235 results about "Streptomycin" patented technology

The invention provides an adipose-derived stem cell composition for wound repair as well as a preparation method and application thereof, and belongs to the technical field of stem cells. The method comprises the following steps: adding fetal calf serum, dexamethasone, penicillin, streptomycin, collagen, magnetic particles and a P substance into a DMEM culture medium to prepare a culture medium, performing low-frequency magnetic field culture and separation on adipose-derived stem cells to obtain a stem cell solution, performing embedding to obtain stem cell microspheres, freeze-drying supernate to obtain a paracrine culture substance, and performing freeze-drying on the paracrine culture substance to obtain the adipose-derived stem cells. The adipose-derived stem cell composition for wound repair is prepared by mixing the adipose-derived stem cell with stem cell microspheres, hydroxypropyl methyl cellulose, carboxymethyl chitosan, hyaluronic acid, glycerol and stem cell microspheres, has better effects of implanting into damaged tissues, releasing nutrient substances, promoting neovascularization, regulating oxidative stress and inducing anti-inflammatory response, is beneficial to wound repair, and has a good application prospect. The gel can be converted into a gel phase at the skin epidermis temperature of 35-37 DEG C, components are stabilized, and sustained and controlled release is realized.

The invention relates to a norhalal-enoxacin conjugate as well as a preparation method and application thereof. The norhalal-enoxacin conjugate comprises a norhalal-enoxacin coupling compound, or a stereoisomer, a prodrug, a crystal form, a pharmaceutically acceptable salt, a pharmaceutically acceptable ester or a pharmaceutically acceptable solvate selected from the compound. Compared with enoxacin, kanamycin, streptomycin, lincomycin and azithromycin, the norharmann-enoxacin conjugate disclosed by the invention has a smaller MIC (Minimum Inhibitory Concentration) value, which shows that the norharmann-enoxacin conjugate has a better anti-staphylococcus aureus effect.

The invention discloses a culture medium and application thereof, and a method for in-vitro separation culture and adipogenic differentiation of precursor adipocytes in goat muscle, and relates to the technical field of cell culture, the culture medium comprises the following components: 8-12 [mu] g / mL of insulin, 8-12% by volume of fetal calf serum, 95-105 U / mL of penicillin, 95-105 [mu] g / mL of streptomycin and the like. On the basis of the prior art, a proper amount of liquiritin and pilocarpine are mainly added into a culture medium, and synergistic cooperation of the liquiritin and pilocarpine is found, so that the adipogenic differentiation capacity of cells such as mesenchymal stem cells and precursor adipocytes is remarkably improved, and the application range is wide. On the basis, the invention also provides a complete set of perfect in-vitro isolated culture and adipogenic differentiation method of the goat intramuscular precursor adipocytes, and a method and a way are provided for a series of researches developed through the goat primary intramuscular precursor adipocytes in the future.

The invention provides a stem cell extract composition for promoting hair growth and a preparation method thereof, and belongs to the technical field of cosmetics. Fetal calf serum, dexamethasone, penicillin, streptomycin, interleukin-2 and magnetic composite particles are added into a DMEM culture medium to prepare the culture medium, the culture medium is used for in-vitro culture of stem cells, during culture, a stem cell solution is prepared by combining a low-frequency magnetic field with low-energy laser culture, exosomes are extracted from an extracting solution, the exosomes and the stem cells are mixed and embedded to prepare embedded microspheres, and the embedded microspheres are used for in-vitro culture of the stem cells. The stem cell extract composition for promoting hair growth is prepared by uniformly mixing the stem cell extract with extracts extracted from fresh ginger, ginseng and polygonum multiflorum, the storage stability of stem cells and exosomes is obviously improved, hair growth is promoted, hair follicle development is promoted, mouse hair is induced and maintained in a growth period, and the stem cell extract composition is free of hormone addition, safe and non-irritant, and has a good application prospect. Wide application prospects are realized.

