Acne-removing external preparation and preparation method thereof

Abstract

The invention provides an acne-removing external preparation and a preparation method thereof. The acne-removing external preparation comprises the following raw materials in parts by weight: 5-15 parts of an acne-removing composition and 50-95 parts of auxiliary materials, the acne-removing composition is obtained by sequentially carrying out enzymolysis and fermentation on traditional Chinese medicine raw materials, the traditional Chinese medicine raw materials comprise paris polyphylla, curcuma zedoary, rhizoma sparganii, rhizoma bletillae and selfheal in a mass ratio of (0.5-0.8): (1-3): (3-5): (6-8): A composite enzymolysis agent is used for enzymolysis, and the composite enzymolysis agent comprises inulase, beta-amylase, streptavidin and hemicellulase according to the mass ratio of 1: (1.5-3): (5-6.5): (8-10); a composite leavening agent is used for fermentation, and the composite leavening agent comprises citrobacter, bifidobacterium longum, bacillus thuringiensis and bacillus xylinum according to the mass ratio of (0.1-0.3): (1-3): (0.5-0.8): 1. The acne-removing external preparation prepared by the invention has good safety and acne-removing effect.

Description

Technical Field

[0001] The invention belongs to the field of cosmetics, and particularly relates to an anti-acne external preparation and a preparation method thereof. Background Art

[0002] Acne, also known as acne, is a common skin disease, which is mainly manifested by chronic inflammation of the hair follicle sebaceous gland system. Acne usually appears on the face, neck, chest, back and shoulders, which have more sebaceous glands. Common topical medicines for acne removal include retinoic acid, benzoyl peroxide, and antibiotics. Among them, retinoic acid drugs can help reduce the accumulation of keratin and promote the shedding of the stratum corneum, but may increase the skin's sensitivity to light and cause side effects such as skin redness; benzoyl peroxide has a bactericidal effect and can reduce the number of Propionibacterium acnes, but it may also cause dry skin, peeling and other discomforts; antibiotics can reduce inflammation, but long-term use may lead to bacterial resistance.

[0003] Since the above-mentioned acne-removing topical pharmaceutical products have the disadvantages of strong irritation and strong drug resistance, more and more people tend to use skin care products containing natural ingredients to remove acne. These products are relatively mild and less irritating to the skin. According to their main active ingredients, natural acne-removing topical preparations can be divided into plant essential oil products, Chinese herbal extract products, natural water-soluble products and natural polyphenol products. Among them, plant essential oil products include tea tree oil, lavender oil, peppermint oil, etc.; Chinese herbal extract products include Centella asiatica, Phellodendron chinense, honeysuckle, etc.; natural water-soluble products include niacinamide (vitamin B3), betaine, etc.; natural polyphenol products include grape seed extract, which is rich in antioxidants and can help resist free radical damage and protect the skin from environmental damage.

[0004] Although acne-removing topical products with natural ingredients have many advantages, such as acne removal, moisturizing, and good skin affinity, they also have some disadvantages in actual use, such as the acne removal effect is not significant or the safety is not very high. Summary of the invention

[0005] The purpose of the present invention is to provide an acne-removing external preparation and a preparation method thereof, so as to solve the problems of the safety and unsatisfactory acne-removing effect of the above-mentioned acne-removing external products with natural ingredients.

[0006] In order to achieve the above-mentioned object, the first invention of the present invention provides an acne-removing external preparation, which comprises the following raw materials in parts by weight: 5 to 15 parts of the acne-removing composition and 50 to 95 parts of auxiliary materials;

[0007] The anti-acne composition is obtained by sequentially performing enzymatic hydrolysis and fermentation on Chinese medicinal raw materials;

[0008] The Chinese medicinal raw materials include Paris polyphylla, Curcuma zedoaria, Trigonella ternata, Bletilla striata and Prunella vulgaris in a mass ratio of (0.5-0.8):(1-3):(3-5):(6-8):10;

[0009] The enzymatic hydrolysis uses a composite enzymatic hydrolyzer, which includes inulinase, β-amylase, pronase and hemicellulase in a mass ratio of 1: (1.5-3): (5-6.5): (8-10);

[0010] The fermentation uses a composite fermentation agent, which includes Citrobacter acidophilus, Bifidobacterium longum, Bacillus thuringiensis and Bacillus waxyii in a mass ratio of (0.1-0.3):(1-3):(0.5-0.8):1.

