Pesticide residue scavenger and preparation method thereof

Abstract

The invention discloses a pesticide residue scavenging agent. The feed additive is prepared from the following components in parts by weight: 90-110 parts of white hyacinth beans, 9-11 parts of papain, 90-110 parts of liquorice, 45-55 parts of folium isatidis, 25-35 parts of perilla leaves, 25-35 parts of fructus forsythiae, 25-35 parts of honeysuckle, 15-25 parts of rhizoma smilacis glabrae, 15-25 parts of ginkgo leaves, 4-6 parts of soybean protease, 9-11 parts of glutathione, 5-7 parts of amino-oligosaccharin, 9-11 parts of amylase and 7-9 parts of walnut peptide. The invention also discloses a preparation method of the pesticide residue scavenger. The method has the following outstanding effects: the method is easy to operate, low in cost, popular in price and remarkable in effect, and can reach the national safety agricultural product indexes.

Description

Technical Field

[0001] The invention relates to the field of agricultural biochemistry, in particular to a pesticide residue remover and a preparation method thereof. Background Art

[0002] Existing pesticide residue removal products are mainly used to remove pesticide residues on fruits, but they cannot solve the non-point source pollution problem caused by residual hazards caused by foliar spraying, such as the pollution problem of tea, leafy vegetables and other crops. In addition, the existing corresponding products also have the disadvantages of inconvenient use, poor effect and high cost. Summary of the invention

[0003] The first purpose of the present invention is to provide a pesticide residue remover to address the deficiencies of the above-mentioned prior art and solve the problem of heavy metal pollution, harmful substances and other residues of chemical pesticides causing damage to crops and contamination of fruits after use.

[0004] Another object of the present invention is to provide a method for preparing the pesticide residue remover.

[0005] To achieve the above-mentioned purpose, the present invention is implemented as follows: a pesticide residue remover is made of the following components in parts by weight: 90-110 parts of white hyacinth bean, 9-11 parts of papain, 90-110 parts of licorice, 45-55 parts of isatis indigotica, 25-35 parts of perilla leaves, 25-35 parts of forsythia, 25-35 parts of honeysuckle, 15-25 parts of smilax glabra, 15-25 parts of ginkgo leaves, 4-6 parts of soybean protease, 9-11 parts of glutathione enzyme, 5-7 parts of amino oligosaccharide, 9-11 parts of amylase, and 7-9 parts of juglone peptide.

[0006] Preferably, the pesticide residue remover is made of the following components in parts by weight: 100 parts of white hyacinth bean, 10 parts of papain, 100 parts of licorice, 50 parts of isatis indigotica, 30 parts of perilla leaves, 30 parts of forsythia, 30 parts of honeysuckle, 20 parts of smilax glabra, 20 parts of ginkgo leaves, 5 parts of soybean protease, 10 parts of glutathione enzyme, 6 parts of amino oligosaccharides, 10 parts of amylase, and 8 parts of walnut peptide.

[0007] Wherein, the glutathionease is preferably a product with product number Q / WWTTH004052019 of Hunan Guangyi Biotechnology Co., Ltd.

[0008] The amylase is preferably a product with product number BF7658 from Xingtai Wanda Bioengineering Co., Ltd.

[0009] The walnut peptide is also walnut peptide, preferably the walnut peptide produced by Hebei Huayang Biotechnology Co., Ltd.

