116 results about "Terpenoid" patented technology

The invention relates to the technical field of gene engineering, in particular to an atractylodes macrocephala terpene synthase gene AmTPS9 and a product coded by the same and application of the gene AmTPS9. The gene AmTPS9 has one of the following nucleotide sequences: (1) a nucleotide sequence as shown in SEQ ID NO.1; and (2) a nucleotide sequence which is obtained by substituting, deleting or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID NO.1 and expresses protein with the same function. According to the invention, a coding gene of an atractylodes macrocephala terpene synthase gene (AmTPS) is cloned from the rhizome of the atractylodes macrocephala, and the synthase can be applied to a pathway prepared by taking farnesyl pyrophosphate (FPP) as a substrate. By utilizing the gene provided by the invention, the content of terpenoids in bighead atractylodes rhizome can be increased through a genetic engineering technology.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a promoter of a pinus massoniana terpene synthase gene Pm TPS (-)-alpha-pinene and application of the promoter. According to the invention, a promoter fragment of a Pm TPS (-)-alpha-pinene gene and three homeopathic action elements with jasmonic acid response on the promoter fragment are obtained from pinus massoniana. A complete promoter fragment of the Pm TPS (-)-alpha-pinene gene of the pinus massoniana is transformed into arabidopsis thaliana by means of agrobacterium mediation, and the promoter can drive expression of a GUS gene in leaves of the arabidopsis thaliana or nicotiana benthamiana. GUS staining and GUS enzyme activity of transgenic Bensi tobacco leaves are reduced along with deletion of jasmonic acid response cis-acting elements on the promoter. Furthermore, the yield of pine resin terpenoids can be increased by utilizing the promoter and the homeopathic element thereof.

The invention relates to a recombinant genetically engineered bacterium for synthesizing terpenoids. The recombinant genetically engineered bacterium is saccharomyces cerevisiae in which a gal80 gene, an lpp1 gene and a dpp1 gene are knocked out and a heterologous MVA pathway gene and an AuoblA gene are integrated. According to the invention, a ophiobollin synthesis route is introduced into the saccharomyces cerevisiae in a heterologous manner for the first time, so that de novo synthesis of a ophiobollin skeleton compound, namely the ophiobollin F and an oxidation product of the ophiobollin F is realized; the synthesis of the ophiobolin F of 5.1 g / L is realized, which is also the highest sesterterpene heterologous synthesis yield reported at present; according to the invention, the ophiobolin 5-OH-21-CHO-opF with a new structure, which has excellent anti-cancer activity, is obtained through identification; the engineering strain of high-yield ophiobolin U is constructed, and the shake flask yield reaches 128.9 mg / L.

The present disclosure relates to methods of making cyclic terpenoids through substrate-controlled catalysis taking place in the presence of a supramolecular capsule.

This invention relates to the use of a natural terpene compound in pharmaceuticals, said natural terpene compound having the structure shown in formula (I), and having the function of preparing a compound for the prevention or treatment of hypoxia-inducible factor-1. α Use in medicines for diseases associated with abnormally elevated activity. This invention has discovered that the compound possesses hypoxia-inducible factor-1. α It exhibits inhibitory activity, effectively suppressing macrophage inflammatory responses and hepatocyte damage. It also demonstrates significant protective function against lipopolysaccharide / galactosamine-induced acute liver injury in mice, and can be used as a lead compound for the preparation of new drugs or drugs for the prevention and treatment of acute liver injury.

There is provided a recombinant cell comprising a plasmid expressing terpenoid synthase gene and one or more modification to the gene of the recombinant cell. Also provided is a recombinant cell for use in biosynthesis and a method of producing isoprenoid / terpenoid.

Described are liquid compositions that contain a desired (e.g., extracted) plant material such as cannabinoid, terpene, terpenoid, or the like, contained, e.g., dissolved, suspended, or emulsified, in the liquid, which contains surfactant; methods of preparing these types of liquid compositions; and methods of processing this type of liquid composition to collect, isolate, concentrate, or purify a desired target material contained in the liquid composition.

Provided herein are terpenoid- and cannabinoid-encapsulating PLGA nanoparticles and pharmaceutical compositions comprising the nanoparticles. Further provided are methods of making and using the terpenoid- and cannabinoid-encapsulating PLGA nanoparticles for therapeutic purposes.

