Rosaceae and prunusoidea cross-genus cross-breeding method

A technology of hybrid breeding and Rosaceae, applied in the field of plant hybridization, to achieve the effect of increasing success rate, reducing technical threshold and operation difficulty

Inactive Publication Date: 2020-12-11
禄劝普林生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on this kind of cultivation methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for cross-genus cross-breeding of Rosaceae Lisubaceae, comprising the steps of:

[0024] (1) Pollination solution preparation:

[0025] Taking corresponding substances to prepare pollination liquid for subsequent use, the pollination liquid contains EDTA, boric acid, cytokinin and brassin;

[0026] (2) Pollen processing and artificial pollination:

[0027] Spray the pollination solution prepared in step (1) onto the pistil, irradiate the pollen with 254nm ultraviolet rays for 15 minutes, and then carry out artificial pollination;

[0028] (3) Bagging breeding:

[0029] Carry out bagging to the flower after artificial pollination, take out seed for subsequent use until breeding fruit;

[0030] (4) Microbody cultivation:

[0031] Carry out microbody cultivation to the seed of step (3) gained, obtain grafting branch for subsequent use;

[0032] (5) Grafting management:

[0033] Graft the grafted shoots obtained in step (4) to a compatible rootstock, and then...

Embodiment 2

[0038] A method for cross-genus cross-breeding of Rosaceae Lisubaceae, comprising the steps of:

[0039] (1) Pollination solution preparation:

[0040] Taking corresponding substances to prepare pollination liquid for subsequent use, the pollination liquid contains EDTA, boric acid, cytokinin and brassin;

[0041] (2) Pollen processing and artificial pollination:

[0042] Spray the pollination solution prepared in step (1) onto the pistil, irradiate the pollen with 254nm ultraviolet rays for 15 minutes, and then carry out artificial pollination;

[0043] (3) Bagging breeding:

[0044] Carry out bagging to the flower after artificial pollination, take out seed for subsequent use until breeding fruit;

[0045] (4) Microbody cultivation:

[0046] Carry out microbody cultivation to the seed of step (3) gained, obtain grafting branch for subsequent use;

[0047] (5) Grafting management:

[0048] Graft the grafted shoots obtained in step (4) to a compatible rootstock, and then...

Embodiment 3

[0053] A method for cross-genus cross-breeding of Rosaceae Lisubaceae, comprising the steps of:

[0054] (1) Pollination solution preparation:

[0055] Taking corresponding substances to prepare pollination liquid for subsequent use, the pollination liquid contains EDTA, boric acid, cytokinin and brassin;

[0056] (2) Pollen processing and artificial pollination:

[0057] Spray the pollination solution prepared in step (1) onto the pistil, irradiate the pollen with 254nm ultraviolet rays for 15 minutes, and then carry out artificial pollination;

[0058] (3) Bagging breeding:

[0059] Carry out bagging to the flower after artificial pollination, take out seed for subsequent use until breeding fruit;

[0060] (4) Microbody cultivation:

[0061] Carry out microbody cultivation to the seed of step (3) gained, obtain grafting branch for subsequent use;

[0062] (5) Grafting management:

[0063] Graft the grafted shoots obtained in step (4) to a compatible rootstock, and then...

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PUM

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Abstract

The invention discloses a rosaceae and prunusoidea cross-genus cross-breeding method, and belongs to the technical field of plant hybridization. The method comprises the following steps of (1) pollination liquid preparation, (2) pollen treatment and artificial pollination, (3) bagging breeding, (4) microsome cultivation, and (5) grafting management. According to the method, through application andreasonable management of the pollination liquid, compared with traditional natural hybridization, the success rate of pollination is greatly increased, a corresponding hybridized female parent is obtained, and compared with a somatic cell fusion breeding technology, the technical threshold and the operation difficulty are reduced, so that the method is more feasible in subsequent popularization and application.

Description

technical field [0001] The invention belongs to the technical field of plant hybridization, and in particular relates to a cross-genus cross-breeding method of the Rosaceae Prunaceae. Background technique [0002] Due to the problems of reproductive isolation and hybrid sterility, distant hybridization has slowed the progress of related scientific research. At the same time, it is becoming the mainstream cultivation and breeding category in countries such as Europe, America and Australia. At present, there are few studies on such cultivation methods in our country, and continuous exploration and improvement are needed. Contents of the invention [0003] The purpose of the present invention is to provide a method for intergenus cross-breeding of Rosaceae Pruninaceae. [0004] Above-mentioned technical purpose of the present invention is achieved through the following technical solutions: [0005] A method for cross-genus cross-breeding of Rosaceae Lisubaceae, comprising t...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01G2/30A01N43/90A01N43/22A01N59/14A01N37/44A01P21/00
CPCA01H1/02A01G2/30A01N43/90A01N43/22A01N59/14A01N37/44
Inventor 谢伟明黄嘉威李志钧
Owner 禄劝普林生物科技有限责任公司
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