Breeding method of multi-resistance flue-cured tobacco three-way cross varieties

A three-cross, resistance technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of poor quality and adaptability, can not simultaneously resist foliar diseases and rhizome diseases, etc. The effect of breeding efficiency, solving poor quality and wide adaptability

Inactive Publication Date: 2016-01-06
HUBEI TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, my country's tobacco breeding units have bred a number of flue-cured tobacco hybrids, such as Yunyan 201, Jiyan 9, Liaoyan 16, etc., but these hybrids are a

Method used

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  • Breeding method of multi-resistance flue-cured tobacco three-way cross varieties
  • Breeding method of multi-resistance flue-cured tobacco three-way cross varieties
  • Breeding method of multi-resistance flue-cured tobacco three-way cross varieties

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Obtainment of inbred pure lines of flue-cured tobacco parent materials K730, RG11, G80, and Coker176

[0024] The Hubei Academy of Tobacco Science utilizes southern propagation and local breeding bases, and uses the flue-cured tobacco varieties K730, RG11 and G80 introduced from the United States as original resources. "Bacteria wilt" is the breeding goal, and the self-purification of selected strains is carried out alternately in the southern and northern environments with large climate differences. After 7 generations of self-purification, genetically stable inbred lines of K730, RG11 and G80 are obtained. ;Using the flue-cured tobacco variety Coker176 imported from the United States as the original resource, according to the breeding goal of "good quality and comprehensive economic traits, and resistance to TMV, climate spot, black shank and bacterial wilt" as the breeding goal, it has been continuously cultivated in southern China with large climate differ...

Embodiment 2

[0025] Example 2: Acquisition of flue-cured tobacco parent materials MSK730, MSRG11, MSG80, and MSK326

[0026]Hubei Institute of Tobacco Science used the flue-cured tobacco male sterile line MSG28 as the female parent, and used the K730 inbred line, RG11 inbred line, G80 inbred line obtained in Example 1 and the conventional variety K326 used in production. As the male parent, crossbreed to obtain the male sterile F1 generation MSG28 / K730 inbred line, MSG28 / RG11 inbred line, MSG28 / G80 inbred line and MSG28 / K326, and then use the southern breeding and local breeding bases to The male parent of the male sterile F1 generation is a recurrent parent, and the corresponding male sterile F1 generation is continuously selected and backcrossed alternately in the southern and northern environments with large climate differences. After 8 generations of backcrossing, the male sterile of flue-cured tobacco is obtained Lines MSK730, MSRG11, MSG80 and MSK326. The quality, comprehensive econ...

Embodiment 3

[0027] Embodiment three: the preparation of three crosses

[0028] (1) Preparation of three-cross hybrid HB129

[0029] In the normal tobacco growing season of the breeding base of Hubei Tobacco Research Institute, the K730 inbred pure line and the Coker176 inbred pure line obtained in Example 1 were used as the female parent and the male parent respectively to prepare a fertile single-cross combination K730 inbred pure line / Coker176 inbred pure line. In the same year, in the Southern Breeding Base, the male sterile line MSK326 obtained in Example 2 was used as the female parent, and the above-mentioned fertile single-cross combination K730 self-bred pure line / Coker176 self-bred pure line was used as the male parent to form a three-cross hybrid MSK326 / / K730 inbred line / Coker176 inbred line, numbered HB129.

[0030] (2) Preparation of three-cross hybrid HB165

[0031] In the normal tobacco growing season of the breeding base of Hubei Tobacco Research Institute, the male ste...

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Abstract

The invention provides a breeding method of multi-resistance flue-cured tobacco three-way cross varieties. The breeding method comprises the steps: with a conventional germplasm material A or a male sterility line B having good quality and comprehensive economic characters and also having resistance to brown spot, black shank and bacterial wilt as a female parent, and with a conventional germplasm material C having general quality and comprehensive economic characters and also having resistance to TMV, climate spot, black shank and bacterial wilt as a male parent, assembling to obtain a single-cross combination A/C or B/C; and then with a conventional variety D having excellent quality, wide adaptability, good comprehensive economic characters and resistance to black shank and bacterial wilt as a male parent, with the single-cross combination B/C as a female parent, preparing the three-way cross variety B/C//D, or with a male sterile line E of the conventional variety D as a female parent, and with the single-cross combination A/C as a male parent, preparing the three-way cross variety E//A/C. The method can effectively integrate excellent genes of different parent materials, and the bred three-way cross varieties have the characteristics of good quality, wide adaptability, good comprehensive economic characters, and resistance to a variety of root and stem diseases and foliar diseases.

Description

technical field [0001] The invention belongs to the technical field of tobacco breeding, and in particular relates to a multi-resistance flue-cured tobacco three-cross breeding method. Background technique [0002] Flue-cured tobacco is the main raw material crop of cigarettes and an important economic crop in China, and the variety is the basis of flue-cured tobacco production. For a long time, the main varieties used in my country's flue-cured tobacco production have been a few varieties such as Yunyan 87, K326, China Tobacco 100, and China Tobacco 103, resulting in a single planting structure and degradation of comprehensive disease resistance and stress resistance. In recent years, tobacco common mosaic disease (TMV), climatic spot disease, red spot disease, black shank disease and bacterial wilt have become more and more serious, seriously reducing the yield and quality of tobacco, and causing heavy economic losses. It has become a high-quality flue-cured tobacco in som...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 曹景林程君奇吴成林李亚培
Owner HUBEI TOBACCO SCI RES INST
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