103 results about "Nerolidol" patented technology

The invention provides a sweet taste functional clustering substance preparation which has the taste close to tobacco sweet taste and is capable of obviously increasing the tobacco taste, which comprises the following compositions in mass percentage: 0.0001 to 0.0004 percent of clove oil, 0.0001 to 0.0006 percent of anise oil, 0.2 to 0.8 percent of date oil, 0.01 to 0.03 percent of maltol, 0.05 to 0.15 percent of dihydroactinidiolide, 0.002 to 0.008 percent of benzyl cinnamate, 0.1 to 0.6 percent of gamma-Valerolactone, 0.002 to 0.008 percent of phenethyl acetate, 0.0001 to 0.0003 percent of menthyl acetate, 0.0001 to 0.0006 percent of Beta-Damascenone, 0.02 to 0.08 percent of methyl cyclopentyl hydroxybenzyl thiocyanide ketone, 0.003 to 0.009 percent of dihydrogen Beta-Damascenone ketene, 0.001 to 0.003 percent of raspberry ketone, 0.01 to 0.05 percent of neohesperidin, 0.03 to 0.09 percent of vanilline, 0.001 to 0.005 percent of perillartine, 0.01 to 0.03 percent of aspartame, 0.02 to 0.08 percent of glycyrrhizine, 0.01 to 0.03 percent of malic acid, 0.002 to 0.008 percent of 2- methylbutanoic acid, 0.02 to 0.08 percent of sodium glutamate, 0.01 to 0.05 percent of sodium chloride, 0.002 to 0.006 percent of 2-phenylethanol, 0.0001 to 0.0003 percent of geraniol, 0.002 to 0.008 percent of nerolidol, 0.5 to 1.5 percent of guarana extractive, 1.0 to 2.0 percent of tomato extractive, 0.1 to 0.3 percent of grape extractive, and 95 percent of ethanol residue. The addition of the spice preparation can greatly improve the tobacco quality of cigarettes, and increase rich and inviting sweet taste of the cigarettes, thereby reducing the use level of sound tobacco. Moreover, the product has stable quality and good economic benefits.

The invention provides a composition and application of the composition in cigarettes and particularly provides a composition. The composition contains a main flavor enhancer, a coordination agent, a modifier and a fixative, wherein the main flavor enhancer is an extract of a preliminary tea product, the coordination agent is tomato ketone, linalool, geraniol, dihydrogen jasmone, geranyl acetate or any combination thereof, the modifier is caryophyllene oxide, nerolidol, beta-damascenone, megastigmatrienone, beta-ionone, dihydro kiwifruit lactone or any combination thereof, and the fixative is a ladanum extract, a Yunyan tobacco extract or a combination thereof. The composition has an elegant and natural aroma, is fresh and fragrant, has a sweet, fragrant and graceful smell and can be widely used in the fields of tobaccos, foods and daily chemicals and the like.

The invention relates to a method for machining tea, and in particular relates to a method for machining flower-fragrance black tea in a low-temperature compounding mode. The method comprises the following steps: spreading to cool high-fragrance type oolong tea with two to three fresh leaves on one bud as a raw material, finely manipulating the green tea leaves, kneading, thinly spreading, fermenting at high temperature and low humidity, and drying so as to obtain coarse tea; appropriately selecting to remove peduncles and pieces, highlighting the fragrance and thus accomplishing the machining. According to the flower-fragrance black tea machined by using the method, the weight loss of the green leaves in the machining process can be controlled, and the machining time is shortened, particularly under poor weather conditions; fragrant substances such as flower and fruit fragrance of alcohols, alkenes, esters and ketones are added, and the content of benzyl cyanide with pungent odor, indole and main fragrant substances, namely, nerolidol, in oolong tea, are increased, so that the fragrance and the taste quality of the tea are improved, and then the quality of the black tea is improved. Sensory evaluation on the finished tea shows that the finished tea is fat, strong, compact, black and tender in appearance, tea tips are visible, the fragrance is strong and heavy, the fragrance of flower and fruit appears, the taste is fresh and mellow, the bottom of a cup has the flower fragrance, and the quality is superior to that of black tea manufactured by using the conventional method.

