321 results about "Terpenoid" patented technology

The invention discloses production of terpenoids and a strain, and belongs to the technical field of bioengineering. The serratia marcescens engineering bacteria for producing the semi-terpenoids or monoterpenoids are constructed, and can be used for producing linalool, isoprene, isopentenol, 1, 3, 4-triterpenes and 1, 3, 4-triterpenes. The composition is prepared from 1, 8-cineole, alpha-pinene, pinene, gamma-terpinene, geraniol, (+)-limonene, (-)-limonene, myrcene, beta-ocimene, sabinene, (-)-alpha-bisabolol, (-)-alpha-bisabolol, longifolene, wallenenes, beta-elemene, farnesene, patchouli alcohol, acyclic terpene and alpha-santalene. In a 30L fermentation tank, the yield of linalool produced by the serratia engineering bacteria is 40.72 g.L <-1 >.

The invention discloses a phoebe zhennan PzGGPS12 gene and application of the phoebe zhennan PzGGPS12 gene to improvement of yield and drought tolerance of plant terpenoids, and belongs to the field of genetic engineering, a key enzyme gene PzGGPS12 for catalyzing biosynthesis of the terpenoids in phoebe zhennan wood is screened and analyzed, and a full-length CDS nucleic acid sequence of the PzGGPS12 is obtained. Transgenic experiments prove that overexpression of the gene improves the relative content of tobacco monoterpenes and triterpenes. Short-term drought experiments prove that overexpression of the gene can promote biosynthesis of sesquiterpenes, diterpenes and triterpenes so as to enhance drought tolerance of transgenic tobacco. Therefore, the gene can be introduced into a plant as a target gene, the yield of terpenoids in the plant is increased, and the drought tolerance of the plant is enhanced so as to improve the variety of the plant.

The invention belongs to the technical field of lithium batteries, and particularly relates to an all-solid-state composite positive pole piece and a preparation method and application thereof, and the preparation method comprises the following steps: mixing a positive active material, a solid electrolyte and a conductive agent to obtain a first mixture; under a high-temperature condition, mixing an additive terpenoid with the first mixture to obtain a second mixture; under a low-temperature condition, mixing a binder with the second mixture to obtain a third mixture; and fiberizing the third mixture, and forming to obtain the composite positive pole piece. Compared with the prior art, the invention solves the problems in the prior art that the positive active material of the all-solid-state battery generates volume shrinkage and expansion to deteriorate interface contact, resulting in reduction of battery performance; and the problem that the adhesive polytetrafluoroethylene in a dry process cannot provide enough interface bonding force is solved. According to the scheme, the interface contact is effectively enhanced, and the loss of solid-solid contact of the positive electrode is remarkably reduced, so that the all-solid-state battery realizes excellent long-cycle capacity retention ratio.

The invention belongs to but not limited to the technical field of gene engineering, and discloses a method for improving the biosynthesis of yeast terpenoids and application of the method, and the method for improving the biosynthesis of the yeast terpenoids based on m7G methyltransferase complex genes TRM8-TRM82 comprises the following steps: overexpressing a TRM8 gene and a TRM82 gene; in a saccharomyces cerevisiae engineering strain genome for producing terpenoids, a promoter of a yeast endogenous TRM8 gene is replaced by a constitutive promoter PGK1p, and a promoter of a yeast endogenous TRM82 gene is replaced by a constitutive promoter TEF1p, so that the production efficiency of the saccharomyces cerevisiae terpenoids can be improved. The invention constructs a new wine brewing yeast engineering bacterium for producing the terpenoid lupeol as a representative compound, and the yield of the lupeol can be effectively improved. The new metabolic engineering method for improving the biosynthesis of the yeast terpenoids based on the TRM8-TRM82 has a remarkable effect.

