42 results about "Gene Discovery" patented technology

The invention relates to the technical field of biological breeding, in particular to application of an RBX1 gene in cow ovarian granular cells. According to the invention, the RBX1 gene is specifically regulated on the ovarian granulosa cells, and the RBX1 gene is found to have the effect of promoting the proliferation of the granulosa cells; and the gene is provided for the creation of a new high-fecundity breeding material for gene editing cattle in the later period.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of RIN4 family genes in improving resistance of tomatoes to phytophthora infestans. Two genes related to phytophthora infestans infection are found and verified in tomatoes, the genome nucleotide sequences of the two genes are shown as SEQ ID NO: 1 and SEQ ID NO: 2 respectively, and the two genes belong to RIN4 gene family members. The gene is knocked out or silenced through a gene editing technology, and it is found that the tomato strain with the gene knocked out or silenced has higher phytophthora infestans resistance compared with a wild type. Meanwhile, tomato strains edited by the genes are not obviously influenced in the aspects of growth and development. Therefore, the invention not only enriches the research of phytophthora infestans susceptibility related genes in tomatoes, but also develops a tomato strain with enhanced phytophthora infestans resistance by utilizing the characteristics of the genes, and provides a new technical strategy and gene resources for tomato breeding.

The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel elements that further improve expression. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.

The invention belongs to the technical field of cell engineering and genetic engineering, and particularly relates to application of an FBXO32 gene in regulation and control of skeletal muscle satellite cell proliferation and feed utilization rate. According to overexpression of the FBXO32 gene in skeletal muscle satellite cells, it is found that the mRNA expression quantity of skeletal muscle satellite cell proliferation marker genes KI67 and CCND1 is remarkably increased, and the protein expression level is also remarkably improved. CCK-8 and EdU detection results also show that the FBXO32 gene or the encoded protein thereof also has a promotion effect on skeletal muscle satellite cell proliferation. The overexpressed FBXO32 gene or the encoded protein thereof can promote the proliferation of skeletal muscle satellite cells, and lays a foundation for improving meat traits of livestock and poultry, improving the feed utilization rate of the livestock and poultry and improving the meat traits of the livestock and poultry.

The present invention refers to hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB). The invention further refers to corresponding nucleic acids producing these variants, to a gene transfer system for stably introducing nucleic acid(s) into the DNA of a cell by using these hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB) and to transposons used in the inventive gene transfer system, comprising a nucleic acid sequence with flanking repeats (IRs and / or RSDs). Furthermore, applications of these transposase variants, the transpsoson, or the gene transfer system are also disclosed such as gene therapy, insertional mutagenesis, gene discovery (including genome mapping), mobilization of genes, library screening, or functional analysis of genomes in vivo and in vitro. Finally, pharmaceutical compositions and kits are also encompassed.

This invention discloses a method for evaluating the salt tolerance of mulberry germplasm resources and screening salt-tolerant germplasm, belonging to the field of germplasm resource evaluation and screening technology. The method includes sampling and storage of experimental materials, explant preparation, disinfection, culture, salt tolerance identification, evaluation of salt tolerance using membership functions and cluster analysis, and comprehensive analysis of the salt tolerance of mulberry winter buds. This invention identifies the salt tolerance of mulberry planting resources collected from different regions, using the bud germination status index and the growth rate of relatively strong buds as evaluation indicators for screening a large number of mulberry materials for salt tolerance identification. Membership functions and cluster analysis are used for comprehensive evaluation of salt tolerance, screening out different salt-tolerant germplasm and providing excellent germplasm resources for subsequent salt tolerance gene discovery and salt-tolerant variety breeding.

This invention relates to the field of biological breeding technology, and particularly to... RBX1 Application of genes in bovine ovarian granulosa cells. This invention specifically regulates genes in ovarian granulosa cells. RBX1 Genes, Discovery RBX1 The gene has the function of promoting the proliferation of granulosa cells; it provides genes for the creation of new high-fertility breeding materials for gene-edited cattle in the later stage.

