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SSR (Simple Sequence Repeat) core primer group and method thereof for identifying tea variety

A core primer and variety technology, applied in the biological field, can solve the problems of low efficiency, poor identification result accuracy, time-consuming and labor-intensive problems

Inactive Publication Date: 2014-08-27
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, tea tree varieties are usually identified based on morphological differences, but the same variety may show some trait differences under different site conditions, and some traits may also be similar among different varieties. get accurate conclusions
The specificity, consistency and stability (DUS) test of new tea tree varieties can accurately identify different tea tree varieties, but it needs to test 35 traits such as plants, shoots, leaves, etc., which is time-consuming and laborious.
At present, there are many problems in the identification of tea tree varieties, such as heavy workload, low efficiency and poor accuracy of identification results.

Method used

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  • SSR (Simple Sequence Repeat) core primer group and method thereof for identifying tea variety
  • SSR (Simple Sequence Repeat) core primer group and method thereof for identifying tea variety
  • SSR (Simple Sequence Repeat) core primer group and method thereof for identifying tea variety

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Embodiment 1

[0059] 1) Use and preparation of main reagents:

[0060] ① Genomic DNA extraction kit: a quick plant genome extraction kit produced by Beijing Tiangen Biochemical Technology Co., Ltd.;

[0061] ②Reagents required for PCR reaction: use rTaq enzyme produced by Dalian Bao Biological Engineering Co., Ltd., including 50 μL 5U / μL rTaq enzyme, 1 mL 10×Buffer, 800 μL dNTP Mixture and 1 mL Loading Buffer;

[0062] ③1L 5×TBE: 54g Tris, 27.5g boric acid, 20mL 0.5M EDTA (pH 8.0);

[0063] ④500mL 30% acrylamide: 145g acrylamide and 5g N, N-dimethylene bisacrylamide, add ddH 2 O is fixed to 500mL;

[0064] ⑤50mL 10% ammonium persulfate: 5g ammonium persulfate, add ddH 2 O is fixed to 50mL;

[0065] ⑥1L fixative: 100ml ethanol solution, 5mL glacial acetic acid, add distilled water to make up to 1L;

[0066] ⑦1L staining solution: 2g AgNO 3 , add distilled water to make up to 1L;

[0067] ⑧1L chromogenic solution: Prepared with distilled water, 15g NaOH solution and added 5mL 37% forma...

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Abstract

The invention relates to an SSR (Simple Sequence Repeat) core primer group and a method thereof for identifying a tea variety, and belongs to the technical field of biology. The method is characterized by comprising the following steps: (1) material selection; (2) genome DNA extraction; (3) PCR (Polymerase Chain Reaction) amplification of a target product; (4) electrophoretic separation of a PCR product; (5) silver staining development; (6) data analysis; and (7) result judgment. The SSR core primer group and the method thereof for identifying the tea variety, which are disclosed by the invention, have the characteristics of easiness and convenience for operation, accuracy in result, high repeatability, high identification capacity, low cost and the like and are suitable for identifying the tea variety.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to an SSR core primer set and a method for identifying tea tree varieties. Background technique [0002] Tea is an important economic crop in my country. By the end of 2010, there were 121 national-level approved varieties and more than 100 provincial-level approved varieties in my country. Realizing the effective identification of each variety will help protect variety rights and prevent counterfeit, shoddy or impure varieties from entering the market. At present, tea tree varieties are usually identified based on the morphological differences among them. However, the same variety may show some differences in some traits under different site conditions, and some traits may be similar among different varieties. get accurate conclusions. The specificity, uniformity, and stability (DUS) test of new tea tree varieties can accurately identify different tea tree varieties, but it needs to ...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 张成才成浩王丽鸳韦康吴立赟刘圆
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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