Rapid identification on MLO type anti-disease gene of rice

Abstract

The invention discloses a method for rapidly identifying an anti-powdery mildew gene of rice, relates to scientific knowledge of plant comparative genomics, genetics and bioinformatics, and belongs to the field of plant biological technological science. The method mainly comprises the following steps: 1) downloading of a whole genome sequence of rice and acquiring the MLO type gene; 2) identifying the MLO type gene; 3) confirming the phylogenetic relationship of the MLO type gene; 4) comparing the MLO type anti-powdery mildew gene. By adopting the method, the powdery mildew gene discovery period of rice is effectively shortened, rapid identification on the powdery mildew gene is facilitated, a corresponding coseparation functional mark (SNP, SCAR and the like) can be developed by identifying the powdery mildew gene, and rapid marker-assisted selection on the anti-powdery mildew gene can be also performed with high accuracy; in combination with other anti-disease gene molecular marks, innovation of multi-resistance breeding materials can be achieved, the breeding years are shortened, and the breeding efficiency is improved; a basis is made for expressing the anti-powdery mildew molecular mechanism.

Description

technical field [0001] The present invention is to quickly identify the rice powdery mildew gene by using methods such as plant comparative genomics, genetics, bioinformatics and candidate gene strategies by means of sequencing the whole genome sequence of rice, and mainly involves the download of the whole genome sequence of rice and the identification of candidate genes , gene comparison, clustering and other means, and then identify the powdery mildew gene, which belongs to the field of plant biotechnology science. Background technique [0002] Powdery mildew is a common fungal disease of gramineous crops. During the disease occurrence period, the surface of leaves, stems and ears will be covered with a layer of white powdery mold, so that the normal metabolism of plants is disordered, causing premature aging and loss of yield. The control methods for these diseases in production mainly include pulling out susceptible plants, improving management models and adopting chemi...

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Problems solved by technology

However, because the basic research on rice is not deep enough, it is not only time-consuming to use these methods but also difficult to accurately clone these genes

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