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Bottle gourd anti-anthracnose gene fragments or gene markers, and applications thereof

A technology of gene fragments and gene markers, applied in various fields, can solve problems affecting the growth and development of gourd plants, economic losses, etc., and achieve the effects of improving breeding efficiency, shortening the breeding period, and shortening the time

Inactive Publication Date: 2015-05-13
CHANGSHU CITY BEIBANG TOWN BEIGANG VEGETABLE SPECIALIZED COOP
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Anthracnose seriously affects the normal growth and development process of gourd plants, bringing great economic losses to production

Method used

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  • Bottle gourd anti-anthracnose gene fragments or gene markers, and applications thereof
  • Bottle gourd anti-anthracnose gene fragments or gene markers, and applications thereof
  • Bottle gourd anti-anthracnose gene fragments or gene markers, and applications thereof

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Embodiment Construction

[0017] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. This method can quickly identify the anthracnose resistance gene of gourd. The specific implementation process is as follows:

[0018] 1. DNA extraction: Take an appropriate amount of young gourd leaves, dry them with silica gel, grind them into powder, and extract the total genomic DNA with the CTAB method. The extracted DNA was detected by an ultraviolet spectrophotometer, and samples with an OD260 / OD280 value of 1.8-2.0 were selected and stored at -20°C for future use.

[0019] 2. Amplification and cloning of the homologous fragment of the disease resistance gene: according to the amino acid sequence GG(L / I / V / M / S)GKTT and G(L / N) of the conserved region of the known NBS-LRR type disease resistance gene ) PL(A / T / S)(L / V), designed a pair of degenerate primers: NBS15'-GKTT GGIGGIWSIGGIAARA...

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Abstract

The present invention relates to bottle gourd anti-anthracnose gene fragments or gene markers, and applications thereof. According to the present invention, a disease resistance gene homologous sequence method is adopted, two bottle gourd anti-anthracnose gene fragments or gene markers are rapidly separated, sequencing is performed, and the nucleotide length of the gene fragments or gene markers are respectively 495 bp and 510 bp; the bottle gourd anti-anthracnose gene finding period is effectively shortened so as to easily achieve rapid anthracnose gene identification; the corresponding co-segregation function marker (SNP, SCAR and the like) is developed through the identified anthracnose gene, the bottle gourd anti-anthracnose gene fragments or gene markers can further be rapidly used for the molecule marker-assisted selection of the anti-anthracnose gene, and the accuracy is high; the bottle gourd anti-anthracnose gene fragments or gene markers can be combined with other disease resistance gene molecule markers so as to create multi-resistance breeding materials, shorten the breeding period, and improve the breeding efficiency; and the foundation is established for exposition of the bottle gourd anti-anthracnose gene molecule mechanism.

Description

technical field [0001] The present invention uses the homologous sequence method of disease-resistant genes to clone gourd anti-anthracnose gene fragments or convert them into molecular markers, etc., and mainly relates to gourd conventional hybrid breeding, molecular marker-assisted breeding, positioning and anthracnose-resistant genes. Molecular mapping, cloning of anthracnose-resistant genes, and analysis of the regulatory mechanism of disease-resistant genes belong to the field of plant biotechnology science. Background technique [0002] Anthracnose is one of the most important diseases in gourd production. When the seedlings are infected, slightly sunken round or oval yellow-brown lesions are produced on the edge of the cotyledon, with obvious edges and rough diseased parts. When the leaves are infected at the adult plant stage, water-soaked circular spots are initially produced, which are slightly sunken, and after expanding, the diameter is about 2 to 3 cm, and grad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 钱孝英钱春桃
Owner CHANGSHU CITY BEIBANG TOWN BEIGANG VEGETABLE SPECIALIZED COOP
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