105 results about "Multi resistance" patented technology

A resistive random access memory including two electrode layers and a multi-resistance layer mounted between the two electrode layers. The multi-resistance layer consists essentially of insulating material with oxygen and lithium ions. The number of resistance states of a memory element can be increased by the resistive random access memory to increase the integration density of a memory module having a plurality of memory elements.

The invention relates to the field of fertilizers, and discloses a special multi-resistance drug fertilizer for super rice and a preparation method thereof. The drug fertilizer disclosed by the invention comprises an insecticide, a bactericide and a fertilizer. In the drug fertilizer disclosed by the invention, the insecticide and bactericide are organically combined according to the rice nutrition characteristics, the soil fertility conditions and the rice pest and disease damage occurrence characteristics; in cooperation with certain amount of nutrient element fertilizer, the fertilizer drugs are combined and interact for synergism; nutrient elements are effectively supplemented while enhancing the rice resistance to pest and disease damage, the needs for nutrients of the rice are better met, the insecticidal and disease-resistant effects can be realized by once application of the fertilizer, and the manual work frequency is effectively reduced; moreover, the fertilizer is low-toxicity and safe to the environment and human and livestock, and is especially applicable to the super rice variety with high nutritional requirements and susceptible to pests and diseases. Experiments indicate that the drug fertilizer disclosed by the invention has the advantages of good insecticidal and disease-resistant effects, comprehensive nutrition, balanced and lasting nutrient supply and long lasting period; meanwhile, the labor intensity and cost in cultivation are reduced, the rural income is increased, and the labor is saved while the yield is increased; the fertilizer is energy-saving and efficient and safe and environment-friendly.

The invention provides a double-mode type active power filter circuit with an adjustable bandwidth. The filter circuit can be configured to be at two modes of a complex band-pass filter and a low-pass filter which are respectively used for a receiving chain and an emitting chain in a wireless receiver chip. The filter circuit comprises a first third-order low-pass filter, a second third-order low-pass filter, a multi-resistance network, a first extra input end, a second extra input end, a first first-order passive RC (Remote Control) filter and a second first-order passive RC filter. The filter circuit has the advantages that the double-mode type active power filter circuit can be controlled through an internal switch and can be configured to be at the two work modes, so that the area of the chip is saved and the cost of a system is reduced.

The invention discloses a memristor-based nonvolatile multivalued logic device and an operating method thereof. The method provides an operating method for negation and dextrorotation of three-valued logic and multivalued T gate logic, which realizes an exhaustive set of multivalued logic, such as three-valued logic based on the multi-resistance state transition characteristic of a memristor. All the logic operation results are stored in the device in a nonvolatile resistance state, such that a logic operation function and a data storage function are realized simultaneously in the device, i.e., storage and calculation are fused; therefore, a device basis is laid for going beyond the limitation from an information device Moore's law and breaking through the Von Neumann bottleneck in a computer architecture. The memristor-based multivalued logic device disclosed by the invention can be applied to a novel solid-state memory, a logic-arithmetic unit, a programmable gate array, an on-chip system and other fields as a basic unit, and provides a new path for boosting novel computer architecture.

The present invention relates to a multi-stopband ultra-wideband antenna realized through patch gap etching, which consists of a dielectric substrate (1) and a metal clad layer(2) on the dielectric substrate (1). A feeder line part adopts a coplanar waveguide form, and consists of a signal line (3), gaps (4) and metal areas (5) which are symmetrically distributed on two sides of the signal line (3); a radiation unit (6) adopts a disk form; an extended end of the signal line (3), which is slightly longer than the metal areas (5) on two sides, is connected with the radiation unit (6); the antenna has ultra-wideband characteristic, which can meet the requirements of ultra-wideband communication systems; meanwhile, the antenna can generate stopbands at three frequencies, thereby having the performance of resisting multiple frequency point interference. As the frequency of the antenna stopband can be freely regulated by changing etching gap length, the antenna has stopband adjustability, which can satisfy the application in some special interference frequency occasions. In addition, the increase of etching gaps can realize more stopband characteristics.

