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Method for extracting astaxanthin from haematococcus pluvialis

A technology of Haematococcus pluvialis and astaxanthin, which is applied in the field of biological and chemical processes, can solve the problems of fast wear of high-pressure homogenizers and increased production costs, and achieve the goals of low environmental pollution, low production costs, and improved extraction quality Effect

Active Publication Date: 2014-05-14
QINGDAO KEHAI BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the high-pressure homogenization method has a high wall-breaking rate among various mechanical wall-breaking methods and can realize industrial production. However, the high-pressure homogenizer wears out quickly, which significantly increases the production cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step 1: High pressure extraction to break the algae

[0029] Broken algae: Weigh 10g algae, add 100mL soybean oil to the algae, pump it into a high-pressure vessel at a flow rate of 345mL / min, and perform wall-breaking treatment at a pressure of 100Mpa and a temperature of 4℃. Centrifugal separation to obtain astaxanthin extract and algae residue;

[0030] Step 2: Fast separation of astaxanthin

[0031] Extraction: Mix the astaxanthin extract and algae residue with silica gel at a weight ratio of 2:1, then add 5 mL ethanol and 15 mL ethyl acetate, stir and extract for 1 hour, then load the extraction mixture into the chromatography column;

[0032] Elution: Elute with ethanol and collect the red effluent part;

[0033] Concentration: Distill the red effluent under reduced pressure to obtain a dark red astaxanthin oily liquid.

[0034] Step 3: Crude extraction of astaxanthin oily liquid

[0035] Saponification: Mix astaxanthin oily liquid with 0.06mol / L potassium hydroxide-ethanol ...

Embodiment 2

[0041] Step 1: High pressure extraction to break the algae

[0042] Broken algae: Weigh 10g of algae, add 100mL soybean oil to the algae, pump it into a high-pressure vessel at a flow rate of 345mL / min, and perform wall-breaking treatment at a pressure of 200Mpa and a temperature of 4℃. Centrifugal separation to obtain astaxanthin extract and algae residue;

[0043] Step 2: Fast separation of astaxanthin

[0044] Extraction: Mix the astaxanthin extract and the algae residue with silica gel at a weight ratio of 3:1, then add 8mL ethanol and 18mL ethyl acetate, stir and extract for 2h, then load the extraction mixture into the chromatography column;

[0045] Elution: Elute with ethanol and collect the red effluent part;

[0046] Concentration: Distill the red effluent under reduced pressure to obtain a dark red astaxanthin oily liquid.

[0047] Step 3: Crude extraction of astaxanthin oily liquid

[0048] Saponification: Mix astaxanthin oily liquid with 0.06mol / L potassium hydroxide-ethanol...

Embodiment 3

[0054] Step 1: High pressure extraction to break the algae

[0055] Broken algae: Weigh 10g algae, add 100mL soybean oil to the algae, pump it into a high-pressure container at a flow rate of 345mL / min, and perform wall-breaking treatment at a pressure of 300Mpa and a temperature of 4℃. Centrifugal separation to obtain astaxanthin extract and algae residue;

[0056] Step 2: Fast separation of astaxanthin

[0057] Extraction: Mix the astaxanthin extract and algae residue with silica gel at a weight ratio of 3:1, then add 10mL ethanol and 20mL ethyl acetate, stir and extract for 3h, then load the extraction mixture into the chromatography column;

[0058] Elution: Elute with ethanol and collect the red effluent part;

[0059] Concentration: Distill the red effluent under reduced pressure to obtain a dark red astaxanthin oily liquid.

[0060] Step 3: Crude extraction of astaxanthin oily liquid

[0061] Saponification: Mix astaxanthin oily liquid with 0.06mol / L potassium hydroxide-ethanol so...

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Abstract

The invention provides a method for extracting astaxanthin from haematococcus pluvialis, and belongs to the technical fields of bioprocess technology and chemical technology. The method comprises the following steps: completing the selection of raw materials through abnormal growth of cells of the selected haematococcus pluvialis, dilution of high-density algae cells and light induction, carrying out wall-breaking treatment on frond by using an ultrahigh pressure extractive technology, then extracting, eluting, concentrating, saponifying and centrifuging, thus obtaining the astaxanthin. The method has the advantages that the consumption of an organic solvent is little, an extraction agent can be circularly utilized, the environmental pollution is little, the production cost is low, the recovery rate is high, few impurities exist, the possibility of oxidative degradation of the astaxanthin is reduced, and the extraction quality of the astaxanthin is improved.

Description

Technical field [0001] The invention relates to a method for extracting astaxanthin, in particular to a method for extracting astaxanthin from Haematococcus pluvialis, and belongs to the technical field of biology and chemical technology. Background technique [0002] Astaxanthin is a carotenoid with bright red color. It is a high-quality pigment and super antioxidant. It is a carotenoid with an o-hydroxyketone structure and its chemical name is 3,3-dihydroxy-4,4-di Keto-β,β'-carotene, pure astaxanthin is dark reddish brown crystals, melting point 224℃, insoluble in water, soluble in most organic solvents. Astaxanthin has super anti-oxidation and ability to scavenge free radicals. Clinical and zoological experiments show that astaxanthin has good anti-inflammatory, anti-tumor, anti-aging, skin protection, immune enhancement, prevention of cardiovascular diseases, It has many important biological functions such as maintaining eye health and improving animal reproduction. In addi...

Claims

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Application Information

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IPC IPC(8): C07C403/24
Inventor 李悦明管斌李元广徐建春夏修峦王道会
Owner QINGDAO KEHAI BIOLOGICAL
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