384 results about "Tanshinone IIA" patented technology

The invention is concerned with a kind of extract of total ketone of salviae miltiorrhizae and total phenolic acid and its produce method form radix Salviae Miltiorrhizae. The extract of total ketone of salviae miltiorrhizae has cryptotanshinone, tanshinone I, tanshinone IIA, methyl Tanshinon, dihydrotanshinon I and ramification. The extract of total phenolic acid has salvianolic acid A, salvianolic acid B, protocatechuic aldehyde and ramification. The extract can be got form one or arbitrary compound of extraction with solvent method, macroporous resin method, column chromatography and liquid-liquid counter-current chromatography. The summation of the content to each total ketone of salviae miltiorrhizae is 20 to 100 percnte (w / w) of the extract of total ketone of salviae miltiorrhizae, the contene of cryptotanshinone, tanshinone I and tanshinone IIA is 5 to 100 percent (w / w) of whole content of total ketone of salviae miltiorrhizae. The summation of the content to each total phenolic acid is 5 to 100 percent (w / w) of the extract of the radix salviae miltiorrhizae total phenolic acid. The content of salvianolic acid B is the 5 to 100 percent (w / w) of the whole salvianolic acid.

The invention relates to a method for extracting tanshinone which comprises the following two steps: first, alcohol extraction process: separating the extraction liquid and dregs with the danshen liposolubility content from danshen medicine materical crude slices or disintegrated danshen medicinal materials by bath method, hypersonic extraction or microwave extraction in condition of normal temperature or heating, and reserving, adding correct concentration alcohol into the dregs, inverse flow extracting with heating to get the extraction, which combined with the extraction get from the first extraction, removing the solid impurities by the methods of filtration, centrifugation or deposition, and reserving. Second, isolation method separation: adding the medicine liquid get from the said alcohol extraction process to a macroreticular resinous adsorbent column, gradient eluting by alcohol with a finite concentration respectively to get the extraction I (with cryptotanshinone and dihydrotanshinone I) and extraction II (with tanshinone IIA and tanshinone I).

A composition of natural herb extract for treating cardiovascular disease or vascular disorder and its method of preparation and separation, and particularly to a composition prepared from natural herb extract comprising constituents of Danshensu, Tanshinone IIA, matrine, oxymatrine and puerarin for lowering the risk factors of heart disease or treating the heart disease that side effects are minimized. The working ranges of Danshensu, Tanshinone IIA, matrine, oxymatrine and puerarin are between 5 and 40 mg / kg per day and a ratio of Danshensu, Tanshinone IIA, matrine, oxymatrine, and puerarin is 1:1:1:1:1. The extraction and separation method of the five active ingredients are also illustrated. The composition can be exhibit in different forms for administration.

The invention discloses an endophytic fungus in salvia miltiorrhiza bunge. The endophytic fungus is isolated and obtained from the living body of salvia miltiorrhiza bunge by adopting the endophytic fungus isolation and purification technology and is identified to be trichoderma atroviride through microbial taxonomy. The strain was collected in the China General Microbiological Culture Collection Center, with collection number being CGMCC No.4712.. Tanshinone compounds tanshinone I and tanshinone IIA are generated through liquid fermentation of the strain of the endophytic fungus in salvia miltiorrhiza bunge, so the endophytic fungus is an important microorganism for finding the new resources such as the tanshinone chemical components tanshinone I and tanshinone IIA.

The invention relates to tanshinone compounds in the technical field of medicine and applications thereof. The tanshinone compounds contain tanshinone I, tanshinone IIA, cryptotanshinone and dihydrotanshinone I and the structural formulas are shown as below. The method of the invention uses the tanshinone compounds as the active ingredients of the androgen receptor antagonist; the compounds are used alone or used along with the excipient to prepare oral agents or non-oral agents for curing the diseases related to androgen.

