126 results about "Frond" patented technology

The invention discloses a composite type artificial algal reef comprising a mature algal reef and a seedling reef. The mature algal reef comprises a solid base. The upper surface of the solid base is embedded with a metal frame, wherein the shape of the metal frame is identical to that of the upper surface of the solid base and the size of the metal frame is identical to that of the upper surface of the solid base. A conch net bag is arranged in the metal frame. The center of the upper surface is embedded with a metal stand column. A steel ring is welded to the upper end of the stand column. A plurality of seedling ropes are fixed between the metal frame and the steel ring. The seedling reef is fixed to the side face of the solid base of the mature algal reef, and algal seedlings are attached to the seedling reef. By the adoption of the composite type artificial algal reef, the attaching effect of alga is guaranteed, the repair period of an algae field is shortened, the success rate of repair and reconstruction of the algae field is improved, the adhesive rate of the seedlings is increased, and the seedling reef is firm, durable, small in size, light, and convenient to carry; the net bag made of waste conches is placed in the metal frame, and a biophile reef can be composed through diffusion and attachment of spores of fronds on the seedling ropes; resource utilization of the waste conches is achieved, and composite type artificial algal reef has the advantages of being economical and easy and convenient to use.

The invention provides a method for extracting astaxanthin from haematococcus pluvialis, and belongs to the technical fields of bioprocess technology and chemical technology. The method comprises the following steps: completing the selection of raw materials through abnormal growth of cells of the selected haematococcus pluvialis, dilution of high-density algae cells and light induction, carrying out wall-breaking treatment on frond by using an ultrahigh pressure extractive technology, then extracting, eluting, concentrating, saponifying and centrifuging, thus obtaining the astaxanthin. The method has the advantages that the consumption of an organic solvent is little, an extraction agent can be circularly utilized, the environmental pollution is little, the production cost is low, the recovery rate is high, few impurities exist, the possibility of oxidative degradation of the astaxanthin is reduced, and the extraction quality of the astaxanthin is improved.

The present present invention relates to a method of simultaneously extracting phycoerythrin and agar from seaweeds such as asparagus, etc in seaweeds biological chemical field. The concrete operation process is that cells of the asparagus frond are crushed. A phycobiliprotein crude extract is obtained from water soluble extracts after ammoniym sulfate precipitation. The crude extract is extracted by Phenyl-Sepharose expansion columns and phycoerythrin products are obtained. Q-Sepharose iron exchange columns are used to purify the phycoerythrin products to obtain purified phycoerythrin products. Then, the left asparagus residues after the phycoerythrin extract are used to extract the agar. The present invention can simultaneously obtain the phycoerythrin and the agar from the asparagus. And the purified phycoerythrin purity is larger than 2.954, and the yield is 0.097mg per gram of fresh algae. The yield of the obtained agar is 2.31percent (the algae is wetly weighted), which is equivalent to the yield of an agar direct extraction method. Therefore, the utilization ratio of the asparagus can be greatly improved by adopting the method.

The invention discloses a method for producing anoectochilus formosanus by tissue culture, comprising the following steps: frond selection and asepsis, cluster bud inducing culture and large-scale culture. The key to the technique is characterized in that two different culture media are applied to the inducing culture and the large-scale culture, wherein the inducing culture medium is as follows: H culture medium+ 10-50g/L of cane sugar + 0.1-3.0mg/L of alpha-naphthylacetic acid+ 0.1-5.0mg/L of 6-benzyladenine, and pH value is 5.0-7.0; the large-scale culture medium is as follows: B5 culture medium+ 20-40g/L of cane sugar+ 6-8g/L of agar+ 2.0-3.0g/L of active carbon+ 1.0-2.0mg/L of alpha-naphthylacetic acid+ 2.0-3.0mg/L of benzyladenine, and pH value is 5.5-6.5. The method is easy to operate, features low production cost, high output, high quality and no pollution to the environment and can realize batch production.

