19 results about "Gene Discovery" patented technology

The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel elements that further improve expression. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.

The invention belongs to the technical field of cell engineering and genetic engineering, and particularly relates to application of an FBXO32 gene in regulation and control of skeletal muscle satellite cell proliferation and feed utilization rate. According to overexpression of the FBXO32 gene in skeletal muscle satellite cells, it is found that the mRNA expression quantity of skeletal muscle satellite cell proliferation marker genes KI67 and CCND1 is remarkably increased, and the protein expression level is also remarkably improved. CCK-8 and EdU detection results also show that the FBXO32 gene or the encoded protein thereof also has a promotion effect on skeletal muscle satellite cell proliferation. The overexpressed FBXO32 gene or the encoded protein thereof can promote the proliferation of skeletal muscle satellite cells, and lays a foundation for improving meat traits of livestock and poultry, improving the feed utilization rate of the livestock and poultry and improving the meat traits of the livestock and poultry.

The present invention refers to hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB). The invention further refers to corresponding nucleic acids producing these variants, to a gene transfer system for stably introducing nucleic acid(s) into the DNA of a cell by using these hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB) and to transposons used in the inventive gene transfer system, comprising a nucleic acid sequence with flanking repeats (IRs and / or RSDs). Furthermore, applications of these transposase variants, the transpsoson, or the gene transfer system are also disclosed such as gene therapy, insertional mutagenesis, gene discovery (including genome mapping), mobilization of genes, library screening, or functional analysis of genomes in vivo and in vitro. Finally, pharmaceutical compositions and kits are also encompassed.

The invention discloses a rapid and efficient pear and apple canker resistance evaluation method, and relates to the technical field of horticultural plant disease resistance evaluation.The method comprises the following steps of S1, test branch preparation, S2, pathogenic bacterium culture, S3, contact method inoculation and S4, resistance evaluation.The method can accurately evaluate the canker resistance of pear and apple resources, and the method has the advantages of being high in practicability and high in practicability. The method has the advantages of simple operation, no need of punching or preparation of fungus cakes, simplified operation steps, completion of inoculation of 100-120 parts of materials within 2 hours, efficiency more than 10 times of that of a traditional punching method, suitableness for rapid screening of large-scale germplasm resources, suitableness for canker resistance evaluation of different varieties of pears and apples, germplasm resources and filial generations, and wide application prospect. And a technical support is provided for disease-resistant variety breeding, resistance gene mining and green prevention and control.

The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.

The invention discloses a pear PbrGA2ox1-1 gene and an application of the pear PbrGA2ox1-1 gene. The invention relates to a PbrGA2ox1-1 gene which is separated from Dangshan pear and is capable of regulating formation of lignin in stone cells of pear fruits. The nucleotide sequence of the PbrGA2ox1-1 gene is shown as SEQ ID No.1. According to the present invention, the PbrGA2ox1-1 gene is subjected to instantaneous overexpression in the pear fruit by using the agrobacterium instantaneous transformation method, the lignin content in the pulp is significantly reduced, and compared with the wild type pear pulp callus, the lignin content in the transgenic pear callus subjected to the overexpression of PbrGA2ox1-1 is significantly reduced, and the expression of a series of key genes in a lignin biosynthesis pathway is obviously inhibited. Therefore, the PbrGA2ox1-1 participates in regulating and controlling the formation of the lignin in the stone cells of the pear fruits. The discovery of the gene provides a new gene resource for fruit quality breeding, and the gene is an important candidate gene for improving fruit quality breeding in future genetic engineering.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of PP2C family genes in improving resistance of tomatoes to phytophthora infestans. Two genes related to phytophthora infestans infection are found and verified in tomatoes, the genome nucleotide sequences of the two genes are shown as SEQ ID NO: 1 and SEQ ID NO: 2 respectively, and the two genes belong to PP2C gene family members. The gene is knocked out or silenced through a gene editing technology, and it is found that the tomato strain with the gene knocked out or silenced has higher phytophthora infestans resistance compared with a wild type. Meanwhile, tomato strains edited by the genes are not obviously influenced in the aspects of growth and development. Therefore, the invention not only enriches the research of phytophthora infestans susceptibility related genes in tomatoes, but also develops a tomato strain with enhanced phytophthora infestans resistance by utilizing the characteristics of the genes, and provides a new technical strategy and gene resources for tomato breeding.

