Process for abstracting root protoplast of rosaceous plants
A technology of protoplasts and Rosaceae, which is applied in the field of extracting root protoplasts of Rosaceae plants, can solve problems such as wasting time, increasing the number of ruptures, and rupture of protoplasts, and achieves the effect of great application prospects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0018] Embodiment 1, the cultivation of material
[0019] In this example, salt-tolerant Malus zumi Mats were used as experimental materials.
[0020] The media used are as follows:
[0021] (1) MS medium:
[0022] Element
molecular weight
Use concentration (mg / L)
Macronutrients
Potassium nitrate KNO3
Ammonium nitrate NH4NO3
Potassium dihydrogen phosphate KH2PO4
Magnesium Sulfate MgSO4.7H2O
Calcium chloride CaCl2.2H2O
101.11
80.04
136.09
246.47
147.02
1900
1650
170
370
440
trace elements
Potassium iodide KI
Boric acid H3BO3
Manganese Sulfate MnSO4.4H2O
Zinc sulfate ZnSO4.7H2O
Sodium molybdate Na2MoO4.2H2O
Copper Sulfate CuSO4.5H2O
Cobalt chloride CoCl2.62
166.01
61.83
223.01
287.54
241.95
249.68
237.93
0.83
6.2
22.3
8.6
0.25
0.025...
Embodiment 2
[0037] Embodiment 2, extract root protoplast
[0038] 1. Preparation of enzymatic hydrolysis solution
[0039] Enzyme base solution: 2mmol / LCaCl 2 , 1mmol / L MgCl 2 , 0.05mg / mL bovine serum albumin (BSA), 0.044mg / mL ascorbic acid (VC), 0.5mg / mL polyvinylpyrrolidone (PVP), and the rest is water.
[0040] Enzyme solution I: Add Cellulysin (Shanghai Beiji Biotechnology Co., Ltd., XS-16010) to the enzyme base solution, so that the concentration of cellulase is 280U / ml enzymatic solution, and adjust the enzymatic solution The pH value is 5.8. The enzymatic solution was sterilized by filtration through a 0.22 μm mixed cellulose pore filter.
[0041] Enzymolysis solution II: add cellulase RS (Cellulase RS) (Yakult Company, L0011) to the enzyme base solution; and pectinase Y-23 (Pectolase Y-23) (Shanghai Maikun Chemical Co., Ltd., 25M0460), The concentration of cellulase RS is 240U / ml enzymolysis solution, the concentration of pectinase Y-23 is 2U / ml enzymolysis solution, and the ...
Embodiment 3
[0051] Embodiment 3, the extraction of protoplast
[0052] 1. Preparation of enzymatic hydrolysis solution
[0053] Enzyme base solution: 0.5mmol / LCaCl 2 , 0.5mmol / L MgCl 2 , 0.15mg / mL bovine serum albumin (BSA), 0.022mg / mL ascorbic acid (VC), 0.25mg / mL PVP, and the rest is water.
[0054] Enzyme solution I: Add Cellulysin (Shanghai Beiji Biotechnology Co., Ltd., XS-16010) to the enzyme base solution, so that the concentration of cellulase is 210U / ml enzymatic solution, and adjust the enzymatic solution The pH value is 5.8. The enzymatic solution was sterilized by filtration through a 0.22 μm mixed cellulose pore filter.
[0055] Enzymolysis solution II: add cellulase RS (Cellulase RS) (Yakult company, L0011) to the enzyme base solution; and pectinase Y-23 (Pectolase Y-23) (Shanghai Maikun Chemical Co., Ltd., 25M0460), The concentration of cellulase RS is 80 U / ml enzymolysis solution, the concentration of pectinase Y-23 is 1 U / ml enzymolysis solution, and the pH value of ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap