21 results about "Toxicology studies" patented technology

The invention provides a micro-fluidic chip system for fixing juvenile zebrafish with a matched injection pump facing upwards on the side / back surface as well as a preparation method and application of the micro-fluidic chip system. The micro-fluidic chip system for fixing the juvenile zebra fish with the matched injection pump facing upwards on the side / back surface comprises a conveying device, the juvenile zebra fish micro-fluidic chip and an analysis device, the conveying device comprises an injection pump and a conveying catheter. According to the side / back upward type micro-fluidic chip system for fixing the juvenile zebrafish by matching with the injection pump, and the preparation method and the application of the micro-fluidic chip system, provided by the invention, the juvenile zebrafish can be automatically fixed through automatic water flow injection under the condition of no anesthetic treatment by combining a fluid dynamic method with the shape of the juvenile zebrafish; meanwhile, the system has the advantages that high throughput can be realized, and potential technical support can be provided for further realizing drug screening, poison screening and toxicological research and related research of zebra fish.

The present disclosure relates to methods and compositions for generating topographically organized tissues in vitro, for the resulting cultured tissue and components thereof, and for uses of such cultured tissue and its components in drug discovery, toxicology studies, and therapy.

The invention belongs to the technical field of medicines, and discloses application of asarinol and derivatives thereof in treatment of the Dravet syndrome. The structural general formula of asarinol and the derivatives thereof is shown in (I). Researches find that in an scn1lab / zebra fish model, alpha-asarone, beta-asarone and derivatives thereof can reduce epilepsy-like discharge by 23%-93%, and the effect is obviously superior to that of stiripentanol and cannabidiol. Related pharmaceutical preparations can be prepared into capsules, dry suspensions or gels and the like, the dry suspensions are more suitable for children to take, and the capsules and the gels are suitable for adults to take. In addition, toxicological studies show that the compound is high in safety and free of remarkable developmental toxicity. The invention provides a safe and efficient novel treatment scheme for the Dravot syndrome and intractable epilepsy, and has important clinical transformation value. (I)

The invention discloses a diagnostic model construction method based on a novel network toxicology research normal form, and belongs to the field of health risk assess.The diagnostic model construction method specifically comprises the following steps that firstly, a potential poison and disease target library is constructed, and common potential targets between ATBC and fracture healing damage are screened and recognized; iI, constructing a PPI network to identify a core target, and analyzing functions and pathway enrichment of the core target; iII, identifying and screening the biomarkers, and constructing a diagnostic model to evaluate the diagnostic value of the biomarkers; according to the method, the evaluation precision of ATBC on the potential risk of fracture healing is remarkably improved, the conformation limitation of traditional static docking is broken through, stability evaluation in the time dimension is provided, the limitation of a rigid docking method is effectively overcome, meanwhile, visual representation of the dynamic behavior of a compound is provided, and the accuracy of the dynamic behavior of the compound is improved. The method provides a quantitative basis for mechanism analysis of molecular interaction, provides an effective technical means for subsequent high-risk substance screening, and is simple and convenient to operate and wide in application range.

The invention discloses a tobacco product lung injury evaluation method based on murine lung organs, which comprises the following steps: step A, preparing a trapping substance by using a tobacco product to obtain the tobacco product trapping substance; step B, performing induction culture on the mouse lung tissue by using an MAOs culture medium to obtain mouse lung organs; step C, pre-poisoning the lung organs of the mouse by using the tobacco product traps to determine IC50 and poisoning time of the tobacco product traps; and D, setting a concentration gradient according to the IC50 of the tobacco product traps, contaminating the lung organs of the mouse, and evaluating the lung injury caused by the tobacco product traps according to the physiological index change of the lung organs of the mouse. According to the method for evaluating the lung injury of the tobacco product by using the mouse lung organ, the influence of exposure of the tobacco product on the lung injury can be simulated, the effect difference between different tobacco products is reflected, and a more accurate evaluation method is provided for toxicological research of the tobacco product.