[0011] Natural acne-removing products generally refer to products that use plant extracts or other natural ingredients to help treat acne or pimples. The advantage of these products is that they often contain fewer chemical additives and may be gentler and non-irritating to the skin. However, compared with chemically synthesized drugs, the mechanism of action of natural ingredients may be weaker, resulting in slower acne removal. In view of the above problems, the present invention proposes a method of forming a formula with multiple Chinese medicine components, and the synergistic effect between multiple Chinese medicine raw materials improves the acne removal effect. The Chinese medicinal raw materials in the present invention include Paris polyphylla, Curcuma zedoaria, Rhizoma trilobata, Bletilla striata and Herba Prunellae, wherein Paris polyphylla has the effects of clearing away heat and detoxicating, reducing swelling and relieving pain, it can help alleviate the redness and pain caused by acne, and help remove bacteria and toxins on the skin surface; Curcuma zedoaria has the effect of promoting blood circulation and removing blood stasis, it can promote blood circulation, help improve the metabolism of local skin tissue, and accelerate the recovery of the inflammatory site; Rhizoma trilobata is usually used for breaking blood and promoting qi, it can help improve the qi and blood circulation of the skin, promote the blood circulation of the acne site, and accelerate the maturation and discharge of acne; Bletilla striata has the effects of astringing and stopping bleeding, promoting tissue regeneration and healing sores, it can promote the repair of damaged skin, reduce the formation of scars, and help the skin return to a normal state; Herba Prunellae has the effects of clearing the liver and improving eyesight, dispersing nodules and reducing swelling, it can help regulate the internal environment, and reduce the acne problem caused by excessive sebum secretion. The combined application of Paris polyphylla and Herba Prunellae can strengthen the anti-inflammatory and antibacterial effects, reduce bacterial infections on the skin, and prevent acne from worsening. The combination of Curcuma and Tripterygium wilfordii can improve the microcirculation of the skin, promote blood flow in the acne area, help eliminate toxins, and accelerate the disappearance of inflammation. The repair function of Bletilla striata can be combined with the anti-inflammatory and blood circulation effects of other medicinal materials to accelerate the healing of damaged skin tissue and reduce scar formation. Therefore, the present invention uses five kinds of Chinese medicinal materials for compatibility, which can improve the acne removal effect.

[0012] The present invention further studies and finds that although the use of a variety of Chinese medicinal raw materials can improve the acne removal effect, the addition of extracts of Chinese medicinal raw materials to acne removal products cannot significantly improve the acne removal effect, and there are certain defects of insufficient safety, which seriously affects the actual use of acne removal external preparations. In view of this problem, the present invention successively performs enzymatic hydrolysis treatment of a composite enzymatic agent and fermentation treatment of a composite fermentation agent on the Chinese medicinal raw materials, thereby improving the acne removal and safety of the acne removal external preparation.

[0013] The present invention first uses four enzymolysis agents, inulinase, β-amylase, pronase and hemicellulase, to enzymolyze the Chinese medicine raw materials at the same time. During the enzymolysis process, each enzyme has its specific substrate range and mechanism of action, wherein inulinase can decompose inulin-like substances; β-amylase mainly acts on starch; pronase can decompose protein; and hemicellulase can decompose the hemicellulose components in the plant cell wall. The use of multiple enzymolysis agents can more comprehensively decompose various macromolecular substances in Chinese medicine, converting them into smaller, more easily absorbed small molecules, thereby improving the activity of the acne-removing composition. The simultaneous action of multiple enzymolysis agents in the present invention can increase the release rate of the effective ingredients of Chinese medicine, so that more active substances can be extracted, which not only improves the effectiveness of the acne-removing composition, but also means that more active ingredients can remain stable in different environments.

[0014] The present invention continues to ferment the enzymatic hydrolyzate, and the composite fermentation agent includes Citrobacter, Bifidobacterium longum, Bacillus thuringiensis and Bacillus waxyii. During the fermentation process, various fermentation agents can produce different metabolites, which may have various biological activities such as antioxidant, anti-inflammatory, and antibacterial, which help to improve the effectiveness of the acne-removing composition. At the same time, the presence of various metabolites may also form a complex interaction network to enhance the overall effect of the composition. The fermentation process may change the physical properties of the enzymatic hydrolyzate, such as viscosity, fluidity, etc. These changes can make the acne-removing composition more suitable for the preparation of skin care products, thereby improving its stability and use effect under different environments.