The invention relates to a DanShen fingerprint spectrum quality control method, comprising that (1), adding 1.0g DanShen powder into carbinol to extract via microwave, (2), washing flow phase gradient that the Alltima C18 column is 4.6mm, 250mm, and 5mum, checking wavelength is 280nm, flow speed is 1.0ml / min, the column temperature is 20-40Deg. C, and the sample amount is 10-20ul, (3), building standard fingerprint spectrum that the first peak is DanShen element, the eighth peak is rosmarinic acid, the ninth peak is alkannic acid, the tenth peak is phenolic acid B, the eleventh peak is phenolic acid B isomer, the fourteenth peak is cryptotanshinone and the fourteenth peak is tanshinone IIA, (4), controlling the quality of fingerprint spectrum that the check peak relative holding times are 0.21 of DanShen element, 0.91 of rosmarinic acid, 0.93 of alkannic acid, 1.00 of phenolic acid B, 1.04 of phenolic acid B isomer, 0.92 of cryptotanshinone and 1.00 of tanshinone IIA, (5), the DanShen planting collecting method. The invention can control the quality of materials to assure the stable quality of product.

The invention provides an ultrafine wall-breaking grinding method for salviae miltiorrhizae, and belongs to the field of traditional Chinese medicines. The ultrafine wall-breaking grinding method for salviae miltiorrhizae provided by the invention comprises the following steps: (1) conventionally crushing salviae miltiorrhizae, and filtering through an 80-100mesh sieve to obtain salviae miltiorrhizae powder; (2) mixing sorbitol with phenylalanine evenly, wherein the mass ratio of the sorbitol to the phenylalanine preferably is 1 to 1, and obtaining sorbitol-phenylalanine mixed powder; and (3) mixing the salviae miltiorrhizae powder with the sorbitol-phenylalanine mixed powder evenly, wherein the mass ratio of the salviae miltiorrhizae powder to the sorbitol-phenylalanine mixed powder preferably is (200-100) to 1, crushing the powder until the mean grain size is smaller than 2 microns by an ultrafine grinder, so as to obtain ultrafine salviae miltiorrhizae wall-broken powder. Compared with a conventional ultrafine wall-breaking grinding method, the ultrafine wall-breaking grinding method for salviae miltiorrhizae provided by the invention has the advantages of short processing time, high powder extraction rate, high retention rate and dissolution rate of tanshinone IIA and salvianolic acid B, and small mean grain size of powder.

The invention discloses a gene for generating related diterpene synthase together with a tanshinone compound as well as an encoding product and an application thereof. The gene is named as the tanshin ent-kaurene synthase gene (SmKSL) and is obtained from tanshin by cloning and adopting a gene chip technology, and as for the sequence, see to SEQ ID No.1. The protein encoded by the SmKSL is the protein provided with an aminoacid residue sequence with SEQ ID No.2 or is the protein derived via the SEQ ID No. 2 by replacement, deletion or addition of one or more aminoacid residues for the aminoacid residue sequence with SEQ ID No.2 and provided with the same activity with the aminoacid residue sequence with SEQ ID No.2. The SmKSL is a key enzyme gene in a tanshin diterpene secondary metabolic pathway, the genetic expression is closely related with the generation of the tanshinone compounds, for example, tanshinone IIA, and the invention has important theoretical and practical significances for adjusting and producing plant diterpenoid compounds and cultivating the excellent medical new plant variety.

The invention provides a method for simultaneously detecting main components of paeoniflorin, beta ecdysterone, laetrile, mulberroside A, caffeic acid, ferulic acid, salvianolic acid B, astragaloside, formononetin, cryptotanshinone and tanshinone IIA of a Naoxintong capsule in a plasma sample by a liquid chromatography-tandem mass spectrometry (HPLC-MS / MS). In a liquid chromatogram, a mobile phase consists of acetonitrile and a formic acid aqueous solution of which the volume fraction is 0.1%, and gradient elution is used. A mass spectrum uses a quick positive and negative ions switching and analyzing mode and an MRM (Multiple Reaction Monitoring) scanning manner. After the Naoxintong capsule is taken, the situations of the changes of the blood-medicine concentration of several kinds of main components in the plasma of a rat are detected at the same time. The methodological survey results indicate that the established method conforms to determination requirements on biological samples in a body; the method is good in sensitivity, high in specificity, stable, reliable, and suitable for detecting substances with lower contents.