The application belongs to the technical field of molecular biology breeding, and particularly relates to application of EF Hand family genes in improving resistance of tomatoes to pathogenic Phytophthora. Two pathogenic Phytophthora disease-related genes are found and verified in tomatoes, the genomic nucleotide sequences of which are shown as SEQ ID NO: 1 and SEQ ID NO: 2 respectively, and the two genes both belong to members of the EF Hand gene family. By knocking out or silencing the above genes through gene editing technology, it is found that the tomato lines with the above genes knocked out or silenced have higher resistance to pathogenic Phytophthora compared with wild types. Meanwhile, the gene-edited tomato lines are not obviously affected in growth and development. Therefore, the application not only enriches the research on pathogenic Phytophthora disease-related genes in tomatoes, but also develops tomato lines with enhanced resistance to pathogenic Phytophthora by using the characteristics of the above genes, thereby providing new technical strategies and gene resources for tomato breeding.

The application discloses a multi-gene discovery network construction method and device, equipment and a storage medium. The method comprises the following steps: acquiring genome information input by a user, constructing a discovery network among all to-be-encoded genes according to the genome information, and determining storage information of each to-be-encoded gene; generating a DNA sequence according to the discovery network and the storage information, sequencing the DNA sequence, and obtaining a sequencing result; decoding the sequencing result, obtaining gene position information, judging whether there is an encoded gene according to the gene position information, quickly and efficiently finding the corresponding gene during decoding, saving the gene searching time, quickly detecting the gene report error, avoiding the sequencing result error caused by multiple variations and the sequencing itself, and improving the speed and efficiency of the multi-gene discovery network construction.

Embodiments of the present application provide a biomedical candidate gene discovery method based on text-graph fusion and related equipment. The method comprises: constructing a literature-derived semantic predicate graph (LDSPG) centered on a target disease; based on search enhancement generation, using a large language model to perform chain thinking reasoning on the evidence retrieved from the LDSPG, and curating high-quality training sample pairs; constructing a double-encoder model comprising a text encoder and a graph encoder, and aligning the text semantic space and the graph topological space to a unified embedding space through a hybrid contrastive loss function; merging the graph diffusion ranking and the double-encoder ranking through a reciprocal ranking fusion algorithm to generate a fusion ranking result; and based on an external biomedical database, performing deterministic evidence grading on the candidate genes and outputting an auditable candidate gene list. Through high-quality data curation, cross-modal information fusion, complementary ranking fusion and traceable evidence grading, the accuracy and explainability of candidate gene discovery are effectively improved.

The application discloses a transcription factor CuLQW1 of Chimonocalamus jinfushanensis and an encoding gene and application thereof, belongs to the technical field of plant genetic engineering, and particularly relates to an encoding gene of the transcription factor CuLQW1 of Chimonocalamus jinfushanensis, wherein the nucleotide sequence of the encoding gene is shown in SEQ ID NO. 2. The application discloses an application of the encoding gene of the transcription factor CuLQW1 of Chimonocalamus jinfushanensis in regulating synthesis of lignin and cellulose of Gramineae plants. The application discloses an application of the encoding gene of the transcription factor CuLQW1 of Chimonocalamus jinfushanensis in regulating drought stress tolerance of rice. The application successfully separates and identifies the lignin and cellulose synthesis regulation transcription factor CuLQW1 and the encoding gene thereof from Chimonocalamus jinfushanensis, finds that the lignin and cellulose synthesis regulation transcription factor CuLQW1 is involved in regulation of synthesis of lignin and cellulose, regulates drought stress tolerance of rice, and provides a new gene resource for plant material improvement genetic engineering.

This invention discloses the application of MIF gene silencing in the preparation of drugs for treating premature rupture of membranes (PPROM), belonging to the fields of biomedicine and molecular biology. By collecting fetal membrane tissues from patients with PPROM and normal controls, this invention found that the expression level of MIF protein and the concentration of PGE2 were significantly increased in the PPROM group. Further investigation using siRNA technology to silence the MIF gene in human amniotic epithelial cells revealed that MIF silencing significantly reduced PGE2 levels. This invention is the first to demonstrate that MIF gene silencing can effectively treat PPROM by reducing PGE2 (inhibiting uterine contractions). This invention also provides a pharmaceutical composition containing MIF siRNA. This invention provides a novel molecular target and therapeutic strategy for the treatment of PPROM, with broad clinical application prospects.

The present invention refers to hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB). The invention further refers to corresponding nucleic acids producing these variants, to a gene transfer system for stably introducing nucleic acid(s) into the DNA of a cell by using these hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB) and to transposons used in the inventive gene transfer system, comprising a nucleic acid sequence with flanking repeats (IRs and / or RSDs). Furthermore, applications of these transposase variants, the transposon, or the gene transfer system are also disclosed such as gene therapy, insertional mutagenesis, gene discovery (including genome mapping), mobilization of genes, library screening, or functional analysis of genomes in vivo and in vitro. Finally, pharmaceutical compositions and kits are also encompassed.