[0015] Preferably, the enzymatic hydrolysis step of the Chinese medicine raw material is:

[0016] S11: Weigh Paris polyphylla, Curcuma zedoaria, Tripterygium wilfordii, Bletilla striata and Prunella vulgaris in order according to the mass ratio, dry at 60-80° C. for 1-5 h, and grind into Chinese medicine powder;

[0017] S12: adding the Chinese medicine powder into water, then adding the composite enzymatic hydrolyzing agent, and performing enzymatic hydrolysis in a water bath at 35-40° C. for 0.5-3 h to obtain a first enzymatic hydrolyzing solution;

[0018] S13: Turn on the computer microwave-ultrasonic combined catalytic synthesizer to perform ultrasonic-microwave assisted enzymolysis on the first enzymolysis solution, and then perform enzyme inactivation treatment after continuing the enzymolysis to obtain a second enzymolysis solution;

[0019] S14: centrifuging the second enzymatic hydrolysate, taking the supernatant, and freeze-drying it to obtain an enzymatic hydrolysate.

[0020] The present invention has also been further studied and found that once the acne-removing topical preparation is opened and used, it will inevitably come into contact with oxygen in the air. However, once the acne-removing topical preparation is oxidized, it is likely to affect its use effect. In view of this problem, the present invention further performs ultrasound-microwave assisted enzymolysis on the first enzymolysis solution in addition to the original direct enzymolysis process of the composite enzymolysis agent during the enzymolysis process. In the ultrasound-microwave assisted enzymolysis process, ultrasound and microwaves can increase the solubility of the enzymolysis product, making it easier to disperse in the solution, avoiding precipitation or aggregation, and helping to improve the uniformity and stability of the acne-removing composition. The auxiliary effects of ultrasound and microwaves can also help further release the active ingredients in the Chinese medicine raw materials.

[0021] Preferably, in step S12, the mass ratio of the Chinese medicine powder to water is 1:(10-30), and the mass ratio of the Chinese medicine powder to the composite enzymatic hydrolyzing agent is 1:(0.1-0.5).

[0022] Preferably, in step S13, the insertion depth of the ultrasonic probe is fixed, the microwave power is 300-800 W, the ultrasonic power is 80-120 W, the ultrasonic-microwave treatment temperature is 35-40° C., and the treatment time is 5-8 min.

[0023] Preferably, the fermentation step of the enzymatic hydrolysate is:

[0024] S21: adding a composite fermentation agent to the enzymatic hydrolyzate, then adding water, sealing, and fermenting at 28-35° C. for 3-8 days to obtain a fermentation liquid;

[0025] S22: filtering the fermentation liquid to obtain a filtrate, sterilizing the filtrate to inactivate the bacteria;

[0026] S23: centrifuging the sterilized filtrate, collecting the supernatant, and freeze-drying it to obtain an acne-removing composition.

[0027] Preferably, in step S21, the inoculation amount of the composite leavening agent is 5 to 10 wt %, and the amount of water added is 10 to 15 times the amount of the enzymatic hydrolysis product.

[0028] Preferably, in step S22, sterilization is performed at a temperature of 100 to 120°C for 20 to 60 minutes.

[0029] Preferably, the auxiliary material is at least one of a solvent, an emulsifier, a co-emulsifier, an antioxidant, a thickener, a preservative, a moisturizer, an emollient, a flavor, a chelating agent, and a pH adjuster.

[0030] Preferably, the solvent is at least one of water, propylene glycol, methylpropylene glycol, dipropylene glycol, pentylene glycol, and isoprene glycol;

[0031] The emulsifier is at least one of glycosides, sucrose esters, amino acids, glycerides, polyglycerol esters, and polyethylene glycols;

[0032] The co-emulsifier is cetearyl alcohol;

[0033] The antioxidant is at least one of tocopherol, tocopherol acetate, pentaerythritol tetraester, butylated hydroxytoluene, and sodium metabisulfite;

[0034] The thickener is one of xanthan gum, carrageenan, carbomer, high molecular weight cellulose, sodium polyacrylate, acrylic acid (ester) copolymer, and hydroxypropyl starch phosphate;

[0035] The preservative is one of phenoxyethanol, parabens, benzoic acid and its salts, dehydroacetic acid and its salts, and sodium diacetate;

[0036] The moisturizing agent is at least one of glycerin, polyglyceryl ether-26, butylene glycol, polyglyceryl-6, and 1,3-propylene glycol;

[0037] The emollient is at least one of vegetable oil, synthetic oil, animal oil, and mineral oil;

[0038] The chelating agent is at least one of EDTA-2Na, EDTA-3Na, and octanoylhydroxamic acid;

[0039] The pH adjuster is at least one of triethanolamine, aminomethyl propanol, arginine, and inorganic bases.

[0040] Preferably, the emulsifier is at least one of steareth-2, steareth-21, glyceryl stearate, glyceryl stearate / PEG-100 stearate, polysorbate 60, cetearyl glucoside, 14-22 alcohol, C12-20 alkyl glucoside, cetyl stearyl alcohol, coconut glucoside, and ceteareth-20.