The invention discloses a human small cell lung cancer brain metastasis organoid culture medium and a culture method. According to the culture medium, an advanced DMEM / F12 culture medium is used as a basic culture medium, and the following components are added: 2 to 5 [mu] M of GlutaMax, 8 to 10 [mu] M of HEPES, 1 * to 2 * of B27, 500 [mu] M of SB202190, 5 to 10 [mu] M of Y-27632, 0.5 [mu] M of A83-01, 0.5 to 2 [mu] M of N-Acetyl-L-cysteine, 5 mM of Nicotinamide, 25 to 100 ng / ml of recombinant human FGF7, 100 to 500 ng / ml of recombinant human FGF10, 100 to 200 ng / ml of Human Noggin, 50 to 500 ng / ml of Human R-Spondin-1, 50 to 100 ng / ml of According to the culture method, TryPLE Express is used for dissociation, the time is short, the activity of dissociated single cells is high, and the success rate of organoid culture can be increased to the maximum extent. Meanwhile, by combining with a culture medium with an optimized formula proportion, formation and growth of brain metastatic organs of the human small cell lung cancer can be promoted, and the construction success rate is increased.

The invention provides a low-temperature-resistant inactivated guanidine salt virus preservation solution and a preparation method thereof, and relates to the field of virus preservation solutions. Comprising the following raw materials: NaCl, KCl, glucose, KH2PO4, Na2HPO4, guanidine hydrochloride, penicillin, streptomycin, amphotericin B and glycerol. The raw materials are mixed and uniformly stirred. The preserving fluid is suitable for being used in a low-temperature environment, can preserve a liquid state within several hours in the low-temperature environment, can significantly inactivate pathogens, can also maintain completeness of subsequent nucleic acid extraction, and has no significant influence on amplification and the like.

The present invention discloses a buffer for tissue separation and / or preservation and its application, and a method for culturing tumor organoids, and relates to the field of organoid culture. The buffer is used to preserve tumor tissue samples, which are of liquid origin; the buffer includes: a basal culture medium and specific additive factors, and the specific additive factors include: superoxide dismutase, catalase, penicillin, and streptomycin; based on the volume of the basal culture medium, the concentration of superoxide dismutase is 1 to 100 μg / mL, the concentration of catalase is 50 to 500 μg / mL, the concentration of penicillin is 50 to 200 U / mL, and the concentration of streptomycin is 50 to 200 μg / mL. The tissue preservation solution of the present invention is used for the transportation and protection of liquid-derived tumor tissue samples, and has a high tumor cell activity retention rate. It is used to construct tumor organoids with a high success rate and a large number, which can meet the needs of clinical detection and disease research.

The invention discloses a zebra fish spermatogonial stem cell separation and transplantation method, and belongs to the technical field of fish germline stem cell separation. The method comprises the following steps: washing complete sexual glands of zebra fish testis by adopting penicillin / streptomycin double-antibody-containing PBS (Phosphate Buffer Solution), physically shearing, digesting and dissociating, and filtering by a screen, so as to obtain zebra fish testis cell suspension; performing density gradient centrifugation to obtain zebra fish spermatogonial stem cells; dyeing, marking, centrifuging, washing, re-suspending, and carrying out micro-injection transplantation to enter the back of the abdominal cavity of the acceptor zebrafish juvenile fish. According to different physical properties of different cells, spermatogonial stem cells are separated, purified and transplanted from zebra fish testis cell suspension by adopting a density gradient centrifugation method, and migration and proliferation processes of donor germ cells can be observed in receptor fish after fluorescence labeling. And a new technical path is provided for fish germplasm resource preservation, endangered fish protection, economic cultured fish yield increase and new variety cultivation.