The invention relates to a method for preparing a multi-resistance organic fertilizer of tea seed cake and belongs to the technical field of ecological organic fertilizer. The method is characterized in that the formula comprises the following raw materials in weight percentage: 20 to 25 percent of camellia seed cake, 8 to 12 percent of beer residue, 3 to 8 percent of citric acid multibacteria, 3 to 8 percent of potassium sulfate, 9 to 15 percent of calcium magnesium phosphate fertilizer, 0.1 percent of active biological fermenting agent, 3 to 8 percent of tobacco rod, 30 to 40 percent of carrier and 3 to 10 percent of water. The method has long and stable fertilizer effect, can improve soil, is biological, environment-friendly and relatively time-saving and labor-saving in fostering, collecting and composting the fertilizer, has good lodging resistance and plant disease and insect pest resistance and high commercialization degree, can be widely applied to the production of a harmless green farm product, and is in particular suitable for the production of an AA-grade and an A-grade green healthy farm products.

The invention provides a grape multi-resistance rootstock hard branch grafting nutrition bag seedling raising method in arid desert areas. The grape multi-resistance rootstock hard branch grafting nutrition bag seedling raising method in the arid desert areas adopts rootstocks which are selected from 5BB and / or S 04, and adopts following steps of gathering of scions, selection of rootstocks, pavement of double layers of electric heating hotbeds, grafting of the rootstocks and the scions, placement of cuttages on the electric heating hotbeds, placement of the cuttages in nutrition bags, and management after cutting of the rootstocks. A plurality of selection tests of rootstock varieties come to a discovery that after rootstocks S04 and rootstocks 5BB are grafted with local cladus, nursery stocks are good in resistances, low in infection rate of diseases and insect pests, strong in saline-alkaline resistance, high in survival rate, and rather good in local adaptability. Greenhouses are further used for carrying out large-scale fast breeding of grape hard branch grafting seedlings, breeding stage is short, and grafting, breeding and planting of the seedlings can be achieved in the same year. The grape multi-resistance rootstock hard branch grafting nutrition bag seedling raising method in the arid desert areas is particularly suitable for arid and saline-alkaline desertification areas, simple and practical in technology, and easy to popularize.

The present invention relates to a multi-stopband ultra-wideband antenna based on split ring resonators and patch etching gaps, comprising a disk antenna and a split ring resonator coupling micro-strip structure. A lower surface metal clad layer (2) is the ground of a feeder part. An upper surface metal clad layer (3) comprises an antenna radiation unit (4), a micro-strip feeder (5), a trapezoidal impedance transformation line (6) and a split ring resonator (8), wherein, the antenna radiation unit (4) adopts the disk antenna on which split ring shape gaps (7) with the same circle center are arranged; the back of the disk antenna has no metal area; the micro-strip feeder (5) adopts a micro-strip line form; the micro-strip feeder (5) and the antenna radiation unit (4) are in impedance matching connection through the trapezoidal impedance transformation line (6); the split ring resonator (8) comprises two pairs of resonators, namely a first resonator (9) and a second resonator (10), which are symmetrically distributed on two sides of the feeder; each resonator is formed by an inner split ring and an outer split ring, and the opening direction of the inner ring is far away from the feeder, while he opening direction of the outer ring is close to the feeder.

The invention provides a breeding method for a multi-resistance flue-cured tobacco hybrid. The breeding method for the multi-resistance flue-cured tobacco hybrid comprises the steps of firstly using MSG28 as a female parent, using high-quality, widely applicable and blackleg-resistant varieties which are sensitive to TMV and weather fleck and are used for production as recurrent parents, performing eight generations of backcrossing to breed male sterile lines, using fertile materials which are resistant to blackleg and highly resistant to TMV and weather fleck as original resources, performing six generations of selfing purification to breed selfing pure lines with common or better curing characteristics, then using the bred male sterile lines as female parents and the selfing pure lines as male parents to breed hybrid combinations, respectively evaluating resistance to different diseases and economic performance by using land parcels or areas seriously attacked by different diseases and normal land parcels in producing areas, and finally performing 2-3 years of multi-point tests for quality and adaptability evaluation to the hybrid combinations which are simultaneously resistant to different diseases and have good economic performance. By adopting the method, the aggregation of multiple characters such as high quality, simultaneous resistance to foliage diseases and root and stalk diseases and good adaptability can be realized, the breeding efficiency is improved.