[0041] It should be noted that the auxiliary materials in the present invention include but are not limited to the above-mentioned ones, and also include oils and absorption promoters, etc. The oil is at least one of isopropyl myristate, hydrogenated polyisobutylene, and polydimethylsiloxane, and the absorption promoter is laurocapram. The acne-removing composition can be added to the preparation process of any acne-removing external preparation according to different usage methods.

[0042] The second aspect of the present invention provides a method for preparing an acne-removing external preparation, wherein the acne-removing composition and auxiliary materials are mixed and stirred evenly according to parts by weight to obtain the acne-removing external preparation, which is one of an aqueous preparation form, a gel preparation form or an emulsion preparation form.

[0043] The water dosage forms include: lotion, repair water, moisturizing water, toner or mask, etc.; the gel dosage forms include skin care essence, skin care gel, gel-like sleeping mask, etc.; the emulsion dosage forms include lotion, cream, etc. In the present invention, the type of auxiliary materials can be selected according to the prepared dosage form, and the corresponding acne-removing external preparation can be synthesized according to general technical means.

[0044] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0045] 1. The acne-removing external preparation of the present invention adopts a variety of Chinese medicinal raw materials consisting of Paris polyphylla, Curcuma zedoaria, Trillium, Bletilla striata and Prunella vulgaris. These medicinal materials have the effects of anti-inflammatory, antibacterial, and promoting wound healing. Through specific proportions, the advantages of each component can be comprehensively utilized to achieve better therapeutic effects.

[0046] 2. The Chinese medicine raw materials in the present invention are processed by enzymolysis and fermentation in sequence, which can improve the release rate of the effective ingredients of the medicinal materials. The composite enzymolysis agent used in the enzymolysis process can effectively decompose the macromolecular substances in the medicinal materials, making them easier to be absorbed by the skin. The composite fermentation agent used in the fermentation process helps to produce new bioactive substances, further improving the efficacy of the preparation.

[0047] 3. The present invention also uses ultrasound-microwave assisted treatment during the enzymatic hydrolysis process, which not only helps to accelerate the reaction speed, but also ensures the uniformity of the enzymatic hydrolyzate, thereby improving the stability and consistency of the final product.

[0048] 4. The Chinese medicinal raw materials of the present invention form complex organic matter and microbial metabolites during the enzymatic hydrolysis and fermentation process, and a balance is formed between the two. This equilibrium state helps to maintain the stability of the product under different environments and reduce changes in product performance due to changes in external conditions. DETAILED DESCRIPTION

[0049] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0050] Example 1

[0051] This embodiment provides an acne-removing external preparation, which includes an acne-removing composition and auxiliary materials.

[0052] The preparation method of the anti-acne composition comprises the following steps:

[0053] (1) The enzymatic hydrolysis steps of Chinese medicine raw materials are:

[0054] S11: Weigh Paris polyphylla, Curcuma zedoaria, Tripterygium wilfordii, Bletilla striata and Prunella vulgaris in order according to a mass ratio of 0.6:2:4:7:10, dry at 65°C for 3h, and grind into Chinese medicine powder;

[0055] S12: adding the Chinese medicine powder to water, then adding the composite enzymolysis agent, and performing enzymolysis in a water bath at 38° C. for 1.5 h to obtain a first enzymolysis solution; the mass ratio of the Chinese medicine powder to water is 1:20, and the mass ratio of the Chinese medicine powder to the composite enzymolysis agent is 1:0.3;

[0056] The composite enzymatic hydrolyzer is inulinase, β-amylase, pronase and hemicellulase in a mass ratio of 1:2:6:9;

[0057] Inulinase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10241;

[0058] β-Amylase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10006;

[0059] Pronase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10014;

[0060] Hemicellulase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10045;

[0061] S13: Turn on the computer microwave-ultrasonic combined catalytic synthesizer to perform ultrasonic-microwave assisted enzymolysis on the first enzymolysis solution, wherein the insertion depth of the ultrasonic probe is fixed, the microwave power is 500 W, the ultrasonic power is 100 W, the ultrasonic-microwave treatment temperature is 38° C., the treatment time is 6 min, and the enzymolysis is continued and then the enzyme is inactivated to obtain a second enzymolysis solution;

[0062] S14: centrifuging the second enzymatic hydrolysate, taking the supernatant, and freeze-drying it to obtain an enzymatic hydrolysate.