The application discloses a kind of for detecting sheep milk production character FGF2 Gene SNPs marker and its application belong to the field of biotechnology.The present application takes east frier milk sheep as research object, and the nucleotide sequence variation site of gene is detected by applying PARMS, Sanger and other technologies FGF2 General linear mixed effect model is constructed, the correlation between sequence variation and sheep lactation character is researched, and molecular genetic marker for regulating sheep lactation character is excavated.The present application has achieved some positive effects, and indeed has great advantages compared with prior art;in FGF2 Gene discovers SNPs site c.178+11694 T / C;The milk production character of test sheep of SNP site 1 c.178+11694 T / C genotype CC or CT is better than or auxiliary better than the test sheep of SNP site 1: c.178+11694 T / C genotype TT.

The invention discloses a resistance gene discovery method based on a graph neural network, and relates to the technical field of bioinformatics and artificial intelligence crossing, and the method comprises the following steps: obtaining metagenome sequencing data, and loosely comparing the metagenome sequencing data with a resistance gene database to obtain candidate sequences; constructing an undirected graph; extracting an initial vector feature of the candidate sequence by using a DNA language model, and inputting the undirected graph and the initial vector feature into a graph neural network to obtain a context sensing feature vector of each node; identifying connected sub-graphs in the undirected graph; aggregating the context sensing feature vectors of the nodes in each connected sub-graph into global feature vectors; and inputting the global feature vector into a classifier, and determining a connected subgraph belonging to the drug-resistant gene. According to the novel method, limitation of genome assembly can be avoided, fragmented sequence information can be effectively integrated, and reliable discovery of drug-resistant genes, especially low-abundance and novel drug-resistant genes, is realized.

The invention provides a gene mutant capable of overcoming distant hybridization lethal and a detection primer thereof. According to the gene mutant capable of overcoming distant hybridization lethal, an NtHL1S gene is mutated, and bases at the 472nd site and the 473rd site are replaced with A; the gene mutant directly participates in regulation and control, and the distant hybridization lethal phenomenon, especially the hybridization lethal phenomenon between common tobacco and African tobacco, is overcome by inhibiting expression of the NtHL1S gene in the tobacco for the first time. According to the method disclosed by the invention, the tobacco gene NtHL1S is mutated by utilizing a CRISPR / Cas9 method, and the situation that the hybridization lethal phenomenon between common tobacco and African tobacco disappears due to the mutation of the NtHL1S gene is found. A primer combination is developed aiming at an NtHL1S gene mutation site, and is used for transformation of a target site for gene identification, and rapid screening of a target single plant in molecular marker-assisted selective breeding offspring materials.

This invention belongs to the fields of plant molecular biotechnology and genetic engineering, specifically relating to the application of an expression promoter of transcription factor DkAS1 in regulating the ripening and softening of persimmon fruit. The amino acid sequence of DkAS1 is shown in SEQ ID NO.1. This invention utilizes biotechnology to isolate the gene encoding the transcription factor DkAS1 in persimmon fruit and discovers... DkAS1 It is induced by gibberellin treatment and can inhibit cell wall degradation, ethylene and abscisic acid synthesis, and participate in the regulation of the postharvest softening process of persimmon fruit.

The invention relates to the technical field of plant genetic engineering and molecular breeding, and discloses a fructokinase MdFRK1 gene and application thereof in regulation and control of apple fructose accumulation. The apple fructokinase MdFRK1 gene is subjected to naming, structural domain analysis, promoter activity analysis, gene cloning and subcellular localization analysis, recombinant protein is obtained through induced expression of escherichia coli, enzyme activity is detected, fructose content change is analyzed after the MdFRK1 gene is silenced through a VIGS experiment, and results show that the MdFRK1 is mainly localized in cytoplasm, and the MdFRK1 gene can be used for detecting fructose content change. The fructokinase is low-affinity fructokinase, and analysis on activity of the MdFRK1 promoter induced by different sugar sources shows that the activity of the MdFRK1 promoter is the highest under fructose induction; the MdFRK1 gene is silenced through a VIGS experiment, it is found that the activity of fructokinase and the content of fructose are both reduced, and it is found that the activity of fructokinase and the content of fructose are both increased through overexpression of the MdFRK1 gene. According to the invention, the gene positively correlated with the fructose content of the apple is found for the first time, and a new method is provided for culturing the apple with high fructose content.