The invention discloses saccharomyces cerevisiae recombinant bacteria for efficiently producing terpenoids as well as a construction method and application of the saccharomyces cerevisiae recombinant bacteria. According to the construction method, saccharomyces cerevisiae rich in terpenoid precursor farnesyl pyrophosphate is taken as a starting strain, the GAL80 protein level in a galactose induction system is regulated and controlled by introducing an N-Degron strategy, and meanwhile, a glucose concentration response type promoter HXT1 is introduced to effectively regulate and control the expression of GAL80 from the transcriptional level; the saccharomyces cerevisiae recombinant strain for efficiently producing the terpenoids can be obtained. The strategy is applied to a saccharomyces cerevisiae engineering strain of beta-elemene and patchouli alcohol, and a remarkable effect is achieved. And in the high-density fermentation process of the saccharomyces cerevisiae, the yield of the beta-elemene is obviously increased and reaches 4818.6 mg / L. The invention provides a solid research basis for sustainable biosynthesis of terpenoids, and has a wide application prospect.

The invention discloses a saccharomyces cerevisiae recombinant strain based on a uracil metabolism gene URA3 as well as a construction method and application of the saccharomyces cerevisiae recombinant strain. The construction method comprises the following steps: taking saccharomyces cerevisiae rich in farnesyl pyrophosphate as an original strain, introducing a uracil metabolism gene URA3, synchronously overexpressing a ribosome biosynthetic gene UTP10 and an iron metabolism gene FIT3, and utilizing the uracil metabolism gene URA3 to express up-regulation correlation with the ribosome biosynthetic gene UTP10 and the iron metabolism gene FIT3, so as to obtain the farnesyl pyrophosphate-rich saccharomyces cerevisiae. The metabolic flux of the terpenoids is enhanced, and the saccharomyces cerevisiae recombinant strain for efficiently producing the terpenoids is constructed. According to the method, a metabolic network of yeast is systematically optimized, and combined regulation and control on precursor flux distribution, protein translation capability and metal ion steady state are realized, so that the microbial synthesis efficiency of sesquiterpenoids such as patchouli alcohol and beta-elemene is remarkably improved.

The invention relates to the technical field of gene engineering, in particular to a grape transcription factor VvNAC53, application of the grape transcription factor VvNAC53 and a method for regulating and controlling the content of terpenoids in grapes. The nucleotide sequence for coding the grape transcription factor VvNAC53 is as shown in SEQ ID NO. 1; the content of terpenoids in grapes can be regulated and controlled by adopting VvNAC53; the regulation and control method comprises the following steps: transferring a grape transcription factor VvNAC53 into grapes and performing overexpression to reduce the content of terpenoids in grape fruits so as to directionally cultivate grape germplasm with different aroma types.

To provide a segment for a flavor inhalation article that suppresses crystallization of fragrance, which damages the product appearance over time.SOLUTION: Provided is a segment for a flavor inhalation article, the segment having a base material to which a molten liquid of a terpenoid compound has been added.SELECTED DRAWING: None

The invention discloses a method for preparing high-density aviation fuel by catalyzing turpentine with Bronsted acid. The method comprises the following specific steps: step 1, regulating the strength of Bronsted acid to catalyze the isomerization reaction of terpenoids in turpentine, firstly generating cyclohexene and cyclohexadiene intermediates, and then performing Diels-Alder dimerization addition reaction on the intermediates generated by isomerization under the catalysis of the acid to generate a polycyclic high-density fuel precursor with the carbon number of 20; and 2, carrying out hydrogenation on C = C double bonds in the double-ring structure of the high-density aviation fuel precursor with the carbon number of 20 under the catalysis of a hydrogenation catalyst so as to generate the high-density aviation fuel. The invention provides a novel method for preparing high-density aviation fuel from biomass platform compound turpentine, and the whole preparation process is simple and convenient to operate.

A method for preparing nanoparticles of triterpenoid small molecules co-assembled with Ce6 and copper ions wrapped in cancer cell membranes and its application. The present invention prepares a pure natural co-assembled nanotransmission system with new morphological characteristics and multiple biological activities - terpenoid compound and photosensitizer co-assembled nanoparticles. In the composite nanoassembly, triterpenoid compounds and photosensitizer Ce6 are assembled into nanoparticles, providing effective and safe chemotherapy and photodynamic therapy. Subsequently, Cu is complexed on the surface of the nanoparticles. 2+ , when Cu is introduced 2+ When activated, the reduced PDT effect caused by excessive glutathione in the tumor consuming reactive oxygen species can be counteracted. Furthermore, it can enhance CDT therapy through a Fenton-like catalytic reaction with overexpressed H₂O₂ at the tumor site. Finally, these molecules are encapsulated by the tumor cell membrane. By constructing CM@OABACe6 / CuNPs with homologous targeting, a triple synergistic platform for cancer treatment using PDT, chemotherapy, and CDT has been created.