[0063] (2) The fermentation steps of the enzymatic hydrolysis product are:

[0064] S21: adding a composite fermentation agent to the enzymatic hydrolysate at an inoculation rate of 8 wt %, then adding 12 times the amount of water as the enzymatic hydrolysate, sealing, and fermenting at 30° C. for 5 days to obtain a fermentation liquid;

[0065] The composite fermentation agent is Citrobacter, Bifidobacterium longum, Bacillus thuringiensis and Bacillus xylella fastidiosa in a mass ratio of 0.2:2:0.7:1;

[0066] Citrobacter rodentium was purchased from Henan Industrial Microbial Strain Engineering Technology Research Center, model number BNCC369613;

[0067] Bifidobacterium longum was purchased from Henan Industrial Microbial Strain Engineering Technology Research Center, model number BNCC186483;

[0068] Bacillus thuringiensis was purchased from Henan Industrial Microbial Strain Engineering Technology Research Center, model number BNCC336646;

[0069] Bacillus xylella was purchased from Henan Industrial Microbial Strain Engineering Technology Research Center, model BNCC280126;

[0070] S22: filtering the fermentation broth to obtain a filtrate, and sterilizing the filtrate at 110° C. for 45 minutes to inactivate the bacteria;

[0071] S23: centrifuging the sterilized filtrate, collecting the supernatant, and freeze-drying it to obtain an acne-removing composition.

[0072] A method for preparing an acne-removing external preparation, specifically a method for preparing an acne-removing cream, comprising the following steps:

[0073] 1) Each 100kg of anti-acne cream is made of the following components:

[0074] The emulsifiers are 1.6kg of steareth-2, 2.8kg of steareth-21, 2.8kg of glyceryl stearate, and 1.3kg of glyceryl stearate / PEG-100 stearate; the co-emulsifier is 2.5kg of cetearyl alcohol; the oils are 10kg of isopropyl myristate, 1kg of hydrogenated polyisobutene, and 1kg of polydimethylsiloxane, the antioxidant is 0.5kg of tocopherol acetate, the absorption promoter is 2kg of laurocapram, the anti-acne composition is 12kg, the thickener is 2kg of hydroxypropyl starch phosphate, the moisturizer is 10kg of 1,3-propylene glycol, and the balance is water.

[0075] 2) Add emulsifier, co-emulsifier and oil into the oil phase pot and heat to 88°C, keep warm for 16 minutes to dissolve the oil phase components;

[0076] 3) Add the moisturizer, thickener and water into the water phase pot and heat to 90°C, keep warm for 12 minutes to dissolve the water phase components;

[0077] 4) Under the conditions of 82°C and stirring speed of 35r / min, the oil phase component and the water phase component are successively pumped into the emulsification pot, and the homogenizer is turned on. After homogenization at 3000r / min for 4min, stirring is continued for 15min to obtain the emulsified phase component;

[0078] 5) Cool the emulsified phase component to 62° C., add antioxidant, absorption enhancer and acne-removing composition, turn on the agitator and homogenizer at the same time, homogenize at 3000 r / min for 3 min, continue stirring at 30 r / min for 10 min, cool to 43° C., and stir at 40 r / min for 18 min to obtain the acne-removing cream.

[0079] Example 2

[0080] The difference between this embodiment and embodiment 1 is that the amount of the acne-removing composition added to the acne-removing cream is different. The amount of the acne-removing composition added in this embodiment is 6.1 kg.

[0081] Comparative Example 1

[0082] The difference between this comparative example and Example 1 is that the mass ratio of Paris polyphylla, Curcuma zedoaria, Trigonum multiflorum, Bletilla striata and Prunella vulgaris is 2:2:4:7:10.

[0083] Comparative Example 2

[0084] The difference between this comparative example and Example 1 is that the mass ratio of inulinase, β-amylase, pronase and hemicellulase is 3:2:6:9.

[0085] Comparative Example 3

[0086] The difference between this comparative example and Example 1 is that the β-amylase in the composite enzymatic hydrolyzer is replaced by α-amylase, and the α-amylase is purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10002.

[0087] Comparative Example 4

[0088] The difference between this comparative example and Example 1 is that the pronase in the composite enzymatic agent is replaced by a neutral protease, and the neutral protease is purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10013.

[0089] Comparative Example 5

[0090] The difference between this comparative example and Example 1 is that the hemicellulase in the composite enzymatic agent is replaced by cellulase, and the cellulase is purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10041.

[0091] Comparative Example 6

[0092] The difference between this comparative example and Example 1 is that the first enzymolysis solution is not subjected to the ultrasound-microwave assisted enzymolysis process, the first enzymolysis solution is directly centrifuged, and the supernatant is taken and freeze-dried to obtain an enzymolysis product.