The invention relates to the technical field of semiconductor manufacturing, and discloses a multi-resistance-state resistance random access memory. The memory comprises a top electrode, a resistance change layer and a bottom electrode from top to bottom, wherein the resistance change layer is an oxide. The invention also provides a method for implementing multiple resistance states by utilizing the memory. The multi-resistance-state resistance random access memory provided by the invention is a device in an MIM (metal-insulator-metal) structure based on a SiO2 material. Under an appropriate operation method, the device can generate multi-stage resistance states, multi-bit memory can be realized in a unit, and the memory density of the memory can be improved; and the material based on SiO2 is adopted for the resistance change layer of the multi-resistance-state resistance random access memory, the memory and the method provided by the invention have the advantages of simple technology and good processing compatibility with the CMOS (complementary metal-oxide-semiconductor transistor) technology.

The invention discloses a breeding method for black and white sweet glutinous hybrid maize. The breeding method for black and white sweet glutinous hybrid maize comprises the steps of selecting ultra-sweet maize, glutinous maize, a common high yield multi-resistance maize-inbred line and the like from different regions to be equivalently mixed, openly pollinating the maize, establishing a basic group A, selecting black glutinous maize germplasm from different regions to be equivalently mixed, openly pollinating the maize, and establishing a basic group B; then carrying out recurrent selection to the properties of seeds of the maize to respectively form a white sweet glutinous maize recurrent selection group A and a black sweet glutinous maize recurrent selection group B, obtaining a serious inbred line through multi-generation inbreeding, and screening a white sweet glutinous maize inbred line 4587 and a black sweet glutinous maize inbred line 3303 through combination ability measurement and cross combination quality identification to obtain 4587 X black 3303 hybrid. The breeding method can ensure both the glutinous and sweet genes of a parent inbred line and the hybrid of the parent inbred line, and also ensure the appearance quality, the favorable edible quality, the stability and the consistency of the hybrid. Moreover, the seeds of the maize are black and white.

The invention discloses a multi-system additive operation circuit based on memristors. The multi-system additive operation circuit comprises the first memristor, the second memristor, the third memristor, a first right-handed rotation logic door, a second right-handed rotation logic door, a first voltage converter and a second voltage converter. The input end of the first right-handed rotation logic door serves as the input end of the multi-system additive operation circuit. The first right-handed rotation logic door, the first voltage converter, the second right-handed rotation logic door and the second voltage converter are sequentially connected. One end of the first memriser, one end of the second memriser and one end of the third memriser are connected to serve as the output end of the multi-system additive operation circuit. The other end of the first memriser is connected to the input end of the first right-handed rotation logic door, the other end of the second memriser is connected to the connecting ends of the first voltage converter and the second right-handed rotation logic door, and the other end of the third memriser is connected to the output end of the second voltage converter. The multi-system additive operation circuit can achieve multi-valued logic operation based on the characters of multi-resistance state transition and nonvolatile change of the memrisers.

The invention discloses a method for breeding and seed production of superior multi-resistance high-yield two-line hybrid rice. According to the method, superior rice-blast-resistant high combining ability photo-thermo sensitive genic male sterile line-guangzhan 63S-4 is taken as a female parent to hybridize with a superior bacterial blight-resistant strong and excellent restorer line-zhenhui 832, thus obtaining the two-line hybrid rice-liangyou 832 with high quality, high rice blast and bacterial blight resistance and high yield. The rice cultivated by the method has the advantages of good comprehensive yielding ability, moderate maturation period, high quality, good commodification and strong stress resistance.