The invention discloses an application of nerol in preparing a banana flower thrip inducing agent. Nerol is active as active component of the inducing agent. When the inducing agent is used for inducing the banana flower thrip, the volume percentage concentration of nerol is 10-20%. An experiment of inducing the banana flower thrip through a Y-shaped tube shows that when the inducing agent is a paraffin oil solution of nerol which is 10-20% in volume percentage concentration, an average inducing rate on banana flower thrip adult insects is 90.00-76.67%; and in a field induction experiment, within 10d of treating through the paraffin oil solution of nerol which is 10% in volume percentage concentration, the inducing effect on the banana flower thrip adult insects is remarkably higher than that on banana bud, yellow sticky colored card and water. Nerol can be used for indicating occurrence and occurrence quantity of banana flower thrip in advanced in bud stage, and indicating to use an insecticide to prevent and control the banana flower thrip in the field.

The invention discloses a terpenoid synthase for producing nerolidol and application thereof, and belongs to the field of synthetic biology. Terpene synthase TPF09930 for producing the nerolidol is provided, a nucleotide sequence of the terpene synthase TPF09930 is as shown in SEQIDNO:2, the terpene synthase TPF09930 is combined with mevalonic acid pathway related genes to construct a strain for producing the nerolidol. By overexpression of Escherichia coli XL1 blue-derived atoB gene or idi gene of mevalonic acid pathway, catalytic substrate Farnesyl pyrophosphate can be synthesized in large scale, and producing of the nerolidol can be further promoted. The terpene synthase has specificity and high efficiency, can improve the nerolidol yield, greatly overcomes the defects of a large amount of raw materials and a low nerolidol yield, reduces study cost, and ensures greenness and environmental friendliness.

The invention discloses a tripterygium wilfordii diterpene synthase TwGES1 and an encoding gene and application thereof. Twges1 genes are obtained through cloning from tripterygium wilfordii suspension cells; and the genes are key enzyme genes obtained through synthesis of diterpene components from tripterygium wilfordii. Experiments prove that TwGES1 proteins can catalyze GGPP to form geranyl linalool ((E, E)-geranyl linalool), can catalyze FPP to form nerolidol ((E)-nerolidol), not only have important significance on biological synthesis of such diterpene compounds as triptolide in the tripterygium wilfordii and but also have important theoretical and practical significance on adjustment and production of plant diterpene compounds and cultivation of high-quality tripterygium wilfordii.

The invention discloses saccharomyces cerevisiae capable of auto-synthesizing terpenoid substances and applications thereof, belonging to the field of fermentation engineering. The saccharomyces cerevisiae capable of auto-synthesizing various terpenoid substances denovo is screened from fermented grains of white wine. The stain is preserved in the general microbiology centre in China on September 4, 2013, and the preservation number is CGMCC No.8130. The strain is subjected to liquid-state or solid-state fermented cultivation so as to generate ten kinds of terpenoid substances including linalool, alpha-terpineol, citronellol, myrcenol, nerolidol, alpha-dragosantol, farnesol, alpha-farnesene, beta-farnesene and bisabolene. The cerevisiae strain is added in the wine making process and can improve the flavor of white wine and increase the nutritive value of white wine.