The invention provides an engineering bacterium and a method for synthesizing dacryitol. Wherein the engineering bacteria comprise II type terpenoid synthetase and I type terpenoid synthetase, and the sequence of the II type terpenoid synthetase contains any one of SEQ ID NOs: 41, 32, 42, 14, 15, 33, 3, 40, 39, 23, 4, 5, 10, 13, 19, 20, 24, 26, 27, 35, 36, 37 or 87, or contains a sequence having more than 70% of homology with the amino acid sequence; the sequence of the type I terpene synthase contains a sequence shown as SEQ ID NO: 101, SEQ ID NO: 118, SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97 or SEQ ID NO: 88, or a sequence which has more than 70% of homology with the amino acid sequence. The method can solve the problem that high-efficiency biosynthesis of dacryitol is difficult in the prior art, and is suitable for the field of synthetic biology.

The invention relates to the technical field of gene engineering, in particular to an Atractylodes lancea terpene synthase gene AlTPS42, a product coded by the Atractylodes lancea terpene synthase gene AlTPS42 and application of the Atractylodes lancea terpene synthase gene AlTPS42, and the Atractylodes lancea terpene synthase gene AlTPS42 has a nucleotide sequence as shown in SEQ ID NO.1 or a nucleotide sequence which is obtained by substituting, deleting or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID NO.1 and expresses protein with the same function. The enzyme can be applied to a pathway prepared by taking farnesyl pyrophosphate (FPP) as a substrate. By utilizing the gene provided by the invention, the content of the terpenoid substances of the atractylodes lancea can be increased through a genetic engineering technology.

The invention belongs to the field of antioxidants, and particularly relates to a chitosan-based antioxidant as well as a preparation method and application thereof. The chitosan-based antioxidant provided by the invention is prepared by carrying out Michael addition reaction on an antioxidant with a structure as shown in a formula (I) and a biomass raw material and then carrying out amidation reaction with chitosan, the biomass raw material is one or more of itaconic acid, fumaric acid, mesaconic acid, muconic acid, aconitic acid, methacrylic acid, linoleic acid and terpenoids. According to the invention, a specific biomass raw material is taken as a bridging agent, and a small molecular antioxidant is grafted into chitosan through Michael addition reaction and amidation reaction, so that the molecular weight of the antioxidant is increased, and the thermal stability and migration resistance of the antioxidant are improved; meanwhile, a chitosan structure exists in the antioxidant, residual metal ions in the rubber emulsion can be chelated, the catalytic oxidation effect of the metal ions is inhibited, and the oxidation resistance of the antioxidant is improved.

The invention discloses a mushroom strain LV17 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.42460 and an application of the mushroom strain LV17. The strain can efficiently synthesize up to 64 key aroma substances in liquid fermentation, the yield of a core flavor substance dihydro-2-methyl-3 (2H)-furanone stably reaches milligram per liter, and the relative odor activity value is up to about 3.1 * 10 < 5 >; high-valence terpenoids such as beta, beta-ionone and the like are also remarkably enriched, so that strong and pure flower fragrance-fruit fragrance-sweet fragrance composite characteristics are jointly formed. Electronic nose and GC-MS (gas chromatography-mass spectrometry) analysis show that the overall aroma intensity of the strain is obviously superior to that of a control strain, and the response values of bad sulfides and amines are not abnormal. The strain can be applied to the fields of natural spices, food aroma enhancement and the like, and an efficient microbial cell factory is provided for solving the problem of production of natural characteristic aroma substances.

The invention discloses a pungent litse fruit essence for beverage and a preparation method thereof, and belongs to the technical field of beverage additives, the pungent litse fruit essence is prepared from the following raw materials in parts by mass: 0.1 part of citronellal, 0.1 part of linalool, 0.05 part of geraniol, 0.1 part of citral, 3 parts of lemon terpenoid removal liquid, 10 parts of pungent litse fruit water-soluble extract, 5 parts of pungent litse fruit fermentation extract, 61.65 parts of ethanol, 10 parts of purified water and 10 parts of propylene glycol. The citronellal, the linalool, the geraniol and the citral are used for providing head fragrance, the lemon terpene-removing liquid is used for providing soft fragrance, the pungent litse fruit water-soluble extract is used for providing main fragrance, the pungent litse fruit fermentation extract is used for providing composite fragrance, so that the fragrance of the pungent litse fruit essence is compounded and rich, and meanwhile, the pungent litse fruit extract is obtained by an ethanol water extraction method, has good water solubility, and can be used for preparing the pungent litse fruit essence. The beverage can be applied to beverages and has unique flavor.