The invention relates to the technical field of stem cell induced differentiation, and discloses an umbilical cord mesenchymal stem cell adipogenic induced differentiation culture medium and a culture method. The culture medium comprises the following substances: a DMEM (Dulbecco's Modified Eagle Medium) / F12 basal culture medium, fetal calf serum, insulin, penicillin, streptomycin, dexamethasone, 3-isobutyl-1-methylxanthine, indometacin, BMP-2 and FGF-1 (Fibroblast Growth Factor 1). The culture method comprises the following steps: (1) carrying out primary isolation culture on umbilical cord mesenchymal stem cells; (2) carrying out passage on the umbilical cord mesenchymal stem cells; and (3) inducing adipogenesis of the umbilical cord mesenchymal stem cells by using the adipogenesis induced differentiation culture medium. BMP-2 and FGF-1 are added on the basis of a traditional adipogenic induction culture medium to induce and promote the umbilical cord mesenchymal stem cells to be more efficiently differentiated into adipocytes under the combined action, so that the adipogenic differentiation time is shortened, and the adipogenic differentiation efficiency is improved; the umbilical cord mesenchymal stem cell adipogenic culture medium is stable in formula, simple and convenient in culture method and suitable for wide popularization.

The invention provides a skin care composition containing stem cell extract as well as a preparation method and application of the skin care composition, and belongs to the technical field of cosmetics. The method comprises the following steps: mixing and culturing the extracted urine-derived stem cells and pilose antler stem cells in a DMEM culture medium added with epidermal growth factors, cholera toxin, penicillin, streptomycin, transferrin, IL-2 and IL-15, then separating a culture solution to obtain mixed exosomes, mixed stem cells and extracellular matrixes, preparing the mixed stem cells into stem cell lipidosome, and preparing the stem cell lipidosome. Embedding the stem cell extract and the mixed exosome into titanium dioxide / silicon dioxide microspheres, adding into an extracellular matrix, and uniformly dispersing to obtain the skin care composition containing the stem cell extract. The skin care composition containing the stem cell extract prepared by the invention can obviously prolong the preservation period of stem cells and exosomes and improve the repair and improvement of the stem cells and the exosomes on skin, and the prepared composition has better effects of resisting aging, whitening, promoting skin elasticity, resisting wrinkles and the like, and has a wide application prospect.

The invention belongs to the technical field of cell biology, and particularly discloses a culture medium and a culture method of fish primary liver cells. The culture medium of the fish primary liver cells comprises the following components: 90% of an M199 culture medium, 10% of fetal calf serum, 100 U / mL of penicillin, 0.1 mg / mL of streptomycin, 5 ng / mL of EGF, 10 [mu] U / ml of insulin and 10 mM of dexamethasone. Based on the culture medium, an efficient and stable fish liver cell culture method is developed by combining a standardized anesthesia and disinfection process, a precise dissection and liver sampling method, an optimized tissue digestion and cell separation scheme, an efficient cell purification and adherent culture system, a stable passage technology and the like; a standardized hepatocyte line establishment operation process is defined, and the problems that species binding exists in an existing fish liver cell culture method, and a standardized culture process suitable for multiple fish fingerlings is difficult to form are solved.

The invention belongs to the technical field of biology, and particularly relates to bifidobacterium longum subsp.infantis FMBL B250020GXY for metabolizing breast milk oligosaccharide and application of the bifidobacterium longum subsp.infantis FMBL B250020GXY, the bifidobacterium longum subsp.infantis FMBL B250020GXY is preserved in China Center for Type Culture Collection on September 1, 2025, and the preservation number is CCTCC M 20251931; three kinds of breast milk oligosaccharides, namely 2 '-fucosyllactose (2'-FL), lactose-N-neotetraose (LNnT) and lactose-N-tetraose (LNT), can be metabolized; plant source carbohydrates such as fructo-oligosaccharide and synanthrin can be metabolized; the compound has a relatively strong capability of removing DPPH and ABTS free radicals; a good inhibition effect is achieved on common pathogenic bacteria; good tolerance to acid and cholate is achieved; the strain is sensitive to gentamicin, streptomycin, tetracycline, chloramphenicol and vancomycin; the compound can be used for preparing antioxidant products, medicines for inhibiting pathogenic bacteria, infant foods and fermented foods, and has a wide application prospect.

