25 results about "Aureobasidium sp." patented technology

The invention provides a bacillus thuringiensis strain, a fermentation culture method and application of the bacillus thuringiensis strain. The strain is preserved in the China Center for Type Culture Collection on September 28, 2011, the preservation address is No. 299 on eight road, Wuchang District, Wuhan City, Hubei Province, the preservation number is CCTCC No: M 2011334, and the preservation date is September 28, 2011. The strain cultured by the fermentation method has efficient, specific and stable insecticidal activity on culex, aedes and other mosquitoes, has the advantages of low preparation cost, environmental friendliness and the like, can be developed into a biological insecticide for preventing and killing mosquitoes and preventing and controlling malaria, dengue fever, chikungunya and other mosquito-borne infectious diseases, and meets the requirements of green and safe prevention and control in China.

The invention discloses a bacillus thuringiensis CMS-BT17, which is classified and named as bacillus thuringiensis, is preserved in the China General Microbiological Culture Collection Center (CGMCC), has the preservation number of CGMCC 35313, and is preserved on July 21, 2025. The bacillus thuringiensis strain CMS-BT17 directly separated and screened from a zizania aquatica soil sample in Haikou City of Hainan carries four insecticidal protein coding genes of Cry8Ca, Cry8Da, Spp1Aa and Vpb4Ca, not only is high in insecticidal toxicity and good in insecticidal effect, but also has a certain effect of inhibiting tomato fungal diseases, can be widely applied to prevention and control of agricultural diseases and pests, and has a wide application prospect. The sustainable development of green agricultural production is promoted.

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

The present invention describes capsule compositions consisting of crosslinked chitosan, beta-glucan or alginate, and encapsulates an environmentally compatible ingestible larvicide, especially larvicidal agents such as essential oils or other ingestible larvicides such as Bacillus thuringiensis which are lethal to the larvae of pest insects, especially mosquito larvae.

This application relates to the field of microbial strain technology, specifically disclosing a Bacillus thuringiensis HP-SYJ2 strain and its applications. The Bacillus thuringiensis HP-SYJ2 strain is deposited at the China Center for Type Culture Collection (CCTCC), accession number: CCTCC NO: M2025343, deposit date: March 3, 2025, address: Wuhan University, Wuhan, China. The strain of this application can be used to prepare insecticides for controlling underground pests, which is of great significance for the biological control of plant diseases and pests.

The invention discloses a carbon-based biological liquid organic water-soluble fertilizer as well as a preparation method and application thereof, and belongs to the technical field of biological organic fertilizers. The water-soluble fertilizer comprises the following raw materials in parts by weight: 15-25 parts of a seaweed extract, 10-15 parts of potassium fulvate, 5-10 parts of a compound microbial agent, 3-5 parts of potassium dihydrogen phosphate, 2-4 parts of potassium nitrate, 2-3 parts of urea, 2-3 parts of calcium nitrate, 1-2 parts of magnesium sulfate heptahydrate, 1-1.5 parts of a trace element-containing substance, 1-2 parts of sodium dodecyl benzene sulfonate and 0.5-1 part of potassium sorbate. The total amount of the components is 100 parts. According to the invention, three strains such as pseudomonas sevivaldi, bacillus pumila and bacillus thuringiensis are screened to play roles in three dimensions of promoting growth, improving rhizosphere environment and preventing and treating diseases and insect pests respectively, and are combined with organic components such as seaweed extract and potassium fulvate in the fertilizer and other components, so that the fertilizer has a good application prospect. A comprehensive system of nutrition, growth promotion, disease resistance and quality improvement is formed, and the yield and quality of grapes are remarkably improved.