This invention belongs to the field of natural product chemistry technology, and relates to the extraction of natural products from the Saxifragaceae family (…). Saxifragaceae Juss.) *Changshan* ( Dichroa Lour.) Plant of the Chinese tallow tree ( Dichroa febrifuga Nine terpenoid compounds were isolated from *Dichroa febrifuga* extract. These compounds were obtained by sequentially separating the crude extract using D101 macroporous resin, silica gel column chromatography, HP20 column chromatography, ODS column chromatography, and HPLC. This invention also relates to investigating the bioactivity of these terpenoid compounds. The preparation method of this invention is simple, reproducible, and yields compounds with high purity, exhibiting good anti-inflammatory activity.

The present document describes a capsule encapsulating a hydrophobic molecule for solubilization of said hydrophobic molecule in an aqueous media. The capsule comprises biological molecules comprising alkaloids, terpenoids, fatty acids and amino acids and peptides. These biological molecules form a hydrophobic core encapsulating the hydrophobic molecule and outer shell layer providing water compatibility. The capsules have a size of from about 100 nm to about 10 µm.

The invention discloses Irpex lacteus YYY-16, and the preservation number of the Irpex lacteus YYY-16 is GDMCC No: 67140. The invention further discloses a terpenoid and a preparation method thereof. The terpenoid has a structure shown in a formula I or a formula II. The invention further discloses application of the terpenoid in preparing tobacco pathogenic bacterium resisting drugs.

This invention belongs to the field of testing and analysis technology, and discloses a method for rapidly extracting and detecting the content of various terpenoids and sterols in rubber grass. The specific steps are as follows: Step 1: Preparation of standard solutions of terpenoids and sterols. Detection is performed using a general-purpose high-performance liquid chromatograph, eliminating the need for large-scale precision mass spectrometry equipment, resulting in lower purchase and maintenance costs. The operation process is simple and easy to learn, meeting the needs of high-throughput rapid detection of batch samples. A single sample test only takes 35-60 minutes, significantly improving detection efficiency. It can simultaneously complete the qualitative identification and content determination of five target components, including lupeol and taraxasterol. The method has good repeatability and high accuracy, with excellent linearity of the standard curve, and stable and reliable detection results. By optimizing the extraction solvent, temperature, time, and isocratic elution chromatographic conditions, the target components are fully extracted and separated effectively, allowing for accurate acquisition of the content data of each component.

The invention discloses a method for improving the solubility and stability of betulin, which is characterized in that oleanane terpenoid substance co-assembly and protein-polysaccharide composite matrix encapsulation are utilized to form a betulin loading system with a nanoscale core and a micron-scale shell, so that the solubility and stability of betulin are remarkably improved, and the controlled release of betulin in intestinal tracts is realized. The particle size of the compound formed by assembling the betulin and the oleanane terpenes is 100-120nm, and the compound is uniform in distribution, good in water solubility and high in thermal stability, pH stability and salt stability. After protein-polysaccharide loading is utilized, the water solubility and stability of the assembled compound are further improved, and the compound has the performance of controlled release of betulin in intestinal tracts, also has a synergistic anti-oxidation effect, and is beneficial to improving the bioavailability of the compound.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production through cofactors and product transport. According to the invention, an efficient stevioside biosynthesis system is constructed: in the aspect of chassis cell optimization, transcription factors for regulating and controlling an MEP pathway and key genes of the MEP pathway are overexpressed, and supply of terpene precursors is enhanced; the introduction of a heterologous MVA pathway achieves sufficient supply of terpenoid precursors. Meanwhile, on the cofactor regulation level, an NADPH regeneration network is constructed, so that the coenzyme level is improved, and the ATP synthesis module and the carbon metabolism flux are synchronously enhanced; in order to solve the problem of product transport, an efflux transport system is constructed, efflux of stevioside is improved, and pressure on cells is reduced, so that the production capacity of stevioside is remarkably improved.