The present invention discloses an interfering RNA of TPPP3 and its application, belonging to the field of biomedicine preparation technology. The present invention discloses the application of a preparation for inhibiting TPPP3 in the preparation of a drug for treating type I hypersensitivity reaction, the preparation comprising an siRNA that interferes with the expression of TPPP3, and the nucleotide sequence of the siRNA is as shown in SEQ ID NO: 5-6. The present invention knocks down the TPPP3 gene by RNAi technology, and finds that downregulating the expression of the TPPP3 gene can significantly inhibit IgE-mediated mast cell degranulation, and reduces the activity of upstream signal molecules of the FcεRI signaling pathway to regulate type I hypersensitivity reaction, providing an experimental basis and scientific basis for revealing the pathogenesis of type I hypersensitivity reaction and in-depth research on the role of TPPP3 in type I hypersensitivity reaction.

The application belongs to the technical field of molecular biology breeding, and particularly relates to application of RIN4 family genes in improving resistance of tomatoes to Phytophthora infestans. Two Phytophthora infestans disease-related genes are found and verified in tomatoes, the genomic nucleotide sequences of which are shown as SEQ ID NO: 1 and SEQ ID NO: 2 respectively, and the two genes both belong to members of the RIN4 gene family. By knocking out or silencing the above genes through gene editing technology, it is found that the tomato lines with the above genes knocked out or silenced have higher resistance to Phytophthora infestans compared with wild types. Meanwhile, the gene-edited tomato lines are not obviously affected in growth and development. Therefore, the application not only enriches the research on Phytophthora infestans disease-related genes in tomatoes, but also develops tomato lines with enhanced resistance to Phytophthora infestans by using the characteristics of the above genes, thereby providing new technical strategies and gene resources for tomato breeding.

The invention discloses a rapid and efficient pear and apple canker resistance evaluation method, and relates to the technical field of horticultural plant disease resistance evaluation.The method comprises the following steps of S1, test branch preparation, S2, pathogenic bacterium culture, S3, contact method inoculation and S4, resistance evaluation.The method can accurately evaluate the canker resistance of pear and apple resources, and the method has the advantages of being high in practicability and high in practicability. The method has the advantages of simple operation, no need of punching or preparation of fungus cakes, simplified operation steps, completion of inoculation of 100-120 parts of materials within 2 hours, efficiency more than 10 times of that of a traditional punching method, suitableness for rapid screening of large-scale germplasm resources, suitableness for canker resistance evaluation of different varieties of pears and apples, germplasm resources and filial generations, and wide application prospect. And a technical support is provided for disease-resistant variety breeding, resistance gene mining and green prevention and control.

The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.

The invention discloses a BR response tea tree COR413 gene promoter, which can be used for efficiently promoting the expression of a COR413 gene. The nucleotide sequence of the promoter is shown in figure 2, and an upstream regulatory sequence of a tea tree COR413 gene is cloned to obtain a complete sequence of a basic element of the promoter. The promoter can regulate and control the expression of an arabidopsis thaliana GUS gene. The verification of the interaction of the BR transcription factors CsBZR2-1 and CsCOR413 is carried out by utilizing an EMSA (Empirical Mode Serum Albumin) experiment, and a regulation and control site is determined. Meanwhile, the CsCOR413 gene in tea tree leaves is silenced through a VIGS technology, and it is found that the CsCOR413 gene positively regulates and controls the cold resistance of tea trees. The gene has very important significance for fundamental research and production practice of tea germplasm innovation by using the gene in the future.

