75 results about "Rosaceae" patented technology

The invention provides a method for separating and purifying phloretin. According to the method, enzymolysis or acid hydrolysis is not adopted, the phloretin is separated from rosaceae Malus Sect. Sorbomalus plant first, the purity of the prepared phloretin can reach over 98 percent, and the phloretin is high in recovery rate; and the method provides a new way for the separation and puridicaiton of the phloretin, and basis is established for research on the exclusive components of the Malus toringoides(Rehd.) Hughes. and the Malus tiansitoria(Batal.)Schneid. simultaneously.

The invention discloses an active extract, the active extract is obtained by using ethanol water solution with 5 to 95 percent as a solvent to carry out the extraction from rosaceae and fagaceae plants, the content of phlorizin in the active extract is 30 to 99.5 percent; the invention further discloses a preparation method of the active extract and a usage thereof in the preparation of drugs for treatment for diabetic nephropathy and hepatic edema.

The invention relates to the application of rosaceae Potentilla plant total triterpene extract during the process of preparing the medicine for remedying and preventing II type diabetes mellitus, Potentilla plant total triterpene extract has an obvious remedy and prevention activity, is the natural medicine for remedying and preventing the II type diabetes mellitus, and has no poisonous and side action for remedying and preventing the II type diabetes mellitus.

The invention discloses a labeling method of an apple pollen tube microfilament. The labeling method comprises the following steps: cultivating apple pollen, curing the apple pollen, performing enzyme treatment on the apple pollen, treating the apple pollen with a surfactant, performing dye labeling, and performing scanning and imaging. The labeling method has the beneficial effect that the labeling method of gymnosperm pollen tube microfilament is referred and improved on the basis of the labeling method of pear pollen tube microfilament, dyes enter cells easier through curing, enzymolysis of cell walls and the surfactant, the interference of cell outer wall autofluorescence due to insufficient enzymolysis as TRITC-Phalloidin is applied are avoided when FITC-Phalloidin labeling is applied, so that relatively complete apple pollen tube microfilament can be stored; moreover, the labeling method is efficient and quick, can be used for obtaining relatively clear and bright pictures, thus filling up the blank of the apple pollen tube microfilament labeling, providing reference for labeling microfilament in rosaceae and angiosperm pollen tube, and achieving a high application value.

The invention discloses a method for tissue culture and rapid propagation of rosaceae Rosa deciduous erect shrub Rosa rugosa Thunb. belonging to the technical field of horticultural plant seedling propagation. The method is used for carrying out isolated culture on edible Rosa rugosa Thunb. caulicles to build a tissue culture and rapid propagation system of Rosa rugosa Thunb. and comprises four stages, namely selection and sterilization, primary culture, multiplication culture and rooting culture of an explant. The method disclosed by the invention is simple and has the advantages of high reproduction rate, short culture period and high reproduction coefficient, has relatively strong practicability in the actual production application, solves the technical problems of low reproduction rate and long reproduction period of a Rosa rugosa Thunb. seedling, and is suitable for commercial production of Rosa rugosa Thunb. seedlings so as to meet the urgent demand of a market.

The invention discloses a method for extracting a rosaceous plant root protoplast. The method for extracting the rosaceous plant root protoplast is as follows: enzymolysis of a rosaceous plant root is performed at first by utilization of enzymolysis liquid I and then performed by utilization of enzymolysis liquid II, and the rosaceous plant root protoplast is obtained; the enzymolysis liquid I is solution containing 210 to 420U / ml cellulose and the enzymolysis liquid II is solution containing 80 to 320U / ml cellulose and 1 to 4U / ml pectase. Both the output and the vitality of protoplasts obtained by the method are higher than those of the one-step enzymolysis method, and the extraction time is obviously shorter than that of the one-step enzymolysis method and generally requires 2 to 3 hours to complete the process, thereby damage of the method on the protoplasts is relatively small, consequently the method has wide application prospect.

