Klebsiella sp. and use thereof in preventing and treating of fire blight of fruit trees

A technology for Klebsiella and fire blight, which is applied to Klebsiella and its application field in the biological control of fruit tree pear fire blight, and achieves the effects of significant antagonism, good control effect and simple fermentation process

Active Publication Date: 2021-02-05
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Klebsiella sp. and use thereof in preventing and treating of fire blight of fruit trees
  • Klebsiella sp. and use thereof in preventing and treating of fire blight of fruit trees
  • Klebsiella sp. and use thereof in preventing and treating of fire blight of fruit trees

Examples

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Embodiment 1

[0022] Example 1 Isolation, Purification and Screening of Endophytic Antagonistic Bacteria TN50 Strain in Fragrant Pear

[0023] (1) Isolation of endophytic bacteria in fragrant pear trees: Rinse the collected branches, flowers, leaves and fruits of healthy fragrant pear trees under running water for 1-2 hours, then rinse them with sterile water, weigh the petals, branches (cut into small pieces of 0.5~1 cm) and leaves and fruits (cut into 4mm 2 1 g each, soaked in 75% alcohol for 1 min, then soaked in 1% sodium hypochlorite for 2-5 min, and rinsed with sterile water for 3-5 times. Take 0.1 mL of the last sterile water rinse solution and spread it on the NA medium, place it in a 28°C incubator and incubate for 2-3 days, observe whether there are colonies growing, and check whether the surface of the sample is completely sterilized. Take a thoroughly sterilized sample on the surface and put it into a sterilized mortar, add 10 mL of PBS buffer solution to each 1 g sample to gri...

Embodiment 2

[0035] Identification of embodiment 2 TN50 bacterial strain

[0036] Strain TN50 was streak-inoculated on NA medium, cultured at 28°C for 24 h, and its colony morphology was observed and described. At the same time, Gram staining was carried out to observe the size, shape, presence or absence, shape and location of spores of the tested strains. The physiology and biochemistry mainly refer to the "Common Bacteria System Identification Manual".

[0037] (1) Colony characteristics and morphological characteristics of bacteria

[0038] TN50 colony characteristics such as ( image 3 ), the colonies on the NA medium are round, viscous and shiny, with neat edges, opaque, light yellow, and the morphological characteristics of the bacteria are as ( Figure 4 ) shows rod-shaped bacteria, G - , no spores, no flagella.

[0039] (2) Identification of physiological and biochemical properties

[0040] Refer to the method in the "Common Bacteria System Identification Manual" for determin...

Embodiment 3

[0046] Example 3 Determination of the control effect of Klebsiella TN50 strain on amygdalus blight in isolated inflorescences of fragrant pears

[0047] Flower branches were collected at the early flowering stage of the fragrant pear orchard, and inserted into 0.05% NaCl solution for antisepsis and moisturizing. Use a hand-held pressure sprayer to inoculate pear inflorescences with TN50 fermentation broth, spray about 80 µL of the bacteria spray solution on each flower, inoculate 50 inflorescences, and repeat 3 times; at the same time, spray agricultural streptomycin (produced by North China Pharmaceutical Factory, active ingredient 72 %) 4000 times liquid control, and sterile water as a blank control, after 24 hours of culture in an artificial climate box at 25°C and 70% air humidity, the pathogenic bacteria liquid was sprayed, about 80 µL per flower. Place the inoculated inflorescences in an artificial climate box at 25°C and 70% air humidity for cultivation. After 3 days, 5...

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Abstract

The present invention discloses a klebsiella sp. and use thereof in preventing and treating of fire blight of fruit trees, and belongs to the technical field of biological prevention and treatment ofplant diseases. A TN50 strain which has a strong competitive effect and a remarkable inhibition effect on growth of erwinia amylovor is screened from endophytic bacteria separated from healthy Korla pear branches, is identified as klebsiella sp. and preserved in China General Microbiological Culture Collection Center (CGMCC) on November 18, 2020, and has the preservation number of CGMCC No.21204.A klebsiella TN50 strain fermentation broth and active metabolites in the fermentation broth have good prevention and treatment effects on fire blight of kernel fruit trees such as rosaceae pears, apples, haws, etc., and the TN50 strain is a biocontrol potential strain with a high prevention effect, good environmental safety and development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to a strain of Klebsiella and its application in the biological control of fruit tree amylovora blight. Background technique [0002] Amylovora blight is caused by Erwinia amylovora ( Erwinia Amylovor ) is the most devastating bacterial disease caused by infecting a variety of Rosaceae plants. It is a first-class crop disease and agricultural plant quarantine pest in my country. The disease has a wide range of hosts, harming more than 220 species of plants belonging to more than 40 genera of Rosaceae, among which pome fruit trees such as pears, apples, hawthorns, and crabapples are the most susceptible. Pathogens infect flowers, leaves, young shoots, young fruits, branches and trunks, causing flower organs to wither, leaves withered and not falling like a fire, young shoots withered into a "shepherd's whip", fruit rot, black and shrunk, and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P1/00C12R1/22
CPCA01N63/20C12R2001/22C12N1/205
Inventor 罗明韩剑郁帆贺旭盛强古丽孜热包慧芳黄伟朱小琼
Owner XINJIANG AGRI UNIV
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