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Extraction method of plum-leaf crab polyphenol

An extraction method, the technology of crabapple fruit, is applied in the field of extraction of polyphenols from crabapple fruit, and achieves the effects of easy and rapid extraction, accurate extraction and quantification, and controllable conditions

Inactive Publication Date: 2017-09-29
FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The method realizes the extraction of polyphenols from crabapple fruit with quality and quantity through freeze-drying ultrasonic extraction and solid-phase extraction purification steps, and solves the technical problem of polyphenol extraction from crabapple fruit

Method used

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  • Extraction method of plum-leaf crab polyphenol
  • Extraction method of plum-leaf crab polyphenol

Examples

Experimental program
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Effect test

Embodiment

[0040] Take the fruits of 3 parts of crabapple fruits of narrow-leaved crabapple, flat-fruited crabapple and octagonal crabapple as materials, see Table 1, wash and chop the crabapple fruits into fruit pieces whose length, width and height are no more than 10 mm, and put the crabapple fruits into pieces. Put it into a vacuum freeze dryer, vacuum freeze-dry the crabapple fruit particles under the conditions of ≤20Pa and temperature -45~-55°C, and crush it with a homogenizer for 20-40 seconds after freeze-drying; weigh 5.00g of the crushed crabapple fruit, Put it into the ultrasonic equipment, set three 65%-85% ethanol solutions with solid-liquid ratios of 1:5, 1:10 and 1:15; pressurize ultrasonic treatment, ultrasonic frequency is 99KHz, and ultrasonic power is 850W-1250W; The ultrasonic time is 20-40min, and the ultrasonic temperature is 35°C-55°C. After the ultrasonic time is reached, the feed liquid is taken out; the feed liquid is centrifuged at 3000r / min for 15-25min, and t...

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Abstract

The invention provides an extraction method of plum-leaf crab polyphenol. The extraction method comprises the following steps of: raw material pretreatment, freeze drying, smashing, primary extraction of feed liquid, secondary extraction of feed liquid, concentration and recovery, purification, concentration and drying. The extraction method has the advantages of convenience in operation, controllable conditions, quantitative and accurate extraction, high purity of extracted polyphenol and convenience in rapid extraction of the plum-leaf crab polyphenol, and is suitable for extracting the plum-leaf crab polyphenol. By adopting the preparation method, the problems of difficulty in quantification of plum-leaf crab polyphenol extraction, low purity and incapability of accurately controlling operation are solved at a certain degree.

Description

technical field [0001] The invention relates to a method for extracting polyphenols from apples, in particular to a method for extracting polyphenols from apples, which is a method of freeze-drying, ultrasonic extraction and solid-phase extraction purification. Background technique [0002] Crabapple fruit is the main component of local farm apple resources in my country. Because of its unique flavor, rich nutritional value, and rich polyphenol content, the content is twice as high as that of cultivated apples. It has the functions of preventing high blood pressure, anti-tumor, anti-aging, and anti-cardiovascular Disease, anti-mutation, anti-radiation, anti-allergic, anti-caries, enhance muscle strength. However, the added value of fresh crabapple products is low, and the shelf life is short, making it difficult to transport over long distances. Therefore, it is of great significance to develop my country's crabapple processing industry, carry out research and development of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73B01D11/02A61K131/00
CPCA61K36/73A61K2236/15A61K2236/17A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55B01D11/0265
Inventor 赵继荣王昆王大江高源刘立军李连文
Owner FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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