The invention relates to a leaf vegetable organic fertilizer and a preparation method thereof. The preparation method comprises the steps of grinding maize straws, wheat straws, soybean straws and sesame straws into straw particles according to a mass ratio of 3:3:2:2, pretreating the straw particles by adding water and ammonium hydroxide to obtain a mixture A; carrying out ultraviolet ray treatment on the taken duck manure and duck feathers, and then carrying out ozone treatment, thus obtaining a mixture B; adding aspergillus niger and saccharomycetes into a mixture of grinded manioc wastes, grinded kelp wastes and water, and fermenting to obtain a mixture C; mixing the mixtures A, B and C, shell powder, plant ash and sodium humate, and adding EM (Effective Microorganism) for fermentation to obtain a mixture D; and adding bone meal and charcoal dust into the mixture D, then carrying out ozone sterilization, drying, charging in a separating manner, and carrying out ultraviolet sterilization to obtain the leaf vegetable organic fertilizer. The prepared leaf vegetable organic fertilizer contains various nutritional ingredients needed by the growth of leaf vegetables, regulates soil, activates the activity of microorganisms in the soil, overcomes soil hardening, and improves the permeability of soil air.