The invention discloses a processing method of orchid-flavored black tea. The processing method comprises the following steps: (1) picking fresh tea leaves each with one bud and three to four leaves or tea leaves each with buds on a sunny day or in a rainy-to-fine weather; (2) cooling the picked fresh tea leaves, rocking twice and spreading twice; (3) rocking for the second time and spreading for a long time; (4) rocking the fresh tea leaves for the third time in the next day; (5) rocking for the third time and continuously spreading; (6) withering the continuously spread fresh leaves by sunlight, performing fixation, and rolling while the fresh leaves are hot; (7) primarily baking the rolled leaves, spreading, sunning, and performing pile fermentation; (8) fully drying and warehousing. According to the processing method, the orchid aromas in the tea leaves are excited to the maximum extent by controlling the picking time and mastering the fresh leaf maturity, alternatively spreading and rocking for multiple times and withering by sunlight, so that substances such as amino acid and nerolidol influencing the aromas and sweet and fresh tastes of orchids in the tea leaves are brought into play to the maximum extent, and then the black tea prepared by adopting the method has remarkable orchid aromas and is fresh, sweet and honeyed in taste.

The invention discloses a preparation method of ginkgo leaf lipoid components having antibacterial activities. Ginkgo leaf which is a raw material is extracted with a non-polar solvent, and undergoes molecular distillation separation, column chromatography, re-crystallization and purification to obtain eight compounds (sequentially comprising isophytol, nerolidol, linalool, beta-sitosterol acetate, beta-sitosterol, stigmasterol, ergosterol and daucosterol), polypentenol, ginkgo leaf lipoid total unsaponifiable matters, ginkgo leaf lipoid crystals, a ginkgo leaf lipoid light fraction and a ginkgo leaf lipoid heavy friction. The eight compounds, polypentenol, the ginkgo leaf lipoid total unsaponifiable matters, the ginkgo leaf lipoid crystals, the ginkgo leaf lipoid light fraction and the ginkgo leaf lipoid heavy friction have inhibition effects on salmonellas, Staphylococcus aureus and Aspergillus niger.

The invention relates to a CYP450 gene participating in DMNT and TMTT biosynthesis and a coding product and application of the CYP450 gene, and belongs to the field of plant molecular biology. The protein has amino acid sequences shown as SEQ ID NO.2. Experiments prove that the PlCYP82 protein obtained in the mode that the obtained cDNA nucleotide sequences and PlCYP82 coding protein sequences of lima beans PlCYP82 are expressed in yeast WAT21 cells has the activity of catalyzing (E)-nerolidol and (E,E)-geranyl linalool; PlCYP82 gene-transferred arabidopsis thaliana is obtained through a floral-dip method, and a result shows that PlCYP82 gene-transferred arabidopsis thaliana plants can significantly attract female cotesia plutellae.

The invention discloses a myliataylori sesquiterpene synthetase MTa and a gene sequence thereof. The gene is 1,383 bp in full length and encodes 460 amino acids, the molecular weight of enzyme proteinis 52.6 KDa, pESC-LEU yeast protein expression plasmids are adopted as carriers, and Saccharomyces cerevisiae WQ011 is adopted as a host, so that heterogenous expression of the myliataylori sesquiterpene synthetase MTa is achieved. A recombinant strain WQ011 / pESC-LEU-MTa can simultaneously produce two sesquiterpene alcohols, namely farnesol and nerolidol. The yields of nerolidol and farnesol in a50-mL shake flask are 1.03 mg / L and 2.00 mg / L respectively. Acquisition of the gene has profound significance on the research of sesquiterpene compounds of moss plants.

The invention discloses common threewingnut root sesquiterpene synthase TwNES as well as a coding gene and application thereof. According to the common threewingnut root sesquiterpene synthase TwNES as well as the coding gene and application thereof disclosed by the invention, a Twnes gene is obtained by cloning common threewingnut root suspension cells, and this gene is a key enzyme gene which is acquired from common threewingnut root for the first time and is synthesized by sesquiterpenoids components. Proved by experiments, TwNES protein disclosed by the invention not only can catalyze FPP to form nerolidol ((E)-nerolidol), but also can catalyze GGPP to form geranyl linalool ((E, E)-geranyllinalool), plays an important role in the synthesis of sesquiterpenoids alkaloids in common threewingnut root, and also has important theoretical and practical significance in the adjustment and production of plant sesquiterpenoids compounds and in the cultivation of high-quality common threewingnut root.