The present invention discloses Streptomyces SC-3 and its composition and use. The Streptomyces SC-3 strain is deposited in China Center for Type Culture Collection with the deposit number CCTCC NO:M 2024906. The present invention finds that the Streptomyces SC-3 strain itself has high efficiency and broad-spectrum antibacterial activity, and finds that the Streptomyces SC-3 strain has a significant antagonistic effect on kiwifruit soft rot bacteria. It is verified by indoor experiments that the speed of kiwifruit fruit decay caused by Phomopsis can be slowed down. Its fermentation liquid has good temperature stability, and a variety of antibacterial substances are identified from its sterile fermentation liquid, including antibiotics such as antimycin, tetramycin, candidacidin, streptomycin, etc., which are widely used in biological control. The speed of fruit decay caused by the infection of Botrytis cinerea after inoculation can be effectively inhibited.

The invention relates to the technical field of preparation of carbon dioxide adsorbents, and discloses a carbon dioxide solid adsorbent and a preparation method and application thereof, the carbon dioxide solid adsorbent comprises 10-15 parts of aminated montmorillonite, 5-8 parts of modified chitosan, 20-25 parts of desulfurized gypsum, 3-5 parts of a composite water-retaining agent, 1-2 parts of a functional buffer agent and 0.5-1 part of an ammonia adsorption accelerant. According to the invention, the aminated montmorillonite is adopted to replace traditional streptomycin, so that the raw material cost is reduced, and the risk of antibiotic residue is thoroughly eliminated; chitosan is modified through graphene quantum dots, the excellent oxidation resistance of the graphene quantum dots is utilized, an antioxidant does not need to be additionally added, the environment burden is reduced while the preparation process is simplified, and the degradation rate of the adsorbent in the natural environment is increased. All the components in the adsorbent have a synergistic effect, the aminated montmorillonite provides abundant adsorption active sites, the modified chitosan enhances the mechanical strength and stability of the adsorbent, the composite water-retaining agent and the buffer agent guarantee the stability of an adsorption environment, and the ammonia adsorption accelerant accelerates CO2 adsorption kinetics.

The invention discloses a method for separating and transplanting spermatogonial stem cells of Chinese sturgeons, and belongs to the technical field of separation of germline stem cells of fishes. The separation method comprises the following steps: washing Chinese sturgeon testis tissues with an L-15 culture medium containing penicillin / streptomycin double antibodies, and carrying out physical shearing, digestive enzyme enzymolysis and screen filtration to obtain a Chinese sturgeon testis cell suspension; performing percoll density gradient centrifugation to obtain high-purity spermatogonial stem cells of the Chinese sturgeons; and dyeing, washing, resuspending and carrying out micro-injection transplantation to enter the gonad primordium reproductive ridge part of the juvenile acceptor fish. According to the physical characteristics of the spermatogonial stem cells of the Chinese sturgeons, the spermatogonial stem cells with high purity are obtained by adopting percoll density gradient centrifugation, and the purpose of abdomen borrowing reproduction is achieved by adopting a microinjection mode, so that the preservation of germ cells of endangered fishes and the reconstruction of artificial populations are realized; and a new technical approach is provided for protecting genetic resources of the Chinese sturgeons and breaking through artificial propagation bottlenecks.

The invention discloses a Weissella virida 38-3 strain and an application thereof. Relates to the technical field of microorganisms. The invention provides a strain and application thereof. The strain is safe in separation source, high in growth speed and excellent in fermentation performance; the strain has the advantages of good biological safety, no hemolytic activity and no generation of bioamine, is sensitive to antibiotics such as ampicillin, erythromycin, kanamycin, streptomycin, tetracycline, vancomycin, chloramphenicol, gentamicin, clindamycin, ciprofloxacin, cefixime and the like, has good probiotic performance, has good gastric juice and intestinal juice tolerance and strong cell surface adhesion, and can be used for preparing antibiotics. Good pathogenic bacterium inhibiting and cholesterol lowering capabilities are realized. The strain disclosed by the invention can be used as a probiotic to prepare a food leavening agent, multifunctional medicines, functional foods or health-care foods, and has wide market prospects and application values.