The invention discloses a tanshinone II A microemulsion preparation which has targeting and comparatively high entrapment efficiency and drug-loading rate, is not haemolytic and mainly applied to the treatment of tumor and a preparation method thereof. The invention comprises the following raw materials by weight percentage: 0.6-0.8 percent of tanshinone II A, 0.6-1.8 percent of phosphotide for injection, 2.5-3 percent of glycerin for injection, 1.5-3 percent of F68 for injection, 11-15 percent of ethyl oleate and water for injection. The preparation method of the invention is that first the tanshinone II A is dissolved in the ethyl oleate to prepare the oil phase mixture solution; next the phosphotide for injection, the glycerin for injection and the F68 for injection are dissolved in the water for injection which accounts for 60-70percent of the total water for injection to prepare the water phase solution; then the oil phase solution and the water phase solution are respectively preheated to 60-70DEG C and the oil phase solution is slowly dropped to the water phase solution when the water phase solution is blended; the mixed solution is then sheared for 1-2minutes in a high shearing dispersing emulsification machine and filtrated with a 0.8Mum filtration membrane when the solution is still hot and the rest water for injection is added to the filtrate; the liquid obtained from step d is homogenized by a high pressure emulsification machine and next filtrated with the 0.8 Mum filtration membrane; finally the filtrate obtained from step e is packaged, filled with nitrogen, sealed and disinfected.

Disclosed is a medicinal composition for treating senile dementia, wherein 1g of the dried active sites comprises tanshinone IIA 1.79-9.01mg, salvianolic acid B 35.87-225.23mg, danshensu 4.93-45.05mg, protocatechuic aldehyde 0.10-4.50mg, and notoginseng saponin R 13.14-22.50mg.

The invention relates to an antibacterial tanshinone extract extracted from Salvia spp. roots, a preparation method thereof, application of the antibacterial tanshinone extract and products of the antibacterial tanshinone extract. The extract contains cryptotanshinone, dihydro-tanshinone, tanshinone I and tanshinone IIA, wherein the total weight content of the four compounds is 40 to 99 percent of the total weight content of the extract; the weight content of the cryptotanshinone is 50 to 95 percent of the total weight of the four compounds; the weight content of the dihydro-tanshinone is 5 to 30 percent of the total weight of the four compounds; the weight content of the tanshinone I is 0 to 20 percent of the total weight of the four compounds; and the weight content of the tanshinone IIA is 0 to 40 percent of the total weight of the four compounds. The preparation method comprises the steps of diacolation extraction and purification for two times. The extract is used for preparing antibacterial medicines or other products. The tanshinone extract obtained has high purity, more reasonable proportions of various compositions, and can exert better clinic effect.

The invention provides a method for refining a molecularly imprinted polymer. The method comprises the following steps of: mixing tanshinone IIA serving as a template, a functional monomer, a cross-linking agent, an initiator and a pore-forming agent to prepare a homogeneous system; performing polymerization under the protection of nitrogen; grinding the prepared material into powder and sieving; and soaking, washing and eluting the powder with an alcohol solvent to obtain the molecularly imprinted polymer. The molecularly imprinted polymer prepared by the method represents high affinity and selectivity on the tanshinone IIA in an aqueous mobile phase, serves as a specific separating material and has a wide application prospect on separation, enrichment and purification of the tanshinone IIA in the Chinese medicament. In the method, materials are mixed in one step during synthesis; the preparation process is obviously simplified; time is saved; and an aqueous phase elution system is adopted, so that the application range is enlarged and the elution effect is obvious. The tanshinone IIA provided by the invention can serve as a solid phase extraction material to separate and purify the tanshinone IIA from a salvia miltiorrhiza bunge ethanol crude extract.