The invention discloses a microbial preparation for preventing and treating plant root-knot nematode as well as a preparation method and application thereof, and belongs to the technical field of microbial preparations. The microbial preparation for preventing and treating the plant root-knot nematode is of a double-layer coating structure and comprises an inner layer and an outer layer, the inner layer comprises phaffia rhodozyma fermentation liquor, paecilomyces lilacinus fermentation liquor, bacillus subtilis fermentation liquor, bacillus thuringiensis fermentation liquor, beauveria bassiana fermentation liquor and an inner layer carrier material; the outer layer comprises a streptomyces densely-rotating fermentation liquid and an outer layer carrier material; according to the application, the phaffia rhodozyma, the paecilomyces lilacinus, the bacillus subtilis, the bacillus thuringiensis fermentation liquor, the beauveria bassiana fermentation liquor and the streptomyces dense are compounded, and the strains have a synergistic effect, so that functional complementation is realized; a double-layer coating structure is adapted to acid soil, guarantees the initial activity of the strain and improves the survival efficiency of the strain on one hand, and can prolong the shelf life and the function exerting period on the other hand, so that long-acting pest control is realized.

This invention discloses a method for the domestication, isolation, purification, and application of a low-temperature resistant straw-degrading bacterium, belonging to the fields of agricultural microbiology technology and waste resource utilization. The domestication method specifically includes sample preparation, straw pretreatment, culture medium preparation, and multiple rounds of low-temperature domestication culture. During this process, the straw weight loss rate is used as an indicator to detect the degradation efficiency of the bacterial community after each round of domestication. The obtained low-temperature resistant straw-degrading bacterium was deposited on April 27, 2026, at the China Center for Type Culture Collection (CCTCC), located at No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province; the accession number is CCTCC NO: M2026814; the taxonomic name of the low-temperature resistant straw-degrading bacterium is *Brucea buddinga* X4-8. (Aureobasidium sp. X4-8) The domestication method of this invention actively enhances the low-temperature adaptability and degradation activity of microbial communities by combining exogenous amino acid induction with systematic cyclic domestication. Furthermore, this invention also discloses a method for isolating and purifying low-temperature resistant straw-degrading bacteria and its application.

The invention discloses bacillus thuringiensis CMS-5E8, which is classified and named as bacillus thuringiensis, and is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, the preservation number is CGMCC 35311, and the preservation date is July 21, 2025. The strain carries 16 insecticidal protein coding genes, namely Cry1Aa, Cry1Ah, Cry1Be, Cry1If, Cry1Na, Cry1Ea, Cry1La, Cry2Ab, Cry2Ah, Vip3Aa, Vip3Ad, Spp1Aa, Vpb4Ca, Tpp78Ba, Mpp46Ab and Mpp3Aa, not only is high in insecticidal toxicity and good in insecticidal effect, but also has a certain effect of inhibiting fungal diseases, and can be widely applied to prevention and treatment of agricultural diseases and insect pests.

The application discloses a saline-alkali soil special rice bio-organic fertilizer, which comprises halomonas, bacillus subtilis, paenibacillus polymyxa, bacillus thuringiensis, lactobacillus plantarum, longovis rubescens and organic fertilizer. In actual preparation, the microorganism is expanded to obtain a microbial agent, then the rotten cow dung and auxiliary materials are uniformly mixed, granulation drying is carried out, and after cooling treatment, the microbial agent and a coating agent mixed solution are added to carry out coating treatment, and then packaging is carried out. After the finished product is packaged, it can be used after being stored in a warehouse for a period of time. The microbial fertilizer has high organic matter content and active bacteria quantity, and in combination with conventional irrigation and fertilization measures, can improve the soil structure of saline-alkali soil, reduce the soil salt content and pH, enhance the stress resistance of crops, promote the growth of rice, and increase the yield, so that the effects of improving soil, fertilizing, resisting stress and promoting growth are achieved.

The invention discloses a compound microbial agent rich in beta-glucan as well as a preparation method and application of the compound microbial agent, and belongs to the technical field of agricultural biology. According to the microbial agent, an aureobasidium pullulans-rhizobium pullulans-bacillus subtilis-bacillus thuringiensis quaternary synergistic system is constructed, specific auxiliary materials, carriers and a coating technology are supplemented, and under the drought stress condition, the microbial agent can remarkably promote the growth (plant height and biomass) of the pakchoi, so that the yield of the pakchoi is increased, and the yield of the pakchoi is increased. The yield of the pakchoi is increased, the quality is improved (the content of soluble sugar and protein is increased), the chlorophyll content and the photosynthetic efficiency are improved, the antioxidant enzyme activity is enhanced, cell membrane damage is reduced, the physical and chemical properties of soil are improved, and a new effective solution is provided for agricultural green drought resistance.