[0093] The specific preparation process of the enzymolysis product in this comparative example is:

[0094] S11: Weigh Paris polyphylla, Curcuma zedoaria, Tripterygium wilfordii, Bletilla striata and Prunella vulgaris in order according to a mass ratio of 0.6:2:4:7:10, dry at 65°C for 3h, and grind into Chinese medicine powder;

[0095] S12: adding the Chinese medicine powder to water, then adding the composite enzymolysis agent, and performing enzymolysis in a water bath at 38° C. for 1.5 h to obtain a first enzymolysis solution; the mass ratio of the Chinese medicine powder to water is 1:20, and the mass ratio of the Chinese medicine powder to the composite enzymolysis agent is 1:0.3;

[0096] The composite enzymatic hydrolyzer is inulinase, β-amylase, pronase and hemicellulase in a mass ratio of 1:2:6:9;

[0097] S13: centrifuging the first enzymatic hydrolysate, taking the supernatant, and freeze-drying it to obtain an enzymatic hydrolysate.

[0098] Comparative Example 7

[0099] The difference between this comparative example and Example 1 is that the mass ratio of Citrobacter rodinium, Bifidobacterium longum, Bacillus thuringiensis and Bacillus xylella fastidiosa is 1:2:0.7:1.

[0100] Comparative Example 8

[0101] The difference between this comparative example and Example 1 is that the Bifidobacterium longum in the composite fermentation agent is replaced by Bifidobacterium breve, and the Bifidobacterium breve is purchased from Henan Industrial Microbial Strain Engineering Technology Research Center, model number BNCC185972.

[0102] Comparative Example 9

[0103] The difference between this comparative example and Example 1 is that the Bacillus thuringiensis in the composite fermentation agent is replaced by Bacillus subtilis, and the Bacillus subtilis is purchased from Henan Industrial Microbial Strain Engineering Technology Research Center, model number BNCC338006.

[0104] Comparative Example 10

[0105] The difference between this comparative example and Example 1 is that the Xylella fastidiosa in the composite fermentation agent is replaced by Mycobacterium marinum, and Mycobacterium marinum is purchased from Henan Industrial Microbial Strain Engineering Technology Research Center, model number BNCC360708.

[0106] Comparative Example 11

[0107] The difference between this comparative example and Example 1 is that the enzymatic hydrolysis product is directly added as the acne-removing composition into the preparation process of the acne-removing cream.

[0108] Comparative Example 12

[0109] The difference between this comparative example and Example 1 is that the Chinese medicinal raw materials are not subjected to the enzymatic hydrolysis step, but are directly fermented, and the fermentation product is added as the acne-removing composition into the preparation process of the acne-removing cream.

[0110] The preparation process of the fermentation product in this comparative example is:

[0111] The fermentation steps of the enzymatic hydrolysate are:

[0112] S21: adding a composite fermentation agent to the traditional Chinese medicine powder at an inoculum amount of 8 wt %, and then adding 12 times the amount of water as the enzymatic hydrolysate, sealing, and fermenting at 30° C. for 5 days to obtain a fermentation liquid;

[0113] The composite fermentation agent includes Citrobacter acidophilus, Bifidobacterium longum, Bacillus thuringiensis and Bacillus waxyii in a mass ratio of 0.2:2:0.7:1.

[0114] S22: filtering the fermentation broth to obtain a filtrate, and sterilizing the filtrate at 110° C. for 45 minutes to inactivate the bacteria;

[0115] S23: centrifuging the sterilized filtrate, collecting the supernatant, and freeze-drying to obtain a fermentation product.

[0116] Performance Testing

[0117] (1) Human safety test

[0118] According to the human skin patch test method of the 2015 edition of the "Technical Specification for Safety of Cosmetics", the test acne cream and the negative control of each group of experiments (not containing the test substance) were patch tested, and the results were recorded according to the skin reaction grading standard (as shown in Table 1). The results are shown in Table 2.

[0119] Table 1 Classification standards for skin reactions in human trial tests

[0120]

[0121] Table 2 Safety test results

[0122]

[0123]

[0124] It can be seen from Table 2 that the acne creams prepared in the examples and comparative examples are safe and have almost no irritation to the skin, and can be used for subsequent tests of acne removal efficacy.

[0125] (2) Acne-removal efficacy test

[0126] Test method: Subjects with acne skin aged 19-28 were selected, and the test samples prepared in the embodiments and comparative examples were used once in the morning and evening every day. After 7 days, the number of acne was quantitatively tested using the Digimic2000 skin testing system, and the decrease ratio of the number of acne compared with the initial value before use was calculated. The test data results are shown in Table 3.

[0127] The ratio of decrease in the number of acne = (the number of acne before use - the number of acne after use) / the number of acne before use × 100.