Generally, tanshinone IIA, cryptotanshinone, dihydro tanshinone and tanshinone I are obtained through column chromatography, which has too lower content of effective component in the product, and is time consuming and material consuming. The present invention provides tanshinone IIA, cryptotanshinone, dihydro tanshinone and tanshinone I extracting process including column chromatography and simultaneous extraction with ethyl acetate, silica gel H, benzene, chloroform and 60-90# petroleum ether, and the extracting process has effectively raised content of effective component in the product.

The invention discloses a tanshinone IIA derivative. A connecting arm, phosphocreatine and an analogue of the phosphocreatine form a conjugate of which the structure is as shown in formula (I) in the specification, wherein n is equal to 1-3, m is equal to 0-3, R1 and R2 respectively independently represent hydrogen atoms, C1-C5 alkyl, cycloalkyl, vinyl and alkynyl, the R1 and the R2 also can form ternary to hexatomic rings, and M represents metal ions, like sodium ions. The compound can be used for improving the water solubility of the whole molecule through sodium phosphate in the phosphocreatine on the premise of maintaining the activity of the tanshinone IIA; moreover, a phosphocreatine fragment in the molecule can play a dual protection effect on myocardial muscles.

The invention relates to a preparation method of chemical reference standard of tanshinone IIA, which adopts 50-97 percent of tanshinone IIA by weight as raw material, preparative high performance liquid chromatography as a separation method and a certain ratio of methanol water solution as an elution system to produce the tanshinone IIA chemical reference standard, the purity of which is more than 98 percent. The process steps of the invention comprise: sample pretreatment, chromatographic column treatment, sample injection, elution, on-line monitor, collecting eluriant containing tanshinone IIA and decompression concentration tanshinone IIA to be dried; after the steps, the chemical reference standard of the tanshinone IIA is obtained, which has been monitored by high efficiency liquid chromatography, the content of tanshinone IIA can reach more than 98 percent.

A quality control method for the Chinese medicine to treat thrombus features that a thin-layer chromatography is used for the identification to earthworm, vomiting nut, musk, corydalis tuber, toad, liquorice root, etc and an efficient liquid-phase chromatography is used to measure the contents of tanshinone IIA and strychnine.

A process for extracting and refining tanshinone IIA by supercritical CO2 includes loading pwodered red sage root in extractor, extracting at 32-80 deg.C and 30-45 MPa, rectifying the supercritical CO2 fluid in which the extract is dissolved at 25-75 deg.C and 7.5-32 MPa, and separating at 15-75 deg.C and 6.5-25 MPa. Its advantages are high purity up to 80-95% and output rate up to 90% or more, not degradating easily and cyclic use of CO2.

The invention relates to a traditional Chinese medicine composition for treating insomnia and a preparation method and a quality control method thereof. The medicine composition comprises mother-of-pearl, Polygonum multiform, glossy privet fruit, Salvia miltiorrhiza and other bulk medicines. The preparation method is easy, simple and convenient, and can preserve effective components in the medicines and give play to the treatment effect of the medicines to the maximum degree. The quality control method comprises the steps of thin-layer identification of the Salvia miltiorrhiza, Schisandra chinensis, prepared rehmannia rhizome, the glossy privet fruit and Silktree Albizzia barks, content inspection of an ethanol extract, and content assay of an effective component tanshinone IIA in the medicines, and can effectively control the quality of the medicines and guarantee the stability of the treatment effect of the medicines.

