The invention relates to an extraction method of
mangrove plant DNA, comprising the following steps: (1) taking a leaf to
grind, adding preheated 2% of CTAB extract to dissolve, and then adding 2 % of PVP by volume; (2) after water bath, adding a small quantity of RNase for heat preservation, then adding
chloroform-isoamylol mixed liquor, and carrying out high-speed
centrifugation at normal temperature; (3) putting supernate into a
centrifuge tube, adding the preheated 2% of CTAB extract, adding
chloroform-isoamylol mixed liquor, and mixing and carrying out high-speed
centrifugation at normal temperature; (4) taking supernate and then putting into the
centrifuge tube, adding NaCl solution and cold isopropanol, mixing, and putting and precipitating
DNA at low temperature; (5) carrying out high-speed
centrifugation at low temperature, abandoning the supernate, recovering the precipitate, washing by
ethanol, air-
drying, adding
TE buffer solution, and storing at low temperature. The invention can effectively remove
polysaccharide and
tannin produced in the
DNA extraction process, and the obtained
mangrove plant has high DNA content and high purity.