49 results about "Malus" patented technology

The invention discloses an anti-ageing skincare product. The anti-ageing skincare product comprises 0.001 to 3 weight percent of a plant stem cell active matter and 0.01 to 3 weight percent of Dragosine carnosine, wherein the plant stem cell active matter is at least one of a Phytocelltec<TM> Malus Domestica apple stem cell, a Phytocelltec <TM> Solar Vitis grape stem cell, a Phytocelltec <TM> Alp Rose rose leave stem cell, a Phytocelltec <TM> Argan nut stem cell and a Regenistem Rice Ap red rice stem cell. The preparation method of the anti-ageing skincare product comprises a step of adding the plant stem cell active matter and the Dragosine carnosine into a skincare product. The anti-ageing skincare product comprises the plant stem cell active matter and the Dragosine carnosine at the same time, and under the synergistic action of the two components, the anti-ageing skincare product can obviously improve skin quality, skin elasticity and proliferative capacity of skin, and the effect is obviously higher than that of a skincare product in which the plant stem cell active matter or the Dragosine carnosine is added singly.

A formulation and/or composition comprising an epidermal stem cells activator and a dermal stem cells activator that have the potential of improving skin appearance. Methods of using such formulation and/or composition to improve skin appearance and to activate constituents of the skin.

The invention discloses an extract of fructus malus toringoides and fructus malus tiansitoria and a preparation and detection method of 2-O-beta-D-glucopyranosyl-L-ascorbic acid from the fructus malustoringoides and fructus malus tiansitoria. Experimental results show that the 2-O-beta-D-glucopyranosyl-L-ascorbic acid is successfully separated from the fructus malus toringoides and fructus malustiansitoria, and is discovered in the fructus malus toringoides and fructus malus tiansitoria for the first time. Therefore, the 2-O-beta-D-glucopyranosyl-L-ascorbic acid can be used for the identification and quality control over the fructus malus toringoides and fructus malus tiansitoria, and a foundation is laid for the excavation of pharmacodynamic effects of the fructus malus toringoides andfructus malus tiansitoria.

The invention provides a method for constructing Saccharomyces cerevisiae for efficiently synthesizing alpha-amyrin, which belongs to the field of bioengineering. The method comprises the following steps: carrying out semi-rational design on alpha-amyrin synthase derived from Malus domestica, and carrying out saturated mutation and iterative mutation on key amino acid residue sites to obtain a series of mutants including an MdOSC1 mutant with the highest catalytic activity; then performing overexpression on key genes in an alpha-amyrin synthesis route, and meanwhile, performing overexpressionon diacylglycerol acyltransferase (DGA1) so that the storage capacity of alpha-amyrin in saccharomyces cerevisiae cells is improved. The yield of the saccharomyces cerevisiae engineering strain capable of producing alpha-amyrin at high yield constructed by the method reaches 213.79 mg/L, efficient production of alpha-amyrin in saccharomyces cerevisiae is realized, and the engineering strain has important significance in production.

A transgenic plant or tree of Malus sp. comprising tissue derived from cells transformed with a nucleic acid molecule encoding a gametophytic S-RNAse from Malus sp. is provided. The nucleic acid molecule encoding a gametophytic S-RNAase is in a sense or antisense orientation relative to a regulatory region operably linked thereto. The regulatory region controls expression of the nucleic acid molecule, and the transgenic plant or tree exhibits self-compatibility compared to a corresponding non-transformed plant or tree.

The invention discloses an EPSP (5-enolpyruvyl shikimate-3-phosphate) synthase multisite mutant from Malus domestica, and a coding gene and application of the mutant. The mutant has 8 mutation sites, the amino acid sequence of the mutant is as SEQ ID No. 1, and the base sequence of the coding gene of the mutant is as SEQ ID No. 2. Tests show that the EPSP synthase multisite mutant from Malus domestica and the coding gene thereof have high glyphosate resistance, high PEP (phosphoenolpyruvic acid) affinity is kept, and accordingly possibility for using the coding gene for cultivation of transgenic crops is provided.

The invention belongs to the field of biological engineering breeding disclosing a method of increasing the conversion efficiency of Malus plants mediated by Agrobacterium using ultrasonic. Chipping the Malus octa-edged begonia leaves into small pieces, immerging in 75mg.L-1As Agrobacterium suspension with OD600 0.6 for two minutes, treating with 100 w ultrasonic, transferring to regeneration medium for resistance screening, transforming Malus octa-edged begonia leaves to obtain resistant callus and resistant seedling with a conversion rate of 2.2%. Ultrasonic technology is first applicated in the genetic transformation of Malus plants greatly enhancing transgenic efficiency of Malus plants. The application of ultrasonic assisted Agrobacterium-mediated gene transduction for transducting rolC and transforming Malus rootstock octa-edged begonia receives a 2.2% conversion efficiency, which is consumedly higher than ordinary Agrobacterium mediated method with 0.1 ~ 0.8% conversion efficiency.

The invention discloses an anti-wrinkle firming composition. The composition mainly contains tens of active ingredients, including lactobacillus/ soybean milk fermentation product filtrates, hydrolyzed Viola Tricolor extracts, euglena gracilis polysaccharides, malus domestica fructus cell culture extracts and the like, and the active ingredients are compounded with hydrolyzed collagen, glycerol, propanediol and the like. The anti-wrinkle firming composition can repair damaged skins, nourish skins, supplement skins with moisture, consolidate a repairing effect and accelerate skins to more favorably absorb the composition. After the composition is compounded, a proportion of irritant substances in the technical scheme is extremely low so as to be safe and reliable.