The invention provides a method for using a near infrared spectroscopy to measure contents of multiple components in a radix et rhizoma salviae miltiorrhizae medicinal material and application feasibility of the method in a Chinese patent medicine raw material quality assurance system is discussed. The method comprises the steps: utilizing a high performance liquid chromatography to measure contents of danshinolic acid B, rosmarinic acid, alkannic acid, tanshinone IIA, tanshinone I and cryptotanshinone in radix et rhizoma salviae miltiorrhizae as contrast values; utilizing near infrared diffusion reflection method to collect a sample spectrum, comparing different spectrum pretreating methods and modeling effects of modeling waveband and optimizing modeling parameters; utilizing a partial least squares (PLS) to respectively establish near infrared quantitative analysis models of danshinolic acid B, rosmarinic acid, alkannic acid and tanshinone components (tanshinone IIA plus tanshinoneI plus cryptotanshinone) in the radix et rhizoma salviae miltiorrhizae and evaluating the models. The quantitative analysis models established by the method disclosed by the invention can meet a requirement of quickly obtaining chemical information of massive radix et rhizoma salviae miltiorrhizae medicinal material samples.

The invention is applicable to the field of medical chemistry and provides a natural and edible composition with healthcare functions, application of composition, makeup cosmetics and a preparation method of the makeup cosmetics. The composition comprises multiple active molecular tanshinone IIA, a ginseng extract and soybean lecithin which are natural, all the components have an effect synergistic superposition function, have significant therapeutic effects, are natural, edible, safe and free of side effects, can be applied to preparation of makeup cosmetics with multi-molecular, multi-target, multi-layer, multi-link and multi-way functions, including but not limited to lipsticks, lip cream, lip gloss, colored lip cream, lip balm, lip moisturizing gel, lip protection oil, lip glaze, lip dying liquids, lip liners, rouge, eye shadow, blusher, pressed powder, liquid foundation, BB cream, air cushion cream, eye liner, eye shadow cream, eyebrow pencils and finishing powder, and can endow the cosmetics with multiple skin care and healthcare effects so as to enrich functions of the cosmetics.

A tanshinone IIAsodium sulfonate injection in the form of powder is prepared from tanshinone IIA sodium sulfonate (50-100 wt.%) and medical auxiliaries (0-50 wt.%) including skeleton supporting agent and antioxidizing agent.

The invention discloses a tanshinone IIA-polyactic acid / hydroxyacetic acid microsphere and a preparation method thereof. The microsphere is prepared by drying oil-in-water type emulsion, wherein the oil phase is dichloromethane solution of a polyactic acid / hydroxyacetic acid copolymer and the water phase is the water solution of polyvinyl alcohol. The drug content of tanshinone IIA in the microsphere is 1-10% and the entrapment efficiency is 60-90%. The particle size range of the microsphere is 30-200mm. The tanshinone IIA-polyactic acid / hydroxyacetic acid microsphere provided by the invention is suitable for interventional therapy of liver cancers, has a good liver tumor peripheral vascular thrombosis function, has an effective thrombosis time of 7-60 days, can be distributed in tumor tissues in a targeted manner, slowly release drugs, increase the local concentrations of the drugs, prolong the drug metabolism time, obviously inhibit animal liver tumor growth and prolong the animal lifetime and can inhibit expressions of a human hypoxia inducible factor 1alpha and a vascular endothelial growth factor and reduce the tumor tissue microvessel density after thrombosis.

The invention relates to the use of tanshinone IIA in the preparation of medicaments for treating atherosclerosis, myocardial infarction, coronary heart disease, tumor, purulency osteomyelitis and tonsillitis. The tanshinone IIA of the invention can be prepared into various dosage types.

A composition comprising tanshinone compounds selected from the group consisting of miltirone, 1,2-didehydromiltirone, tanshinone IIA, tanshinone I and dihydrotanshinone I isolated from Salviae miltiorrhizae Bunge showing potent inhibiting effect on the aggregation and toxicity of beta-amyloid and recovering activity of memory learning disorder confirmed by Y maze study and Passive Avoidance study. Therefore it can be used as the therapeutics or health food for treating and preventing cognitive function disorder with safe.

