270 results about "Terpenoid" patented technology

The invention discloses a phoebe zhennan PzGGPS12 gene and application of the phoebe zhennan PzGGPS12 gene to improvement of yield and drought tolerance of plant terpenoids, and belongs to the field of genetic engineering, a key enzyme gene PzGGPS12 for catalyzing biosynthesis of the terpenoids in phoebe zhennan wood is screened and analyzed, and a full-length CDS nucleic acid sequence of the PzGGPS12 is obtained. Transgenic experiments prove that overexpression of the gene improves the relative content of tobacco monoterpenes and triterpenes. Short-term drought experiments prove that overexpression of the gene can promote biosynthesis of sesquiterpenes, diterpenes and triterpenes so as to enhance drought tolerance of transgenic tobacco. Therefore, the gene can be introduced into a plant as a target gene, the yield of terpenoids in the plant is increased, and the drought tolerance of the plant is enhanced so as to improve the variety of the plant.

The invention discloses saccharomyces cerevisiae recombinant bacteria for efficiently producing terpenoids as well as a construction method and application of the saccharomyces cerevisiae recombinant bacteria. According to the construction method, saccharomyces cerevisiae rich in terpenoid precursor farnesyl pyrophosphate is taken as a starting strain, the GAL80 protein level in a galactose induction system is regulated and controlled by introducing an N-Degron strategy, and meanwhile, a glucose concentration response type promoter HXT1 is introduced to effectively regulate and control the expression of GAL80 from the transcriptional level; the saccharomyces cerevisiae recombinant strain for efficiently producing the terpenoids can be obtained. The strategy is applied to a saccharomyces cerevisiae engineering strain of beta-elemene and patchouli alcohol, and a remarkable effect is achieved. And in the high-density fermentation process of the saccharomyces cerevisiae, the yield of the beta-elemene is obviously increased and reaches 4818.6 mg / L. The invention provides a solid research basis for sustainable biosynthesis of terpenoids, and has a wide application prospect.

The invention discloses a saccharomyces cerevisiae recombinant strain based on a uracil metabolism gene URA3 as well as a construction method and application of the saccharomyces cerevisiae recombinant strain. The construction method comprises the following steps: taking saccharomyces cerevisiae rich in farnesyl pyrophosphate as an original strain, introducing a uracil metabolism gene URA3, synchronously overexpressing a ribosome biosynthetic gene UTP10 and an iron metabolism gene FIT3, and utilizing the uracil metabolism gene URA3 to express up-regulation correlation with the ribosome biosynthetic gene UTP10 and the iron metabolism gene FIT3, so as to obtain the farnesyl pyrophosphate-rich saccharomyces cerevisiae. The metabolic flux of the terpenoids is enhanced, and the saccharomyces cerevisiae recombinant strain for efficiently producing the terpenoids is constructed. According to the method, a metabolic network of yeast is systematically optimized, and combined regulation and control on precursor flux distribution, protein translation capability and metal ion steady state are realized, so that the microbial synthesis efficiency of sesquiterpenoids such as patchouli alcohol and beta-elemene is remarkably improved.

The invention relates to the technical field of gene engineering, in particular to a grape transcription factor VvNAC53, application of the grape transcription factor VvNAC53 and a method for regulating and controlling the content of terpenoids in grapes. The nucleotide sequence for coding the grape transcription factor VvNAC53 is as shown in SEQ ID NO. 1; the content of terpenoids in grapes can be regulated and controlled by adopting VvNAC53; the regulation and control method comprises the following steps: transferring a grape transcription factor VvNAC53 into grapes and performing overexpression to reduce the content of terpenoids in grape fruits so as to directionally cultivate grape germplasm with different aroma types.

