50 results about "Malus" patented technology

The invention discloses an anti-ageing skincare product. The anti-ageing skincare product comprises 0.001 to 3 weight percent of a plant stem cell active matter and 0.01 to 3 weight percent of Dragosine carnosine, wherein the plant stem cell active matter is at least one of a Phytocelltec<TM> Malus Domestica apple stem cell, a Phytocelltec <TM> Solar Vitis grape stem cell, a Phytocelltec <TM> Alp Rose rose leave stem cell, a Phytocelltec <TM> Argan nut stem cell and a Regenistem Rice Ap red rice stem cell. The preparation method of the anti-ageing skincare product comprises a step of adding the plant stem cell active matter and the Dragosine carnosine into a skincare product. The anti-ageing skincare product comprises the plant stem cell active matter and the Dragosine carnosine at the same time, and under the synergistic action of the two components, the anti-ageing skincare product can obviously improve skin quality, skin elasticity and proliferative capacity of skin, and the effect is obviously higher than that of a skincare product in which the plant stem cell active matter or the Dragosine carnosine is added singly.

The invention provides a method for separating and purifying phloretin. According to the method, enzymolysis or acid hydrolysis is not adopted, the phloretin is separated from rosaceae Malus Sect. Sorbomalus plant first, the purity of the prepared phloretin can reach over 98 percent, and the phloretin is high in recovery rate; and the method provides a new way for the separation and puridicaiton of the phloretin, and basis is established for research on the exclusive components of the Malus toringoides(Rehd.) Hughes. and the Malus tiansitoria(Batal.)Schneid. simultaneously.

The invention provides application of Malus rowan and apple group plants to the preparation of medicines or health care food for reducing blood sugar. The invention also provides an extract of the Malus rowan and the apple group plants for reducing blood sugar and a medical composition containing the extract. The inventor finds that the Malus plants and extract thereof have obvious medical efficacy of reducing the blood sugar through experiments, and controls the quality through finger-print. The pharmacodynamics experiments prove that the medical efficacy of the Malus plants and the extract thereof is obvious, safe and stable.

The invention discloses a preparation method of a composition with anti-glycation and anti-skin-aging effects in the technical field of skin care product composition preparation. The composition withthe anti-glycation and anti-skin-aging effects comprises a phase A and a phase B, wherein the phase A is prepared from 0.1 part of sclerotium rolfssii gum, 2 parts of 1,3butanediol, 1 part of glyceroland 87.75 parts of water, the phase B is prepared from 5 parts of malus domestica fruit cell culture extracts, 2 parts of glycosylglycerol, 5 parts of water, 5 parts of nicotinamide, 5 parts of glycerol, 5 parts of caprylyl glycol, 5 parts of xanthan gum and 5 parts of salvia miltiorrhiza extracts. the skin effectively recovers the young state; the glycated protein is eliminated; the formed crosslinking Maillard reaction collagen is broken; the skin color unevenness and yellow color phenomena can be reduced; the composition can be used for mask essence liquid, and can also be used for essenceliquid and emulsion cream.

The invention discloses saccharomycete for controlling postharvest penicilliosis of malus domestica and an application of the saccharomycete, and belongs to the technical field of biological control. The saccharomycete is identified as Sporidiobolus pararoseus with the preservation number being CGMCC No. 2.5351. During use, the Sphaerotheca macrophylla is activated, cultured by an NYDB medium for 20 hours and then centrifuged, a thallus is obtained and diluted with sterile water, and the saccharomycete is obtained. With adoption of a saccharomycete suspension and extended penicillium spores for treating the malus domestica, the malus domestica is stored at 20 DEG C for 6 days, and results show that the higher the saccharomycete concentration is, the lower the decay rate is; when the saccharomycete concentration is 1*10<9> cells / mL, the penicilliosis decay rate of the malus domestica is 10.3%, and the penicilliosis decay rate of the control is 100%. Therefore, the Sporidiobolus pararoseus 16 can replace chemical fungicides to control occurrence of the postharvest penicilliosis of the malus domestica, harm of the chemical fungicides to people is avoided, and remarkable economic and social benefits are achieved.

A formulation and / or composition comprising an epidermal stem cells activator and a dermal stem cells activator that have the potential of improving skin appearance. Methods of using such formulation and / or composition to improve skin appearance and to activate constituents of the skin.