The invention relates to the technical field of biology, in particular to a colorectal cancer prognosis marker, a prognosis prediction model and a construction method of the colorectal cancer prognosis marker. Gene expression data of a colorectal cancer patient and single-cell RNA sequencing data of a tumor immune single-cell center are obtained from a cancer genome map and a gene expression comprehensive database, and 224 hypoxia and fructose metabolism related genes are selected; a hypoxia and fructose metabolism related gene scoring system is constructed through regression analysis and various machine learning algorithms, a risk model is constructed, eight genes significantly related to poor prognosis are preliminarily selected from the risk model, and further verification is performed in combination with TCGA transcriptome data and a protein expression profile of a human protein map database. Kaplan-Meier survival curve, multi-factor regression analysis, column diagram construction and ROC curve evaluation are carried out, key prognosis genes of P4HA1, ANKZF1 and STC2 are determined, it is found that sabutok can be combined with ANKZF1, STC2 and P4HA1 in a high-affinity mode, and sabutok is revealed to serve as a promising treatment choice for high-risk patients.

The invention discloses a pear PbrGA2ox1-1 gene and an application of the pear PbrGA2ox1-1 gene. The invention relates to a PbrGA2ox1-1 gene which is separated from Dangshan pear and is capable of regulating formation of lignin in stone cells of pear fruits. The nucleotide sequence of the PbrGA2ox1-1 gene is shown as SEQ ID No.1. According to the present invention, the PbrGA2ox1-1 gene is subjected to instantaneous overexpression in the pear fruit by using the agrobacterium instantaneous transformation method, the lignin content in the pulp is significantly reduced, and compared with the wild type pear pulp callus, the lignin content in the transgenic pear callus subjected to the overexpression of PbrGA2ox1-1 is significantly reduced, and the expression of a series of key genes in a lignin biosynthesis pathway is obviously inhibited. Therefore, the PbrGA2ox1-1 participates in regulating and controlling the formation of the lignin in the stone cells of the pear fruits. The discovery of the gene provides a new gene resource for fruit quality breeding, and the gene is an important candidate gene for improving fruit quality breeding in future genetic engineering.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of PP2C family genes in improving resistance of tomatoes to phytophthora infestans. Two genes related to phytophthora infestans infection are found and verified in tomatoes, the genome nucleotide sequences of the two genes are shown as SEQ ID NO: 1 and SEQ ID NO: 2 respectively, and the two genes belong to PP2C gene family members. The gene is knocked out or silenced through a gene editing technology, and it is found that the tomato strain with the gene knocked out or silenced has higher phytophthora infestans resistance compared with a wild type. Meanwhile, tomato strains edited by the genes are not obviously influenced in the aspects of growth and development. Therefore, the invention not only enriches the research of phytophthora infestans susceptibility related genes in tomatoes, but also develops a tomato strain with enhanced phytophthora infestans resistance by utilizing the characteristics of the genes, and provides a new technical strategy and gene resources for tomato breeding.

The invention discloses a rice grain type protein Os03g0122600 as well as a coding gene and application thereof. The rice grain type protein Os03g0122600 has an amino acid sequence as shown in SEQ ID NO.2, or is a functional equivalent variant formed by substitution, deletion or addition of one or more amino acid residues in the sequence as shown in SEQ ID NO.2. The invention also discloses a preparation method of the rice grain type protein Os03g0122600. The coding gene has a nucleotide sequence as shown in SEQ ID NO.1, or a nucleotide sequence which is hybridized with SEQ ID NO.1 and codes the same functional protein. The Os03g0122600 gene is knocked out through a CRISPR / Cas9 gene editing technology, it is found that compared with a wild type, the rice grain length is remarkably increased, the grain length-width ratio and the thousand grain weight are increased, and a new strategy is provided for molecular breeding of high-yield and high-quality rice varieties.

The machine learning system employs a causal discovery method to identify genes that cause colorectal cancer when affected by genome alteration. The co-expression pattern between their target differentially expressed genes (DEGs) is found to construct a set of "meta-genes" such that their expression values reflect the status of the cellular signaling system. The tumor is represented using meta-genes as features, and a classification model is trained to predict whether tumor cells of a patient are sensitive to chemotherapy and biological drugs.