The invention discloses a method for quantitatively determining the internal exposure dose of carbonaceous particles in a living body based on an SDS-PAGE gray scale standard curve and application. Carrying out azeotropy on the carbonaceous particulate matter turbid liquid and 2 * Loading Buffer, and then carrying out electrophoresis, wherein the particles form a black strip on a 4% concentrated gel interface; establishing a gray scale-quality standard curve by using a series of quality gradients, namely converting the gray value of an unknown sample into absolute mass, and reporting by using mu g / mg prot as a unit; and calculating the mass concentration of the particulate matters in the lung tissue according to the actually measured concentration of the particulate matters in the animal alveolar lavage fluid cell sample or the human sputum cell sample by combining a pulmonary alveolar-lung tissue distribution coefficient K. The method is suitable for large-scale population exposure evaluation and animal toxicology research.

This invention relates to a medaka hatching enzyme, its preparation method, and its application. The preparation method of the medaka hatching enzyme includes the following steps: 1) collecting fertilized medaka eggs; 2) incubating the fertilized medaka eggs with embryo culture medium in a 26℃ incubator for 6 days, and collecting mature embryos; 3) adding NaCl solution and Tris-HCl buffer (pH=8.0) to the mature embryos, grinding them, collecting the filtrate, and allowing it to stand overnight at 4℃; 4) centrifuging the filtrate at 15000 rpm for 15 minutes, and extracting the supernatant to obtain the medaka hatching enzyme solution. The preparation method is simple to operate, and the obtained medaka hatching enzyme has high activity, which can help to quickly remove the medaka eggshell artificially. The naked medaka embryos obtained by this method can be directly used for in vivo imaging and in-situ quantitative analysis, and embryo model construction, providing support for subsequent genetic, developmental biological, and toxicological studies of medaka embryos.

The present disclosure relates to the development and application of a novel tumor adapter therapeutic drug. The therapeutic drug comprises a bispecific antibody binding to GPC3 and CD3, which comprises a first binding domain binding to GPC3 on the surface of target cells and a second binding domain binding to CD3 on the surface of T cells. The bispecific antibody can effectively inhibit the growth of transplanted tumors in immune-reconstituted mice at an extremely low dose, i.e. can inhibit tumor growth; the toxicology study in cynomolgus monkeys also shows that the animals are well tolerated to the bispecific antibody, and the bispecific antibody is superior to the same antibody in terms of efficacy and safety.

The utility model discloses a novel culture mould for 3d cell culture capable of being used for toxicological research, which belongs to the technical field of cell culture moulds and comprises a micropore mould, a partition mould is arranged on the micropore mould, a plurality of culture chambers are arranged inside the partition mould, a plurality of micro holes are arranged on the culture chambers, and the micro holes are communicated with the micropore mould. According to the three-dimensional cell culture mould, a plurality of cells with different drug concentrations can be cultured in the same mould at the same time, so that the operation amount and complexity of an experiment are greatly reduced, the preparation operation process of a three-dimensional cell structure is simplified, and the production efficiency is improved. According to the invention, the experimental error is reduced, the drug concentration can be ensured not to interfere with each other between partitions, the experimental efficiency is improved, a plurality of samples can be treated in the same mold at the same time, the experimental cost is reduced, and the consumption of reagents and materials is reduced.

The invention discloses fluorescent micro-nano plastic microspheres as well as a preparation method and application thereof, and belongs to the technical field of micro-nano plastic marking. The preparation method of the fluorescent micro-nano plastic microspheres comprises the following steps: dispersing polymer microspheres in a sodium dodecyl sulfate aqueous solution, and ultrasonically and uniformly mixing to obtain a polymer microsphere dispersion liquid; adding tetrahydrofuran, performing ultrasonic mixing, adding absolute methanol and fluorescent dye, performing ultrasonic mixing and stirring reaction, and performing reduced pressure distillation and washing after the reaction to obtain the fluorescent dye. The fluorescent micro-nano plastic microsphere is stable in fluorescence signal, high in fluorescence intensity and good in stability in an aqueous solution, the fluorescence intensity is not changed within 40 days, and the fluorescent micro-nano plastic microsphere can be applied to the fields of imaging tracing, toxicological research and the like and is wide in application prospect.

