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Method for extracting and purifying phloretin from Malus toringoides(Rehd.) Hughes. and Malus tiansitoria(Batal.)Schneid.

A purification method, the technology of phloretin, applied in the field of medicine, to achieve the effect of high recovery rate

Active Publication Date: 2012-10-03
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no method for directly separating and purifying phloretin from plants of the genus Malus without enzymatic hydrolysis or acid hydrolysis.

Method used

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  • Method for extracting and purifying phloretin from Malus toringoides(Rehd.) Hughes. and Malus tiansitoria(Batal.)Schneid.
  • Method for extracting and purifying phloretin from Malus toringoides(Rehd.) Hughes. and Malus tiansitoria(Batal.)Schneid.
  • Method for extracting and purifying phloretin from Malus toringoides(Rehd.) Hughes. and Malus tiansitoria(Batal.)Schneid.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 The separation and purification method of phloretin of the present invention

[0022] (1) Take 8 kg of dried leaves of Begonia variegata, crush or crush them by hand, weigh about 1 kg each time, add 5 times of 95% ethanol for reflux extraction, extract twice, filter, collect the subsequent filtrate, and recover under reduced pressure to obtain Ethanol site extract 563g. Add water to dissolve the extract of the ethanol part, then add 2 times of ethyl acetate and extract repeatedly until the ethyl acetate solution is colorless, combine the ethyl acetate solution, recover under reduced pressure to obtain 240 g of the extract of the ethyl acetate part.

[0023] (2) Dissolve the ethyl acetate part with an appropriate amount of methanol, and at the same time add 200g of silica gel (200-300 mesh) to mix the sample. Take 3000g of silica gel for wet packing, add the sample after the silica gel column is uniformly precipitated, and use chloroform-methanol=50: (1-3) ...

Embodiment 2 Embodiment 1

[0026] The structure identification of embodiment 2 embodiment 1 gained product

[0027] The phloretin obtained in Example 1 is carried out for structural identification, and the data are as follows:

[0028] This product is a light pink powder (methanol), sprayed with aluminum trichloride color developer under TLC ultraviolet lamp to display bright blue. The reaction of magnesium hydrochloride powder was positive, and it was preliminarily judged to be flavonoids or flavonols. Molecular formula: C 15 h 14 o 5

[0029] 1H-NMR (600MHz, DMSO-d612.23 (2H, s, 2', 6'-OH), 10.33 (1H, s, 4'-OH), 9.12 (1H, s, 4-OH), 7.03 ( 2H,s,H-3',5'),6.68(2H,d,J=8.5Hz),5.82(2H,s,H-3',5'),3.23(2H,d,J=7.6Hz ),2.78(2H,d,J=7.6Hz),13C-NMR(151MHz,DMSO-d6)205.0(C=O),164.7(C-4'),164.4(C-2',C-6' ),155.0(C-4),132.5(C-1),128.9(C-2,C-6),114.7(C-3,C-5)103.9(C-1')94.4(C-3' ,C-5'), 45.9(C-α), 30.0(C-β) were identified as the compound Phloretin by comparison with literature data.

[0030]

Embodiment 3

[0031] The purity analysis of the phloretin prepared by the method of the present invention in embodiment 3

[0032] The purity of the phloretin prepared in Example 1 is detected, and the conditions are as follows:

[0033] Instruments: Agilent1200 high-performance liquid chromatography, DiamonsilTM C18HPLC column (250×4.6mm, 5μm), BP211D electronic analytical balance (1 / 100,000, Sartorius Germany Sartorius AG).

[0034] Mobile phase: 27% acetonitrile-water

[0035] Detection wavelength: 286nm

[0036] Temperature: 30 degrees Celsius

[0037] Injection volume: 20μl

[0038] Elution condition: isocratic elution

[0039] result:

[0040] As determined by high performance liquid chromatography, the purity of the phloretin prepared by the method of the present invention reaches 98.61%, and the recovery rate is 65.12%.

[0041] The present invention does not adopt enzymatic hydrolysis or acid hydrolysis, and for the first time, phloretin is isolated from the plants of the Ros...

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Abstract

The invention provides a method for separating and purifying phloretin. According to the method, enzymolysis or acid hydrolysis is not adopted, the phloretin is separated from rosaceae Malus Sect. Sorbomalus plant first, the purity of the prepared phloretin can reach over 98 percent, and the phloretin is high in recovery rate; and the method provides a new way for the separation and puridicaiton of the phloretin, and basis is established for research on the exclusive components of the Malus toringoides(Rehd.) Hughes. and the Malus tiansitoria(Batal.)Schneid. simultaneously.

Description

technical field [0001] The invention relates to a method for extracting and purifying phloretin, belonging to the field of medicines. Background technique [0002] Phloretin, chemical name: 3-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)-1-propanone, is a flavonoid compound, soluble in methanol, ethanol and acetone, almost Insoluble in water. Phloretin is mainly distributed in the peels and root barks of juicy fruits such as apples and pears, and in horse drunken wood. It was first used as a natural skin whitening agent, which has the functions of moisturizing, treating acne with exuberant secretion, inhibiting the activity of melanocytes, and reducing spots. Recently, some domestic scholars have confirmed that phloretin has good pharmacological activities, such as anti-inflammation, improving immunity, preventing osteoporosis, lowering blood lipids, anti-tumor, anti-cardiovascular sclerosis, inducing cancer cell apoptosis, and inhibiting gastric cancer cells. Therefore, p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C49/83C07C45/78
Inventor 李敏王道清彭成
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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