Described are liquid compositions that contain a desired (e.g., extracted) plant material such as cannabinoid, terpene, terpenoid, or the like, contained, e.g., dissolved, suspended, or emulsified, in the liquid, which contains surfactant; methods of preparing these types of liquid compositions; and methods of processing this type of liquid composition to collect, isolate, concentrate, or purify a desired target material contained in the liquid composition.

The present invention relates to (1) a method for producing biochar having bio stimulating effects, (2) biochar that has been produced to having increased bio stimulating effects, (3) a method for capturing material extracted from biochar and (4) biochar extracts, comprising desired compounds extracted from biochar and suspended in an extract solution. The methods above all involve obtaining a particular biomass source and pyrolyzing the biomass source under specific conditions to increase the bio stimulating effects of the biochar. The method for capturing the material extracted from the biochar produced to have such stimulating effects includes contacting the biochar with a treating liquid, separating the biochar from the biochar extract, collecting the biochar extract from which the biochar has been separated and resuspending the biochar extract in an modified aqueous solution for further use and / or processing separate from, or prior to, disposal. The biochar extract suspended in solution then includes desired compounds (a) at least 0.1 ppm of one or more of the following phenylpropanoid, phenylalanine, flavonoids, sterols, terpenes, terpenoids, tannins, coumarins, benzaldehydes and phenols; (b) at least 0.1 g / L of humics, fulvics or a combination of both humics and fulvics and / or (c) one or more of the following salicylic acid, jasmonic acid, abscisic acid, cytokinin, gibberellic acid and ethylene, by way of example.

The invention discloses a method for producing terpenoids, and belongs to the technical field of bioengineering. The serratia marcescens engineering bacteria for producing the semi-terpenoids or monoterpenoids are constructed, and can be used for producing isoprene, isopentenol, 1, 3, 4-triterpenes, 1, 3, 4-triterpenes and the like. The composition is prepared from 1, 8-cineole, alpha-pinene, pinene, gamma-terpinene, geraniol, (+)-limonene, (-)-limonene, myrcene, beta-ocimene, sabinene, (-)-alpha-bisabolol, (-)-alpha-bisabolol, longifolene, wallenenes, beta-elemene, farnesene, patchouli alcohol, acyclic terpene and alpha-santalene. In a 30L fermentation tank, the yield of linalool produced by the serratia engineering bacteria is 13.49 g.L <-1 >.

A method of plant extraction comprising: (a) providing a plurality of trichomes from a plant at a first temperature below about 35 °C; and either: (b1) applying a negative pressure to the trichomes to volatize at least one desired compound from the trichomes to form a gaseous plant extract; and (c1) condensing the gaseous plant extract at a second temperature below about 25 °C to form a liquid plant extract; or (b2) combining the trichomes with a fluid to form a mixture; (c2) inducing cavitation in the mixture to release at least one desired compound from the trichome into the fluid; and (d2) separating out the at least one desired compound from the mixture; wherein the at least one desired compound is a terpene, a terpenoid, a cannabinoid, or a flavonoid.

The invention discloses a composition for reversing skin cell aging and application thereof, and relates to the technical field of cosmetics. The invention provides a composition. The composition comprises a component A and a component B, the component A comprises a camellia nitidissima extract, a camellia yunnanensis flower extract and a catechu extract; and the component B is oat beta-glucan. When the camellia nitidissima extract, the camellia yunnanensis flower extract and the catechu extract cooperate with the oat beta-glucan, small-molecule terpenoids and the like in the extracts can be used as natural penetration enhancers to form molecular channels with the oat beta-glucan; meanwhile, the components are synergistic, so that the removal of senescent cells can be enhanced, the cell regeneration is further enhanced, the cutin is effectively removed, the skin is brightened, and the mild anti-aging and brightening effects on the sensitive skin are achieved.