The invention discloses an extract of fructus malus toringoides and fructus malus tiansitoria and a preparation and detection method of 2-O-beta-D-glucopyranosyl-L-ascorbic acid from the fructus malustoringoides and fructus malus tiansitoria. Experimental results show that the 2-O-beta-D-glucopyranosyl-L-ascorbic acid is successfully separated from the fructus malus toringoides and fructus malustiansitoria, and is discovered in the fructus malus toringoides and fructus malus tiansitoria for the first time. Therefore, the 2-O-beta-D-glucopyranosyl-L-ascorbic acid can be used for the identification and quality control over the fructus malus toringoides and fructus malus tiansitoria, and a foundation is laid for the excavation of pharmacodynamic effects of the fructus malus toringoides andfructus malus tiansitoria.

The invention discloses a processing method of Malus domeri. The method utilizes a sodium chloride solution to protect the color of Malus domeri, and employs a vacuum microwave technology to dry Malus domeri. Malus domeri slices processed by the method provided by the invention have uniform color, are sour, sweet and tasty, and have rich nutrition. The method provided by the invention has the advantages of high processing efficiency, low cost, small loss of Malus domeri slices' nutritional ingredients, and good color, etc., significantly improves the added value of Malus domeri product, and achieves good economic benefits. The method provided by the invention belongs to the technical field of food processing.

The invention provides a method for constructing Saccharomyces cerevisiae for efficiently synthesizing alpha-amyrin, which belongs to the field of bioengineering. The method comprises the following steps: carrying out semi-rational design on alpha-amyrin synthase derived from Malus domestica, and carrying out saturated mutation and iterative mutation on key amino acid residue sites to obtain a series of mutants including an MdOSC1 mutant with the highest catalytic activity; then performing overexpression on key genes in an alpha-amyrin synthesis route, and meanwhile, performing overexpressionon diacylglycerol acyltransferase (DGA1) so that the storage capacity of alpha-amyrin in saccharomyces cerevisiae cells is improved. The yield of the saccharomyces cerevisiae engineering strain capable of producing alpha-amyrin at high yield constructed by the method reaches 213.79 mg / L, efficient production of alpha-amyrin in saccharomyces cerevisiae is realized, and the engineering strain has important significance in production.

A transgenic plant or tree of Malus sp. comprising tissue derived from cells transformed with a nucleic acid molecule encoding a gametophytic S-RNAse from Malus sp. is provided. The nucleic acid molecule encoding a gametophytic S-RNAase is in a sense or antisense orientation relative to a regulatory region operably linked thereto. The regulatory region controls expression of the nucleic acid molecule, and the transgenic plant or tree exhibits self-compatibility compared to a corresponding non-transformed plant or tree.

The invention discloses malus doumevi fermented wine, a preparation method thereof, malus doumevi fruit residue tequila, and a preparation method of the malus doumevi fruit residue tequila, and further discloses malus doumevi fruit wine prepared by mixing and blending the malus doumevi fermented wine with the malus doumevi fruit residue tequila and white granulated sugar. The technology advantagesof a fermentation method and a distillation method are integrated, and the obtained base wine can be drunk separately, and can also be drunk after being blended. After blending is performed accordingto the formula, the obtained finished product of the wine is clear, transparent, rich in fruit fragrance, moderate in sour and sweet degrees, fresh in mouth feel, and easy to preserve. A theory basisis provided for further development and utilization of the malus doumevi wine, the use of the malus doumevi is extended, and the added value of the malus doumevi is increased.

The invention discloses a strain of yeast with high ethyl acetate production under low temperature conditions, which belongs to the technical field of bioengineering. The strain is Wickerhamomycesano malus, which was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on January 19, 2015, with the preservation number CGMCC NO.10370. The optimum temperature of bacterial strain producing ethyl acetate of the present invention is 22 ℃, and acid resistance is pH2.5~7.0; Salt tolerance is 0~15% (NaCl%); Ethanol resistance is 0~10% (v% ), the metabolites produced by fermentation are only ethyl acetate and a small amount of ethanol, and do not contain fusel oil. The bacterial strain of the present invention can be used as an important functional bacterium in traditional fermentation industries and food industries such as vinegar, soy sauce, tempeh, noodle sauce, brewed wine, distilled wine, and prepared wine, so as to reduce the fermentation temperature of traditional production, optimize the production process, and reduce production. Energy consumption and cost are reduced, while increasing the content of ethyl acetate in the product, improving the flavor of the product, improving the quality of the product, and having considerable economic benefits.