The application belongs to the technical field of lithium batteries, and particularly relates to a full-solid-state composite positive electrode sheet and a preparation method and application thereof, and comprises the following steps: mixing a positive electrode active material, a solid-state electrolyte and a conductive agent to obtain a first mixture; mixing an additive terpenoid compound with the first mixture under high-temperature conditions to obtain a second mixture; mixing a binder with the second mixture under low-temperature conditions to obtain a third mixture; and fiberizing the third mixture to obtain the composite positive electrode sheet after molding. Compared with the prior art, the application solves the problems that the volume shrinkage and expansion of the positive electrode active material of the full-solid-state battery in the prior art deteriorate the interface contact, and the adhesive polytetrafluoroethylene in the dry process cannot provide sufficient interface adhesion. The scheme effectively enhances the interface contact, significantly reduces the loss of the positive electrode solid-solid contact, so that the full-solid-state battery realizes excellent long-cycle capacity retention.

Provided herein are topical pharmaceutical compositions of midostaurin or a pharmaceutically acceptable salt thereof and optionally a penetration enhancer (e.g., terpenoid such as menthol). Further described are methods for preparing such topical pharmaceutical compositions and methods of using the same for treating a subject.

A persistent encapsulation structure capable of preventing the degradation of bioactive substances, which encapsulation structure comprises a variety of encapsulation structure bodies (10), characterized by that the respective encapsulation structure body (10) has a polysaccharide structure (11) by which a plurality of bioactive substances (12) are encapsulated, wherein the bioactive substance (12) is selected from a group consisting of a bioactive fatty substance (121) and a bioactive non-fatty substance (122); that the polysaccharide structure (11) comprises at least one polysaccharide, wherein the at least one polysaccharide is first thoroughly mixed, then sheared at high pressure or homogenized at high speed and finally atomized dried, such that the polysaccharide structure (11) forms branched polysaccharides and linear polysaccharides which form a multitude of network structures; that the bioactive fatty substance (121) is selected from a group consisting of glycerophospholipids, sphingophospholipids, gangliosides, L-α- / glycerylphosphorylcholines, phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositol, phosphatidylserines, phosphatidic acids, phosphatidylglycerol, ceramides, glucocerebrosides, glycolipids, cholesterols and fatty acid substances; and that the bioactive nonfatty substance (122) is selected from a group of coenzymes, vitamins, carotenes, carotenoids, curcuminoids, curcumin, curcumin extract, polyphenols, saponins, glycosides, terpenoids, ergothioneine, minerals, acid substances, functional polysaccharides, carbohydrates, functional proteins, peptides, amino acids and their derivatives.

The invention discloses an antiproliferative synergistic antifungal drug composition based on a notopterygium incisum source, and relates to the technical field of drug preparation, the antiproliferative synergistic antifungal drug composition comprises a first active component and a second active component, the first active component is an anti-proliferative active component and is selected from at least one of the following two compounds: class A: giant column alkane sesquiterpenoids; the class B is a cycloartane type triterpenoid compound; the second active component is a synergistic antifungal active component and is composed of an active component A and an active component B; the pharmaceutical composition takes a specific active component from notopterygium incisum as a core, and can accurately induce apoptosis of target cells, efficiently inhibit proliferation of the target cells, remarkably enhance the antibacterial activity of the antifungal drug and greatly reduce the clinical dosage and toxic risk of the antifungal drug; the natural active components are excellent in biocompatibility and high in medication safety, and can synchronously realize dual effects of disease treatment and infection protection.

The invention discloses an oyster ecological reef cluster specially designed for a soft substrate intertidal zone mud flat and based on timber pile fixation, and belongs to the technical field of marine ecological restoration and coast engineering. The invention provides a suspension type reef construction scheme based on the friction pile principle aiming at the problem that an existing gravity type artificial reef is prone to sedimentation on a silty and muddy beach. The ecological reef cluster is composed of a plurality of reef body units, each unit comprises a pine pile set, and net bags are hung and fixed through rope knots. Trace terpenoids released by the pine material and biological membranes on the surfaces of the scallop shells have a synergistic effect, so that the attachment rate of the oyster larvae is remarkably increased. According to the method, through a'biological armor 'mechanism, the wood piles are wrapped and protected from being corroded by ship maggots through rapid calcification of oysters, a hydrodynamic field is optimized through 50-meter large-interval staggered layout, water body exchange and bait supply are guaranteed while wave energy is reduced, and the problem that structural stability and biocompatibility are difficult to consider in ecological restoration of the soft foundation beach is solved.