The invention provides a gene mutant for overcoming tobacco distant hybridization lethal and a related method. The invention relates to a gene mutant capable of overcoming tobacco distant hybridization lethal, which is obtained by mutating an NtHL1 S gene and inserting two bases TT behind a 473rd base. The gene mutant can overcome death caused by far-source hybridization between common tobacco and African tobacco. According to the gene mutant, the problem of death caused by distant hybridization of tobacco is solved by inhibiting expression of the NtHL1 S gene in the tobacco for the first time. According to the method disclosed by the invention, the tobacco gene NtHL1 S is mutated by utilizing a CRISPR / Cas9 method, and the situation that the hybridization lethal phenomenon between common tobacco and African tobacco disappears due to the mutation of the NtHL1 S gene is found. A primer combination is developed aiming at NtHL1 S gene mutation sites and is used for genotype identification of transformation target sites and rapid screening of target single plants in molecular marker-assisted selective breeding offspring materials.

The application belongs to the technical field of molecular biology breeding, and particularly relates to application of EF Hand family genes in improving resistance of tomatoes to pathogenic Phytophthora. Two pathogenic Phytophthora disease-related genes are found and verified in tomatoes, the genomic nucleotide sequences of which are shown as SEQ ID NO: 1 and SEQ ID NO: 2 respectively, and the two genes both belong to members of the EF Hand gene family. By knocking out or silencing the above genes through gene editing technology, it is found that the tomato lines with the above genes knocked out or silenced have higher resistance to pathogenic Phytophthora compared with wild types. Meanwhile, the gene-edited tomato lines are not obviously affected in growth and development. Therefore, the application not only enriches the research on pathogenic Phytophthora disease-related genes in tomatoes, but also develops tomato lines with enhanced resistance to pathogenic Phytophthora by using the characteristics of the above genes, thereby providing new technical strategies and gene resources for tomato breeding.

The application discloses a multi-gene discovery network construction method and device, equipment and a storage medium. The method comprises the following steps: acquiring genome information input by a user, constructing a discovery network among all to-be-encoded genes according to the genome information, and determining storage information of each to-be-encoded gene; generating a DNA sequence according to the discovery network and the storage information, sequencing the DNA sequence, and obtaining a sequencing result; decoding the sequencing result, obtaining gene position information, judging whether there is an encoded gene according to the gene position information, quickly and efficiently finding the corresponding gene during decoding, saving the gene searching time, quickly detecting the gene report error, avoiding the sequencing result error caused by multiple variations and the sequencing itself, and improving the speed and efficiency of the multi-gene discovery network construction.

The invention belongs to the technical field of biotechnology and livestock breeding, and relates to an SNP molecular marker influencing the breeding traits of goats and application, the nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.1, the 102bp site in the SEQ ID NO.1 is G or C, and the breeding traits refer to the multiparous lambing number. According to the invention, the effective SNP molecular marker influencing the lambing number of the goats is found on the LGR4 gene for the first time and is used for identifying the multiparous lambing number of the goats. The goats are screened through the molecular marker, GG homozygous genotype individuals are reserved, CC homozygous genotype individuals and CG heterozygous genotype individuals are eliminated, and the reproductive performance of the goats can be improved.

The application belongs to the field of biotechnology and livestock breeding technology, and relates to a SNP molecular marker affecting goat reproductive traits and application, wherein the nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO. 1, the 102th site in the sequence of SEQ ID NO. 1 is G or C, and the reproductive trait refers to the number of offspring of a goat. LGR4 The application finds an effective SNP molecular marker affecting the number of offspring of a goat, and is used for identifying the number of offspring of a goat. By screening goats through the molecular marker, GG homozygous genotype individuals are retained, and CC homozygous genotype individuals and CG heterozygous genotype individuals are eliminated, so that the reproductive performance of the goat can be improved.

The present invention belongs to the field of medicine, and specifically relates to a lncPostn as a biomarker for predicting cardiac fibrosis and its application. The nucleotide sequence of the lncPostn gene is shown in SEQ ID NO: 1. The present invention first discovered the lncPostn gene, found that it is homologous to humans and mice, and is expected to become a new target for the treatment of cardiac fibrosis after myocardial infarction.

The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.