The invention discloses an EPSP (5-enolpyruvyl shikimate-3-phosphate) synthase multisite mutant from Malus domestica, and a coding gene and application of the mutant. The mutant has 8 mutation sites, the amino acid sequence of the mutant is as SEQ ID No. 1, and the base sequence of the coding gene of the mutant is as SEQ ID No. 2. Tests show that the EPSP synthase multisite mutant from Malus domestica and the coding gene thereof have high glyphosate resistance, high PEP (phosphoenolpyruvic acid) affinity is kept, and accordingly possibility for using the coding gene for cultivation of transgenic crops is provided.

The invention provides a malus plant whole genome InDel marker genotype database and application thereof to germplasm resource specificity identification, and belongs to the technical field of molecular biology. A construction method for the genotype database includes the following steps: performing genotyping on InDel markers of apple germplasm resource genome DNA samples by using a primer pair and multiple PCR; recording by taking genotype of InDel markers corresponding to each malus germplasm resource as a row; and summarizing all germplasm resource records to obtain the malus plant whole genome InDel marker genotype database. The database provided by the invention can simply, conveniently, efficiently and stably identify the specificity of malus germplasm, overcomes the limitation thatDUS test is easily influenced by the environment, and provides a powerful tool for apple new variety protection and germplasm resource management.

The invention belongs to the field of biological engineering breeding disclosing a method of increasing the conversion efficiency of Malus plants mediated by Agrobacterium using ultrasonic. Chipping the Malus octa-edged begonia leaves into small pieces, immerging in 75mg.L-1As Agrobacterium suspension with OD600 0.6 for two minutes, treating with 100 w ultrasonic, transferring to regeneration medium for resistance screening, transforming Malus octa-edged begonia leaves to obtain resistant callus and resistant seedling with a conversion rate of 2.2%. Ultrasonic technology is first applicated in the genetic transformation of Malus plants greatly enhancing transgenic efficiency of Malus plants. The application of ultrasonic assisted Agrobacterium-mediated gene transduction for transducting rolC and transforming Malus rootstock octa-edged begonia receives a 2.2% conversion efficiency, which is consumedly higher than ordinary Agrobacterium mediated method with 0.1 ~ 0.8% conversion efficiency.

The invention discloses an anti-wrinkle firming composition. The composition mainly contains tens of active ingredients, including lactobacillus / soybean milk fermentation product filtrates, hydrolyzed Viola Tricolor extracts, euglena gracilis polysaccharides, malus domestica fructus cell culture extracts and the like, and the active ingredients are compounded with hydrolyzed collagen, glycerol, propanediol and the like. The anti-wrinkle firming composition can repair damaged skins, nourish skins, supplement skins with moisture, consolidate a repairing effect and accelerate skins to more favorably absorb the composition. After the composition is compounded, a proportion of irritant substances in the technical scheme is extremely low so as to be safe and reliable.

The invention provides an extraction method of plum-leaf crab polyphenol. The extraction method comprises the following steps of: raw material pretreatment, freeze drying, smashing, primary extraction of feed liquid, secondary extraction of feed liquid, concentration and recovery, purification, concentration and drying. The extraction method has the advantages of convenience in operation, controllable conditions, quantitative and accurate extraction, high purity of extracted polyphenol and convenience in rapid extraction of the plum-leaf crab polyphenol, and is suitable for extracting the plum-leaf crab polyphenol. By adopting the preparation method, the problems of difficulty in quantification of plum-leaf crab polyphenol extraction, low purity and incapability of accurately controlling operation are solved at a certain degree.

The disclosure provides an InDel marker genotype database of whole genome of Malus and use in specific identification of germplasm resources thereof, belonging to the technical field of molecular biology. The constructing method of the genotype database comprises: genotyping InDel markers of genomic DNA samples of Malus germplasm resources by using primer pairs and multiplex PCR; recording one line data for each Malus germplasm resource according to the genotype of InDel marker; collecting all germplasm resources records to obtain an InDel marker genotype database of the whole genome of Malus. The whole genome InDel marker genotype database of Malus provided by the disclosure can identify the specificity of apple germplasm simply, efficiently and stably, thus overcoming the limitation that DUS test is easily influenced by environment, and providing a powerful tool for the protection of new apple varieties and the management of germplasm resources.