A method for preparing nanoparticles of triterpenoid small molecules co-assembled with Ce6 and copper ions wrapped in cancer cell membranes and its application. The present invention prepares a pure natural co-assembled nanotransmission system with new morphological characteristics and multiple biological activities - terpenoid compound and photosensitizer co-assembled nanoparticles. In the composite nanoassembly, triterpenoid compounds and photosensitizer Ce6 are assembled into nanoparticles, providing effective and safe chemotherapy and photodynamic therapy. Subsequently, Cu is complexed on the surface of the nanoparticles. 2+ , when Cu is introduced 2+ When activated, the reduced PDT effect caused by excessive glutathione in the tumor consuming reactive oxygen species can be counteracted. Furthermore, it can enhance CDT therapy through a Fenton-like catalytic reaction with overexpressed H₂O₂ at the tumor site. Finally, these molecules are encapsulated by the tumor cell membrane. By constructing CM@OABACe6 / CuNPs with homologous targeting, a triple synergistic platform for cancer treatment using PDT, chemotherapy, and CDT has been created.

Described are liquid compositions that contain a desired (e.g., extracted) plant material such as cannabinoid, terpene, terpenoid, or the like, contained, e.g., dissolved, suspended, or emulsified, in the liquid, which contains surfactant; methods of preparing these types of liquid compositions; and methods of processing this type of liquid composition to collect, isolate, concentrate, or purify a desired target material contained in the liquid composition.

A method of plant extraction comprising: (a) providing a plurality of trichomes from a plant at a first temperature below about 35 °C; and either: (b1) applying a negative pressure to the trichomes to volatize at least one desired compound from the trichomes to form a gaseous plant extract; and (c1) condensing the gaseous plant extract at a second temperature below about 25 °C to form a liquid plant extract; or (b2) combining the trichomes with a fluid to form a mixture; (c2) inducing cavitation in the mixture to release at least one desired compound from the trichome into the fluid; and (d2) separating out the at least one desired compound from the mixture; wherein the at least one desired compound is a terpene, a terpenoid, a cannabinoid, or a flavonoid.

The invention discloses a composition for reversing skin cell aging and application thereof, and relates to the technical field of cosmetics. The invention provides a composition. The composition comprises a component A and a component B, the component A comprises a camellia nitidissima extract, a camellia yunnanensis flower extract and a catechu extract; and the component B is oat beta-glucan. When the camellia nitidissima extract, the camellia yunnanensis flower extract and the catechu extract cooperate with the oat beta-glucan, small-molecule terpenoids and the like in the extracts can be used as natural penetration enhancers to form molecular channels with the oat beta-glucan; meanwhile, the components are synergistic, so that the removal of senescent cells can be enhanced, the cell regeneration is further enhanced, the cutin is effectively removed, the skin is brightened, and the mild anti-aging and brightening effects on the sensitive skin are achieved.

The invention belongs to the technical field of microbial fermentation, and relates to bacillus cereus capable of biologically converting limonene, the bacillus cereus is preserved in the China Center for Culture Collection, the classification name is Bacillus cereus GJ-02, the preservation number is CCTCC M 20251438, and the preservation date is June 23, 2025; according to the invention, the bacillus cereus strain capable of biologically converting limonene and having efficient conversion capability is separated and screened, a new strain resource is provided for microbial conversion of D-limonene, and a theoretical basis is provided for biosynthesis and metabolic engineering modification of terpenoids; therefore, the method has a wide application prospect in the field of microbial fermentation.

The invention belongs to the technical field of microbial fermentation, and relates to a nitrate reducing pseudomonas capable of biologically converting limonene, which is preserved in the China Center for Culture Collection, the classification name of which is Pseudomonas nitritiducens GJ-01, the preservation number of which is CCTCC M 20251439, and the preservation date of which is June 23, 2025, and the preservation number of which is CCTCC M 20251439. According to the invention, the nitrate reducing pseudomonas strain with efficient conversion capability and capable of biologically converting limonene is separated and screened, so that a new strain resource is provided for microbial conversion of D-limonene, and a theoretical basis is provided for biosynthesis and metabolic engineering modification of terpenoids; therefore, the method has a wide application prospect in the field of microbial fermentation.