The invention relates to the technical field of culture media, in particular to a serum-free complete culture medium for human umbilical cord mesenchymal stem cells and a preparation method thereof.The serum-free complete culture medium takes DMEM / F12 as a basic culture medium and is matched with UltraGRO-Advanced, a penicillin-streptomycin double-antibody solution, a recombinant human epidermal growth factor and a basic fibroblast growth factor, and the serum-free complete culture medium for the human umbilical cord mesenchymal stem cells is obtained. The serum-free complete culture medium can replace a serum culture medium in a conventional culture system of mesenchymal stem cells, the characteristics of normal adherence, good growth state and stem cells of the cells are kept, the serum-free complete culture medium reduces the risks of heterologous contamination and immunological rejection to the maximum extent, the added components capable of promoting cell growth and the content can be determined and controlled, and the culture medium is suitable for large-scale popularization and application. The use is safer.

The invention relates to a phenylacetylglutamine / phenylacetylglycine induced vascular smooth muscle cell senescence model and an establishment method and application thereof, and belongs to the technical field of cell models.The method comprises the following steps that vascular smooth muscle cell suspension is placed in a porous plate, 10% of FBS and 1% of mycillin are added into a culture medium, 24 h after cells adhere to the wall, the vascular smooth muscle cell senescence model is obtained, and the vascular smooth muscle cell senescence model is obtained. The method comprises the following steps of: adding a proper amount of phenylacetyl glutamine or phenylacetyl glycine dimethyl sulfoxide solution into a porous plate, changing a serum-free culture medium, continuously culturing for 24 hours, adding a proper amount of phenylacetyl glutamine or phenylacetyl glycine dimethyl sulfoxide solution into the porous plate, changing the culture medium added with phenylacetyl glutamine or phenylacetyl glycine once every 24 hours, and collecting cells after 72 hours. According to the invention, phenylacetylglutamine / phenylacetylglycine is used for inducing and establishing the vascular smooth muscle cell senescence model for the first time, the method is easy to operate and low in cost, and a foundation is laid for researching the relationship between cardiovascular diseases and senescence.

The invention discloses an anti-streptomycin monoclonal antibody and application thereof, and relates to the technical field of streptomycin detection. According to the invention, a hybridoma cell strain capable of stably secreting an anti-streptomycin monoclonal antibody is screened, and the preservation number of the hybridoma cell strain is CGMCC (China General Microbiological Culture Collection Center And screening to obtain the specific and sensitive streptomycin monoclonal antibody. Immunodetection experiments prove that the streptomycin monoclonal antibody provided by the invention has high specificity to streptomycin, high detection sensitivity to streptomycin and wide linear detection range. Therefore, the anti-streptomycin monoclonal antibody provided by the invention can be used for developing streptomycin detection kits, test strips and other products, and a detection tool and means are provided for rapid detection of streptomycin and other antibiotic residues in animal products, aquatic products and agriculture.

The present invention belongs to the technical field of semen cryopreservation, and more specifically, relates to sheep semen freezing solution and semen preservation method. The sheep semen freezing solution components of the present invention include Tris, citric acid, fructose, lactose, trehalose, penicillin, streptomycin, soy lecithin, nobiletin, glycerol and water. By using a specific content of nobiletin as an effective antioxidant component of a sheep sperm cryopreservative, it plays an antioxidant role when the sperm is frozen, making the sperm more stable during the cryopreservation process. After adding nobiletin, the vitality and motility of sheep sperm after freezing and thawing can be significantly improved. It effectively reduces the oxidative stress damage of sperm during the freezing process and improves the antioxidant capacity of sperm.

