This invention belongs to the field of
molecular biology technology, specifically relating to a set of SNP marker combinations, probe sets, and their applications for marker-assisted selection of apple single
fruit weight. The SNP marker combination consists of 16
SNP markers, including SNP1 to SNP16; the
nucleotide sequences of SNP1 to SNP16 are shown in SEQ ID NO.1 to SEQ ID NO.16, respectively. This invention focuses on major variable sites controlling single
fruit weight in *
Malus* plants, and has developed 16 major
SNP markers linked to the apple single
fruit weight trait, suitable for marker-assisted selection of apple single fruit weight during the
seedling stage of breeding materials.