The invention provides a strain of saccharomyces cerevisiae and application of the saccharomyces cerevisiae. The preservation number of the Filobasidium magnum is CGMCC (China General Microbiological Culture Collection Center) No.34231, and the Filobasidium magnum secretes lipoxygenase, protease and amylase. Compared with a control group fermented by sterile water, the saccharomyces cerevisiae fermentation group has the advantages that when the saccharomyces cerevisiae fermentation group is applied to the enhanced fermentation of the tobacco leaves, the contents of carotenoid degradation products, cembrane degradation products, Maillard reaction products, chlorophyll degradation products, terpenoid substances and the like of the tobacco leaves can be remarkably increased, the potassium-chlorine ratio can be increased by 50.1%, and the yield of the tobacco leaves can be increased by 50.1%. The total content of aroma components of the tobacco leaves is increased by 37.7%. According to the invention, the aroma amount, aroma richness, maturity, sweetness and combustibility of cigar tobacco leaves can be obviously improved, irritation and offensive odor are reduced, the aroma characteristics of sweet aroma, costustoot, bean aroma and the like are highlighted, and the fermentation period is shortened to 15 days.

The invention provides a collection and detection method for electronic cigarette atomized released substances, and belongs to the technical field of electronic cigarette detection. The method comprises the following steps: step 1, adsorbing and collecting aerosol release substances generated after atomization of electronic cigarette atomized liquid by using a solid-phase extraction column, wherein the solid-phase extraction column is filled with an adsorbing material polyethylene divinylbenzene; step 2, eluting the solid-phase extraction column after adsorption in the step 1 to obtain an eluent as a to-be-detected sample solution; and 3, detecting the content of a sweetening agent in the to-be-detected sample solution obtained in the step 2 by using a liquid chromatograph, and detecting the content of a cooling agent and a flavor substance in the to-be-detected sample solution by using a gas chromatograph-mass spectrometer. The method provided by the invention has very good trapping efficiency on substances with various phase states and pH values in aerosol release substances of the electronic cigarette, especially has a relatively strong adsorption effect on low-carbon ester and terpene flavor substances, improves the detection accuracy, and is suitable for simultaneous detection of various components in electronic cigarette products.

The invention relates to the technical field of gene engineering, in particular to a kit and a method for increasing the content of terpenoids in grapes. According to the method, the kit is adopted, the grape transcription factor MYB1R1 is transferred into grapes and overexpressed, and the content of terpenoids in grape fruits is increased. According to the method disclosed by the invention, the MYB1R1 gene is over-expressed in grapes, so that grape plants with higher terpenoids content compared with wild types can be obtained, and the content of partial terpenoids in grape peels can be effectively increased, so that grape germplasm with rich flower fragrance can be cultivated; the method plays a positive role in improving the flower and fruit fragrance of varieties, cultivating new varieties and accelerating the breeding process.

The invention discloses a compound fermentation method of acid-resistant probiotics and application of the acid-resistant probiotics in blueberry juice, and particularly relates to compound fermentation of plant lactobacillus B4 and plant lactobacillus SS6 and application of the compound fermentation method. The two strains are preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation numbers of the strains are respectively CGMCC NO.30881 and CGMCC NO.30880. The invention also discloses a preparation method of the strain. The viable count of the lactobacillus plantarum still exceeds 6 log CFU / ml after the lactobacillus plantarum is fermented for 72 hours in blueberry juice (pH = 3.0), and is two orders of magnitude higher than that of a common commercial strain (lactobacillus plantarum LP39); and the two strains are subjected to compound fermentation, so that the total phenol content, DPPH free radical scavenging rate and terpenoid content of the blueberry juice can be increased at the same time, and the influence of fermentation on the color of the blueberry juice can be reduced.