The invention aims to provide a preparation method of amnion-derived mesenchymal stem cells with high collagen expression. The preparation method comprises the following steps: step 1, reagents and materials: amnion tissues: obtaining a fresh amnion from a healthy puerpera after delivery; the PBS is used for cleaning amniotic tissues; collagenase: type I collagenase, which is used for digesting amniotic tissues to separate mesenchymal stem cells; a cell culture medium is a DMEM / F12 culture medium, and 10% of fetal calf serum and 1% of penicillin-streptomycin double antibodies are added; the transforming growth factor TGF-beta is used for promoting the expression of mesenchymal stem cell collagen; and the fibroblast growth factor FGF can assist in promoting cell proliferation and collagen expression. The invention aims to provide the preparation method of the amniotic membrane-derived mesenchymal stem cells for highly expressing collagen, and the amniotic membrane-derived mesenchymal stem cells can be widely applied to treatment of immune system diseases, reproductive system diseases, skin system diseases, skeletal system diseases, nervous system diseases, digestive system diseases, respiratory system diseases, circulatory system diseases and urinary system diseases. Particularly, the effects of immunosuppression and tissue repair are achieved.

The invention provides an NK (Natural Killer) cell culture medium, which is prepared by adding the following components into a 1640 culture medium: 8 to 11 percent v / v fetal calf serum, 18 to 22 mg / mL of recombinant human transferrin, 40 to 60 mu M of 1, 3-dicaffeoylquinic acid, 25 to 35 mu M of aceglutamide, 900 to 1100 IU / mL of human recombinant IL-2, 45 to 55 IU / mL of human recombinant IL-15, 45 to 55 IU / mL of human recombinant IL-21, 50 to 200 U / mL of penicillin, 50 to 150 mu g / mL of streptomycin and 1 to 2 mg / mL of more than 95 percent of polyporus gossypii polysaccharide. The NK cell culture medium provided by the invention can effectively improve the proliferation speed of NK cells and the killing effect on various tumor cells.

This invention discloses a method for directed evolution of antibiotic-resistant Escherichia coli strains based on cytidine deaminase, belonging to the field of microbial directed evolution and genetic engineering technology. This method uses E. coli as the host, introducing a recombinant plasmid expressing an optimized double-stranded cytidine deaminase mutant. Utilizing the low toxicity and high mutagenicity of this mutant, continuous passage evolution is carried out under gradient concentrations of aminoglycoside antibiotics (kanamycin and streptomycin). Combined with whole-genome sequencing, molecular docking, and reverse genetics verification, the A145T missense mutation in the wcaE gene is identified as the core functional site. This invention overcomes the shortcomings of traditional spontaneous and chemical mutagenesis, which suffer from low efficiency and significant strain damage. The mutation type is controllable, the evolutionary cycle is short, and the obtained engineered strains can tolerate up to 300 mg / L kanamycin while exhibiting streptomycin cross-resistance, and the genetic stability of the tolerance trait is strong. This method is simple, highly reproducible, and suitable for industrial breeding of stress-resistant E. coli, and can be widely applied in antibiotic fermentation, industrial microbial culture, and other scenarios.

The invention provides a goat semen cryopreservation solution. The goat semen cryopreservation solution comprises melatonin and one or more of sodium citrate, fructose, yolk, glycerol, penicillin, streptomycin and tris (hydroxymethyl) aminomethane. The cryopreservation liquid is used for preserving goat semen and can improve the pregnancy rate of long-term infertility ewes.

The invention relates to the field of stem cell biology, and discloses a culture solution and a method for differentiating human pluripotent stem cells into natural killer cells. The culture solution comprises a DMEM (Dulbecco Modified Eagle Medium), an F12 culture medium, an APEL blood cell culture medium, an induction factor, a chemotactic factor CXCL12, L-ascorbic acid, L-glutamine, beta-mercaptoethanol, sodium selenite, ethanolamine and penicillin-streptomycin. The invention also discloses a more efficient differentiation method for inducing the human induced pluripotent stem cells to differentiate into the NK cells, and the differentiation method provided by the invention can rapidly, efficiently, simply and conveniently induce the pluripotent stem cells to differentiate into the natural killer cells at low cost based on a culture medium with definite components and an optimized cell factor combination.