The present invention provides an introduction method of the laminaria japonica, which can resolve the problems of the prior art that the selected frond section having the mature sporangia is easy to decay and the spore is easy to release, thereby leading to the failure of the introduction. The introduction method adopted by the present invention is to firstly dry and incite the mature sporangia leaf of the laminaria japonica in a temperature-controllable laboratory, then put the mature sporangia leaf of the laminaria japonica into a water tank that is provided with the fresh sterilized sea water and the temperature of the water is controlled between eight cent degrees and twelve cent degrees to release the spores, then cultivate the spore to form the gametophyte, to put the seedling curtain attached with the gametophyte into a low-temperature enclosed bag and finally transport in low-temperature storage to the destination. The shape of all gametophytes on the laminaria japonica seedling curtain is normal, which has high successful rate and the survival rate is nearly one hundred percentages.

A cell culture process for flagelliforme includes such steps as separating the cells from flagelliforme frond to obtain high-activity flagelliforme cells, culturing them in culture medium to obtain its seed cells, storing them at 8-12 deg.C under 500 lux or freeze drying for long-term storage, large-scale culturing to obtain the cultured product, and extracting flagelliforme polyol.

The invention relates to a gulfweed pond culture facility and an application method, in particular to a simple raft frame for culture of gulfweed, which can solve the problem of being short of pond culture and gulfweed facilities. The facility consists of a cable, floats, a floating rope, a seeding rope and a dropstone, wherein the cable is used for towing the floating rope and keeping the floating rope straight, the floats are connected at two ends of the floating rope for producing buoyancy and indicating the positions, the seeding rope is fastened on the floating rope for fixing gulfweed, the buoyancy of the gulfweed is used for leading the floating rope to be in the floating state, and the length of an extension rope of the dropstone is regulated to leading the floating rope to be in the appropriate water depth. The facility has simple structure and low cost, and can lead a frond to be uniformly distributed in a water body with the help of the buoyancy of the gulfweed, thereby fully utilizing space of the water body, improving the growth efficiency and facilitating the management and the harvesting. The utilization of the facility for the culture of the gulfweed can effectively purify water quality while improving the growth efficiency of the frond, provide shelters for fish and shrimps, and lead the cultured products of the gulfweed to have higher economic value.

The invention provides nostoc capable of degrading polychlorinated biphenyls. A frond of the nostoc is multicellular protonema, has algal filaments and a colloid sheath, and can fix inorganic nitrogen. The nostoc can degrade polychlorinated biphenyl (PCB) 1254 efficiently; after the nostoc is exposed for a week, the degradation efficiency of the nostoc on the PCB1254 is over 60 percent, and the content of residual chlorine ions tested in a culture medium after the degradation and dechloridation of the PCB1254 is 2.26mg/L and 3.01mg/L; the one-month degradation efficiency of the nostoc on six typical indicative polychlorinated biphenyl (PCB52, PCB101, PCB118, PCB138, PCB137 and PCB180) in environment is more than 70 percent, wherein the degradation rate of the PCB52 is 98.7 percent; and after the PCB1254 is degraded for one month, the residual amount of the PCB1254 in the culture medium is only 15.65 percent. Therefore, the nostoc can be widely applied to the degradation of the PCBs insoil environment.

A semiconductor device according to one embodiment includes: a semiconductor substrate; an element isolation insulating film embedded in the vicinity of a front surface of the semiconductor substrate; a through plug penetrating the semiconductor substrate from a back surface to the front surface so as to penetrate through the element isolation insulating film, and having a multi-stage structure comprising an upper stage portion and a lower stage portion, the upper stage portion having a region surrounded by the element isolation insulating film in the semiconductor substrate, the lower stage portion having a diameter larger than that of the upper stage portion; and a contact plug connected to an end portion of the through plug on the frond surface side of the semiconductor substrate for connecting a conductive member formed above the front surface side of the semiconductor substrate to the through plug.

The present invention provides a sargassum thunbergii seed-vegetable artificial-ripening condition controlling method, which can resolve the problems that the maturation of the male seed vegetable and the female seed vegetable is not synchronous and the developing time of the homosexual seed sargassum thunbergii in the sexual reproduction breeding process. The present invention uses the conditions that can be artificially controlled to achieve the artificial ripening of the seed vegetable in the seedling breeding process and can provide an excellent-quality seed vegetable for the seedling of the sargassum thunbergii. The technical proposal of the present invention comprises the selection of the adult sargassum thunbergii, gripping of the adult sargassum thunbergii, the selection of the temporary-cultivation pool and the controlling of the conditions for cultivation. Adopting the method of the present invention, the lighting time on the adult sargassum thunbergii is prolonged, the lighting strength is increased, the influence of the severe environment on the frond is reduced, the maturation of the pyrenium is relatively concentrated, the maturation of the male and the female pyrenium becomes synchronous and a plenty of concentrated fertilized eggs are achieved, which is beneficial for the success of the one-off seedling collection.