The invention relates to the technical field of gene engineering, in particular to an atractylodes macrocephala terpene synthase gene AmTPS9 and a product coded by the same and application of the gene AmTPS9. The gene AmTPS9 has one of the following nucleotide sequences: (1) a nucleotide sequence as shown in SEQ ID NO.1; and (2) a nucleotide sequence which is obtained by substituting, deleting or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID NO.1 and expresses protein with the same function. According to the invention, a coding gene of an atractylodes macrocephala terpene synthase gene (AmTPS) is cloned from the rhizome of the atractylodes macrocephala, and the synthase can be applied to a pathway prepared by taking farnesyl pyrophosphate (FPP) as a substrate. By utilizing the gene provided by the invention, the content of terpenoids in bighead atractylodes rhizome can be increased through a genetic engineering technology.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a promoter of a pinus massoniana terpene synthase gene Pm TPS (-)-alpha-pinene and application of the promoter. According to the invention, a promoter fragment of a Pm TPS (-)-alpha-pinene gene and three homeopathic action elements with jasmonic acid response on the promoter fragment are obtained from pinus massoniana. A complete promoter fragment of the Pm TPS (-)-alpha-pinene gene of the pinus massoniana is transformed into arabidopsis thaliana by means of agrobacterium mediation, and the promoter can drive expression of a GUS gene in leaves of the arabidopsis thaliana or nicotiana benthamiana. GUS staining and GUS enzyme activity of transgenic Bensi tobacco leaves are reduced along with deletion of jasmonic acid response cis-acting elements on the promoter. Furthermore, the yield of pine resin terpenoids can be increased by utilizing the promoter and the homeopathic element thereof.

The invention discloses a ginkgo natural extract copolymerized antibacterial conductive gel as well as a preparation method and application thereof. The conductive gel is prepared from the following raw materials in parts by mass: 1 to 10 parts of ginkgo natural extract, 1 to 8 parts of imidazolyl alkenyl amic acid compound, 0.02 to 0.1 part of stabilizer, 0.01 to 0.2 part of emulsifier, 0.5 to 1 part of organic silicon cross-linking agent, 1 to 5 parts of modifier, 0.05 to 0.3 part of organic oxidant, 1 to 10 parts of natural polysaccharide, 15 to 25 parts of organic solvent and 10 to 30 parts of water. The gingko natural extract has an antibacterial effect, micro-current is conducted by the gingko natural extract through a macromolecular chain and aromatic continuous conjugated structures in flavonoids and olefinic terpenoids, the antibacterial and conductive gel which is low in toxicity and can be applied to organism tissue repair is prepared, and the gel serving as a flexible medium can conduct electric signals and also can be used for repairing tissues of organisms. The foreign matter reaction risk can be reduced through the antibacterial function, meanwhile, the toxicity of metal ion leakage is avoided, and the method has unique advantages in the micro-current related fields such as flexible electronic sensors and bioelectrical stimulation dressings.

The invention relates to a recombinant genetically engineered bacterium for synthesizing terpenoids. The recombinant genetically engineered bacterium is saccharomyces cerevisiae in which a gal80 gene, an lpp1 gene and a dpp1 gene are knocked out and a heterologous MVA pathway gene and an AuoblA gene are integrated. According to the invention, a ophiobollin synthesis route is introduced into the saccharomyces cerevisiae in a heterologous manner for the first time, so that de novo synthesis of a ophiobollin skeleton compound, namely the ophiobollin F and an oxidation product of the ophiobollin F is realized; the synthesis of the ophiobolin F of 5.1 g / L is realized, which is also the highest sesterterpene heterologous synthesis yield reported at present; according to the invention, the ophiobolin 5-OH-21-CHO-opF with a new structure, which has excellent anti-cancer activity, is obtained through identification; the engineering strain of high-yield ophiobolin U is constructed, and the shake flask yield reaches 128.9 mg / L.

The present invention discloses terpene compounds derived from polar fungi, their preparation methods and applications, and respectively provides a calamenane-type sesquiterpene compound eutyacorane and a pimarane-type diterpene compound libertellenone Z derived from polar fungi. The structures are shown in the following formulas I and II respectively: The experimental results show that the two compounds have a better inhibition rate on the release of NO in the LPS-induced inflammatory model of mouse macrophages RAW264.7, providing a new type of choice for anti-inflammatory drugs.