According to the bacillus thuringiensis and the application thereof in preventing and treating the plutella xylostella, the bacillus thuringiensis with specific activity on the plutella xylostella is screened, the types of insecticidal proteins of the bacillus thuringiensis are identified, and a new microbial resource is provided for biological prevention and treatment of the plutella xylostella. The preservation number of the provided bacillus thuringiensis B-T-3 strain is CCTCC (China Center For Type Culture Collection) No: M20252325. The high-toxicity bacillus thuringiensis obtained by screening has remarkable and excellent insecticidal activity on 2-instar and 3-instar larvae of plutella xylostella. Under the same treatment concentration, the corrected death rate is obviously higher than that of other isolated strains.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera and Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The disclosure provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidopteran and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

This invention discloses a carbon-based biological liquid organic water-soluble fertilizer, its preparation method, and its application, belonging to the field of bio-organic fertilizer technology. The water-soluble fertilizer of this invention comprises the following raw materials in parts by weight: 15-25 parts seaweed extract, 10-15 parts potassium humate, 5-10 parts compound microbial inoculant, 3-5 parts potassium dihydrogen phosphate, 2-4 parts potassium nitrate, 2-3 parts urea, 2-3 parts calcium nitrate, 1-2 parts magnesium sulfate heptahydrate, 1-1.5 parts trace element substances, 1-2 parts sodium dodecylbenzenesulfonate, 0.5-1 part potassium sorbate, 0.5-1 part organosilicon surfactant, and water to a total of 100 parts. This invention screens three strains of Pseudomonas sevivar, Bacillus microepiploicus, and Bacillus thuringiensis, which function in three dimensions: promoting growth, improving the rhizosphere environment, and preventing and controlling pests and diseases. These strains are combined with organic components such as seaweed extract and potassium humate in fertilizers, as well as other components, to form a comprehensive system of "nutrition + growth promotion + disease resistance + quality improvement", which significantly improves the yield and quality of grapes.

The disclosure provides nucleic acids, and variants and fragments thereof, derived from strains of Bacillus thuringiensis encoding variant polypeptides having increased pesticidal activity against insect pests, including Lepidoptera and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention relates to bacillus thuringiensis KHWY-1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.33400. The strain is used for fermenting feathers for 20 hours under the conditions that the temperature is 25 DEG C and the pH value is 8, the soluble protein content reaches 691.37 mu g / mL, and the feather degradation rate reaches 90% or above. The invention also relates to a new application of the bacillus thuringiensis KHWY-1 in degrading the feather fermentation filtrate of the white feather chicken as a cosmetic raw material, and the additional value of feather degradation products is improved.

This invention discloses a strain of Bacillus thuringiensis H68 and its applications. This invention relates to the field of microbiology, providing a Bacillus thuringiensis or its progeny, wherein the Bacillus thuringiensis is Bacillus thuringiensis (… Bacillus thuringiensis The strain number is H68, and its accession number at the China Center for Type Culture Collection is CCTCC NO: M 20251171. The Bacillus thuringiensis H68 provided by this invention exhibits broad-spectrum antibacterial effects against clinically pathogenic bacteria, making it of significant application value in drug development. It can provide new drug options for clinically pathogenic bacterial infections, and plays a crucial role in reducing the risk of drug-resistant bacterial transmission and decreasing drug dependence.

The application discloses an application of Bacillus thuringiensis in preparing a biocontrol and trapping agent for preventing and treating galleria mellonella. The Bacillus thuringiensis is named Bacillus thuringiensis BiotG03, and has a preservation number of CGMCC No. 26127 in the China General Microbiological Culture Collection Center. The application further discloses a biocontrol and trapping agent for preventing and treating galleria mellonella, which comprises the Bacillus thuringiensis BiotG03 and an attractant. The application of the Bacillus thuringiensis in preventing and treating galleria mellonella has a very important practical significance in guaranteeing the quality of bee products, reducing economic losses in beekeeping production and protecting Apis cerana.

