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Breeding method of multi-resistant flue-cured tobacco three-cross

A three-cross, resistance technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of poor quality and adaptability, can not simultaneously resist foliar diseases and rhizome diseases, etc. The effect of breeding efficiency, solving poor quality and wide adaptability

Inactive Publication Date: 2017-07-07
HUBEI TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, my country's tobacco breeding units have bred a number of flue-cured tobacco hybrids, such as Yunyan 201, Jiyan 9, Liaoyan 16, etc., but these hybrids are all single-cross, and they only have yield advantages, and cannot simultaneously resist leaf tobacco. Surface diseases and rhizome diseases, poor quality and adaptability

Method used

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  • Breeding method of multi-resistant flue-cured tobacco three-cross
  • Breeding method of multi-resistant flue-cured tobacco three-cross
  • Breeding method of multi-resistant flue-cured tobacco three-cross

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Obtainment of inbred pure lines of flue-cured tobacco parent materials K730, RG11, G80, and Coker176

[0024] The Hubei Academy of Tobacco Science utilizes southern propagation and local breeding bases, and uses the flue-cured tobacco varieties K730, RG11 and G80 introduced from the United States as original resources. "Bacteria wilt" is the breeding goal, and the self-purification of selected strains is carried out alternately in the southern and northern environments with large climate differences. After 7 generations of self-purification, genetically stable inbred lines of K730, RG11 and G80 are obtained. ;Using the flue-cured tobacco variety Coker176 imported from the United States as the original resource, according to the breeding goal of "good quality and comprehensive economic traits, and resistance to TMV, climate spot, black shank and bacterial wilt" as the breeding goal, it has been continuously cultivated in southern China with large climate differ...

Embodiment 2

[0025] Example 2: Acquisition of flue-cured tobacco parent materials MS K730, MS RG11, MS G80, and MS K326

[0026]Hubei Institute of Tobacco Science used the flue-cured tobacco male sterile line MS G28 as the female parent, and used the K730 inbred line, RG11 inbred line, G80 inbred line obtained in Example 1 and conventional varieties used in production K326 was used as the male parent, and the male sterile F1 generation MS G28 / K730 self-bred line, MS G28 / RG11 self-bred line, MS G28 / G80 self-bred line and MS G28 / K326 were obtained by crossing. In the local breeding base, the male sterile F1 generation is used as the recurrent parent, and the corresponding male sterile F1 generation is continuously selected and backcrossed alternately in the southern and northern environments with large climate differences. After 8 generations of backcrossing The flue-cured tobacco male sterile lines MS K730, MS RG11, MS G80 and MS K326 were obtained. The quality, comprehensive economic trai...

Embodiment 3

[0027] Embodiment three: the preparation of three crosses

[0028] (1) Preparation of three-cross hybrid HB129

[0029] In the normal tobacco growing season of the breeding base of Hubei Tobacco Research Institute, the K730 inbred pure line and the Coker176 inbred pure line obtained in Example 1 were used as the female parent and the male parent respectively to prepare a fertile single-cross combination K730 inbred pure line / Coker176 inbred pure line. In the same year, in the Southern Breeding Base, the male sterile line MS K326 obtained in Example 2 was used as the female parent, and the above-mentioned fertile single-cross combination K730 self-bred pure line / Coker176 self-bred pure line was used as the male parent, and the three-cross The species MS K326 / / K730 inbred line / Coker176 inbred line, numbered HB129.

[0030] (2) Preparation of three-cross hybrid HB165

[0031] In the normal tobacco growing season of the breeding base of Hubei Provincial Tobacco Research Instit...

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Abstract

The invention provides a multi-resistance flue-cured tobacco three-cross breeding method. First, the conventional germplasm material A or its male sterile line B with good quality and comprehensive economic traits and resistance to red spot, black shank and bacterial wilt was used as the female parent. The conventional germplasm material C of climate spot, black shank and bacterial wilt is the male parent, and the single-cross combination A / C or B / C is used; The conventional variety D for black shank and bacterial wilt is the male parent, the single-cross combination B / C is the female parent, and the three-cross hybrid B / C / / D is prepared, or the male sterile line E of the conventional variety D is the female parent In this paper, the single-cross combination A / C was used as the male parent to prepare a three-cross variety E / / A / C. The method can effectively integrate the excellent genes of different parental materials, and the bred three-cross variety has the characteristics of good quality, wide adaptability, good comprehensive economic properties, and resistance to various root and stem diseases and foliar diseases.

Description

technical field [0001] The invention belongs to the technical field of tobacco breeding, and in particular relates to a multi-resistance flue-cured tobacco three-cross breeding method. Background technique [0002] Flue-cured tobacco is the main raw material crop of cigarettes and an important economic crop in China, and the variety is the basis of flue-cured tobacco production. For a long time, the main varieties used in my country's flue-cured tobacco production have been a few varieties such as Yunyan 87, K326, China Tobacco 100, and China Tobacco 103, resulting in a single planting structure and degradation of comprehensive disease resistance and stress resistance. In recent years, tobacco common mosaic disease (TMV), climatic spot disease, red spot disease, black shank disease and bacterial wilt have become more and more serious, seriously reducing the yield and quality of tobacco, and causing heavy economic losses. It has become a high-quality flue-cured tobacco in som...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 曹景林程君奇吴成林李亚培
Owner HUBEI TOBACCO SCI RES INST
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