The present invention provides the use of the FKBP5 gene as a molecular marker for photon radiation resistance in nasopharyngeal carcinoma, belonging to the field of biomedicine. Through protein profiling, gene sequencing, and clinical sample gene chip testing, the present invention analyzes and screens genes differentially expressed in nasopharyngeal carcinoma radiation-resistant and radiation-sensitive cell lines. The findings indicate that FKBP5 expression is significantly higher in radiation-resistant nasopharyngeal carcinoma cells than in radiation-sensitive nasopharyngeal carcinoma cells. Inhibiting FKBP5 expression significantly enhances nasopharyngeal carcinoma radiosensitivity and cell lethality.

Embodiments of the present application provide a biomedical candidate gene discovery method based on text-graph fusion and related equipment. The method comprises: constructing a literature-derived semantic predicate graph (LDSPG) centered on a target disease; based on search enhancement generation, using a large language model to perform chain thinking reasoning on the evidence retrieved from the LDSPG, and curating high-quality training sample pairs; constructing a double-encoder model comprising a text encoder and a graph encoder, and aligning the text semantic space and the graph topological space to a unified embedding space through a hybrid contrastive loss function; merging the graph diffusion ranking and the double-encoder ranking through a reciprocal ranking fusion algorithm to generate a fusion ranking result; and based on an external biomedical database, performing deterministic evidence grading on the candidate genes and outputting an auditable candidate gene list. Through high-quality data curation, cross-modal information fusion, complementary ranking fusion and traceable evidence grading, the accuracy and explainability of candidate gene discovery are effectively improved.

The application discloses a transcription factor CuLQW1 of Chimonocalamus jinfushanensis and an encoding gene and application thereof, belongs to the technical field of plant genetic engineering, and particularly relates to an encoding gene of the transcription factor CuLQW1 of Chimonocalamus jinfushanensis, wherein the nucleotide sequence of the encoding gene is shown in SEQ ID NO. 2. The application discloses an application of the encoding gene of the transcription factor CuLQW1 of Chimonocalamus jinfushanensis in regulating synthesis of lignin and cellulose of Gramineae plants. The application discloses an application of the encoding gene of the transcription factor CuLQW1 of Chimonocalamus jinfushanensis in regulating drought stress tolerance of rice. The application successfully separates and identifies the lignin and cellulose synthesis regulation transcription factor CuLQW1 and the encoding gene thereof from Chimonocalamus jinfushanensis, finds that the lignin and cellulose synthesis regulation transcription factor CuLQW1 is involved in regulation of synthesis of lignin and cellulose, regulates drought stress tolerance of rice, and provides a new gene resource for plant material improvement genetic engineering.

This invention discloses the application of MIF gene silencing in the preparation of drugs for treating premature rupture of membranes (PPROM), belonging to the fields of biomedicine and molecular biology. By collecting fetal membrane tissues from patients with PPROM and normal controls, this invention found that the expression level of MIF protein and the concentration of PGE2 were significantly increased in the PPROM group. Further investigation using siRNA technology to silence the MIF gene in human amniotic epithelial cells revealed that MIF silencing significantly reduced PGE2 levels. This invention is the first to demonstrate that MIF gene silencing can effectively treat PPROM by reducing PGE2 (inhibiting uterine contractions). This invention also provides a pharmaceutical composition containing MIF siRNA. This invention provides a novel molecular target and therapeutic strategy for the treatment of PPROM, with broad clinical application prospects.

The present invention refers to hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB). The invention further refers to corresponding nucleic acids producing these variants, to a gene transfer system for stably introducing nucleic acid(s) into the DNA of a cell by using these hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB) and to transposons used in the inventive gene transfer system, comprising a nucleic acid sequence with flanking repeats (IRs and / or RSDs). Furthermore, applications of these transposase variants, the transposon, or the gene transfer system are also disclosed such as gene therapy, insertional mutagenesis, gene discovery (including genome mapping), mobilization of genes, library screening, or functional analysis of genomes in vivo and in vitro. Finally, pharmaceutical compositions and kits are also encompassed.