The invention provides a method for separating and purifying phlorizin in crabapple (malus hupenhensis) water extract. The method concretely comprises the following steps: concentrating the crabapplewater extract at 75-85 DEG C to obtain extractum; cooling the extractum to the room temperature, then transferring the cooled extractum into a refrigeration storage at 0-5 DEG C, standing and carryingout crystallization for 20-30h; evenly mixing the crystallized extractum with ice water, separating and filtering to remove mother liquor; after the mother liquor is filtered out, adding the ice water for washing, and spin-drying to obtain a product containing the phlorizin; dissolving the crude phlorizin in ethanol at the room temperature, filtering, concentrating under reduced pressure, and collecting paste; adding hot water, evenly mixing, then standing and cooling; after the temperature is lowered to the normal temperature, transferring the product into the refrigeration storage at 0-5 DEG C, standing, and crystallizing for 20-30h to obtain the pure phloridzin. The affordable, stable and reliable method for separating and purifying the phlorizin in the malus hupenhensis water extractis developed by using the common industrial equipment and solvent, and has the characteristics of being simple in process, safe and reliable, easy to implement, high in recovery rate, low in cost, andthe like.

The invention relates to three mutations of a farnesyl diphosphate synthase (FPPS) gene of Malus domestica Borkh. and an identification method thereof, which belong to the field of biotechnology and provide a simple, practicable and cost-saving molecular marker breeding preselection method for breeding a superficial scald-resistant Malus domestica Borkh. variety. The identification method comprises the following steps of: designing a primer by using the sequence of the FPPS gene of the Malus domestica Borkh. and performing polymerase chain reaction (PCR) amplification by using a genome deoxyribose nucleic acid (DNA) of the Malus domestica Borkh. to obtain a PCR amplification segment; and sequencing and analyzing the PCR amplification segment of the superficial scald-resistant Malus domestica Borkh. variety and a superficial scald-susceptible Malus domestica Borkh. variety to find out genetic mutations related to characters of a superficial scald of the Malus domestica Borkh. at three positions, namely -262bp, -434bp and -600bp at the upstream of a transcription start point of the FPPS gene. By detecting the mutations through a molecular marker or sequencing method, the Malus domestica Borkh. variety and the characters of the superficial scald of fruits of a filial generation can be identified, and the characters of the superficial scald of the fruits of the filial generation of the Malus domestica Borkh. can be selected at an early stage so as to save cost for managing and screening progeny plants.

The invention relates to a Ganoderma lucidum crabapple tea and a preparation method thereof. The Ganoderma lucidum crabapple tea is made of Ganoderma lucidum powdered Begonia tea in bag, Ganoderma lucidum spore powdered Begonia tea in bag or Ganoderma lucidum fermented Begonia tea in bag. The ganoderma lucidum crabapple tea is a health product, and the invention applies the crabapple tea to the preparation, treatment or prevention of liver cell damage in vitro, chronic alcoholic liver cell damage, breast cancer, and lowering blood fat and blood sugar. The preparation process of the invention is simple, the material is easy to obtain, and the application in health tea is considerable. The health tea has a better synergistic effect on CCL4-induced liver cell injury. At the same time, the health tea has obvious inhibitory effect on ALT in the serum of mice with chronic alcoholic liver injury, showing a better synergistic effect. Can be widely promoted.

The invention discloses an MdABCG gene promoter deletion fragment, and an application thereof in detection of dwarfing of apple plants. A method for detecting the rootstock dwarfing capacity of appleplants, provided by the invention, comprises the following steps: detecting whether the genome of a to-be-detected apple plant contains the MdABCG gene promoter deletion fragment or not, and judging that the rootstock dwarfing capacity of the to-be-detected apple plant without the MdABCG gene promoter deletion fragment in the genome is higher than that of the to-be-detected apple plant with the MdABCG gene promoter deletion fragment in the genome, wherein the MdABCG gene promoter deletion fragment is from the -64th site to the -25th site in the upstream direction of A in an apple plant genomeMdABCG gene initiation codon ATG. The MdABCG gene promoter fragment deletion phenomenon is positively related to dwarfing traits, dwarfing plants in offspring can be rapidly screened by utilizing deletion and existence of the fragment, and the MdABCG gene promoter deletion fragment has great significance in the field of dwarfing of plants.

The invention provides a malus pumila Rhodomyrtus tomentosa fruit vinegar beverage and a preparation method thereof. The malus pumila Rhodomyrtus tomentosa fruit vinegar beverage comprises the following components by weight: 5-15 parts of a Rhodomyrtus tomentosa fruit vinegar stock solution, 20-45 parts of malus pumila juice, 3-15 parts of a sweetener, and 8-15 parts of calcium lactate. The preparation method includes: firstly preparing the Rhodomyrtus tomentosa fruit vinegar stock solution, then mixing the components, conducting boiling and filtering, and finally carrying out homogenizing, sterilizing and packaging to obtain a finished product. The malus pumila Rhodomyrtus tomentosa fruit vinegar beverage provided by the invention not only has unique taste and rich nutrition, but also has the effects of promoting digestion, increasing appetite, maintaining beauty and keeping young, helping produce saliva and slake thirst, and eliminating fatigue.