The invention discloses an artificial ecological culturing method for caulerpa lentillifera. The method comprises the following steps that filtered seawater is injected into a culture pond after the culture pond is completely cleared and exposed to the sun, and the seawater height is 1-1.2 m; healthy fronds which are not discolored or rotted are selected to be fully cleaned and then put into a double-layer clamp net after impurity removal, clean fine sand is laid under the clamp net to serve as a rhizoid attaching base, and the caulerpa lentillifera is put at about 300 grams/square meter; all indexes including water temperature, salinity, PH, COD, illumination, transparency, PO4<3+>-P and NO3<+>-N of the water quality are controlled in the culturing process, and micro air inflation is performed in the whole culturing process; bait bags are hung at a water head, the periphery of the culture pond and the periphery of an air head in the culturing process. According to the method, the defects that the nutrient substance variety in a culturing environment is single and the caulerpa lentillifera is low in unit yield, poor in frond color and more in hybrid algae due to the fact that artificial mixed feed is fed can be overcome, the water quality of a natural sea area cannot be polluted, and seawater eutrophication is effectively inhibited.

The invention provides a connection block which comprises a front connecting rod (1) and a shaft locator frond end (2) arranged at one end of the front connecting rod (1), wherein, the shaft locator frond end (2) is movably connected with one end of the front connecting rod (1). According to the invention, the front connecting rod of the connection block is movably connected with the shaft locator frond end through a pin, and therefore, the advantages of a simple structure and fixed and firm connection are achieved.

A vertical axis wind turbine apparatus can be implemented as a support structure, atop which is located a rotatable domed housing for a blade assembly. The blade assembly includes curved blades vertically attached to a drive shaft which can either turn a magnetic rotor or be attached directly to a generator in order to produce electricity. The blade assembly receives wind through both a main inlet and a secondary inlet and expels wind through an exhaust duct. The device can be constructed from carbon fiber to prevent damage from lightning. The device is also constructed with the appearance of a tree where the support structure resembles a tree trunk and panels resembling palm fronds or branches can be attached to the domed housing. The design creates an efficient and aesthetically pleasing wind turbine that can be utilized in both residential and commercial applications.

The invention discloses a grading method of a humic acid large-pore-size resin in frond. The graded extraction method comprises the following steps: frond pretreatment, Soxhlet extraction of frond, calcium chloride treatment of frond, sodium carbonate treatment of frond, alkali solution extraction of frond, frond humic acid silicon removal, frond humic acid fulvic acid removal, subcomponent grading of frond humic acid, and desalinization and solidification of frond humic acid subcomponent. The method can extract humic acid from the brown alga organism and perform subcomponent separation by using an XAD-8 resin separation technique, thereby finally obtaining the graded frond humic acid subcomponent.

The children's cough relieving mixture with combined cough relieving and fever allaying functions is prepared with honeysuckle, Japanese felt fern frond, burdock fruit, isatis leaf, wolfberry bark and other five kinds of Chinese medicinal materials. The preparation process includes distilling partial material to collect distillate, decocting other medicine materials, filtering, concentrating filtrate, alcohol precipitation, concentrating supernatant, water precipitation, filtering supernatant, concentration, preparing syrup with cane sugar, merging, adding benzoic acid to regulate specific weight, sterilizing and other steps.

The invention discloses a preparation method of a nostoc commune culture medium. The preparation method comprises the following steps of: by taking green plant corncobs convenient to obtain, wide in source and capable of storing drained water and nutrients as raw materials according to the biological characteristics of nostoc commune, burdening, soaking a nutrient solution and drying to prepare the nostoc commune culture medium which is optimized, rich in nutrient substances required for adsorption growth of nostoc commune, ventilatory, water-permeable, low in production cost and pollution-free. The culture medium is rod-shaped. When the culture medium is applied, a single layer of culture medium is distributed and arranged on a culture bed; during seeding, nostoc commune frond can be directly spread on the culture medium, thus a new safe and effective material which is convenient to update and change after harvesting is provided for implementing artificial soiless high-yield cultivation inside and outside a nostoc commune room. As the nostoc commune culture medium is rough and uneven in surface, the nostoc commune culture medium has a very good preventive effect on cultivation destructive disease waterlogging nostoc commune rotting often caused by common insect pests such as slugs and snails in nostoc commune and poor water drainage in an underlying surface.

