storing the extractive B. The extractive prepared by the application can efficiently prevent and treat citrus anthracnose, and has the characteristics of low production cost and no pollution to citrus planting environment.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera and Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The application discloses a fermentation culture method of Bacillus thuringiensis Bt64 strain and belongs to the technical field of microbial culture. The orthogonal test is adopted to optimize the culture medium components of the Bacillus thuringiensis Bt64 strain, high-quality and low-cost culture medium with corn powder, corn starch and potato starch as carbon sources and soybean powder and fish meal as nitrogen sources is determined, and the optimal component ratio is determined. Compared with the LB culture medium, the Bt64 strain cultured by using the culture medium is amplified fast and declines slowly. Based on the culture medium, the temperature, inoculation ratio, time, ventilation amount and rotating speed of the fermentation culture of the Bt64 strain are further optimized, and the optimal culture conditions suitable for the rapid and large-scale proliferation of the Bt64 strain are determined. The application provides a reference basis and technical support for the fermentation tank culture, industrial development and large-scale production of the Bacillus thuringiensis Bt64 strain.

The disclosure provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidopteran and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

This invention discloses a carbon-based biological liquid organic water-soluble fertilizer, its preparation method, and its application, belonging to the field of bio-organic fertilizer technology. The water-soluble fertilizer of this invention comprises the following raw materials in parts by weight: 15-25 parts seaweed extract, 10-15 parts potassium humate, 5-10 parts compound microbial inoculant, 3-5 parts potassium dihydrogen phosphate, 2-4 parts potassium nitrate, 2-3 parts urea, 2-3 parts calcium nitrate, 1-2 parts magnesium sulfate heptahydrate, 1-1.5 parts trace element substances, 1-2 parts sodium dodecylbenzenesulfonate, 0.5-1 part potassium sorbate, 0.5-1 part organosilicon surfactant, and water to a total of 100 parts. This invention screens three strains of Pseudomonas sevivar, Bacillus microepiploicus, and Bacillus thuringiensis, which function in three dimensions: promoting growth, improving the rhizosphere environment, and preventing and controlling pests and diseases. These strains are combined with organic components such as seaweed extract and potassium humate in fertilizers, as well as other components, to form a comprehensive system of "nutrition + growth promotion + disease resistance + quality improvement", which significantly improves the yield and quality of grapes.

The disclosure provides nucleic acids, and variants and fragments thereof, derived from strains of Bacillus thuringiensis encoding variant polypeptides having increased pesticidal activity against insect pests, including Lepidoptera and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention relates to bacillus thuringiensis KHWY-1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.33400. The strain is used for fermenting feathers for 20 hours under the conditions that the temperature is 25 DEG C and the pH value is 8, the soluble protein content reaches 691.37 mu g / mL, and the feather degradation rate reaches 90% or above. The invention also relates to a new application of the bacillus thuringiensis KHWY-1 in degrading the feather fermentation filtrate of the white feather chicken as a cosmetic raw material, and the additional value of feather degradation products is improved.

A pest control composition includes humic acid, wherein the humic acid has a concentration of phenolic groups of 0.19 to 1.30 millimoles per gram of the humic acid, bacillus thuringiensis and water.

This invention discloses a strain of Bacillus thuringiensis H68 and its applications. This invention relates to the field of microbiology, providing a Bacillus thuringiensis or its progeny, wherein the Bacillus thuringiensis is Bacillus thuringiensis (… Bacillus thuringiensis The strain number is H68, and its accession number at the China Center for Type Culture Collection is CCTCC NO: M 20251171. The Bacillus thuringiensis H68 provided by this invention exhibits broad-spectrum antibacterial effects against clinically pathogenic bacteria, making it of significant application value in drug development. It can provide new drug options for clinically pathogenic bacterial infections, and plays a crucial role in reducing the risk of drug-resistant bacterial transmission and decreasing drug dependence.