The invention discloses bacillus thuringiensis CMS-B11, the classification name of the bacillus thuringiensis CMS-B11 is bacillus thuringiensis, the bacillus thuringiensis CMS-B11 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC 35312, and the preservation date is July 21, 2025. The bacillus thuringiensis strain CMS-B11 directly separated and screened from a coastal soil sample in Hainan carries three insecticidal protein coding genes, namely Cry3Aa, Ssp1Aa and Vpb4Ca, not only is high in insecticidal toxicity and good in insecticidal effect, but also has a certain effect of inhibiting fungal diseases, can be widely applied to prevention and control of agricultural diseases and pests, and has a good application prospect. The sustainable development of green agricultural production is promoted.

This invention discloses a strain of Bacillus thuringiensis and its application in the degradation of lignocellulose, belonging to the field of microbial technology. The microbial classification name is Bacillus thuringiensis, the strain name is LMU-81, and the accession number is CGMCC NO.36215. Strain LMU-81 was isolated from leaf litter soil and accumulated decaying wood in mangrove wetlands. It exhibits high lignocellulose enzyme activity, particularly lignin peroxidase (LiP), and can be used as a microbial inoculant in combination with other microorganisms to form a compound inoculant for the degradation of lignocellulose in sugarcane bagasse and other materials.

The invention discloses bacillus with a growth promoting function on plant salt and alkali and heavy metal stress and application, and belongs to the technical field of microorganisms. The strain is preserved in China Center for Type Culture Collection, the preservation address is Wuhan University, Wuchang District, Wuhan City, Hubei Province, the preservation date is January 14, 2026, the preservation number is CCTCC NO: M2026103, and the strain is classified and named as Bacillus thuringiensis BL01. The strain provided by the invention has a probiotic effect of promoting germination and growth of plant seeds under stress of salt, alkali and heavy metals, can be used for phytoremediation of soil with high salt, alkali and heavy metal concentration, and has the advantages of low cost and high efficiency; the fertilizer can be used as a bio-fertilizer, achieves the purpose of improving the yield and quality, and has the advantages of low cost, no pollution to the environment, safety to human and livestock and the like.

The present invention provides a composite biocontrol bacterial seed treatment agent containing Streptococcus thermophilus, a preparation method and an application, belonging to the technical field of biological pesticides. The seed treatment agent is a solid powder consisting of 80% to 90% effective active component and 10% to 20% auxiliary materials. The effective active component is an equal-proportion composite bacterial fermentation broth concentrate solidification product of Bacillus subtilis, Bacillus cereus, Bacillus thuringiensis and Streptococcus thermophilus. In the present invention, the fermentation concentrate of multiple bacterial strains is mixed with a carrier and spray- dried to obtain the composite bacterial fermentation broth concentrate solidification product; then the solidification product is mixed with the auxiliary materials to obtain the present invention.

The application belongs to the technical field of heavy metal pollution treatment, and relates to a preparation of a weak alkaline soil cadmium passivator and a method for passivating soil lead. The weak alkaline soil cadmium passivator mainly comprises the following components in parts by weight: graphene oxide modified biochar 20-30 parts, montmorillonite 5-10 parts, bentonite 5-10 parts and microbial inoculum 5-10 parts, wherein the microbial inoculum is a composite inoculum composed of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus thuringiensis and Pseudomonas nitroreducens. The weak alkaline soil cadmium passivator has a good repairing effect on cadmium and lead in contaminated soil, can significantly improve the physicochemical properties of cadmium and / or lead contaminated soil, increase the soil organic matter content, reduce the migration in the environment, reduce the cadmium content in crops, and has great market promotion value.

An isolated polynucleotide which encodes a protein comprising a polypeptide sequence exhibiting at least 88% sequence identity to the amino acid sequence as set forth in SEQ ID NO.6 over a length of at least 300 amino acids and that can be used to inhibit Hemipteran insects.