The invention discloses a micro-nano plastic analysis method based on nano hyperspectral fingerprint characteristics, and belongs to the technical field of environmental pollutant detection. The method comprises the following steps: establishing an initial spectrum library [SL1.0] containing a plurality of plastic polymers; optimizing the spectrum library through principal component analysis, extracting characteristic peaks, determining 600-840nm as a plastic fingerprint region, and constructing an optimized spectrum library [SL2.0]; preparing an atmospheric sedimentation sample or a biological tissue paraffin section; and collecting a hyperspectral image of the sample, and matching the hyperspectral image with the optimized spectrum library in the fingerprint region by using a spectrum angle matching algorithm to realize component identification and spatial positioning of the micro-nano plastic. According to the method, spectral redundancy and noise interference are effectively eliminated through PCA optimization, the multi-component identification accuracy is remarkably improved by focusing the fingerprint area, the form, component and distribution information of the micro-nano plastic can be obtained at the same time, and a reliable technical means is provided for environmental monitoring and toxicological research.

The application discloses a kind of experimental devices of toxicology research, it is related to the technical field of toxicology research, including first experimental cabinet and second experimental cabinet, the front end of the first experimental cabinet is provided with auxiliary frame, and the inside of auxiliary frame is installed with glove, the left and right sides of auxiliary frame are provided with auxiliary assembly for sealing effect detection of glove insertion.The application can utilize the design of air pump and connecting pipe in auxiliary assembly to inflate glove insertion before or after using glove insertion, and glove insertion is set as double layer, by inflating gas into glove insertion, the damage of glove insertion can be detected, to avoid glove insertion damage and use, and the glove insertion with damage can also be clamped and limited by auxiliary assembly, for emergency sealing treatment, to avoid the first experimental cabinet being connected with external environment through glove insertion, and reduce safety hazards.

The application discloses a method for preparing fine fusaric acid, which comprises the following steps: toxin production culture, sample extraction, preliminary purification, reverse-phase preparation, gel chromatography separation and normal-phase preparation. The method for preparing fine fusaric acid provided by the application improves material steady state and preparation efficiency, greatly reduces production cost, and has a purity of more than 99%, and can be used as a standard product for fine fusaric acid detection, prevention and control and toxicology research in food and feed.

The invention particularly relates to a multi-receptor and multi-level chlorinated paraffin toxicity effect database and visual expression system. The multi-receptor and multi-level chlorinated paraffin toxicity effect database comprises a data storage module used for storing sorted chlorinated paraffin query data; the data preprocessing module is used for converting and filtering the data; the index creating module is used for carrying out preprocessing and word segmentation on the text and constructing an inverted index; the data retrieval module is used for quickly retrieving data meeting conditions from the index; the chlorinated paraffin accumulation degree module is used for generating and displaying an accumulation degree point diagram and an accumulation degree thermodynamic diagram of chlorinated paraffin in an organism; the biological lesion map module is used for dynamically generating and displaying a lesion map in the living body contacted with the chlorinated paraffin according to an experimental result; and the video visualization module is used for generating and displaying a toxic action mechanism network of the chlorinated paraffin. According to the method, the chlorinated paraffin toxicity data retrieval efficiency is remarkably improved, the toxic action mechanism is visually displayed, and a precise visual analysis tool is provided for toxicological research.

The invention relates to the field of crystallization of organic compounds, and particularly discloses a crystal form 1 of deoxynivalenol (DON) and a preparation method of the crystal form 1. In an X-ray powder diffraction pattern, the crystal form shows characteristic diffraction peaks at positions of 2 theta angles of 8.80 degrees + / -0.2 degrees, 10.70 degrees + / -0.2 degrees, 11.33 degrees + / -0.2 degrees, 12.08 degrees + / -0.2 degrees, 15.54 degrees + / -0.2 degrees and the like. The crystal form 1 has the characteristics of high purity, good stability and the like, the preparation process is simple, the conditions are mild and controllable, the production cost is effectively reduced, and the practical application value is extremely high. According to the invention, the problems of unstable crystal form, low crystallization efficiency and the like of vomitoxin in the prior art are solved, and a high-purity standard product preparation scheme is provided for food safety detection, toxicological research and drug development.