This invention relates to synthetic method of a ( E, E) - geranyl linalool. The invention takes (E) - nerolidol as raw material. The hydroxyl is shield by dihydropyrane, gain ( E) - nerolidol tetrahydropyrane aether; selenium dioxide and teri-butyl hydroperoxide selectively oxidize the anti-form methyl of ( E) - nerolidol tetrahydropyrane aether to gain anti-form allyl position hydroxylated oxidative product ( E, E) - 12 - hydroxy nerolidol tetrahydropyrane aether, transit halogenating reaction to gain ( E, E) - 12 - halogeno- nerolidol tetrahydropyrane aether, then take reaction with isopropyl methyl ketone that is selectively divested one proton by diisopropyl amido lithium, generate ( 6E, 10E) - 2, 6, 10, 14 - tetramethyl - 14 - ( tetrahydropyrane - 2 - oxygen) -16 - 6, 10, 15 - triene - 3 - ketone, use sodium borohydride to reduce to gain ( 6E, 10E) - 2, 6, 10, 14 - tetramethyl - 14 - ( tetrahydropyrane - 2 - oxygen) -16 - 6, 10, 15 - triene - 3 - alcohol, takes reaction with sulfonyl chloride or sulphonic acid ester with alkali presence to gain ( 6E, 10E) - 2, 6, 10, 14 - tetramethyl - 14 - ( tetrahydropyrane - 2 oxygen) -16 - 6, 10, 15 - triene - 3 - alcoholic sulphonic acid ester, then divide sulphonic acid ester group under base catalysis to gain ( E, E) - geranyl linalool tetrahydropyrane aether, and by deprotection to gain ( E, E) - geranyl linalool. ˕For the configuration of ( E) - nerolidol 3 position tertiary carbon is not influenced in the course of reaction, if use ( E) - nerolidol that has optical activity as raw material, should gain optical active ( E, E) - geranyl linalool. ((E, E)-geranyl linalool can replace Teprenone and such type medicament intermediate, natural product intermediate, insect pheromone and spice etc.

Provided is a method for producing farnesal that is useful as an intermediate for the production of a medicinal agent, an agrichemical and a flavoring agent. Specifically provided is a method for producing farnesal (3), characterized by reacting (E)-nerolidol (1) with an oxidizing agent in the presence of a vanadium complex represented by general formula (2) (wherein R1 to R7 are as defined in the description and claims).

The invention discloses a method for increasing the yield of nerolidol of saccharomyces cerevisiae. According to the method, in the saccharomyces cerevisiae whose Gal80 gene is knocked out, a Gal promoter is used for driving the expression of a mevalonate pathway metabolizing enzyme and a nerolidol synthase to form a secondary growth induction system, and meanwhile a transcription factor hac1 geneis overexpressed to construct a yeast engineered strain for synthesizing nerolidol. In the method, a yeast secondary growth induction system is used for metabolic transformation; when a large numberof expression cassettes driven by the GAL promoter are integrated on a genome, the transcriptional activity of the GAL promoter is greatly reduced; however, an overexpressed transcription factor Hac1in the method can increase the transcriptional activity and strain viability of the GAL promoter and accordingly promote the synthesis of the target metabolite product in the secondary growth induction system; the yield of nerolidol is increased by 48.4% to 497 mg / L.

The invention discloses a new preparation method for a gefarnate key intermediate (4E, 8E)-5,9,13-trimethyl-4,8,12-tetradecyl triolefinic acid, and belongs to medicines. The method comprises the following steps of: reacting nerolidol with hydrogen chloride to obtain an intermediate (III) and then condensing with diethyl malonate to obtain an intermediate (IV); then hydrolyzing and acidifying to obtain an intermediate (V); and finally removing a carboxylic acid to obtain the gefarnate key intermediate. In the preparation method, methanol with high toxicity and combustible and explosive diethyl ether used in the prior art are effectively avoided in a production process, and the safety is enhanced; and the method has the advantages of mild reaction conditions, easiness and convenience in operation and high suitability for industrial production. The prepared gefarnate key intermediate is not required to be purified and can be directly prepared into the gefarnate with geraniol through a method well known by those of skill in the art; and the purity can reach over 99.5 percent.