The invention discloses a culture medium for preparing a human-derived Merkel cell model and a construction method, and relates to the technical field of biologication.The key points of the technical scheme are that the culture medium comprises a culture medium E, and the culture medium E is formed by adding PRCi into an OMM culture medium; the OMM is prepared by adding GlutaMax (GlutaMax), B-27 (B-27) Minus, N2 (N2) suppliers, Penicilin-Streptomycin and 2-Mercaptoethanol into an Advanced DMEM (Dulbecco's Modified Eagle Medium) / F12 (Fullcap 12) culture medium and a Neurobasic culture medium, and the OMM is prepared by adding GlutaMax, B-27 Minus, N2 (N2) suppliers, Penicilin-Streptomycin and 2-Mercaptoethanol into a medium; and the PRCi comprises EED226, PRT4165 and GSK126 (glutamine synthase 126). According to the invention, a small molecule compound combination PRCi (EED226 + PRT4165 + GSK126) is added into a culture medium, so that the amount of MC in the organoid can be remarkably increased, and a human-derived system is established for researching the MC.

The invention belongs to the technical field of microbial pharmacy, and discloses a polyketone macrolide compound generated by genetically engineered streptomyces as well as a preparation method and application thereof. The polyketone macrolide compound disclosed by the invention is a cinnamyl streptomycin derivative, has cell proliferation inhibition activity better than that of a natural product Cinnamomycin A-D, can exert anti-tumor activity by inhibiting activity of human exonucleotide pyrophosphatase / phosphodiesterase 1 (ENPP1) and activating an inherent immune pathway, can be prepared into a medicine or a medicinal composition, and can be used for preparing medicines or medicinal compositions. The compound is used for treating tumors and related diseases.

The invention relates to a Zhongshengmycin production strain and application of the Zhongshengmycin production strain, the strain is streptomyces sp. ZS-2, the Zhongshengmycin production strain has good Zhongshengmycin production activity, and a fermentation product has higher bacteriostatic activity and a wider bacteriostatic range.

The invention discloses a culture medium and a culture method of a breast cancer organoid. The culture medium comprises an Advanced DMEM / F12 basal culture medium and an additive, the additive is prepared from penicillin streptomycin / amphotericin B, a B-27 additive, L-glutamine amide, HEPES (Hydroxyethyl PES), N-Acetylcysteine, nicotinamide, SB202190, A83-01, FGF10 (Fibroblast Growth Factor 10), EGF (Epidermal Growth Factor), human Hergulin beta-1, Y27632, WNT-3A, R-spondin 1, NOGGIN (Nitrogen Growth Factor IN), FGF7 (Fibroblast Growth Factor 7) and beta-estradiol. The culture method of the breast cancer organoid comprises the step of using the breast cancer organoid culture medium, and the method specifically comprises the steps of tissue sample taking, primary tissue construction, breast cancer organoid liquid changing and breast cancer organoid subculture. According to the prepared breast cancer organoid culture medium, tumor cells taken from breast cancer tumors grow fast, the cell speed is high, and follow-up medicine screening experiments can be facilitated; after the breast cancer organoid culture medium is used for culturing the breast cancer organoid, the cellular morphology and the protein expression condition of the organoid are identified through HE and immunohistochemistry, and it can be seen that the organoid can well reduce the pathological characteristics of the tissue.

The invention relates to an isolated culture method of functional Chinese softshell turtle ovarian granular cells. The isolated culture method comprises the following steps: S1, collecting ovarian tissues: collecting Chinese softshell turtle ovarian tissues; s2, separating and collecting a granular cell layer; s3, digesting and collecting granular cells; s4, primary culture of granulosa cells: transferring the collected cells into a cell culture bottle containing 4 ml of a complete culture medium for primary culture, wherein the complete culture medium comprises the following main components: a DMEM culture medium, 15% of fetal calf serum, 1% of penicillin streptomycin and 1% of an insulin-transferrin-selenium additive; and S5, subculturing the granular cells. The isolated culture method and the culture medium system provided by the invention are simpler and more time-saving and labor-saving; according to the method, the aromatase CYP19A1 can be expressed normally, the characteristics of the granular cells are kept, the aromatase CYP19A1 can be expressed normally, it is indicated that the in-vitro cultured cells can keep the functionality of the cells, and the in-vitro proliferation capacity of the granular cells is improved to a great extent.