Rapid identification of soybean anti-powdery mildew gene by using candidate gene strategy

A powdery mildew-resistant gene and powdery mildew-resistant technology can be applied in application, genetic engineering, plant genetic improvement and other directions, which can solve the problems of long time and difficulty in cloning, and achieve the effects of shortening time, shortening excavation cycle, and improving identification efficiency.

Inactive Publication Date: 2015-05-06
JIANGSU CHANGSHU MODERN AGRI IND PARK DEV
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Problems solved by technology

However, because the basic research on soybeans is not deep enough, it is not only time-consuming to use these methods but also difficult to accurately clone these genes

Method used

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  • Rapid identification of soybean anti-powdery mildew gene by using candidate gene strategy
  • Rapid identification of soybean anti-powdery mildew gene by using candidate gene strategy
  • Rapid identification of soybean anti-powdery mildew gene by using candidate gene strategy

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Embodiment Construction

[0030] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. The method can quickly identify soybean powdery mildew gene. The specific implementation process is as follows:

[0031] 1) Collection of soybean MLO-type genes

[0032] In order to obtain all the members of the soybean MLO gene family, we first constructed an HMM model using the MLO gene sequences of Arabidopsis thaliana, and the powdery mildew resistance MLO gene sequences of tomato, pea, rose, pepper, and lotus root, and collected them from the soybean genome sequence. Search for MLO-type genes; secondly, use the published MLO gene sequences in different crops as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC315947, TC325903, TC31594 TC315912,TC322759,TC322059,TC330654,TC282713,TC293173,TC281861,T...

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Abstract

The invention provides rapid identification of soybean anti-powdery mildew gene, relates to knowledge in the disciplines of plant comparative genomics, genetics and bioinformatics, and belongs to the scientific field of plant biotechnology. The invention includes the steps of: 1) downloading the soybean whole genome sequence and acquiring MLO type gene; 2) identifying the MLO genotype; 3) identifying MLO gene phylogenetic relationship; and 4) comparing MLO-type powdery mildew gene. The invention effectively shortens the gene mining cycle for soybean powdery mildew, and is conducive to the rapid identification of anti-powdery mildew gene; corresponding coseparation functional markers (SNP, scar, etc.) are developed through the identified candidate powdery mildew genes, and the identified candidate powdery mildew genes can also be used for fast molecular marker assisted selection of anti-powdery mildew genes and have high accuracy; and combined with other resistant gene molecular markers, multi-resistance breeding material can be created, so as to shorten the breeding period and improve breeding efficiency. Therefore, the invention lays foundation for the explanation of powdery mildew resistant molecular mechanism of soybean.

Description

technical field [0001] The present invention is to quickly identify the soybean powdery mildew gene by using methods such as plant comparative genomics, genetics, bioinformatics and candidate gene strategies by means of sequencing the whole genome sequence of soybean, and mainly involves the download of the whole genome sequence of soybean and the identification of candidate genes , gene comparison, clustering and other means, and then identify the powdery mildew gene, which belongs to the field of plant biotechnology science. Background technique [0002] Soybean powdery mildew is a disease caused by fungi. It is one of the main diseases affecting soybean production in the world, and it is also a common disease that threatens soybean production in my country. Powdery mildew mainly harms soybean leaves, not pods, and petioles and stems are rarely affected. In the early stage of infection, round spots appear on the leaves with dark green halos. Afterwards, the white powder ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G06F19/24C12N15/29C07K14/415
CPCC07K14/415C12Q1/6895C12Q2600/13G16B40/00
Inventor 钱春桃其他发明人请求不公开姓名
Owner JIANGSU CHANGSHU MODERN AGRI IND PARK DEV
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