A malus sieboldii composite tea bag and a preparing method thereof are disclosed. The tea bag includes, by weight, 1.7-2.0 parts of malus sieboldii, 0.2-0.5 part of brown tea, 0.1-0.4 part of hawthorn fruit, and 0.1-0.3 part of fiveleaf gynostemma. The malus sieboldii comprises theophylline, flavonoid glycosides, a plurality of flavones, and other effective compounds, and has healthcare effects of strengthening the heart, resisting bacteria, lowering blood pressure, degrading fat, invigorating the stomach and the spleen, and the like. The tea bag is convenient to carry, rapid to brew, standard in amount, clean, sanitary, and convenient in flavoring and drinking. By combining rich nutrition components and special medical functions of the malus sieboldii, convenience is brought to human life, a malus sieboldii deep-processing product is increased and advantages of local resources are exerted.

The invention discloses a rose glacier spring rejuvenating facial mask formula which comprises spring, rose hydrosol and various moisturizing and repairing ingredients. The various moisturizing and repairing ingredients specifically include: a main active ingredients and common ingredients; the main active ingredient is MetaTrixAPL-3 (first-generation stem cell products: MALUS DOMESTICA fruit cellculture extract, FRAGARIA VESCA fruit extract, GLYCINE SOJA seed extract, a hydrolyzed collagen, water, butanediol and 1,2-hexanediol; and effects: moisturization, wrinkle resistance and repairing).Specific effects of the ingredients in the rose glacier spring rejuvenating facial mask formula are that: the MALUS DOMESTICA fruit cell culture extract, the FRAGARIA VESCA fruit extract, the GLYCINESOJA seed extract, the hydrolyzed collagen, the water, the butanediol and the 1,2-hexanediol have effects of moisturizing, resisting to wrinkles and repairing; small molecule sodium hyaluronate has effects of moisturizing and resisting to wrinkles; sodium polyglutamate has effects of moisturizing and improving skin elasticity; and schizophyllin has effects of resisting to allergy, promoting woundhealing, resisting to ageing and moisturizing.

The invention belongs to the field of biological engineering breeding disclosing a method of increasing the conversion efficiency of Malus plants mediated by Agrobacterium using ultrasonic. Chipping the Malus octa-edged begonia leaves into small pieces, immerging in 75mg.L-1As Agrobacterium suspension with OD600 0.6 for two minutes, treating with 100 w ultrasonic, transferring to regeneration medium for resistance screening, transforming Malus octa-edged begonia leaves to obtain resistant callus and resistant seedling with a conversion rate of 2.2%. Ultrasonic technology is first applicated in the genetic transformation of Malus plants greatly enhancing transgenic efficiency of Malus plants. The application of ultrasonic assisted Agrobacterium-mediated gene transduction for transducting rolC and transforming Malus rootstock octa-edged begonia receives a 2.2% conversion efficiency, which is consumedly higher than ordinary Agrobacterium mediated method with 0.1 ~ 0.8% conversion efficiency.

The invention discloses an MdABCG gene promoter deletion fragment and its application in detecting the dwarfing of Malus plants. The invention provides a method for detecting the dwarfing ability of rootstocks of plants of the genus Malus, comprising: detecting whether the genome of plants of the genus Malus contains a missing fragment of the MdABCG gene promoter; The rootstock dwarfing ability of the genus plant is higher than that of the tested Malus plant containing the MdABCG gene promoter deletion fragment in the genome; the MdABCG gene promoter deletion fragment is the upstream of A in the MdABCG gene initiation codon ATG of the Malus plant genome Direction ‑64th to ‑25th. The absence of the MdABCG gene promoter fragment is positively correlated with dwarfing traits, and the absence and presence of the fragment can be used to quickly screen dwarf plants in offspring, which is of great significance in the field of plant dwarfism.

The invention discloses a Malus xiaojinensis MxVHA-c protein and a coding gene thereof, and application thereof. The Malus xiaojinensis MxVHA-c protein provided by the present invention is a protein (a) or (b) as follows: (a) a protein composed of an amino acid sequence shown in a sequence 1 in the sequence table; and (b) a protein derived from (a), related to iron deficiency tolerance of plant, and obtained by replacement and / or deletion and / or addition of one or more amino acid residues on an amino acid residue sequence of the sequence 1 in the sequence table. The MxVHA-c provided by the invention has important regulatory effect on plant with iron deficiency stress, and provides a good theoretical basis for further study on iron deficiency tolerance of Malus xiaojinensis.