The invention relates to a Chinese herbal medicine compound preparation for treating skin and mucous membrane disease. The Chinese herbal medicine compound preparation is prepared from the following raw materials: cortex moutan, polygonum chinense, radix salviae miltiorrhizae, herba lycopi, common dayflower herb, rhizoma smilacis glabrae, ramulus cinnamomi, caulis dendrobii officinalis, gorgon fruit and cortex cinnamomi; the ingredient of the Chinese herbal medicine compound preparation comprises a cortex moutan extract, a polygonum chinense extract, a radix salviae miltiorrhizae extract, a herba lycopi extract, a common dayflower herb extract, a rhizoma smilacis glabrae extract, a ramulus cinnamomi extract, a caulis dendrobii officinalis extract, a gorgon fruit extract and a cortex cinnamomi extract; and the effective ingredient of the cortex moutan extract is paeonol, the effective ingredient of the polygonum chinense extract is quercitrin, the effective ingredient of the radix salviae miltiorrhizae extract is tanshinone IIA, the effective ingredient of herba lycopi is ursolic acid, the effective ingredient of common dayflower herb is commelinin, the effective ingredient of rhizoma smilacis glabrae is astilbin, the effective ingredient of ramulus cinnamomi is cinnamaldehyde, the effective ingredient of caulis dendrobii officinalis is erianin, the effective ingredient of gorgon fruit is cyclo(profilum), and the effective ingredient of cortex cinnamomi is cinnamaldehyde.

The invention relates to tanshinone IIA derivatives. The structure of the tanshinone IIA derivatives are carbonylated and acylated products of tanshinone IIA furan ring 2-position sulfonamide as show in a formula (I), wherein R is C1-6 alkyl, cycloalkyl, alkenyl, alkynyl, aryl and aromatic heterocycle and M is a metal ion such as a sodium salt. According to the compounds, on the basis that the activity and water solubility of sodium tanshinone IIA sulfonate are maintained, the acidity of the compounds is reduced; when sodium tanshinone IIA sulfonate is injected, the stimulation to patients is reduced and the stability of the compounds is improved.

The invention provides a drug composition for treating cardio-cerebrovascular diseases and a preparation method. The drug composition is jointly formed by such active ingredients as 2-10 parts by weight of salvianolic acid B, 0.1-0.5 part by weight of tanshinone IIA, 0.1-0.5 part by weight of ferulic acid, 0.4-2 parts by weight of ligustilide and 2-10 parts by weight of puerarin and the auxiliary added ingredients acceptable in the drugs. Tests show that the drug composition can obviously reduce brain tissue hemorrhagic necrosis caused by brain ischemia reperfusion injury and alleviate injuries of nerve cells and nerve fibers.

The invention provides a preparation method of a tanshinone extract and a water soluble substance thereof. The preparation method comprises the following steps of: extracting tanshinone by alcohol with the concentration being greater than or equal to 80%, wherein the tanshinone is segmented tanshinone or a complete material, and the mass ratio of the alcohol to the tanshinone is (5-20):1, heating and carrying out refluxing extraction for 2-5 times, combining and filtrating to obtain an extracting solution, wherein the extraction lasts for 0.25-3h every time; carrying out vacuum concentration on the extracting solution till an extract without the alcohol taste is obtained; and washing the extract by water for 5-12 times and drying under reduced pressure to obtain the tanshinone extract which contains 10.0-15.0% of tanshinone IIA and 8.0-11.0% of cryptotanshinone. The tanshinone extract prepared by the method provided by the invention has the advantages that the content of active substances is high, and the solubility of the water soluble substance of the tanshinone extract is good.

The solution of sample is prepared according to following steps. Powder of red sange root 0.3 g is fetched with methanol 50ml being added. Then, ultrasound operation is carried out for the solution just prepared. Cooling the preparing solution to room temperature and shaking up obtains the solution of sample. Reference substance is prepared according to following steps. fetching an amount of tanshinone IIA with methanol being added produces the solution of reference substance (20 ª–g / ml). The standard finger print of fat soluble component of red sange root is obtained through measurement and calculation by using high performance liquid chromatograph for 5 ª–L of sample solution and 5 ª–L of reference substance. The method provides the advantages of simple, stable, high accuracy and good repeatability. Thus, the invention can be used to control quality and distinguish whether the product is true or false.