[0010] A method for preparing a pesticide residue remover comprises the following steps: 1. Preparation of enzymatic hydrolysis solution of white lentil 1. Take 90-110 parts of white lentils, add 180-200 parts of water and soak for 7-8 hours; 2. Pulp the soaked white lentil solution, filter and discard the residue, and collect the filtered slurry; 3. Add 800-1000 parts of water to the reactor and heat to 55-65°, then add the filtered slurry to the reactor, add 9-11 parts of papain, and stir for 1-3 hours; 4. Maintain the temperature at 55-65°, and after two hours, raise the temperature of the reactor to 90° to inactivate the enzyme for 15 minutes, and then cool it down to 40° to obtain an enzymatic solution of white lentil; 2. Preparation of Chinese herbal medicine extracts 1. Take 90-110 parts of licorice, 45-55 parts of isatis indigo leaves, 25-35 parts of perilla leaves, 25-35 parts of forsythia, 25-35 parts of honeysuckle, 15-25 parts of smilax glabra, and 15-25 parts of ginkgo leaves, crush them through a 60-100 mesh sieve, and set aside; 2. Put the above Chinese herbal medicine powder into an ultrasonic extraction tank containing 500 parts of ethanol solution with a volume ratio concentration of 50-55% for extraction. The ultrasonic frequency is 20-50 kHz, the ultrasonic power range is 100-500 W, and the extraction temperature is 40-60°C. After 30 minutes, the extract is released as the Chinese herbal medicine extract; (3) mixing the enzymatically hydrolyzed white lentil solution with the Chinese herbal medicine extract solution, stirring for 1 hour to prepare a mixed solution; (iv) adding 4-6 parts of soybean protease, 9-11 parts of glutathionease, 5-7 parts of amino oligosaccharide, 9-11 parts of amylase and 7-9 parts of juglone peptide into the mixed solution prepared in step (iii) respectively, stirring for 1 hour to obtain a final mixed solution for standby use; (v) Then add 1 part of potassium sorbate to the final mixture of (iv) and continue stirring for half an hour; (6) Packaging, warehousing, and production is completed.

[0011] The principle of the present invention is to utilize the property of white lentil protein peptide that can bind to heavy metal ions to complex heavy metals into precipitates, thereby reducing their toxicity and reducing the harm to crops and fruits; at the same time, the catalytic effect of biological enzymes is utilized to reduce the toxicity of heavy metals; and the synergistic effect of chemical groups extracted from Chinese herbal medicine extracts with detoxifying effects is used to solve the problem of heavy metal, chemical pesticide residue and other harmful substances polluting the leaves of crops.

[0012] Compared with the prior art, the present invention has the following outstanding effects: simple method of use, easy operation, low cost, affordable price, obvious effect, and can meet the national safety agricultural product indicators. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 The figure is a flow chart of the enzymatic hydrolysis process of peptides; Figure 2 Flow chart for extracting Chinese herbal medicine ingredients; Figure 3 This is a flow chart of the configuration process. DETAILED DESCRIPTION

[0014] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution of the present invention will be clearly and completely described in combination with the specific embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0015] The pesticide residue removers of Examples 1-5 are respectively prepared from the components in the following table 1 in parts by weight.

[0016]

[0017] A method for preparing a pesticide residue remover, such as Figure 1-3 As shown, the steps included are as follows: 1. Preparation of enzymatic hydrolysis solution of white lentil 1. Take white lentils, add 200 parts of water and soak for 8 hours; 2. Pulp the soaked white lentil solution, filter and discard the residue, and collect the filtered slurry; 3. Add 1000 parts of water to the reactor and heat to 60°, then add the filtered slurry to the reactor, add papain at the same time, and stir for 2 hours; 4. After maintaining at 60° for two hours, the temperature of the reactor was raised to 90° to inactivate the enzyme for 15 minutes, and then the temperature was lowered to 40° to obtain an enzymatic hydrolyzed white lentil solution; 2. Preparation of Chinese herbal medicine extracts 1. Take licorice, isatis leaf, perilla leaf, forsythia, gold and silver, smilax glabra, and ginkgo leaf, crush them through a 100-mesh sieve, and set aside; 2. Put the above Chinese herbal medicine powder into an ultrasonic extraction tank containing 500 parts of ethanol solution with a volume ratio concentration of 55% for extraction. The ultrasonic frequency is 30 kHz, the ultrasonic power range is 300 W, the extraction temperature is 50°C, and after 30 minutes, the extract is released as the Chinese herbal medicine extract; (3) mixing the enzymatically hydrolyzed white lentil solution with the Chinese herbal medicine extract solution, stirring for 1 hour to prepare a mixed solution; (iv) respectively placing soybean protease, glutathionease, amino oligosaccharide, amylase and walnut peptide into the mixed solution prepared in step (iii), stirring for 1 hour to obtain a final mixed solution for standby use; (v) Then add 1 part of potassium sorbate to the final mixture of (iv) and continue stirring for half an hour; (6) Packaging, warehousing, and production is completed.

[0018] The usage process is as follows: For crops that are harvested once, spray the leaves once 5 to 10 days before harvest, using 125 to 250 ml of scavenger and 30 to 50 kg of water per acre of land; for crops that are harvested multiple times and have been sprayed with pesticides during the harvest period, spray the pesticide residue scavenger once 3 to 5 days before each harvest, using 125 ml each time.