The invention discloses a breeding method of a novel strain of a high-quality, high-yield, multi-resistance and high-combining ability corn selfing line. The breeding method comprises that through selfing of optimal plants of a corn line Swan(late)C4, the novel strain Guizhao 18421 of the high-quality, high-yield, multi-resistance and high-combining ability corn selfing line is obtained. The Swan(late)C4 as a base breeding material has high resistance, good appearance quality and good fecundity. Through circulation breeding and high-density selective stress, the Guizhao 18421 has excellent properties such as good quality, high yield, multi-resistance and high-combining ability and can be used as a base material for production of an excellent corn hybrid and improvement on corn seed quality.

The invention relates to a composite microbial fertilizer capable of preventing the vegetable root-knot nematode disease and a preparation method thereof, and belongs to the field of fertilizer production. The composite fertilizer is produced by the following steps: adding carbon containing plant powder and cereal protein powder into a decomposed organic fertilizer to prepare an organic substance, adding functional bacteria into the organic substance, and fully and evenly mixing to obtain the novel multi-resistance composite fertilizer. The functional bacteria can breed massively around the roots of vegetables and crops to form circular protective covers, thus the diseases caused by root-knot nematode are prevented effectively, a good ecological environment is created for the growth of vegetables, at the same time, the soil environment is improved, the soil hardening is avoided, the ventilation and oxygen content of soil are improved, the product quality and output are increased, and the fertilizer has the functions of pesticide and fertilizer. The organic substance content of the composite microbial fertilizer is not less than 50%, and the beneficial bacterium content is 6*10<9> CFU / g. The composite microbial fertilizer is easy to use, the fertilizing effect is high, the fertilizer only needs one application, labor is saved, and the environment is protected.

The invention provides a low-power-consumption magnetic multi-resistance-state memory cell. The low-power-consumption magnetic multi-resistance-state memory cell successively comprises an anti-ferromagnetic strip film, a first ferromagnetic metal, a first oxide, a second ferromagnetic metal, a first synthetic anti-ferromagnetic layer and a first electrode from bottom to top. The thickness of the anti-ferromagnetic strip film is 0-20 nm. The thickness of the first ferromagnetic metal is 0-3 nm. The thickness of the first oxide is 0-2 nm. The thickness of the second ferromagnetic metal is 0-3 nm. The thickness of the first synthetic anti-ferromagnetic layer is 0-20 nm. The thickness of the first electrode is 10-200 nm. The two ends of the anti-ferromagnetic strip film are respectively plated with a second electrode and a third electrode. Five layers of materials arranged above the anti-ferromagnetic strip film form material magnetic tunnel junctions. According to the technical scheme of the invention, the multi-resistance-state storage can be realized. The low-power-consumption magnetic multi-resistance-state memory cell can be applied not only to the fields of single-bit memory and logic operation, but also the fields of brain-like calculation and the like. Data are written by adopting a unidirectional current, so that the design of memories and logic circuits is simplified. The integration level of circuits is improved and the power consumption of the memory cell is reduced. The process complexity and the manufacturing cost are reduced. Moreover, data are written by using different branches, so that the write operations of different data are independently optimized and designed.

The invention relates to a bit structure of a multi-state magnetic memory. The bit structure of the memory comprises a multi-state magnetic tunnel junction memory unit array and a switch circuit composed of a word line, a bit line and a switching device. The bit structure of the multi-state magnetic memory has the advantages of fast reading speed, unlimited number of erasure, low energy consumption and radiation resistance belonging to the magnetic random access memory (MRAM). The multi-state magnetic memory realizes reprogramming only under magnetic field aid, has high safety and can be used as an ideal high-safety multi-programming memory. The multi-state magnetic tunnel junction memory unit is in a multi-resistance state and each memory unit can record 3 or 4 bits of information so that the data storage density is effectively improved and the chip area is reduced. The memory bit can be used for microprocessors and digital circuits with high requirements on characteristics such as high safety, fast reading speed, low power consumption and resistance to radiation and harsh environments.

