The invention provides a unicell based immunocyte
typing quantitative
analysis method. The method comprises that (1) a blood sample or a focus
tissue biopsy sample of a patient is collected; (2) an
antibody labeled with
metal elements is used to mark cells of the sample with multiple targets at the same time; (3) the marked
cell sample is detected by a
mass spectrum flow type detection
system; (4)on the basis of the obtained
mass spectrum flow type detection data, different immunocyte subgroups are clustered for analysis, and the expression level of the different subgroup cells in a tumor immune target is quantitatively analyzed; and (5) after
data processing and calculation of a dimension reducing
algorithm, a 2D point diagram, a SPADE graph or a thermograph are drafted, data is displayed in a visualized way, and an
informatics analysis model is constructed to cluster and analyze the different immunocyte subgroups. The rare immunocyte groups and
antigen with lower expression are detected sensitively by high-flux analysis detection, the immunization
typing analysis is more accurate, and the method has prospects of clinical application.