The present disclosure relates to methods of making cyclic terpenoids through substrate-controlled catalysis taking place in the presence of a supramolecular capsule.

The invention provides a liquid-cooled micro-channel copper cold plate surface treatment liquid and a treatment method. The treating fluid adopts a mixed water extract of malus melliana leaves, cassia tree leaves and lemon peels, the weak acid property of the mixed water extract has an activation effect on the micro-channel copper cold plate, and a large number of substances such as flavonoids, phenols and terpenes which have a corrosion inhibition effect on copper in the water extract can be promoted to be stably adsorbed on the surface of the micro-channel copper cold plate in a nanocluster form; the stability of the cold plate is improved, meanwhile, the microcosmic area of the cold plate is increased, and the heat dissipation effect is improved. In addition, the wettability of the cooling liquid on the micro-channel copper cold plate can be rapidly improved through the plasma treatment step of the surface treatment method, and therefore the heat dissipation effect of the micro-channel copper cold plate is further improved. Therefore, the processed micro-channel copper cold plate is high in heat dissipation efficiency and good in stability. The treatment method disclosed by the invention is simple, convenient, rapid and efficient, has the characteristics of low cost, environment friendliness, high operation automation degree, easiness in large-scale industrialization and the like, and has a wide application prospect.

The invention discloses a key enzyme farnesyl pyrophosphate synthase of a black fungus terpenoid synthetic route, and belongs to the technical field of protein. The invention aims to provide a key enzyme gene of a mevalonic acid pathway synthesis pathway in black fungus. The invention provides an FPPS protein of black fungus. The amino acid sequence of the FPPS protein is as shown in SEQ ID NO. 2. A solid foundation is laid for deeply exploring functions of key genes for triterpene synthesis, and meanwhile important data support is provided for systematically explaining specific effects of the genes in terpene synthesis routes.

This invention relates to the use of a natural terpene compound in pharmaceuticals, said natural terpene compound having the structure shown in formula (I), and having the function of preparing a compound for the prevention or treatment of hypoxia-inducible factor-1. α Use in medicines for diseases associated with abnormally elevated activity. This invention has discovered that the compound possesses hypoxia-inducible factor-1. α It exhibits inhibitory activity, effectively suppressing macrophage inflammatory responses and hepatocyte damage. It also demonstrates significant protective function against lipopolysaccharide / galactosamine-induced acute liver injury in mice, and can be used as a lead compound for the preparation of new drugs or drugs for the prevention and treatment of acute liver injury.

The invention provides a Victoria veitinia yeast and an application of the Victoria veitinia yeast in preparation of tobacco products. The Victoria yeast victoriae SC-13 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 16, 2025, and the preservation number of the Victoria yeast victoriae SC-13 is CGMCC No. 34237. The invention further discloses a preparation method of the Victoria yeast victoriae SC-13. The strain is used for enhanced fermentation of tobacco leaves, the content of carotenoid degradation products, Maillard reaction products, chlorophyll degradation products and terpenoid substances can be remarkably increased, meanwhile, smoke irritation is remarkably reduced, and cleanliness is improved. In addition, the strain can increase the ratio of potassium ions to chloride ions, so that the combustion performance of the tobacco leaves is greatly improved, and the problems of easy flameout, insufficient combustion and the like of the tobacco leaves in a traditional fermentation process are solved.