The invention belongs to the technical field of corn breeding, and particularly relates to a breeding method for an inbred-line new strain of high-yield multi-resistance high combining ability corn. According to the breeding method, the inbred-line new strain of the high-yield multi-resistance high combining ability corn is bred through selfing of optimal plants of a corn strain PS group. The breeding method has the advantages that the PS group serving as a basic breeding material has high resistance, high appearance quality and high fecundity, and cyclic breeding and high-density selection stress strategies are adopted, so that the obtained SP221 integrates excellent traits such as high yield with multi-resistance and high combining ability, and can be applied to preparation of good cornhydrids and served as a basis material for corn variety improvement.

The present invention relates to a multi-stopband ultra-wideband antenna realized through split ring resonator coupling feeders, comprising a disk antenna and a split ring resonator coupling micro-strip feeder structure. The present invention is realized in a microwave dielectric substrate through printed circuit board technique. The split ring resonator coupling micro-strip feeder structure can generate stopband at a specific frequency point. A plurality of stopbands are generated by appending a plurality of split ring resonators in different sizes, so as to form a single-chip antenna feeder structure with multi-stopband characteristic. The multi-stopband ultra-wideband antenna realized through split ring resonator coupling feeders not only has ultra-wideband characteristic, but also has good inhibiting effect to interfering signals at a plurality of frequency points. The antenna is simple in structure and small in volume, meets the requirements of planar circuit integration, and has the advantages of low cost, batch production convenience, etc. Therefore, the antenna is suitable for various anti-interference ultra-wideband application occasions.

The invention discloses a breeding method of high-yield multi-resistance non-glutinous rice suitable for light simplified cultivation, which belongs to a breeding method of plant seeds. The method comprises the steps of generation segregation through early sowing at an early stage, screening through late sowing, high-density direct sowing, low-nitrogenous-fertilizer-level planting, direct sowing and mixed harvesting to obtain seeds which satisfy requirements. The rice bred through the method has the characteristics that the rice is suitable for light simplified cultivation, is high-yield and is multi-resistant. Under the transplanting condition of individual plants, the growth situation is good, the self-regulating capability is strong, the stem is robust, the rice is fertilizer-resistant and anti-falling, the rice can be directly planted in the early ten days and middle ten days of June, the spike length is generally and approximately 18cm, the number of productive spikes per mu can reach more than 200 thousand, the total grain number per spike is 130-140, the fruiting rate is above 90 percent, the weight per thousand grains is 27-28g, the rice grains are full, the commodity value is good and the processing quality is good.

The invention provides high-yield coenzyme Q10 rhodobacter sphaeroides and mutation breeding and application thereof, and relates to the field of microbial mutation breeding. Strains screened by spacemutagenesis are used as starting bacteria and subjected to plasma mutagenesis at normal pressure and room temperature. The high-yield coenzyme Q10 rhodobacter sphaeroides are obtained after multi-resistance screening, high-throughput screening and step-by-step amplification verification, and are deposited in China General Microbiological Culture Collection Center with the deposit number of CGMCCNo. 16625. The average titer of a mutant strain of the rhodobacter sphaeroides can reach 2694[mu] / mL according to 20m<3> fermentation production verification, and the yield of the coenzyme Q10 is significantly improved. In addition, the strain has quadruple resistance and broad application prospects.

The invention provides a graded porous oxide memristor shaped like a melting hole, and the basic structure of the memristor sequentially comprises a bottom electrode layer, oxide layers and a top electrode layer from bottom to top. The memristor is characterized in that the oxide layers serve as resistive function layers and comprise a first oxide layer and a second oxide layer, and the second oxide layer is close to one side of the bottom electrode layer; the second oxide layer is of a porous structure and is of a similar-melting-hole graded porous structure, wherein the porous channels of the porous structure are communicated with one another, and the transportation of metal ions can be regulated and controlled under the action of an electric field; when the external electric field is removed, metal ions can be stored. The oxide memristor has stable, multi-resistance-state and low-energy-consumption memristor characteristics, a new path is provided for exploration of a memristor mechanism, and the electrical performance of the memristor can be improved; the memristor has good characteristics in the aspect of artificial synapse simulation, and a great application prospect is provided for neuromorphic calculation.