This invention discloses an aroma-producing compound from Cymbidium goeringii and the extraction method thereof. The compound comprises octadecatrienoic aldehyde,nerolidol,dibutylphthalate,heptacosane,pentadecanoic acid,pentadecanoic acid,hexadecanoic acid(palmic acid),oleic acid,linolic acid and cyclohexadecane. The aroma-producing compound is extracted from the flowers of Cymbidium goeringii by the following steps: leaching and filtering, reflux leaching, filtering, combined seperation, volatilized speration, concentration and drying. The main discovery is the octadecatrienoic aldehyde, a critical aroma-producing substance. Besides, the extraction rate of the aroma-producing substances is pretty high, with the content upto 30% by using octadecatrienoic aldehyde and nerolidol as the major aroma-producing product. The components of this invention has satisfied the basic requirements as natural flavoring, and can be used in flavoring agents, perfume, scent emitting objects and bath articles, allowing this natural perfume for industrial application.

The invention relates to a processing method of an oolong tea. The processing method comprises the following steps of picking fresh leaves, withering the pickled leaves at low temperature through a roller, rocking withered leaves through a comprehensive leaf rocking barrel, airing the rocked leaves, performing fixation on the aired leaves, wrapping and twisting the leaves after fixation, baking the twisted leaves, frying the baked leaves in a pot for shaping, wrapping and twisting the shaped leaves once again, and roasting the twisted leaves in the pot. Withering the pickled fresh leaves at the low temperature through a 110 type roller has the advantages of (I) promoting a series of low-boiling-point leaf alcohol and the like in the fresh leaves, which have green grass ingredients, to partly dissipate so as to facilitate the increase of fragrance components including hexenoic acid and the like; (II) promoting a series of nerolidol and the like in the fresh leaves, including aromatic substances, to accelerate a biochemical reaction so as to facilitate the decomposition, the transformation and the accumulation of fragrance components and substances and improve the fragrance of finished tea products; and (III) solving the actual difficulty that the Sun is not available on rainy days. The wrapping and twisting times, and the re-wrapping and re-twisting times decreased reduced, so that the fragrance and the brewing resistant degree of the finished tea products are improved, and the crumb rate of processed leaves is substantially reduced. The leaves are fried in the pot for shaping and are roasted in the pot, so that the color, the fragrance, the taste and the shape of the finished tea products are guaranteed, the crumb teas can be substantially decreased, and the production cost is obviously reduced.

The invention discloses the preparation of a ruthenium-modified Ir / C catalyst and its application in citral hydrogenation. The catalyst includes an activated carbon carrier and active components are Ir nanoparticles and Ru nanoparticles. The loading amount of Ir nanoparticles is 1.0-5.0%, and the loading amount of Ru nanoparticles is 0-4.0%. The invention also discloses a preparation method of the ruthenium-iridium / C catalyst. First, the activated carbon is treated with nitric acid, and then the active component iridium and the additive ruthenium are loaded to obtain a catalyst precursor, which is dried and reduced. The catalyst of the present invention can replace part of Ir with cheaper Ru, and at the same time has higher catalytic activity and selectivity, and the catalyst can be used multiple times, reducing production costs. The catalyst of the present invention is used to hydrogenate citral to synthesize nerol. The raw material conversion rate is 100%, the selectivity of nerolidol / geraniol reaches 98.0%, and the reaction process conditions are mild, which can fully meet the requirements of industrial production.

The invention discloses application of novel loquat leaf sesquiterpene glycoside in relieving liver cell lipid deposition, and also discloses application in preparation of drugs and health-care foodsfor preventing or treating non-alcoholic fatty liver. The name of the sesquiterpene glycoside is nerolidol-3-O-alpha-L-arabinopyranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1-> 2)-[alpha-L-rhamnopyranosyl-(1-> 6)]-beta-D-glucopyranoside.