[0128] Table 3 Acne removal performance test results

[0129]

[0130]

[0131] (3) Population trial survey

[0132] A population trial survey was conducted on the test products: subjects with acne skin aged 19-28 were selected, and the test products were used once in the morning and once in the evening for 7 days. The test results were statistically analyzed according to the following conditions. The statistical results are shown in Table 4.

[0133] Cured: Skin lesions disappear, or only pigmentation remains, and the efficacy index is >90%;

[0134] Significantly effective: Most of the skin lesions disappeared, with an efficacy index of 60-90%;

[0135] Effective: skin lesions partially subsided, with an efficacy index of 20-60%;

[0136] Ineffective: The skin lesions did not disappear significantly, and the efficacy index was <20%.

[0137] Table 4 Survey results

[0138]

[0139]

[0140] It can be seen from the above performance test results that the acne creams of Examples 1-2 have good acne removal effects and safety, especially Example 1 has the most outstanding comprehensive performance. This is mainly because the raw materials in the acne removal composition are reasonably matched, which improves its safety and acne removal activity as a whole.

[0141] The comparative example is not adopted the necessary technical scheme, which leads to the corresponding performance test being significantly worse than the embodiment. The ratio of various substances in the Chinese medicine raw material is changed in comparative example 1. It can be seen from the results that the acne removal effect of the acne cream prepared by it decreases, indicating that the Chinese medicine raw material in the present invention has a certain influence on the effect of improving the acne external preparation according to the proportion of compatibility. The ratio of four kinds of enzymolysis agents in the composite enzymolysis agent is changed in comparative example 2. It can be seen from the results that the acne removal effect of the acne external preparation is affected to a certain extent, indicating that the composite enzymolysis agent carries out a certain ratio of compatibility for the improvement of the comprehensive performance of the acne cream. Comparative examples 3-5 replace the β-amylase, pronase and hemicellulase in the present invention with α-amylase, neutral protease and cellulase respectively. It can be seen from the results that the acne removal effect of the acne cream decreases further, indicating that the composite enzymolysis agent of a specific type is used in the present invention to improve the comprehensive performance of the acne cream. Comparative Example 6 does not perform further ultrasound-microwave assisted enzymolysis on the first enzymolyzing agent during the enzymolysis process, but the first enzymolyzing agent is centrifuged and freeze-dried to obtain an enzymolysis product. It can be seen from the results that the acne-removing effect of the acne-removing cream is further reduced, indicating that the general enzymolysis process is first performed in the enzymolysis process of the Chinese medicine raw material in the present invention, and then the ultrasound-microwave assisted enzymolysis is performed. It has an important influence on the comprehensive performance of the acne-removing cream. The mixing ratio of the composite fermentation agent is changed in Comparative Example 7. It can be seen from the results that the comprehensive performance of the acne-removing external preparation prepared by it decreases to a certain extent, indicating that the composite fermentation agent is mixed in proportion to have a certain influence on the various properties of the acne-removing external preparation. Comparative Examples 8-9 replace Bifidobacterium longum, Bacillus thuringiensis and Bacillus waxii in the composite fermentation bacteria agent of the present invention with Bifidobacterium breve, Bacillus subtilis and Mycobacterium marinum, respectively. It can be seen from the results that the various properties of the acne-removing external preparation prepared by it are further reduced, indicating that the composite fermentation agent of a specific type is used in the present invention to improve the comprehensive performance of the acne-removing external preparation. It has a very important effect. Comparative Example 11 and Comparative Example 12 respectively use a single enzymatic hydrolysis product and a fermentation product as the acne-removing composition. From the results, it can be seen that the acne-removing effect of the acne-removing external preparation prepared therefrom is seriously reduced, indicating that the Chinese medicine raw material in the present invention is first subjected to an enzymatic hydrolysis process and then to a fermentation process. The two processes cooperate with each other to decompose and extract the active substances in the Chinese medicine raw material to the greatest extent, which is very important for improving the comprehensive performance of acne-removing products. The above experimental results further prove the importance of the technical solution defined in the present invention for its technical effect.

[0142] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An anti-acne external preparation, characterized in that: The acne-removing external preparation comprises the following raw materials in parts by weight: 5 to 15 parts of the acne-removing composition and 50 to 95 parts of auxiliary materials; The anti-acne composition is obtained by sequentially performing enzymatic hydrolysis and fermentation on Chinese medicinal raw materials; The Chinese medicinal raw materials include Paris polyphylla, Curcuma zedoaria, Trigonella ternata, Bletilla striata and Prunella vulgaris in a mass ratio of (0.5-0.8):(1-3):(3-5):(6-8):10; The enzymatic hydrolysis uses a composite enzymatic hydrolyzer, which includes inulinase, β-amylase, pronase and hemicellulase in a mass ratio of 1: (1.5-3): (5-6.5): (8-10); The fermentation uses a composite fermentation agent, which includes Citrobacter acidophilus, Bifidobacterium longum, Bacillus thuringiensis and Bacillus waxyii in a mass ratio of (0.1-0.3):(1-3):(0.5-0.8):

1.