The invention discloses a grading extraction method of humic acid in frond. The grading extraction method includes the following steps that frond preprocessing, soxhlet extraction of the frond, calcium chloride and acid pickling of the frond, sodium carbonate processing of the frond, alkali liquor extraction of the frond, silicon removal of frond humic acid, fulvic acid removal of frond humic acid and desalting and solidifying of frond humic acid. By means of the mode, the humic acid can be extracted from the frond, subfraction separation is conducted through an XAD-8 resin separation technology, and a graded frond humic acid subfraction is finally obtained.

The invention discloses a construction and transformation method of a chamomile farnesyl diphosphate synthase (FPS) gene eukaryotic expression vector, comprising the steps of: extracting an RNA (ribonucleic acid) from chamomile petals, and inversely transcribing the RNA into cDNA (complementary deoxyribonucleic acid) with total chamomile RNAs as a template; carrying out PCR (Polymerase Chain Reaction) amplification using a cDNA as a template to obtain a target fragment; connecting the target fragment with a T4 ligase; transforming a connection product to an escherichia coli DH5 alpha competent cell to obtain an eukaryotic expression vector PBI121/FPS; then introducing a recombinant expression vector to agrobacterium tumefaciens EHA105; and infecting the plant Yunyan85 by using the agrobacterium tumefaciens EHA105 to finally obtain a resistant tobacco plant. An FPS gene is cloned by using a gene engineering technology, the callus tissues of the plant are infected by virtue of the agrobacterium tumefaciens, and the construction and transformation method lays a foundation for improvement of chamazulene yields through expression of the FPS gene in a plant frond.

The invention discloses a steering wheel device for steer-by-wire vehicles, and aims to accurately detecting the rotary angle of a steering wheel, providing a proper and adjustable road feeling for a driver, changing the work modes under different work conditions and adjusting the full-steering rotary turn numbers of the steering wheel. A driving shaft is fixedly connected with an input shaft of an angular displacement sensor by a coupler, a transmission nut and a screw shaft are mounted in a match and transmission mode, a return spring is fixedly connected with the transmission nut by a support, an electromagnet is fixedly connected with the transmission nut, an iron core of the electromagnet and a baffle are connected by a pin shaft, grooves with different lengths are arranged on the frond and rear sides of a stroke baffle, the transmission nut is fixedly connected with a linkage rod, a work oil port of an oil cylinder is correspondingly connected with a work oil port of a proportional throttle valve by a pipeline, and a differential pressure sensor is arranged between two work oil pipelines of the oil cylinder. The steering wheel device for the steel-by-wire vehicles is suitable for engineering vehicles to be used as the steer-by-wire steering wheels.

The invention discloses a chlorella Chlorella sp.HQ culture method and application in water purification and oil production, and belongs to the technical field of production of single cell oil and biodiesel. The oil-producing freshwater chlorella is named chlorella HQ and is stored with registration number as CGMCC No.7601. The culture method comprises the following steps of: inoculating the chlorella HQ strain in a logarithmic phase into secondary effluent of municipal sewage; controlling the chlorella inoculums density to be 5*10<4> to 5*10<5> mL<-1>, light-dark ratio to be (12:12)-(14:10), illumination intensity to be 40 to 60 micromole.(m2.s)-1, temperature to be 20 to 30 DEG C, and pH (Potential of Hydrogen) to be 6 to 9; culturing for 10 to 14 days under the conditions above; and separating and obtaining frond as well as collecting the purified water. The application of the chlorella HQ in water purification and oil production is characterized in that the secondary effluent of municipal sewage is used as a culture medium, and the chlorella HQ enables the pollutant in the secondary effluent to be continuously converted and proliferated in order to accumulate single cell oil, simultaneously the water is deeply purified, and the water resource is protected; and therefore, the culture method brings double benefit, and is environmentally-friendly, low in cost and beneficial for the industrial application of chlorella HQ by large-scale culture.

