Method for extracting cephalotaxine from cephalotaxus hainanensis

A technology of harringtonine and crude torreya, applied in the field of harringtonine extraction, which can solve the problems of unfavorable industrial production, long consumption time and low efficiency

Inactive Publication Date: 2021-05-07
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The application provides a method for extracting harringtonine from Hainan crude torreya, which is used to solve the problem of extracting harringtonine from the existing harringtonine plants using organic solvents such as ethanol and chloroform, which are costly and expensive. It is conducive to the realization of industrial production, and column chromatography is often used for separation and purification. The technical problems of this method are large amount of solvent, long time consumption and low efficiency.

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  • Method for extracting cephalotaxine from cephalotaxus hainanensis
  • Method for extracting cephalotaxine from cephalotaxus hainanensis

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Embodiment 1

[0026] An embodiment of a method for extracting harringtonine from Hainan rough torreya provided by the present application, a method for extracting harringtonine from Hainan rough torreya, comprises the following steps:

[0027] Step 1. Crush the leaves of Torreya hainanensis and sieve for later use;

[0028] Step 2. Weigh the Hainan coarse torreya powder with a preset particle size and place it in a centrifuge tube, add an extraction solvent according to the preset material-to-liquid ratio, and then put it into an ultrasonic extraction device for ultrasonic extraction to obtain the first extraction solution, wherein, The extraction solvent is aqueous sulfuric acid;

[0029] Step 3. Put the first extraction solution into a centrifuge for centrifugation, pour out the supernatant after centrifugation, repeat the operation of adding extraction solvent, ultrasonic extraction, centrifugation, and pour out the supernatant after centrifugation for 1 to 4 times, and combine supernat...

Embodiment 2

[0035] A method for extracting harringtonine from Torreya hainanensis, comprising the following steps:

[0036] Step 1, after pulverizing the leaves of Torreya hainanensis, pass through a 10-mesh sieve;

[0037] Step 2. Weigh 0.2g Hainanese Torreya powder into a 15mL centrifuge tube, add 8mL sulfuric acid aqueous solution (pH=1) according to the ratio of solid to liquid at 1:40 for ultrasonic extraction, ultrasonic conditions: temperature is 28°C, time is 30min, the power is 600W, and the first extraction solution is obtained after the ultrasound is finished;

[0038] Step 3. Put the first extraction solution into a centrifuge device for centrifugation. Centrifugation conditions: speed 10000r / min, time 5min, pour out the supernatant after centrifugation, repeat adding extraction solvent, ultrasonic extraction, centrifugation, centrifugation end Finally, the supernatant was poured out for 4 times, and the supernatant obtained by each centrifugation was combined to obtain the s...

Embodiment 3

[0042] A method for extracting harringtonine from Torreya hainanensis, comprising the following steps:

[0043] Step 1, after pulverizing the leaves of Torreya hainanensis, pass through a 10-mesh sieve;

[0044] Step 2. Weigh 0.1g of Hainan Torreya hainanensis powder and place it in a 15mL centrifuge tube. Add 2mL of sulfuric acid aqueous solution (pH=1) for ultrasonic extraction according to the ratio of solid to liquid at 1:20. Ultrasonic conditions: temperature is 28°C, time is 10min, the power is 600W, and the first extraction solution is obtained after the end of ultrasound;

[0045] Step 3. Put the first extraction solution into a centrifuge device for centrifugation. Centrifugation conditions: speed 10000r / min, time 5min, pour out the supernatant after centrifugation, repeat adding extraction solvent, ultrasonic extraction, centrifugation, centrifugation end Finally, the supernatant was poured out once, and the supernatant obtained by each centrifugation was combined t...

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Abstract

The invention discloses a method for extracting cephalotaxine from cephalotaxus hainanensis, after an extraction solvent is added, cephalotaxus hainanensis powder is extracted by an ultrasonic extraction device to obtain a first extracting solution, and a sulfuric acid aqueous solution or a methanol solution is used as the extraction solvent, so that economy and environmental friendliness can be realized, and industrial production can be realized; the first extracting solution is centrifuged, then supernate is poured out, the operations of adding the extracting solvent, carrying out ultrasonic extraction, centrifuging and pouring out the supernate after centrifuging are repeatedly executed for 1-4 times, the supernate is combined to obtain a second extracting solution, and the second extracting solution is successfully separated and purified to obtain a cephalotaxine monomer by adopting a method for separating and purifying cephalotaxine through preparative high performance liquid chromatography. The extraction quality of the cephalotaxine can be improved through multiple extraction, the efficiency is high, and the technical problems that in the prior art, the cephalotaxine is extracted from cephalotaxus plants through organic solvents such as ethyl alcohol, the cost is high, industrial production is not facilitated, column chromatography is mostly adopted during separation and purification, the solvent dosage is large, and the efficiency is low are solved.

Description

technical field [0001] The present application relates to the technical field of harringtonine extraction methods, in particular to a method for extracting harringtonine from Hainan rough torreya. Background technique [0002] Torreya hainanensis is a tree of the genus Triceratophanaceae in the family Triaxaceae. The leaves, branches, seeds and roots of Torreya hainanensis contain 11 kinds of harringtonine alkaloids such as harringtonine and homoharringtonine. The harringtonine alkaloids have remarkable anti-tumor effects, and their content in plants is extremely low, and their natural production cannot meet the market demand, but most of them contain the same core skeleton——harringtonine. Therefore, extracting harringtonine from plants and then obtaining harringtonine alkaloids through a semi-synthetic method is expected to solve the resource supply problem of harringtonine alkaloids. Extracting harringtonine from the existing harringtonine plants all adopts organic solven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/20
CPCC07D491/20
Inventor 高非沈芸赵德庆赵曼娜王小慧罗海燕房一明王荣香
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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