The invention discloses a preparation method and application of
plant-derived extruded nano-vesicles, and relates to the technical field of biological medicines. The preparation method of the
plant-derived extruded nano-vesicles comprises the following steps: juicing cleaned
plant roots, stems, leaves, flowers or fruits to obtain plant juice, and filtering with gauze to obtain first filtrate; centrifuging the first filtrate at a
low speed of 300-6000 * g to obtain a second filtrate; performing 8,000-20,000 * g
centrifugation on the second filtrate for 0.5-3 hours at the temperature of 0-5 DEG C, so as to obtain a first precipitate; adding the first precipitate into a
buffer solution, carrying out ultrasonic treatment, diluting, and sequentially passing through a filter membrane with the
pore diameter of 0.4-10 microns and a filter membrane with the
pore diameter of 0.1-0.4 microns, so as to obtain a third filtrate; and centrifuging the third filtrate, taking the precipitate, and resuspending to obtain the extruded nano-vesicles. Experimental detection shows that the extruded vesicles are similar to natural vesicles in characteristics, but the yield is far higher than that of the natural vesicles, and the extruded vesicles can serve as nano-carriers to be applied to a foundation or clinic.