The invention relates to a chaenomelessinensis (thouin) koehne leaf green tea and a manufacturing method thereof. According to the invention, shoots of chaenomelessinensis (thouin) koehne leaves are picked, and are processed through washing, enzyme deactivating, rolling, tipping and baking, such that the green tea is obtained. According to the invention, for the first time, the fresh shoots of the rosaceae chaenomele plant chaenomelessinensis (thouin) koehne is adopted as a raw material, and the material is processed with a green tea processing technology, such that a novel plant beverage with a good taste and a healthcare function is obtained. With the method provided by the invention, the chaenomelessinensis (thouin) koehne leaf green tea is advantaged in good appearance, firmly combined fiber, green and smooth color, brilliant green liquid color, fresh and rich taste, and refreshing and elegant fragrance. The polyphenol content of the tea is 7-10%; the flavonoid content is 3-6%; the soluble sugar content is 7-9%; and the amino acid content is 1-5%. According to the invention, oleanolic acid can be detected.

The invention provides a top grafting and cultivation method of cerasus yedoensis using cerasus conradinae as a stock. According to the method, the cerasus conradinae which belongs to the cerasus of rosaceae like the cerasus yedoensis is adopted as the stock, so that the method has the advantages that the affinity is high, wound healing is fast, and scions grow fast; the scions are soaked in treating fluid, and the steps and parameters of top multiple-head grafting are optimized, so that the reproduction speed of the cerasus yedoensis is increased; through each scion top grafted in spring, 20-30 branches can be sprouted in the same year, the longest growing length of each branch during defoliation in autumn and winter can reach 1.5 m, each bud can sprout 20-30 branches in the first half of the next year, and wounds heal properly in the third year; a crown is full, and top grafted seedlings can come out a nursery for being used in an engineering project.

The invention discloses a sorbus alnifolia tissue culture and rapid propagation method. Sorbus alnifolia belongs to the rosaceae sorbus deciduous trees, is a precious timber and a tree species in east China and north China, is a fine tree species for landscaping of northern cities and has a high medicinal value. A sorbus alnifolia seed is used as an explant, an aseptic seedling is obtained and is subjected to induction culture, multiplication culture, rooting culture, acclimatization, transplanting and the like to obtain the sorbus alnifolia in-vitro regeneration plant, the sorbus alnifolia tissue culture and rapid propagation technology system is established and the method has important practical significance on the promotion of the sorbus alnifolia scale development.

The invention belongs to the field of biological phosphorus accumulating technology, and relates to a method for removing soluble phosphorus from eutrophic water by using transgenic terrestrial plants. According to the present invention, the transgenic terrestrial plants comprise tobacco in Solanaceae and strawberry in Rosaceae, and the imported exogenous gene is polyphosphate kinase gene (ppk); after the transgenic plants are induced by water roots, a foam plate sealed with a nylon net is adopted to fix the transgenic plants, which float on the water surface; in the high concentration soluble phosphorus (50 mg / ml)-containing water, the maximum phosphorus removal rates of the transgenic tobacco and the transgenic strawberry are respectively 57.4% and 41.38%; in the low concentration soluble phosphorus (100 mug / ml)-containing water, the maximum phosphorus removal rates of the transgenic tobacco and the transgenic strawberry are respectively 98.8% and 94.3%. The method of the present invention is applicable for efficient biological removal of the phosphorus, biological treatment of the phosphorus-rich wastewater, biological restoration of the eutrophic water, and biological purification of the eutrophic water.

The invention discloses an establishment method of an S.Davidianavar. undulata. isolated culture regeneration system. S.Davidianavar. undulata. is a rosaceous stranvaesia evergreen dwarf shrub. The petals of the S.Davidianavar. undulata. are white, while the fruits of the S.Davidianavar. undulata. are orange; in autumn, the leaves of the S.Davidianavar. undulata. are vermeil, and the red leaf period lasts for one month; the fruits do not fall in winter; therefore, the S.Davidianavar. undulata. becomes incomparable rare trees species in ornamental flower-viewing, leaf-viewing, and fruit and flower-viewing shrubs in China at present. At present, the seedling propagation of the S.Davidianavar. undulata. is mainly performed in such manners of seed propagation and cutting propagation, and such manners have the problems of long period, low efficiency, high cost and the like, and fail in meeting the requirements of large-scale and standard production; as a result, the establishment of the S.Davidianavar. undulata. isolated culture regeneration system is necessary. According to the establishement method, the seed of the S.Davidianavar. undulata. is taken as an explant, an S.Davidianavar. undulata. in-vitro regenerated plant is obtained by virtue of the processes such as sowing, multiplication culture, rooting culture, and acclimatization and transplanting, and the S.Davidianavar. undulata. isolated culture regeneration system is established.

The present invention provides a highly safe antibacterial composition showing antibacterial activity against both Gram-positive bacteria and Gram-negative bacteria, of which flavor is not impaired. The antibacterial composition of the present invention contains nisin, and one or two or more kinds of plant-derived ingredients selected from the group consisting of juice, extract, and distillate of a Rosaceae plant, and a mixture of these. It is preferred that the Rosaceae plant is a plant belonging to the genus Prunus , it is more preferred that the plant belonging to the genus Prunus is Prunus mume , and it is particularly preferred that the plant-derived ingredient is Prunus mume fruit juice. The antibacterial composition of the present invention is useful especially as a composition for oral cavity.

A new orally administerable DHEA production promoter is provided. A composition containing an extract of at least one selected from the group consisting of plants of Rosaceae Crataegus, Saururaceae Houttuynia, Vitaceae Vitis, and Compositae Anthemis is provided as a DHEA production promoter. The extract may be of one of the plants or extracts of two or more of them may be used in combination. A specific example is preferably a mixture of extracts of all four of the aforementioned plants. This DHEA production promoter also can be used as, for example, an anti-aging agent such as a skin improver as well as a pharmaceutical agent such as an immunostimulator, an antidiabetic agent, an anti-osteoporosis agent, an antiarteriosclerotic agent, an anti-obesity agent, a sleep-promoting agent, and a central nervous system depressant.

The invention discloses a fragment assembly and specific primer for identifying rosaceous plant species and an application of the fragment assembly. The fragment assembly provided by the invention consists of single-stranded DNA as shown in a sequence 1 to a sequence 16 in a sequence table. The specific primer provided by the invention can be used for developing a common reagent kit for identifying rosaceous plant species, and can promote an application of plant DNA bar codes to social work.

The invention is directed to an absorbent article comprising a condensed tannin. The condensed tannin is extracted from a plant family which is selected from the group consisting of Ebenacea, Mimosoideae, Apiaceae, Pinaceae, Rosaceae, Fagaceae, and mixtures thereof. Since the condensed tannin has more hydroxyl groups and larger molecular structure than general polyphenol organic compounds, it cantrap malodor molecules more effectively than the general polyphenol organic compounds. In addition, the absorbent article of the invention can also provide a body treatment that protects the wearer'sbody and provides body comfort.

The present invention provides a deodorizing composition containing as an active ingredient thereof a natural plant extract with high safety, which can be used for foods without any anxiety concerning safety in use and shows a high deodorizing effect even in a weakly acidic condition, and foods and drinks containing such a composition. The deodorizing composition comprises a plant of the genus Rubus of the family Rosaceae, laccase and an acid.

The invention provides application of polysaccharide of rubus suavissimus S.Lee in inhibiting blood coagulation, wherein the polysaccharide of rubus suavissimus S.Lee is a mixture of water-soluble polysaccharide, oxalate soluble pectin polysaccharide and alkali-soluble polysaccharide extracted from rubus suavissimus S.Lee which is a rosaceous rubus.