The invention relates to a multi-resistance eurytopic selection mixed wheat breeding method, and belongs to the field of crop breeding. According to the method, plants are selected from a wheat hybridF2 generation, the selected plants are subjected to seed testing, then single ears are threshed and mixed, and an F3 generation is sown; the F3 generation continues to be subjected to plant selection, the selected plants are subjected to seed testing, then single ears are threshed and mixed, and an F4 generation is sown; the F4 generation is subjected to plant selection, seed testing and single-plant threshing, mixing is not carried out, and a plant strain starts to be identified; if the plant strain integrates many objective traits and trait expressions between the plants are consistent, a key strain can be determined, a plot identification test is improved, and 3-5 plants are reserved for purity preservation; if the traits of the plant strain are separated, single plants are selected inthe plant strain, and plant strain identification is carried out in the next year until a stable generation is obtained; finally, multi-point product comparison and identification are carried out onthe stable generation. According to the method, excellent genes can be well retained during seed selection of hybrid generations, excellent single plants are selected at the early stage, adverse individuals are eliminated, the breeding efficiency is improved, and the breeding time is shortened.

The invention relates to a structure for expanding the number of resistance states of a memristor and a related method, which can realize the structure for expanding the number of resistance states ina memristor cross array and comprise cross array units, resistance state expansion function line groups, input line groups, output line groups and unit connection line groups, Wherein the resistancestate expansion function line group passes through a plurality of resistance state expansion function lines of which the tail ends are first gating devices; Each two adjacent transverse lines or longitudinal lines in the cross array unit are connected, each first gating device enables a signal path of a resistance state expansion function line to be connected or disconnected according to signals of a control end, memristors connected to the transverse lines or the longitudinal lines receiving input signals are connected in parallel, and resistance state expansion is achieved. The invention also discloses a method for realizing the expansion of the number of resistance states in the memristor cross array and a method for writing the resistance values of the memristors in the cross array. The method has the advantages that power failure is not prone to losing, and the requirement for the multi-resistance state in mass weighted summation of the neural network is met.

The invention discloses a convergent cross breeding method for multi-resistance high-quality orange heart baby cabbage for spring and autumn. In order to solve problems, the method comprises the following steps of: selectively breeding a high-stalk encircling resistance source 322; selectively breeding an encircling orange heart material 02-693; selectively breeding a premature orange heart self-bred line 08P238; hybridizing a TuMV resistance source B75 and Fushan wrapped cabbage head, and then performing multi-generation inbred to obtain a property modified encircling resistance source 95; selectively breeding a bolting-resistant material 538; selectively breeding an extremely early orange heart strain 304; selectively breeding an orange heart baby cabbage self-bred line X08-219; performing coordinating test on the premature orange heart self-bred line 08P238 and the orange heart baby cabbage self-bred line X08-219; transforming the premature orange heart self-bred line 08P238 into a sterile line to obtain an orange heart male sterile line 08P238A; and hybridizing the orange heart baby cabbage self-bred line X08-219 and the orange heart male sterile line 08P238A to obtain an F1 crossbreed. The orange heart baby cabbage selectively bred by the method for spring and autumn is extremely early, has high virus disease resistance and orange color, and is suitable to be cultivated in spring and autumn.

The invention relates to a high-yield and multi-resistant method of hybrid seed production for corn variety, belonging to the field of taking advantages of corn hybrid seeds. The method comprises seed selection of inbred lines 216 and 86 by using inbred line 216 as female parent and inbred line 86 as male parent or by using inbred line 86 as female parent and inbred line 216 as male parent to prepare hybrid seed Bidan-13 through multi-generations self-pollination purification, and provides corresponding seed production technique and high-yield culture technique. The prepared Bidan-13 has the characteristics of high yield, good quality, multi-resistance, broad adaptability, and the like; with the average yield per mu of 585.12kg, the corn is averagely increased by 15.37% compared with Xinghuangdan-892, and is an ideal variety for current corn production.