[0019] The mechanism of action of the present invention is as follows: Heavy metals denature proteins mainly by combining with specific groups in the proteins and destroying their spatial conformation.

[0020] Heavy metal ions include common heavy metal ions such as Hg²⁺, Cd²⁺, Pb²⁺, Cu²⁺, Ag⁺, etc., which can react with proteins.

[0021] Proteins are composed of amino acids, among which sulfhydryl, carboxyl, amino, imidazole and other related groups are binding sites for heavy metal ions. For example, Hg²⁺ easily binds to sulfhydryl groups to form stable complexes, thereby changing the structure of proteins.

[0022] When heavy metal ions bind to protein groups, the original non-covalent interactions such as hydrogen bonds, disulfide bonds, and van der Waals forces within the protein molecules will be destroyed. At the same time, new chemical bonds may be generated, causing the spatial conformation of the protein to change from an ordered, tight structure to a disordered, loose structure, and eventually denaturation, and vice versa. That is, white lentil protein liquid plays the role of complexing heavy metals.

[0023] Experiments show that when using biological enzymes to evaluate the toxicity of heavy metal ions, heavy metal ions will have a significant inhibitory effect on biological enzymes, and then the total toxicity evaluation of heavy metal ions in water samples can be achieved through enzymatic color development. For example, adding the recombinant manganese peroxidase they compiled to apple juice can degrade 95% of patulin in apple juice within 24 hours. Ultrasonic extraction of licorice, forsythia, honeysuckle, isatis indigo leaf, ginkgo leaf, smilax glabra, perilla leaf, etc., greatly reduced the content of heavy metals in the extracts. The lead content in Chinese herbal extracts dropped from 0.33μg / g to 0.03μg / g, the cadmium content dropped from 0.25μg / g to undetectable, and the mercury content dropped from 0.05μg / g to <0.01μg / g.

[0024] As described above, the pesticide residue remover of the present invention contains white lentil protein peptide, which can complex with heavy metal ions to form precipitates, thereby reducing their toxicity and reducing the harm to crops and fruits; the biological enzyme contained in the synergistic effect can further reduce the toxicity of heavy metals through the catalytic action of the biological enzyme; and the extracted Chinese herbal medicine ingredients have a detoxifying effect. The chemical groups it possesses work in synergy with the above-mentioned components to solve the problem of heavy metal, chemical pesticide residue and other harmful substances polluting the leaf surface of crops. Verification experiment

[0025] After being applied to more than 40 kinds of crops in 15 provinces of China, the comparative effect on the degradation of heavy metals and pesticide residues is quite obvious, and the following 5 test report data after use prove that the effect of this application is excellent.

[0026] Experimental Example 1: Detection of Pesticide Residues in Rice

[0027] Detection method: QD-CH-TOP050-PR-MTHD (Determination of multi-pesticide residues in plant-derived foods).

[0028] The sample is: Example 3 Testing unit: SGS-CSTC Standards Technical Services (Qingdao) Co., Ltd. Test report number: QDF21-009807-02 The test data is shown in the following table:

[0029]

[0030] Experimental Example 2--Tomato Pesticide Residue Detection Test method: Test according to BS EN 15662:2018 standard (“BS” means British standard and “EN” means European standard. This standard mainly involves food analysis - determination of pesticide residues by gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS) and related technologies.).

[0031] The sample is: Example 3 Testing unit: SGS-CSTC Standards Technical Services (Shanghai) Co., Ltd. Test report number: ASH19-035007-02 The test results are shown in the following table:

[0032] Experimental Example 3--Detection of pesticide residues in kumquat Detection method: Detection shall be carried out in accordance with BS EN 15662:2018 (LC-MS / MS&GC-MS / MS) (liquid chromatography-gas chromatography combined detection method).

[0033] The sample is: Example 3 Testing unit: SGS-CSTC Standards Technical Services (Qingdao) Co., Ltd. Test report number: QDF21-009807-02 The test results are shown in the following table:

[0034] Experimental Example 4--Detection of Pesticide Residues in Apple Test method: GB 23200.8-2016 Determination of 500 pesticides and related chemicals residues in fruits and vegetables: gas chromatography-mass spectrometry GB / T 20769-2008 Determination of 450 pesticides and related chemicals residues in fruits and vegetables: Liquid chromatography-tandem mass spectrometry The sample is: Example 3 Testing unit: SGS-CSTC Standards Technical Services Co., Ltd. Xiamen Branch Test report number: XMF23-011235-01 The test results are shown in the following table:

[0035] Experimental Example 5--Detection of pesticide residues in melon Detection method: According to GB 23200.113-2018 (GC-MS / MS) standard The sample is: Example 3 Testing unit: SGS-CSTC Standards Technical Services Co., Ltd. Dalian Branch Test report number: DLF21-008370-01 The test results are shown in the following table:

[0036]

[0037] According to the above test results, it is proved that the present invention can effectively solve the problem of damage to crops and contamination of fruits caused by the residues of harmful substances produced by chemical pesticides.

[0038] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A pesticide residue remover, characterized in that: The invention is prepared from the following components in parts by weight: 90-110 parts of white hyacinth bean, 9-11 parts of papain, 90-110 parts of liquorice, 45-55 parts of isatis indigo leaf, 25-35 parts of perilla leaf, 25-35 parts of forsythia, 25-35 parts of honeysuckle, 15-25 parts of smilax glabra, 15-25 parts of ginkgo leaf, 4-6 parts of soybean protease, 9-11 parts of glutathionease, 5-7 parts of amino oligosaccharide, 9-11 parts of amylase and 7-9 parts of juglone peptide.

2. The pesticide residue remover according to claim 1, characterized in that: The invention is prepared from the following components in parts by weight: 100 parts of white hyacinth bean, 10 parts of papain, 100 parts of liquorice, 50 parts of isatis indigo leaf, 30 parts of perilla leaf, 30 parts of forsythia, 30 parts of honeysuckle, 20 parts of smilax glabra, 20 parts of ginkgo leaf, 5 parts of soybean protease, 10 parts of glutathione enzyme, 6 parts of amino oligosaccharide, 10 parts of amylase and 8 parts of walnut peptide.

3. A method for preparing a pesticide residue remover according to any one of claims 1-2, characterized in that: The steps involved are as follows:

1. Preparation of enzymatic hydrolysis solution of white lentil 1. Take 90-110 parts of white lentils, add 180-200 parts of water and soak for 7-8 hours; 2. Pulp the soaked white lentil solution, filter and discard the residue, and collect the filtered slurry; 3. Add 800-1000 parts of water to the reactor and heat to 55-65°, then add the filtered slurry to the reactor, add 9-11 parts of papain, and stir for 1-3 hours; 4. After maintaining at 60° for two hours, the temperature of the reactor was raised to 90° to inactivate the enzyme for 15 minutes, and then the temperature was lowered to 40° to obtain an enzymatic hydrolyzed white lentil solution; 2. Preparation of Chinese herbal medicine extracts 1. Take 90-110 parts of licorice, 45-55 parts of isatis indigo leaves, 25-35 parts of perilla leaves, 25-35 parts of forsythia, 25-35 parts of honeysuckle, 15-25 parts of smilax glabra, and 15-25 parts of ginkgo leaves, and crush them; 2. Put the above Chinese herbal medicine powder into an ultrasonic extraction tank containing 500 parts of ethanol solution for extraction. The ultrasonic frequency is 20-50kHz, the ultrasonic power range is 100-500W, and the extraction temperature is 40-60°C. After 30 minutes, the extract is released as the Chinese herbal medicine extract; (3) mixing the enzymatically hydrolyzed white lentil solution with the Chinese herbal medicine extract solution, stirring for 1 hour to prepare a mixed solution; (iv) adding 4-6 parts of soybean protease, 9-11 parts of glutathionease, 5-7 parts of amino oligosaccharide, 9-11 parts of amylase and 7-9 parts of juglone peptide into the mixed solution prepared in step (iii) respectively, stirring for 1 hour to obtain a final mixed solution for standby use; (v) Then add 1 part of potassium sorbate to the final mixture of (iv) and continue stirring for half an hour.

4. The method for preparing a pesticide residue remover according to claim 3, characterized in that: During the preparation of the Chinese herbal medicine extract, the extract is crushed through a 60-100 mesh sieve.

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