The invention discloses a method for catalytically synthesizing a terpenoid perfume raw material by using dehydrogenase, the dehydrogenase ADH1 is derived from Klebsiella HNYJ-1, and the method comprises the following three steps: firstly, obtaining ADH1 through fermentation culture, centrifugal crushing, salting-out and column chromatography purification; then ADH1 is mixed with acrylamide, nano magnetic Fe3O4 and the like, and ADH1 gel magnetic beads are prepared; and finally, constructing a reaction system containing substrates (carvone, geraniol and the like), ADH1 gel magnetic beads and coenzyme NADP < + >, reacting at 40 DEG C, and eluting to collect terpene perfume materials such as carvone, geranial and the like. The method has the outstanding advantages that ADH1 is high in catalytic efficiency, high in specificity and few in by-product; the safety risk of chemical catalysis is avoided by a biological enzyme method, and the product belongs to natural perfume; the process can be amplified in equal proportion and is suitable for industrial production; when the product is added to cigarettes, the aroma can be remarkably improved, offensive odor and irritation are reduced, and the application value is high.

There is provided a recombinant cell comprising a plasmid expressing terpenoid synthase gene and one or more modification to the gene of the recombinant cell. Also provided is a recombinant cell for use in biosynthesis and a method of producing isoprenoid / terpenoid.

The invention discloses a peroxisome positioning signal peptide and application thereof. The invention relates to an application of an N-terminal II-type peroxisome positioning signal peptide alone or in combination with a C-terminal I-type signal peptide in the biosynthesis of a compartment high-value compound. The invention comprises the construction of an auxiliary carrier of two peroxisome signal peptides, and covers the application of the auxiliary carrier in the biosynthesis of terpene substances such as limonene, linalool and patchouli alcohol, and flavonoid substances such as daidzein, genistein, 3-hydracrylic acid and coenzyme Q10. The peroxisome signal peptide disclosed by the invention can provide a new element for metabolic engineering modification of subcellular organelles of rhodotorula expansa.

The invention provides an ultra-high performance liquid chromatography-quadrupole rod / electrostatic field orbitrap high-resolution mass spectrometry detection method for a four-season antiviral mixture, which adopts ultra-high performance liquid chromatography-quadrupole rod / electrostatic field orbitrap high-resolution mass spectrometry, and the optimal chromatographic conditions are as follows: 0-1.0 min, 98%-98% A and 2.0%-2.0% B; 1.0-14.0 min, 98%-70% of A and 2.0%-30.0% of B are added; in 14.0-25.0 min, 70%-0% of A is adopted, and 30%-100% of B is adopted; 25.0-28.0 min, 0%-0% of A and 100%-100% of B are included; from 28.0 min to 28.1 min, 0%-98% of A is adopted, and 100%-2% of B is adopted; 28.1-30.0 min, 98%-98% of A and 2%-2% of B are included; 148 chemical components, including 31 glycosides, 14 terpenoids, 54 flavonoids, 10 lignans, 23 organic acids, 3 alkaloids and 13 other types, are identified in total. The detection method can provide reference for internal quality detection of the traditional Chinese medicine, research on basic components of pharmacodynamic substances of the traditional Chinese medicine and authenticity identification of traditional Chinese medicine components.

The present invention provides a tricyclic diterpenoid compound having anti-influenza virus activity. The tricyclic diterpenoid compound not only has better anti-H1N1 virus activity, but also is low in toxicity. The invention also discloses a preparation method of the tricyclic diterpenoid compound with antiviral activity. The preparation method comprises the following steps: (1) obtaining a fermentation product rich in the tricyclic diterpenoid compound through microbial fermentation culture; a recombinant vector adopted in the microbial fermentation culture carries nucleotide sequences: PQ793156 (dioxygenase gene), PQ793157 (FAD dependent monooxygenase gene), PQ793158 (terpenoid synthase gene), PQ793159 (UbiA family isopentenyl transferase gene), PQ793160 (GGPP synthase gene) and PQ793161 (amino transferase gene). And (2) separating and purifying the fermentation product obtained in the step (1) to obtain the tricyclic diterpenoid compound.

Described are liquid compositions that contain a desired (e.g., extracted) plant material such as cannabinoid, terpene, terpenoid, or the like, contained, e.g., dissolved, suspended, or emulsified, in the liquid, which contains surfactant; methods of preparing these types of liquid compositions; and methods of processing this type of liquid composition to collect, isolate, concentrate, or purify a desired target material contained in the liquid composition.