The invention discloses a SOD (Superoxide Dismutase) protein of Prunus pedunculatacv.Wulate and a coding gene cloning method and a sequence thereof. According to the SOD protein and the coding gene cloning method and the sequence thereof disclosed by the invention, the full-length of the SOD gene of the Prunus pedunculatacv.Wulate is cloned by the RACE method and the coding protein of the SOD gene is analyzed to discuss the effect of the gene in improving oxidation resistance, salt stress and the like of transgenic plants. According to the SOD gene of the Prunus pedunculatacv.Wulate as a potential stress resistance candidate gene, cloning and functional analysis of the full gene of the SOD gene as well as genetic transformation to plants have certain meaning for culturing high stress resistance plant varieties. The SOD gene of the Prunus pedunculatacv.Wulate obtained by the method disclosed by the invention exerts an important effect in researching the antioxidation mechanism of the Prunus pedunculatacv.Wulate and the stress resistance of rosaceous plants.

The invention discloses a rosaceae and prunusoidea cross-genus cross-breeding method, and belongs to the technical field of plant hybridization. The method comprises the following steps of (1) pollination liquid preparation, (2) pollen treatment and artificial pollination, (3) bagging breeding, (4) microsome cultivation, and (5) grafting management. According to the method, through application andreasonable management of the pollination liquid, compared with traditional natural hybridization, the success rate of pollination is greatly increased, a corresponding hybridized female parent is obtained, and compared with a somatic cell fusion breeding technology, the technical threshold and the operation difficulty are reduced, so that the method is more feasible in subsequent popularization and application.

The invention provides a constitutive expression promoter of strawberry vein banding virus (SVBV). The promoter is a total-length promoter sequence or deficiency promoter sequence of the strawberry vein banding virus. In the constitutive expression promoter disclosed by the invention, the separated SVBV promoter and the deficiency construction product thereof can stably drive the expression of a target gene with high intensity on a rosaceous plant, a better tool is provided for the genetic engineering improvement of the rosaceous plant, and a new improvement means is provided for the whole plant genetic engineering. Moreover, a huge technical support is provided for the strawberry transgenic engineering, and a beneficial technical guarantee is provided for enriching the virus basic theory and intensifying practical application.

The invention provides an application of water-soluble polysaccharide extracted from a rosaceae rubus plant, rubus suavissimus, in protection of liver and activation of acetaldehyde dehydrogenase.

The present invention provides an enhancer for enhancing the activity of oxidized protein hydrolase. The oxidized protein hydrolase activity enhancer according to the present invention contains an extract of at least one plant selected from the group consisting of Compositae Anthemis, Saururaceae Houttuynia, Rosaceae Crataegus, and Vitaceae Vitis.

The invention provides a Rosaceae gum mask and a preparation method thereof, which belongs to the technical filed of daily use cosmetic. The Rosaceae gum mask comprises the following components in parts by weight: 20-30 parts of Rosaceae gum; 0.1-3 parts of an Aloe vera extract; 0.2-0.4 parts of a Calendula officinalis flower extract; 0.05-0.2 parts of a Hamamelis virginiana leaf extract; 0.01-1 part of a thickener; 1-30 parts of a polyol humectant; 1-5 parts of an adjuvant; and 31-78 parts of water. The Rosaceae gum mask of the invention has remarkable anti-inflammatory acne, and whitening and moisturizing effects.

The present invention discloses a klebsiella sp. and use thereof in preventing and treating of fire blight of fruit trees, and belongs to the technical field of biological prevention and treatment ofplant diseases. A TN50 strain which has a strong competitive effect and a remarkable inhibition effect on growth of erwinia amylovor is screened from endophytic bacteria separated from healthy Korla pear branches, is identified as klebsiella sp. and preserved in China General Microbiological Culture Collection Center (CGMCC) on November 18, 2020, and has the preservation number of CGMCC No.21204.A klebsiella TN50 strain fermentation broth and active metabolites in the fermentation broth have good prevention and treatment effects on fire blight of kernel fruit trees such as rosaceae pears, apples, haws, etc., and the TN50 strain is a biocontrol potential strain with a high prevention effect, good environmental safety and development and application prospects.