The application belongs to the technical field of biological medicine, and more particularly relates to application of a compound in preparation of a medicine for removing protein-bound uremic toxins in blood. The compound has a structural general formula shown in formula (I): wherein R1 is an aromatic heterocycle with a carbon number of 6-50, a benzenepropyl aromatic heterocycle with a carbon number of 6-50, or an aromatic hydrocarbon with a carbon number of 6-50; and R2 is a flavonoid group with a carbon number of 13-50, a polyphenol group, or a terpenoid group. Experiments show that the compound has a competitive replacement removal effect significantly better than that of the currently best replacement medicine, shikonin acid (LA), when the compound is used to remove protein-bound uremic toxins (PBUTs) such as IS and PCS by using a competitive replacement method, and the advantage is more obvious at a low concentration.

The invention discloses colletotrichum 7060 #, belongs to the technical field of microorganisms, and is used for solving the problems of low survival rate of aquilaria sinensis seedlings, long growth cycle and lack of growth-promoting microorganism resources. According to the technical scheme, the colletotrichum 7060 # with the preservation number of CGMCC NO.42267 and the biological product of the colletotrichum 7060 # and the method for promoting agilawood growth are provided, and the method is achieved through strain activation, cake making and symbiotic culture. Preferred schemes involve addition of a host root extract, staged supply of terpene signal molecules, use of a controlled release device with a buffer layer, strain pre-adaptation and addition of an energy slow release component. The agilawood growth can be effectively promoted, the biomass and stress resistance are improved, and reliable microbial resources and technical support are provided for agilawood seedling culture.

The present invention relates to a personal care composition comprising an unsaturated fatty acid selected from mono-unsaturated or poly-unsaturated fatty acids having carbon chain length from 14 to 24; and a terpenoid. Particularly, the present invention relates to said personal care composition that provides improved anti-aging benefit. Additionally, the present invention also relates to said personal care composition that improves the barrier function of the skin.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing abscisic acid and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and bioengineering. According to the invention, heterologous synthesis of ABA is realized in the Yarrowia lipolytica, precursor supply is optimized by enhancing carbon flow in a metabolic pathway, the activity of key enzymes BcABA2 and BcABA3 is improved by adopting a semi-rational strategy, NADPH regeneration engineering is implemented to enhance the oxygen uptake ability of a strain, a cytoplasm-peroxisome engineering system is constructed, and the oxygen uptake ability of the strain is enhanced. The ABA synthesis is further enhanced by utilizing the characteristic of high-yield acetyl coenzyme A of peroxisome. According to the invention, fermentation conditions are further optimized, so that the ABA accumulation concentration of the constructed engineering strain in a 5 L bioreactor reaches 2554.36 mg / L, and the highest titer of ABA biosynthesis reported so far is created. The metabolic modification strategy provides important reference for biosynthesis of other terpenoids.

This invention discloses a directed evolution strategy for SsDMS, a type II sesquiterpene cyclase derived from *Streptomyces showdoensis*, based on alanine scanning, and its applications. The invention describes several key mutant sites in SsDMS capable of catalyzing the generation of novel dried sesquiterpene compounds, including substitutions at positions 208, 248, 249, 497, and 505. This invention also discloses a highly efficient *E. coli* cell factory applied to the heterologous expression of SsDMS mutants and the efficient production of novel dried sesquiterpene compounds, further yielding structurally novel dried sesquiterpene compounds. This invention achieves the efficient production of dried sesquiterpene compounds using SsDMS and its mutants. Its modification strategy has broad applicability, providing important enzymatic resources for the biosynthesis of novel terpenoids and offering more options for the discovery of drug lead compounds.

The invention relates to the technical field of separation and purification of an underground part of ferula soongarica, in particular to a terpenoid component in a ferula soongarica extract as well as a preparation method and application of the terpenoid component, and the terpenoid component is obtained by the following method: adding the underground part of ferula soongarica into ethanol for soaking, heating, refluxing and extracting, and concentrating under reduced pressure to obtain a concentrated solution; sequentially extracting the concentrated solution with petroleum ether and dichloromethane, and concentrating to obtain petroleum ether part extract and dichloromethane part extract; and separating and purifying the dichloromethane part extract. An in-vitro anti-tumor experiment result shows that the obtained terpenoid component has a certain inhibition effect on HCG-27 (human gastric cancer cells) and can be applied to preparation of anti-tumor drugs and anti-tumor drugs.