The invention discloses jasmine essence for a blasting bead for a cigarette and a preparation method and application of the jasmine essence. The jasmine essence is prepared from, by mass, 10-20% of benzyl acetate, 2-10% of jasmonic acid methyl ester, 10-20% of Jasmine lactone, 3-6% of nerolidol, 15-25% of linalool, 10-20% of jasmine and 10-15% of benzyl alcohol. The cigarette with jasmine flower fragrance is prepared, raw materials required for preparing the jasmine essence are separated from extract of natural jasmine products and obtained, and the defect that synthetic essence only has the shape same with a natural perfume material and no essence of the natural perfume material is overcome. Through separation of a column chromatography technology, the mixed odor influence of jasmine extract and macromolecule substances of pectin, protein, fiber and the like in the essence on the sensory quality of the cigarette is reduced. The prepared blasting bead can reduce stimulation of the cigarette, mixed odor of the cigarette is reduced, the comfort degree of the cigarette is improved, and the cigarette has the jasmine fragrance characteristic.

The invention provides an efficient and rapid lignum dalbergiae odoriferae essential oil extracting method. The method includes the steps of S1, conducting water boiling, wherein lignum dalbergiae odoriferae heartwood is pulverized into powder, and the powder is placed in a cloth bag to be boiled in water; S2, conducting fermenting, wherein after water boiling, the lignum dalbergiae odoriferae heartwood is kept in water for soaking and fermenting, fermenting is conducted for 3-12 h after the temperature is lowered to 50-60 DEG C, fermenting is conducted for 1-3 h after the temperature is lowered to 30-37 DEG C, and then fermenting is conducted for 1-3 h at the temperature of 28 DEG C; S3, conducting distilling, wherein the lignum dalbergiae odoriferae essential oil is extracted through a waterproof distilling method for 6 h or longer. The lignum dalbergiae odoriferae essential oil prepared through the method is high in yield, the extraction yield is increased by 35% or above, the content of active components is high, especially the content of trans-nerolidol is remarkably increased, it is shown that the yield and quality of the lignum dalbergiae odoriferae essential oil are remarkably increased by means of the extracting method, and the essential oil has higher value.

The fragrance composition of Citrus flowers according to the present invention is produced by respectively adding jasmone and beta-caryophyllene to linalool, myrcene, nerolidol, methyl anthranilate or the like which have been analysed to be aromatic components of the flowers of Citrus unshin and the flowers of Citrus natsudaidai by means of the SPME method, and by adding gamma-terpinene to linalool, myrcene, geraniol, beta-ocimene or the like which have been analysed to be aromatic components of the flowers of Citrus sudachi, and also adding alpha-farnesene to beta-ocimene, 6-methyl-5-hepten-2-one, anthranilaldehyde, limonene, methyl anthranilate or the like which have been analysed to be aromatic components of the flowers of Citrus japonica. The present invention makes it possible to provide a fragrance composition which is outstandingly palatable while reproducing the inherent aromas of Citrus unshin, Citrus natsudaidai, Citrus sudachi and Citrus japonica.

The invention belongs to the technical field of biological prevention and control of field pests, and discloses an attractant for adults of monolepta hieroglyphica. The active components of the attractant is composed of a host plant volatile matter of the adults of monolepta hieroglyphica. The host plant volatile matter is prepared from 1-heptene, orange alkene, trans-2-hexenal, alpha-farnesene, trans-2-hexene-1-ol, leaf alcohol, alpha-phellandrene, beta-pinene and nerolidol; The attractant is reasonable in proportion and remarkable in attraction effect, has the advantages of being green, safe, effective, long in lasting period and the like, has important significance on protection of field crops such as cotton and corn which are damaged by the monolepta hieroglyphica, and can be widely applied to actually-produced farmlands and urban green belts.