2. The acne-removing external preparation according to claim 1, characterized in that: The enzymatic hydrolysis steps of Chinese medicine raw materials are: S11: Weigh Paris polyphylla, Curcuma zedoaria, Tripterygium wilfordii, Bletilla striata and Prunella vulgaris in order according to the mass ratio, dry at 60-80° C. for 1-5 h, and grind into Chinese medicine powder; S12: adding the Chinese medicine powder into water, then adding the composite enzymatic hydrolyzing agent, and performing enzymatic hydrolysis in a water bath at 35-40° C. for 0.5-3 h to obtain a first enzymatic hydrolyzing solution; S13: Turn on the computer microwave-ultrasonic combined catalytic synthesizer to perform ultrasonic-microwave assisted enzymolysis on the first enzymolysis solution, and then perform enzyme inactivation treatment after continuing the enzymolysis to obtain a second enzymolysis solution; S14: centrifuging the second enzymatic hydrolysate, taking the supernatant, and freeze-drying it to obtain an enzymatic hydrolysate.

3. The acne-removing external preparation according to claim 2, characterized in that: In step S12, the mass ratio of the Chinese medicine powder to water is 1:(10-30), and the mass ratio of the Chinese medicine powder to the composite enzymatic hydrolyzing agent is 1:(0.1-0.5).

4. The acne-removing external preparation according to claim 2, characterized in that: In step S13, the insertion depth of the ultrasonic probe is fixed, the microwave power is 300-800 W, the ultrasonic power is 80-120 W, the ultrasonic-microwave treatment temperature is 35-40° C., and the treatment time is 5-8 min.

5. The acne-removing external preparation according to claim 2, characterized in that: The fermentation steps of the enzymatic hydrolysate are: S21: adding a composite fermentation agent to the enzymatic hydrolyzate, then adding water, sealing, and fermenting at 28-35° C. for 3-8 days to obtain a fermentation liquid; S22: filtering the fermentation liquid to obtain a filtrate, sterilizing the filtrate to inactivate the bacteria; S23: centrifuging the sterilized filtrate, collecting the supernatant, and freeze-drying it to obtain an acne-removing composition.

6. The acne-removing external preparation according to claim 5, characterized in that: In step S21, the inoculation amount of the composite leavening agent is 5-10wt%, and the added water is 10-15 times the amount of the enzymatic hydrolysis product.

7. The acne-removing external preparation according to claim 5, characterized in that: In step S22, sterilization is performed at a temperature of 100 to 120°C for 20 to 60 minutes.

8. The acne-removing external preparation according to claim 1, characterized in that: The auxiliary material is at least one of a solvent, an emulsifier, an emulsifier aid, an antioxidant, a thickener, a preservative, a moisturizer, an emollient, a flavor, a chelating agent, and a pH adjuster.

9. The acne-removing external preparation according to claim 8, characterized in that: The solvent is at least one of water, propylene glycol, methylpropylene glycol, dipropylene glycol, pentylene glycol, and isoprene glycol; The emulsifier is at least one of glycosides, sucrose esters, amino acids, glycerides, polyglycerol esters, and polyethylene glycols; The co-emulsifier is cetearyl alcohol; The antioxidant is at least one of tocopherol, tocopherol acetate, pentaerythritol tetraester, butylated hydroxytoluene, and sodium metabisulfite; The thickener is one of xanthan gum, carrageenan, carbomer, high molecular weight cellulose, sodium polyacrylate, acrylic acid (ester) copolymer, and hydroxypropyl starch phosphate; The preservative is one of phenoxyethanol, parabens, benzoic acid and its salts, dehydroacetic acid and its salts, and sodium diacetate; The moisturizing agent is at least one of glycerin, polyglyceryl ether-26, butylene glycol, polyglyceryl-6, and 1,3-propylene glycol; The emollient is at least one of vegetable oil, synthetic oil, animal oil, and mineral oil; The chelating agent is at least one of EDTA-2Na, EDTA-3Na, and octanoylhydroxamic acid; The pH adjuster is at least one of triethanolamine, aminomethyl propanol, arginine, and inorganic bases.

10. The method for preparing an anti-acne external preparation according to any one of claims 1 to 9, characterized in that: The anti-acne composition and auxiliary materials are mixed and stirred evenly according to weight proportions to obtain an anti-acne external preparation, which is in the form of an aqueous preparation, a gel preparation or an emulsion preparation.

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