The present invention is related to an alcoholic formulation comprising a mixture of alkaloids, specifically piperine, piperettine, trichostaquine, piperoleins, other alkamides, and / or other alkaloids, as well as terpenoids, phenolics, prenol lipids, and flavonoids. The formulation is particularly rich in piperines, which are found in greater proportion compared to the other compounds. This unique composition exhibits anti-tumor activity in vitro, reducing tumors generated by orthotopic transplantation of tumors in animal models. Furthermore, it modulates the immune response by reducing the frequency of intratumoral immunosuppressive cells and increasing the frequency of cytotoxic T lymphocytes, thereby promoting tumor destruction and controlling metastasis.

The invention belongs to the technical field of microbial fermentation, and relates to bacillus cereus capable of biologically converting limonene, the bacillus cereus is preserved in the China Center for Culture Collection, the classification name is Bacillus cereus GJ-02, the preservation number is CCTCC M 20251438, and the preservation date is June 23, 2025; according to the invention, the bacillus cereus strain capable of biologically converting limonene and having efficient conversion capability is separated and screened, a new strain resource is provided for microbial conversion of D-limonene, and a theoretical basis is provided for biosynthesis and metabolic engineering modification of terpenoids; therefore, the method has a wide application prospect in the field of microbial fermentation.

The invention belongs to the technical field of microbial fermentation, and relates to a nitrate reducing pseudomonas capable of biologically converting limonene, which is preserved in the China Center for Culture Collection, the classification name of which is Pseudomonas nitritiducens GJ-01, the preservation number of which is CCTCC M 20251439, and the preservation date of which is June 23, 2025, and the preservation number of which is CCTCC M 20251439. According to the invention, the nitrate reducing pseudomonas strain with efficient conversion capability and capable of biologically converting limonene is separated and screened, so that a new strain resource is provided for microbial conversion of D-limonene, and a theoretical basis is provided for biosynthesis and metabolic engineering modification of terpenoids; therefore, the method has a wide application prospect in the field of microbial fermentation.

The invention belongs to the technical field of medicines, and particularly relates to terpenoids separated from purslane as well as a preparation method and application of the terpenoids. The chemical structural formula of the terpenoid is as shown in formula 1), a total extract of portulaca oleracea is firstly extracted, organic solvents with different polarities are used for extracting the total extract of the portulaca oleracea to obtain a petroleum ether layer, an ethyl acetate layer and an n-butyl alcohol layer, the ethyl acetate layer is repeatedly eluted through a silica gel column, an ODS reversed-phase column and a gel column, and the terpenoid is obtained. According to the present invention, the terpenoid is obtained through the isocratic elution of the collected same fraction through the HPLC chromatographic column and separation, and the terpenoid is obtained for the first time and shows the significant anti-inflammatory activity in vitro, such that the new choice is provided for the development of the natural anti-inflammatory drug. # imgabs0 #

The invention provides a strain of saccharomyces cerevisiae and application of the saccharomyces cerevisiae. The preservation number of the Filobasidium magnum is CGMCC (China General Microbiological Culture Collection Center) No.34231, and the Filobasidium magnum secretes lipoxygenase, protease and amylase. Compared with a control group fermented by sterile water, the saccharomyces cerevisiae fermentation group has the advantages that when the saccharomyces cerevisiae fermentation group is applied to the enhanced fermentation of the tobacco leaves, the contents of carotenoid degradation products, cembrane degradation products, Maillard reaction products, chlorophyll degradation products, terpenoid substances and the like of the tobacco leaves can be remarkably increased, the potassium-chlorine ratio can be increased by 50.1%, and the yield of the tobacco leaves can be increased by 50.1%. The total content of aroma components of the tobacco leaves is increased by 37.7%. According to the invention, the aroma amount, aroma richness, maturity, sweetness and combustibility of cigar tobacco leaves can be obviously improved, irritation and offensive odor are reduced, the aroma characteristics of sweet aroma, costustoot, bean aroma and the like are highlighted, and the fermentation period is shortened to 15 days.