The invention discloses a spice of a heat-not-burn cigarette, a preparation method and the heat-not-burn cigarette. The spice comprises glucoside, and aglycone of the glucoside is selected from one orat least two of eugenol, vanitrope, isoeugenol, beta-phenethyl alcohol, nerolidol, geraniol and hydroxycitronellol. Glycoside obtained by taking aroma substances containing alcoholic hydroxyl group or phenolic hydroxyl group, like eugenol and vanitrope, as aglycone is used as essence. Compared with single use of aroma substances, volatilization of the aroma substances can be remarkably reduced due to the fact that the aroma substances exist in the form of glucoside, durability is guaranteed, and stability is improved. The glycosyl does not influence the original flavor of the aroma substances, but combines the inherent flavor of the aroma substances through the inherent flavor of the glycosyl, so that the compounded aroma is stronger, good aroma smelling feeling and smoking feeling are provided, and the spice is suitable for being applied to heat-not-burn cigarettes.

The fragrance composition of Citrus flowers according to the present invention is produced by respectively adding jasmone and β-caryophyllene to linalool, myrcene, nerolidol, methyl anthranilate or the like which have been analyzed to be aromatic components of the flowers of Citrus unshin and the flowers of Citrus natsudaidai by means of the SPME method, and by adding γ-terpinene to linalool, myrcene, geraniol, β-ocimene or the like which have been analyzed to be aromatic components of the flowers of Citrus sudachi, and also adding α-farnesene to β-ocimene, 6-methyl-5-hepten-2-one, anthranilaldehyde, limonene, methyl anthranilate or the like which have been analyzed to be aromatic components of the flowers of Citrus japonica. The present invention makes it possible to provide a fragrance composition which is outstandingly palatable while reproducing the inherent aromas of Citrus unshin, Citrus natsudaidai, Citrus sudachi and Citrus japonica.

The invention discloses mylia taylori gray sesquiterpene synthetase MTc and a gene sequence thereof. The gene is 1032 bp in length and encodes 343 amino acids. The molecular weight of the enzyme protein is 39.5KDa. The pESC-LEU yeast protein expression plasmid is used as the vector, and Saccharomyces cerevisiae WQ011 is used as the host. Heterologous expression of the mylia taylori gray sesquiterpene synthetase MTc is realized. The recombinant strain WQ011 / pESC-LEU-MTc can simultaneously produce two sesquiterpene alcohols, namely farnesol and nerolidol. The yield of a 50 mL shake flask is 19.88 mg / L of nerolidol and 4.81 mg / L of farnesol. The acquisition of the gene has far-reaching significance for the study of sesquiterpenoid in moss plants.

The invention belongs to the technical field of pharmacy and discloses application of nerolidol in preparing drug for treating fungal keratitis. It is put forward for the first time that nerolidol caninhibit fungus growth and has treatment effect on fungal keratitis in vivo and in vitro. In in-vivo experiments, C57BL / 6 mice are adopted to build a fungal keratitis model to study impact of nerolidol on clinical scoring of model mouse cornea, neutrophil collection and human oxidized low-density lipoprotein receptor 1 and interleukin 1beta expression. In in-vitro experiments, inhibiting effect ofnerolidol at different concentrations on growth of aspergillus fumigatus is studied, and a human corneal epithelial cell model stimulated by aspergillus fumigatus is adopted to verify that nerolidolhas impact on LOX-1 / IL-1beta signal pathway.

Provided is a method for treating conditions and / or symptoms associated with a stressful event, comprising administering to a subject in need thereof a therapeutically effective amount of a composition comprising at least one cannabinoid; at least one terpene selected from the group consisting of Pinene, Myrcene, Caryophyllene, Humulene, Nerolidol, Bisabolol, Linalool, Fenchol, Limonene, Terpinene, Terpineol, Eucalyptol, geraniol and combinations thereof, wherein said at least two terpenes are present in the composition at a concentration of at least about 40 wt %, based on the weight of the total amount of terpenes in the composition and at least 5% by weight of a non-cannabinoid, non-terpene carrier.