Preparation and application of a fused oral interferon expression frond for fowl are disclosed. On the basis of the conserved sequences of fowl interferon alpha and gamma genes, the interferon alpha gene having great antiviral effect and the interferon gamma gene capable of improving the immunity of the organism and enhancing the constitution of the organism are fused for expression, so that the expressed alpha+gamma fused interferon has the synergetic functions of the two. In the mean time, a dunaliella bioreactor is used as an expression system; apart from that the system is capable of expressing exogenous genes, own advantages such as rich nutrition, nontoxicity, harmlessness and the like of dunaliella are also utilized; consequently, a gene algae plant for multiple purposes is obtained; the fused oral interferon expression frond for fowl prepared from the genetically modified algae plant can be applied to the fields such as prevention or treatment of fowl viroses, fowl breeding, related feed processing industry and the like; and the fused oral interferon for fowl is good in effect, green and environment-friendly, and also low in cost.

The invention discloses a selecting and breeding method for high-quality hizikia fusiformis. The method includes the steps that firstly, ripe hizikia fusiformis fronds are selected as breeding vegetables, the quality components of the breeding vegetables are measured, and the breeding vegetables with the quality components meeting the following requirements are selected to be cultivated, wherein the protein content of the dry breeding vegetables is larger than or equal to 10%, and the sum of the proportions of threonine, cysteine, methionine, valine, isoleucine, leucine, tyrosine, phenylalanine, lysine and tryptophan is larger than or equal to 40% of the content of the hizikia fusiformis protein; secondly, after the breeding vegetables meeting the quality requirements are collected, the fronds on the lower portions of the breeding vegetables are removed, the fronds in the middle and on the upper portions of the breeding vegetables are kept, female seedlings and male seedlings are placed in a nursery pond in pairs to be cultivated for 5 days to 8 days, sperms and eggs are released, the eggs are fertilized to form young embryos, and the young embryos are collected. By means of the method, the breeding effect of hizikia fusiformis can be remarkably improved, the quality of hizikia fusiformis bred through the method is uniform, the breeding effect is good, the breeding vegetables can be randomly selected for breeding, and the breeding quality of the breeding vegetables is stable.

The invention provides a short-term cold storage method of a porphyra yezoensis cultivation net curtain, which comprises the following steps that: (1) the net curtain after porphyra yezoensis conchospore is seedling collected adopts a semi-floating raft to closely link and emerge the seedling; (2) the net is immediately disassembled before disease and mixed algae appear or when the disease and the mixed algae appear, the seedling net with uniform seedling emergence and strong frond is selected to be dried until the moisture content is 20 to 40 percent, florescence appears on the frond, and the frond has elasticity being pulled by hand; and (3) and then the net curtain is warehoused and cold stored to be used for the cultivation. The method has the advantages that: when unfavorable situations such as increasing of water temperature, disease and the like are discovered during the cultivation process, seedling net in the cultivation can be cold stored for a short term with the method so as to prevent the unfavorable situation of the sea area.

The invention belongs to the field of traditional Chinese medicines and provides a pharmaceutical preparation for treating pelvic inflammation. The pharmaceutical preparation is prepared from the following raw materials: 20 parts of campsis grandiflora, 16 parts of Indian azalea leaves, 15 parts of rough knotweed herbs, 20 parts of lobedfruit tacca leaves, 20 parts of fragile blueberry roots, 15 parts of serrateleaf carallia herbs, 18 parts of chun spicebush roots, 20 parts of common oxyspora roots, 16 parts of Chinese shuteria roots and 20 parts of okubo dryoathyrium frond. The pharmaceutical preparation can be used for treating pelvic inflammation.

An electrical generator using ocean wave and current forces to spin a turbine mean and alternator connected together by a flexible shaft. An electrical generator that takes its inspiration from a giant kelp plant that bends in wave or current forces thus presenting its turbine blades at the right angle to the force. A resilient flexible turbine generator of small stature resembling an ocean surf zone macrocystus which (instead of float and fronds) has a turbine floating at its top-most end and an alternator anchored to the ocean floor, both connected by a force transmitting flexible shaft. Ecologically friendly, turbo generator designed to be “ganged” into a “forest” of similar devices to harness the forces inherent in ocean waves and currents.