The invention provides a collection and detection method for electronic cigarette atomized released substances, and belongs to the technical field of electronic cigarette detection. The method comprises the following steps: step 1, adsorbing and collecting aerosol release substances generated after atomization of electronic cigarette atomized liquid by using a solid-phase extraction column, wherein the solid-phase extraction column is filled with an adsorbing material polyethylene divinylbenzene; step 2, eluting the solid-phase extraction column after adsorption in the step 1 to obtain an eluent as a to-be-detected sample solution; and 3, detecting the content of a sweetening agent in the to-be-detected sample solution obtained in the step 2 by using a liquid chromatograph, and detecting the content of a cooling agent and a flavor substance in the to-be-detected sample solution by using a gas chromatograph-mass spectrometer. The method provided by the invention has very good trapping efficiency on substances with various phase states and pH values in aerosol release substances of the electronic cigarette, especially has a relatively strong adsorption effect on low-carbon ester and terpene flavor substances, improves the detection accuracy, and is suitable for simultaneous detection of various components in electronic cigarette products.

The invention relates to the technical field of gene engineering, in particular to a kit and a method for increasing the content of terpenoids in grapes. According to the method, the kit is adopted, the grape transcription factor MYB1R1 is transferred into grapes and overexpressed, and the content of terpenoids in grape fruits is increased. According to the method disclosed by the invention, the MYB1R1 gene is over-expressed in grapes, so that grape plants with higher terpenoids content compared with wild types can be obtained, and the content of partial terpenoids in grape peels can be effectively increased, so that grape germplasm with rich flower fragrance can be cultivated; the method plays a positive role in improving the flower and fruit fragrance of varieties, cultivating new varieties and accelerating the breeding process.

The invention discloses a compound fermentation method of acid-resistant probiotics and application of the acid-resistant probiotics in blueberry juice, and particularly relates to compound fermentation of plant lactobacillus B4 and plant lactobacillus SS6 and application of the compound fermentation method. The two strains are preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation numbers of the strains are respectively CGMCC NO.30881 and CGMCC NO.30880. The invention also discloses a preparation method of the strain. The viable count of the lactobacillus plantarum still exceeds 6 log CFU / ml after the lactobacillus plantarum is fermented for 72 hours in blueberry juice (pH = 3.0), and is two orders of magnitude higher than that of a common commercial strain (lactobacillus plantarum LP39); and the two strains are subjected to compound fermentation, so that the total phenol content, DPPH free radical scavenging rate and terpenoid content of the blueberry juice can be increased at the same time, and the influence of fermentation on the color of the blueberry juice can be reduced.

The invention relates to the technical field of gene engineering, in particular to an atractylodes macrocephala terpene synthase gene AmTPS9 and a product coded by the same and application of the gene AmTPS9. The gene AmTPS9 has one of the following nucleotide sequences: (1) a nucleotide sequence as shown in SEQ ID NO.1; and (2) a nucleotide sequence which is obtained by substituting, deleting or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID NO.1 and expresses protein with the same function. According to the invention, a coding gene of an atractylodes macrocephala terpene synthase gene (AmTPS) is cloned from the rhizome of the atractylodes macrocephala, and the synthase can be applied to a pathway prepared by taking farnesyl pyrophosphate (FPP) as a substrate. By utilizing the gene provided by the invention, the content of terpenoids in bighead atractylodes rhizome can be increased through a genetic engineering technology.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a promoter of a pinus massoniana terpene synthase gene Pm TPS (-)-alpha-pinene and application of the promoter. According to the invention, a promoter fragment of a Pm TPS (-)-alpha-pinene gene and three homeopathic action elements with jasmonic acid response on the promoter fragment are obtained from pinus massoniana. A complete promoter fragment of the Pm TPS (-)-alpha-pinene gene of the pinus massoniana is transformed into arabidopsis thaliana by means of agrobacterium mediation, and the promoter can drive expression of a GUS gene in leaves of the arabidopsis thaliana or nicotiana benthamiana. GUS staining and GUS enzyme activity of transgenic Bensi tobacco leaves are reduced along with deletion of jasmonic acid response cis-acting elements on the promoter. Furthermore, the yield of pine resin terpenoids can be increased by utilizing the promoter and the homeopathic element thereof.