The application provides a composite cutting medium and a Rosaceae plant cutting medium and a preparation method thereof, and belongs to the technical field of Rosaceae plant cultivation media. The preparation method of the Rosaceae plant cutting medium includes mixing smashed leaves, branches or fruit of peach trees with nitrogenous fertilizer to obtain a mixture, wherein the mass ratio of C of the peach trees to N of the nitrogenous fertilizer is (20-25) :1; controlling water content of the mixture to be 40-55%, mixing the mixture with a microorganism compost promoter, composting to ferment,turning, decomposing and drying after the compost temperature is equal to or higher than 55 DEG C, wherein the microorganism compost promoter accounts for 0.1-0.4% of the compost by weight. The Rosaceae plant cutting medium obtained by the preparation method can realize good cuttage of Rosaceae plants, and can incrase cuttage survival rate of Rosaceae plants.

The present invention relates to a device for determining or studying the state of stimulation of the natural defenses of plants or plant portions, which plants advantageously belong to the Rosaceae family. The corresponding device includes means for determining the expression level, in a sample of plants or plant portions, of at least one target gene in each of the following groups (a) to (i):(a) PR-1, PR-2, PR-4 PR-5, PR-8, PR-14, PR-15; (b) PAL, CHS, DFR, ANS, PPO; (c) HMGR, FPPS, Far; (d) CSL; (e) APOX, GST, POX; (f) CalS, Pect, CAD; (g EDS1, WRKY; (h) LOX2, JAR; and (i) ACCO, EIN3. Said device preferably consists of a kit which contains a determination means in the form of pairs of primers, for implementing a quantitative PCR technique.

The invention discloses a processing method and device for anti-hypoxic fungus substitutional tea in the field of health product processing. The method includes performing handle cutting, slicing andcleaning on edible fungi, and mixing rosaceae plants and leguminous plants to perform banburying and moistening to obtain substitutional tea; and the banburying and moistening technology adopts sugared honey and distilled water to mix according to a ratio of 1 : 3 to be a banburying substrate, and the banburying technology can be accomplished through drying treatment for 3-6 hours at 100-130 DEG C. The device includes a banburying and moistening device and a stepping motor; the banburying and moistening device includes a case body having a feeding port and a discharging port; the case body issuccessively provided with a material stir-frying plate, a material bearing plate and a discharging inclined channel along a material supplying direction; a four-bar linkage structure is connected between the material stir-frying plate and the stepping motor; and the motion stroke of the material stir-frying plate is in contact with the surface of the material bearing plate. Compared with substitutional tea sliced by utilizing primordial matters, the sugar-coatings generated by the banburying and moistening technology can improve the smell of the substitutional tea, so that additional values can be enhanced.

The invention discloses a parasitism grafting method of rosa liana and rosaceae fruit trees. The method comprises the steps that robust rosaceae fruit trees without diseases or pests are used as hostplants, and full rosa liana seeds without diseases or pests are selected as parasitoidism plant seeds; the adopted seeds are sterilized and then used and can also be soaked for germination accelerating; an electric drill is used for punching, the standard is achieved that a hole deeply reaches the lignin and the trees are not punched completely, the hole is kept invariant, the area of the internalhole is large, and saw dust is removed; then, a mixed medium is placed, and rose seeds are wrapped by the mixed medium; then, a strapping tape is used for strapping, the matrix is not exposed, and the position of the hole is pricked by a needle; after seed germination, proper fixing is conducted until parasitism plant seeds grow to the size equal to the tree hole of a host, the skin layers of theparasitism plants and the host are slightly scraped and strapped in time, and the two kinds of plants can grow together. According to the method, nursery stocks of flower and fruit trees in an unusual shape can be cultivated, the pleasure is added to the life of people, and the trees have high ornamental value.

The invention discloses a rosaceae fruit picking device. The device comprises a storage box which is used for collecting fruits, a picking device which comprises a telescopic rod, a fixing clamp and acutting clamp, wherein the telescopic rod is arranged on the top of the storage box, the top of the telescopic rod is provided with the fixing clamp, the cutting clamp is arranged perpendicular to the axis of the telescopic rod and is located below the fixing clamp, a rotary bar is fixed to the axis of the top of the telescopic rod, and the top of the rotary bar is of a spherical structure and can be clamped in a hood. By means of the device, fruits can be easily picked and rapid sorting can be achieved.