The invention provides a planting method for multi-resistance and high-yield capsicum. The planting method comprises the following steps: (1) variety selection; (2) seedbed selection and fertility betterment; (3) seed soaking and seed disinfection; (4) seeding; (5) seedling management; (6) field selection and fertility betterment; (7) soil preparation, bedding and transplanting; (8) field management; (9) disease and pest control; (10) harvesting. The planting method is simple and easy to realize, is suitable for field cultivation of capsicum, is characterized by low investment cost, high yield and low disease and pest occurrence rate, and is conducive to improvement of quality of products and market competitiveness. The technique guarantees comprehensive application of green prevention and control measures for diseases, pests and weeds in all stages of seed selection, seedling culture and planting. A soil conditioner is added when base fertilizer is applied, so that soil quality is improved and breeding of diseases and pests is reduced; use of chemical pesticide is avoided and reduced to the maximum extent and quality safety of products is reliably guaranteed.

The invention relates to rapid identification of a pear powdery mildew resistance gene, and relates to the subject knowledge of plant comparative genomics, genetics and bioinformatics, belonging to the field of science of biotechnology. The rapid identification of the pear powdery mildew resistance gene comprises the following main steps of: 1) downloading of a complete genome sequence of a pear and collection of an MLO gene; 2) identification of the MLO gene; 3) MLO gene phylogenetic relationship; and 4) comparison of an MLO powdery mildew gene. The rapid identification of the pear powdery mildew resistance gene, provided by the invention, has the advantages of effectively shortening the pear powdery mildew gene mining period, and being favorable for the rapid identification of the powdery mildew gene; corresponding co-segregation functional markers (SNP, SCAR and the like) can be developed through the identification of candidate powdery mildew genes, and the co-segregation functional markers can be rapidly used for the molecular marker-assisted selection of the powdery mildew resistance gene, so that the accuracy is high; by combining other powdery mildew resistance gene molecular markers, a multi-resistance breeding material can be created, the breeding period can be shortened, and the breeding efficiency can be improved; a foundation is laid for explaining a pear powdery mildew resistance molecular mechanism.

The invention provides a breeding method of multi-resistance flue-cured tobacco three-way cross varieties. The breeding method comprises the steps: with a conventional germplasm material A or a male sterility line B having good quality and comprehensive economic characters and also having resistance to brown spot, black shank and bacterial wilt as a female parent, and with a conventional germplasm material C having general quality and comprehensive economic characters and also having resistance to TMV, climate spot, black shank and bacterial wilt as a male parent, assembling to obtain a single-cross combination A / C or B / C; and then with a conventional variety D having excellent quality, wide adaptability, good comprehensive economic characters and resistance to black shank and bacterial wilt as a male parent, with the single-cross combination B / C as a female parent, preparing the three-way cross variety B / C / / D, or with a male sterile line E of the conventional variety D as a female parent, and with the single-cross combination A / C as a male parent, preparing the three-way cross variety E / / A / C. The method can effectively integrate excellent genes of different parent materials, and the bred three-way cross varieties have the characteristics of good quality, wide adaptability, good comprehensive economic characters, and resistance to a variety of root and stem diseases and foliar diseases.

The invention provides rapid identification of soybean anti-powdery mildew gene, relates to knowledge in the disciplines of plant comparative genomics, genetics and bioinformatics, and belongs to the scientific field of plant biotechnology. The invention includes the steps of: 1) downloading the soybean whole genome sequence and acquiring MLO type gene; 2) identifying the MLO genotype; 3) identifying MLO gene phylogenetic relationship; and 4) comparing MLO-type powdery mildew gene. The invention effectively shortens the gene mining cycle for soybean powdery mildew, and is conducive to the rapid identification of anti-powdery mildew gene; corresponding coseparation functional markers (SNP, scar, etc.) are developed through the identified candidate powdery mildew genes, and the identified candidate powdery mildew genes can also be used for fast molecular marker assisted selection of anti-powdery mildew genes and have high accuracy; and combined with other resistant gene molecular markers, multi-resistance breeding material can be created, so as to shorten the breeding period and improve breeding efficiency. Therefore, the invention lays foundation for the explanation of powdery mildew resistant molecular mechanism of soybean.