The invention discloses a ginkgo natural extract copolymerized antibacterial conductive gel as well as a preparation method and application thereof. The conductive gel is prepared from the following raw materials in parts by mass: 1 to 10 parts of ginkgo natural extract, 1 to 8 parts of imidazolyl alkenyl amic acid compound, 0.02 to 0.1 part of stabilizer, 0.01 to 0.2 part of emulsifier, 0.5 to 1 part of organic silicon cross-linking agent, 1 to 5 parts of modifier, 0.05 to 0.3 part of organic oxidant, 1 to 10 parts of natural polysaccharide, 15 to 25 parts of organic solvent and 10 to 30 parts of water. The gingko natural extract has an antibacterial effect, micro-current is conducted by the gingko natural extract through a macromolecular chain and aromatic continuous conjugated structures in flavonoids and olefinic terpenoids, the antibacterial and conductive gel which is low in toxicity and can be applied to organism tissue repair is prepared, and the gel serving as a flexible medium can conduct electric signals and also can be used for repairing tissues of organisms. The foreign matter reaction risk can be reduced through the antibacterial function, meanwhile, the toxicity of metal ion leakage is avoided, and the method has unique advantages in the micro-current related fields such as flexible electronic sensors and bioelectrical stimulation dressings.

The invention discloses an application of a solidago canadensis allelochemical in inhibition of heterosylvus rugosa, and belongs to the technical field of water pollution control. The solidago canadensis allelochemical comprises the following components: 30-45% of a flavonoid compound; 40-50% of an organic acid compound; 0.5 to 5 percent of terpenoids; and 10-25% of alkaloid. The solidago canadensis allelochemical can be used for treating and controlling the outbreak of the red-tide heterobending algae through allelopathy, has a remarkable inhibition effect on the growth of the red-tide heterobending algae, not only can be used for treating the red tide with the red-tide heterobending algae as a dominant species, but also can be used for turning the solidago canadensis into wealth, and has two purposes.

To provide novel therapeutic compositions for systemic administration (e.g., oral administration) and long-term release of terpenoids and cannabinoids, and methods of making and using the therapeutic compositions.SOLUTION: The present invention provides a terpenoid-encapsulating PLGA nanoparticle, comprising a PLGA nanoparticle composed of copolymer poly(lactic-co-glycolic acid) and a first terpenoid encapsulated in the PLGA nanoparticle. The nanoparticle further encapsulates a second compound composed of (i) a cannabinoid or (ii) a second terpenoid other than the first terpenoid.SELECTED DRAWING: None

The invention relates to a recombinant genetically engineered bacterium for synthesizing terpenoids. The recombinant genetically engineered bacterium is saccharomyces cerevisiae in which a gal80 gene, an lpp1 gene and a dpp1 gene are knocked out and a heterologous MVA pathway gene and an AuoblA gene are integrated. According to the invention, a ophiobollin synthesis route is introduced into the saccharomyces cerevisiae in a heterologous manner for the first time, so that de novo synthesis of a ophiobollin skeleton compound, namely the ophiobollin F and an oxidation product of the ophiobollin F is realized; the synthesis of the ophiobolin F of 5.1 g / L is realized, which is also the highest sesterterpene heterologous synthesis yield reported at present; according to the invention, the ophiobolin 5-OH-21-CHO-opF with a new structure, which has excellent anti-cancer activity, is obtained through identification; the engineering strain of high-yield ophiobolin U is constructed, and the shake flask yield reaches 128.9 mg / L.

The present invention discloses terpene compounds derived from polar fungi, their preparation methods and applications, and respectively provides a calamenane-type sesquiterpene compound eutyacorane and a pimarane-type diterpene compound libertellenone Z derived from polar fungi. The structures are shown in the following formulas I and II respectively: The experimental results show that the two compounds have a better inhibition rate on the release of NO in the LPS-induced inflammatory model of mouse macrophages RAW264.7, providing a new type of choice for anti-inflammatory drugs.