The invention belongs to the technical field of new application of medicines, and particularly relates to application of itraconazole in preparation of a product for amplifying an induced T cell into a central memory T cell in vitro. It is found through experiments that itraconazole is additionally added into a CAR-T cell culture medium, CAR-T cells can be rapidly induced, differentiated and amplified into central memory CAR-T cells in vitro, and the proportion of the central memory CAR-T cells in the CAR-T cells is obviously increased. Itraconazole is a clinical medicine, pharmacokinetic and toxicological studies are clear, the safety is high, good technical support is provided for tumor cell treatment, and the itraconazole has important significance.

The invention discloses a culture system and a culture method for intestinal organs of euryhaline fish, and belongs to the technical field of bioengineering. The culture system is composed of a basic culture medium and a complete culture medium, wherein the basic culture medium is Advanced DMEM / F12, and is supplemented with a GlutaMax, Hepes and mycillin double-antibody solution; and on the basis of the complete culture medium, N-2, B-27, N-acetylcysteine, EGF (Epidermal Growth Factor), Noggin, R-Spondin 1 and other key cell factor combinations are further added. By optimizing the tissue pretreatment and digestion process, the efficient fish intestinal tract organoid culture and passage method is established, the organoid model with the crypt-villus-like structure, the cell heterogeneity and the intestinal tract barrier function is successfully constructed, and the model provides a highly bionic in-vitro research tool for fish physiology and toxicology research.

The application belongs to the technical field of new use of medicines, and particularly relates to application of itraconazole in preparation of a product for inducing T cells to be central memory T cells in vitro. It is found through experiments that, by additionally adding itraconazole in a CAR-T cell culture medium, CAR-T cells can be rapidly induced and differentiated to be central memory CAR-T cells in vitro, and the proportion of the central memory CAR-T cells in the CAR-T cells is obviously increased. Itraconazole is a clinical drug, and pharmacokinetics and toxicology research is clear, and the safety is high. The application provides good technical support for tumor cell treatment, and has important significance.

This invention relates to a method for screening key quality attributes of pharmaceuticals based on serious adverse reaction (SAR) data. It utilizes SAR data to establish appropriate indicators for classifying the risk levels of SARs among different products of the same drug variety. Furthermore, it establishes a comprehensive method for screening key quality attributes of pharmaceuticals based on SAR data to explore critical control points in the pharmaceutical manufacturing process. The developed method can indicate potential correlations between drug quality attributes and the probability of SAR occurrence, providing direction for further toxicological research and pharmaceutical quality standard research. The method is not limited by the range of quality attributes selected, seeking only intrinsic correlations within the existing scope, resulting in high reliability. By using correlation to replace causation, logistic regression reveals possible objective phenomena, providing directions for further toxicity evaluation and offering guidance and application value for pharmaceutical process improvement and standard enhancement.

The invention provides a ginkgo seed toxicity mechanism analysis method based on network toxicology, machine learning and experimental verification, and the ginkgo seed toxicity mechanism analysis method comprises screening and intersection analysis of ginkgo seed toxic component related targets and gastrointestinal tract toxicity related targets. Screening of gastrointestinal toxicity related differential expression genes (DEGs) and weighted gene co-expression network analysis (WGCNA); the method comprises the steps of key target spot selection, key target spot intersection analysis and function enrichment, key target spot screening and verification driven by machine learning, core gene function analysis, molecular docking and molecular dynamics simulation and experimental verification. The method breaks through the limitation that traditional toxicology research focuses on a single component or a target spot, constructs a component-target spot-disease network through a network toxicology method, captures complex interaction between toxic components and a biological system, and comprehensively reveals the multi-component and multi-target spot action characteristics of ginkgo seed toxicity.

The invention belongs to the technical field of medicines, and particularly relates to a preparation method of a tadalafil nitrosamine impurity. The invention discloses an efficient preparation method of two key nitrosamine impurity compounds of tadalafil. According to the method, nitrosation reaction is performed by adopting a specific reagent, directional synthesis of target impurities is realized under mild and controllable reaction conditions, and the method has the remarkable advantages of simplicity and convenience in operation, high product yield, high purity and the like. According to the method, the problem that related impurities are difficult to obtain is successfully solved, and a key material is provided for toxicological research or qualitative and quantitative research of the tadalafil nitrosamine impurities. The prepared impurity compound can be applied to nitrosamine genotoxic impurity detection and methodological development of tadalafil raw material medicines, preparations and intermediates thereof, and has important value for guaranteeing the safety and compliance of medicines.