The invention relates to three mutations of a farnesyl diphosphate synthase (FPPS) gene of Malus domestica Borkh. and an identification method thereof, which belong to the field of biotechnology and provide a simple, practicable and cost-saving molecular marker breeding preselection method for breeding a superficial scald-resistant Malus domestica Borkh. variety. The identification method comprises the following steps of: designing a primer by using the sequence of the FPPS gene of the Malus domestica Borkh. and performing polymerase chain reaction (PCR) amplification by using a genome deoxyribose nucleic acid (DNA) of the Malus domestica Borkh. to obtain a PCR amplification segment; and sequencing and analyzing the PCR amplification segment of the superficial scald-resistant Malus domestica Borkh. variety and a superficial scald-susceptible Malus domestica Borkh. variety to find out genetic mutations related to characters of a superficial scald of the Malus domestica Borkh. at three positions, namely -262bp, -434bp and -600bp at the upstream of a transcription start point of the FPPS gene. By detecting the mutations through a molecular marker or sequencing method, the Malus domestica Borkh. variety and the characters of the superficial scald of fruits of a filial generation can be identified, and the characters of the superficial scald of the fruits of the filial generation of the Malus domestica Borkh. can be selected at an early stage so as to save cost for managing and screening progeny plants.

The invention discloses an MdABCG gene promoter deletion fragment, and an application thereof in detection of dwarfing of apple plants. A method for detecting the rootstock dwarfing capacity of appleplants, provided by the invention, comprises the following steps: detecting whether the genome of a to-be-detected apple plant contains the MdABCG gene promoter deletion fragment or not, and judging that the rootstock dwarfing capacity of the to-be-detected apple plant without the MdABCG gene promoter deletion fragment in the genome is higher than that of the to-be-detected apple plant with the MdABCG gene promoter deletion fragment in the genome, wherein the MdABCG gene promoter deletion fragment is from the -64th site to the -25th site in the upstream direction of A in an apple plant genomeMdABCG gene initiation codon ATG. The MdABCG gene promoter fragment deletion phenomenon is positively related to dwarfing traits, dwarfing plants in offspring can be rapidly screened by utilizing deletion and existence of the fragment, and the MdABCG gene promoter deletion fragment has great significance in the field of dwarfing of plants.

The invention provides a malus pumila Rhodomyrtus tomentosa fruit vinegar beverage and a preparation method thereof. The malus pumila Rhodomyrtus tomentosa fruit vinegar beverage comprises the following components by weight: 5-15 parts of a Rhodomyrtus tomentosa fruit vinegar stock solution, 20-45 parts of malus pumila juice, 3-15 parts of a sweetener, and 8-15 parts of calcium lactate. The preparation method includes: firstly preparing the Rhodomyrtus tomentosa fruit vinegar stock solution, then mixing the components, conducting boiling and filtering, and finally carrying out homogenizing, sterilizing and packaging to obtain a finished product. The malus pumila Rhodomyrtus tomentosa fruit vinegar beverage provided by the invention not only has unique taste and rich nutrition, but also has the effects of promoting digestion, increasing appetite, maintaining beauty and keeping young, helping produce saliva and slake thirst, and eliminating fatigue.

The invention discloses a rose glacier spring rejuvenating facial mask formula which comprises spring, rose hydrosol and various moisturizing and repairing ingredients. The various moisturizing and repairing ingredients specifically include: a main active ingredients and common ingredients; the main active ingredient is MetaTrixAPL-3 (first-generation stem cell products: MALUS DOMESTICA fruit cellculture extract, FRAGARIA VESCA fruit extract, GLYCINE SOJA seed extract, a hydrolyzed collagen, water, butanediol and 1,2-hexanediol; and effects: moisturization, wrinkle resistance and repairing).Specific effects of the ingredients in the rose glacier spring rejuvenating facial mask formula are that: the MALUS DOMESTICA fruit cell culture extract, the FRAGARIA VESCA fruit extract, the GLYCINESOJA seed extract, the hydrolyzed collagen, the water, the butanediol and the 1,2-hexanediol have effects of moisturizing, resisting to wrinkles and repairing; small molecule sodium hyaluronate has effects of moisturizing and resisting to wrinkles; sodium polyglutamate has effects of moisturizing and improving skin elasticity; and schizophyllin has effects of resisting to allergy, promoting woundhealing, resisting to ageing and moisturizing.

The invention discloses a Malus xiaojinensis MxVHA-c protein and a coding gene thereof, and application thereof. The Malus xiaojinensis MxVHA-c protein provided by the present invention is a protein (a) or (b) as follows: (a) a protein composed of an amino acid sequence shown in a sequence 1 in the sequence table; and (b) a protein derived from (a), related to iron deficiency tolerance of plant, and obtained by replacement and / or deletion and / or addition of one or more amino acid residues on an amino acid residue sequence of the sequence 1 in the sequence table. The MxVHA-c provided by the invention has important regulatory effect on plant with iron deficiency stress, and provides a good theoretical basis for further study on iron deficiency tolerance of Malus xiaojinensis.

The invention discloses an application of an R1R6 genotype apple as a receptor material in preparation of a red pulp transgenic apple, provides application of an R1R6 genotype apple as a receptor material in preparation of a transgenic apple, provides an application of the apple (Malus sp.) BCF2 as an acceptor material in preparation of a transgenic apple, and also provides a method for preparing the transgenic apple, which comprises the following step: performing genetic transformation on the Malus sp. BCF2 serving as a receptor material to obtain the transgenic apple. The inventor of the invention finds that in an F2 generation obtained by taking the apples of "purplish red No.1" and "Gala" varieties parents, and it is found that a strain with a genotype of R1R6 is easier to obtain a tissue culture seedling and has higher transformation efficiency as a genetic transformation receptor, the transformation rate of a G2 strain being the highest. The method disclosed by the invention has an important application value for the creation of the transgenic red-pulp apples.