Rapid identification of powdery mildew resistance Lotus corniculatus genes utilizing comparative genomics

A powdery mildew resistant gene and lotus japonicus technology, applied in genetic engineering, plant gene improvement, application, etc., can solve the problems of difficult cloning and long time, and achieve the effect of shortening the breeding period, shortening the time, and improving the breeding efficiency

Inactive Publication Date: 2012-10-03
常熟市支塘镇新盛技术咨询服务有限公司
View PDF0 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the basic research of japonicus japonicus is not deep enough, it is not only time-consuming to use these methods but also it is difficult to clone these genes accurately

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rapid identification of powdery mildew resistance Lotus corniculatus genes utilizing comparative genomics
  • Rapid identification of powdery mildew resistance Lotus corniculatus genes utilizing comparative genomics
  • Rapid identification of powdery mildew resistance Lotus corniculatus genes utilizing comparative genomics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. The method can quickly identify the powdery mildew gene of japonicus japonicus. The specific implementation process is as follows:

[0032] 1) Collection and identification of MLO-type genes of japonicus japonicus

[0033] In order to obtain all the MLO-type gene family members of japonicus japonicus, we first constructed an HMM model using the MLO-type genes of Arabidopsis thaliana, and the MLO gene sequences of resistance to powdery mildew of tomato, pea, pepper, rose, and japonicus japonicus. Search for MLO-type genes in the genome sequence; secondly, use the published MLO gene sequences in different crops as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC,315947 TC325903, TC315944, TC315912, TC322...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to rapid identification of powdery mildew resistance Lotus corniculatus genes utilizing comparative genomics, relates to knowledge of subjects such as plant comparative genomics, genetics and bioinformatics, and belongs to the technical field of plant biotechnology. The rapid identification of powdery mildew resistance Lotus corniculatus genes utilizing the comparative genomics mainly includes the steps of firstly, downloading full genomic sequences of Lotus corniculatus and collecting MLO (mildew resistance locus o) genes; secondly, identifying the MLO genes; thirdly, identifying phylogenetic relationship of the MLO genes; and fourthly comparing the MLO powdery mildew resistance genes. By the rapid identification, mining cycle of the powdery mildew resistance genes is shortened, and the powdery mildew resistance genes can be identified quickly. Corresponding co-separation functional marks (SNP (single nucleotide polymorphism), Scar and the like) can be developed through candidate powdery mildew resistance genes identified, the rapid identification is also available for molecular marker-assisted selection of the powdery mildew resistance genes, and accuracy is high. The rapid identification can also be used with other molecular markers for resistance genes to create multiresistance breeding materials, and accordingly breeding period is shortened, breeding efficiency is improved, and basis for elaborating powdery mildew resistance Lotus corniculatus molecular mechanisms is laid.

Description

technical field [0001] The present invention uses methods such as plant comparative genomics, genetics, bioinformatics and candidate gene strategies to quickly identify the powdery mildew gene of japonicus japonicus by means of sequencing the whole genome sequence of japonicus japonicus, and mainly involves the whole genome sequence of japonicus japonicus Downloading, identification of candidate genes, gene comparison, clustering and other means to identify powdery mildew genes belong to the field of plant biotechnology science. Background technique [0002] japonicus ( Lotus corniculatus ), also known as five-leaf clover (four-leaf clover), bird's foot bean, and ox horn flower, is a perennial leguminous lotus root herb, native to the temperate zone of Eurasia, in my country's Hebei, Yunnan, Guizhou, Sichuan, Gansu, etc. It is distributed all over the land, japonicus japonicus is a forage plant widely used in orchard grass, green manure, and grassland improvement, and it is ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12Q1/68A01H5/00
Inventor 钱孝英俞锞刘云飞
Owner 常熟市支塘镇新盛技术咨询服务有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap