86 results about "Toxicology studies" patented technology

This invention is linked to the chemical, pharmaceutical and cosmetic industries, where the bactericidal, virucidal, parasiticidal and fungicidal properties of the ozonized vegetable oils and fats are considered. The emulsified vegetable oil or fat is driven to react with ozone in a bubbling reactor, covered by a jacket that makes possible to control the reaction's temperature. Ozone reacts with the double bonds present in vegetable oil or fat, giving rise to the formation of different peroxidic species, responsible for the biological action of those products. The ozonized vegetable oils and fats in those conditions are applied with satisfactory and advanced results to the treatment of diseases in diverse medical specialties, such as ophthalmology, dentistry, dermatology, gastroenterology, gynecology, parasitology, and others. Likewise, the cosmetic application of the ozonized vegetable oil and fats is described, considering their oxygenating and revitalizing properties of the skin. The results of the toxicological studies previously performed show that the products obtained according to this process do not show any cross or adverse reaction.

A method of propagating mammalian endodermally derived progenitors such as hepatic progenitors, their progeny, or mixtures thereof is developed which includes culturing mammalian progenitors, their progeny, or mixtures thereof on a layer of embryonic mammalian feeder cells in a culture medium. The culture medium can be supplemented with one or more hormones and other growth agents. These hormones and other growth agents can include insulin, dexamethasone, transferrin, nicotinamide, serum albumin, beta-mercaptoethanol, free fatty acid, glutamine, CUSO4, and H2SeO3. The culture medium can also include antibiotics. Importantly, the culture medium does not include serum. The invention includes means of inducing the differentiation of the progenitors to their adult fates such as the differentiation of hepatic progenitor cells to hepatocytes or biliary cells by adding, or excluding epidermal growth factor, respectively. The method of producing mammalian progenitors is useful in that the progenitors can be used subsequently in one or more of the following processes: identification of growth and differentiation factors, toxicological studies, drug development, antimicrobial studies, or the preparation of an extracorporeal organ such as a bioartificial liver.

Biological samples undergo drastic changes during the execution of an experiment to aid in the determination of gene discovery, disease diagnosis, drug discovery, toxicological research, and so on. After a biological sample is processed, pieces of information about the state of a biological sample are generated. Pedigree information is also generated when a biological sample is sub-divided into multiple portions or when multiple biological samples are combined. These pieces of information illuminate the experiment process in a way that can help scientists to better understand the failure or the success of an experiment. Various pieces of information, such as the state and the pedigree of biological samples are tracked, traced, or searched, so as to allow scientists to piece together a picture of greater experimental clarity.

The present invention provides the extraction of wild jujube seed oil, the preparation of soft capsule and the function on central nervous system. The extraction of wild jujube seed oil includes squeezing wild jujube seed in squeezer to obtain dregs and coarse oil; mixing the dregs and petroleum ether to obtain refined oil after recovering petroleum ether; dissolving the coarse oil and petroleum ether to obtain refined oil after recovering petroleum ether; merging refined oil, heating in evaporating pot to eliminate residual solvent and water and to obtain wild jujube seed oil. The preparation of soft wild jujube seed oil capsule includes soaking gelatin, heating in water bath, adding glycerin and Nipagin A, and filtering to obtain gelatin liquid; preparing gelatin plate with the gelatin liquid; sandwiching wild jujube seed oil with two gelatin plates and pressing with steel mold. Research shows that wild jujube seed oil has tranquilizing, hypnotic, memory-promoting and anxiety resisting effects, and may be used in treating neurosis and insomnia.

The present invention relates to a supercritical CO2 extruction technique of nitraria sibiria seed oil. Said invention is characterized by that after the dried nitraria sibiria seed is pulverized, the pulverized dried nitraria sibiria seed can be placed in the supercritical extraction equipment, and extracted, its extraction pressure is 15-45 MPa, extraction temperature is 25-50 deg.C, CO2 flow rate is 45-250 L/h and extraction time is 1-8 hr. Said invention also provides the application of said nitraria sibiria seed oil in the preparation of medicine, for example capsule preparation with the function of reducing blood fat.

The present invention relates generally to a compartmentalized nerve culture system. The compartmentalized nerve culture system has numerous applications including isolation of axons and cell bodies. The present invention has broad application for basic and pre-clinical research including, but not limited to, use in neuroscience, neuronal culture systems, co-culture systems, drug screening, morphological studies, and toxicology studies.

The present invention describes a dissociated cell culture system comprising cells of the hippocampus, one of the brain areas affected by Alzheimer's Disease (AD) or amyloid beta-related diseases. This culture system comprises hippocampal neuronal and glial cells from animal models of AD, particularly, but not limited to, double transgenic mice expressing both the human APP mutation (K670N:M671L) (mAPP), and the human PS1 mutation (M146L) (mPS1), and serves as a powerful tool for the screening and testing of compounds and substances, e.g., drugs, for their ability to affect, treat, or prevent AD or β-amyloid-related diseases. The effects of a test substance on the cells in this culture system can be quantitatively assessed to determine if the test substance affects the cells biochemically and/or electrophysiologically, and/or optically, and/or immunocytochemically. The present in vitro culture system is advantageous for AD drug screening, because it is rapid and efficient. By contrast, even in the fastest animal model of AD, pathology does not start before the end of the second month. If such in vivo animal models are used, it is necessary to wait at least the two month time duration or longer to test for drug efficacy for AD treatment or prevention. At the same time the present invention provides a tool for production of amyloid-beta that can be used for electrophysiological, behavioral, and toxicological studies.

The invention relates to the biomedical toxicantexposure experimental equipment and the method of the experimental equipment, in particular to a gas exposure experimental device and the method. The key technology is to design and make a small sized, portable, and electric controlled dynamic gas exposure experimental device; the device comprises a miniature fan motor (3), a section of removable and movable toxicant exposure chambers (5), a set of air transmission pipelines and adjusting valves (4) connected with the fan motor and the toxicant exposure chambers, and a set of exhaust tubes (6) and collection bags for waste gas (7) combined with the toxicant exposure chambers; the remote controls for gas flow rate, flow, and exposure time are performed by the miniature adjustable fan motor and the air transmission pipelines; the animal foods and drinking water (15) are provided by the removable toxicant exposure chambers (5) to meet the requirements for long-time and repeated exposure; and the performance evaluations for filed gas exposure experiment, toxicological study and the equipment for harmful gas are performed by the different combinations and different exposure modules of the small sized movable exposure device.

Systems and methods are disclosed to objectively identify sub-second behavioral modules in the three-dimensional (3D) video data that represents the motion of a subject. Defining behavioral modules based upon structure in the 3D video data itself - rather than using a priori definitions for what should constitute a measurable unit of action - identifies a previously-unexplored sub-second regularity that defines a timescale upon which behavior is organized, yields important information about the components and structure of behavior, offers insight into the nature of behavioral change in the subject, and enables objective discovery of subtle alterations in patterned action. The systems and methods of the invention can be applied to drug or gene therapy classification, drug or gene therapy screening, disease study including early detection of the onset of a disease, toxicology research, side-effect study, learning and memory process study, anxiety study, and analysis in consumer behavior.

The invention belongs to the analytical and testing instrument field and relates to a development of a grouping toxicology analyzer; the device consists of six modules of a component separating module, a component collecting module, an automatic mechanical arm, a solution processing platform, a compound measuring module and a data processing and control system, wherein, the component collecting module, the automatic mechanical arm and the solution processing platform are arranged in a superclean bench so as to keep the sterile environment; the device is a new analytical system, can complete the relative complex work of three parts of sample application of sample separation, component analysis and bioactivity analysis with high flux on line, thus reducing the dependence on operators greatly, improving the related work efficiency and the reliability of evaluation results at the same time and having wide application prospect in the aspects of the development of toxicology of environmental pollutants, the development of compound metabolites, the toxicity evaluation of industrial products, the screening of drugs and the analysis of effective components, etc.

The present invention relates to a Chinese medicine for curing acute and chronic pharyngitis and tonsillitis and its preparation method. It is made up by using extract of Changbaishan lantern plant calyx as active component and adding borneol and other auxiliary materials through a certain preparation process. Said Chinese medicine can be made into pill preparation, powder preparation, tablet preparation, capsule preparation or oral liquor.

The invention relates to a method for constructing a heart cell system of blotchy rockcod, which comprises: taking a heart tissue of the blotchy rockcod as a material, adopting a trypsin digestion method to start primary culture, and culturing the heart tissue in a DMEM/F12 liquid medium which contains fetal calf serum, basic fibroblast growth factors, I-type insulin-like growth factors, chondroitin sulfate and culture supernatant of fin cells of the blotchy rockcod in the log phase and has a pH value of between 7.0 and 7.4; and adopting the trypsin digestion method for subculturing. The heart cell system of the blotchy rockcod constructed by the method is subcultured for 70 generations currently. The technology is scientific and reasonable, is hopeful to be applied to separation and breeding of fish viroids, preparation of virus vaccines and research in the aspects of interaction of viruses and host cells, and so on, can also be taken as a model for researching environmental pollutants in environmental toxicology, and performs pollution monitoring and safety evaluation on various environmental pollutants in the sea such as genic toxins, mutagen, carcinogens, environmental hormones and endocrine disruptors.

The invention discloses a bee glue and perilla herb oil health-care food for assisting blood fat lowering and a preparation method thereof. The method comprises the following steps of: (1) freezing bee glue in a freezing cabinet, then pulverizing and mixing; (2) putting the bee glue, perilla herb oil, corn oil and natural vitamin E into a mixer and stirring as well as pulverizing the mixed materials by using a colloid mill and cooling; (3) putting gelatin, glycerin and purified water into a sol pot, heating, stirring and vacuumizing into a glue solution state; putting into a glue solution barrel and preserving heat for later use; (4) in the presence of a specific environment condition, pelleting in a soft capsule press to obtain soft capsules; (5) cooling and shaping; and (6) washing with 95 percent alcohol until the surfaces of the soft capsules have no oil stains and then putting the soft capsules into a drying room for drying twice. The health-care food is safe and effective and has small dosage; and proved through toxicologic studies, the soft capsules for assisting blood fat lowering have safety, no toxicity, obvious effect and the function of assisting the blood fat lowering.

The invention discloses a method for screening and identifying wastewater and treating poisonous substances in water and belongs to the field of toxicological researches on environments and ecology. The method comprises the following steps of: poisonous substance characteristic analysis, poisonous substance identification, and poisonous substance confirmation. The problems that the wastewater is high in toxicity and complex in component, the composite toxic effect mechanism of poisonous substances is complex, the ecologic safety of the treated water is difficult to define by the traditional wastewater discharge standard and the chemical analysis method is complex in step, high in cost and the like are solved. The method can be used for rapidly and effectively judging the physical and chemical properties of poisonous substances existing in the wastewater and initially determining the types of the poisonous substances so as to provide an important basis for the further identification of the poisonous substances and provide an important reference basis for a removal scheme for the poisonous substances in the wastewater.

The invention discloses health-care food, which is characterized by comprising the following raw material medicaments in portion by weight: 1 to 30 portions of ageratum, 1 to 50 portions of perilla, 1 to 100 portions of honeysuckle, and 1 to 50 portions of hawthorn. The toxicological research shows that the health-care food has good safety, and the pharmacodynamical test shows that the health-care food has good functions of clearing throat and enhancing immunity.

The invention discloses a quantum-dot immunofluorescence kit for simultaneously detecting gentamycin and quinolone medicines, and at the same time, the invention also discloses a preparation method of ciprofloxacin hapten, corresponding artificial antigen and a quantum dot-labelled monoclonal antibody. The kit takes a conjugate of gentamycin semiantigen and carrier protein as well as the conjugate of the ciprofloxacin semiantigen and carrier protein as a primordial covering, and takes a mixture of a quantum dot-labelled gentamycin specific antibody and a ciprofloxacin specific antibody as a detection antibody. The preparation method is simple and feasible, has the advantages of low cost and high semiantigen yield, and the quantum-dot immunofluorescence kit has the advantages of simpleness, rapidity, large sample processing amount, high sensitivity, and strong specificity. The quantum-dot immunofluorescence kit can be used for self-control of quality and food safety supervision of a food production enterprise, and routine screening of a detection mechanism, and can be used as an auxiliary means for animal pharmacological and toxicologic researches in scientific research.

The invention relates to the field of cigarette smoke toxicology researche, in particular to a method for determining and evaluating cytotoxicity caused by cigarette smoke. The method for determining and evaluating cytotoxicity caused by cigarette smoke comprises the following steps: (1) preparing an extracting solution of cigarette smoke particulate matters; (2) culturing an HMy2.CIR human B lymph mother cell line; (3) contaminating; (4) developing; (5) calculating the cell survival rate; and (6) drawing a graph to calculate IC50. The invention has the advantages that no individual difference exists, thus influences on experiments of the individual difference can be eliminated; materials are convenient to obtain, and the quantity of cells is not limited so that the requirement on a large sample quantity can be met; ethical issues are not involved; the action time with CCK-8 is short, the developing time can be shortened, experimental errors caused by long developing time can be reduced, and an experimental process is quicker.

The invention discloses a harm reducing natural plant additive for cigarettes, and a preparation method and application thereof. The additive comprises a natural plant extract composition or a compound natural plant extract, wherein the natural plants include herba epimedii, eclipta, tussilago farfara, leonurus, dandelion and trichosanthes peel. The additive is prepared according to a method as follows: extracting and refining herba epimedii, eclipta, tussilago farfara, leonurus, dandelion and trichosanthes peel respectively, and then blending all of the extracts in a ratio of (1-8): (0.8-6): (0.5-4): (0.3-4): (0.3-5): 1, thereby forming the harm reducing additive for cigarettes; or mixing herba epimedii, eclipta, tussilago farfara, leonurus, dandelion and trichosanthes peel in a ratio of (0.5-8): (0.5-6): (0.2-4): (0.3-4): (0.3-5): 1 and then extracting and refining the mixture, thereby forming the harm reducing additive for cigarettes. The additive is added to the cut tobacco, cut stems and slices of cigarettes in an addition proportion of 0.002-0.5wt%. Compared with the prior art, proved by pharmacological and toxicological smoke study, the harm reducing natural plant additive for cigarettes has obvious biological harm reduction effect after being added to the cigarettes.

The present invention is directed to the production, breeding and use of transgenic non-human animals such as mice in which specific genes or portions of genes have been replaced by homologues from another animal to make the physiology of the animals so modified more like that of the other animal with respect to drug pharmacokinetics and metabolism. The invention also extends to the use of the genetically modified non-human animals of the invention for pharmacological and/or toxicological studies.

The invention provides an artificial feed for scotogramma trifolii. The artificial feed for the scotogramma trifolii comprises the following components by weight percentage: 0.8-1.2 percent of agar, 0.06-0.09 percent of sorbic acid, 1.8-2.8 percent of beer yeast, 5.0-7.5 percent of soybean meal, 5.0-7.5 percent of corn flour, 14-17 percent of tomato sauce, 0.07-0.09 percent of compound vitamin B,0.2-0.3 percent of ascorbic acid (Victoria C), 0.1-0.2 percent of ethyl p-hydroxybenzoate, 0.2-0.3 percent of propionic acid and 64-71 percent of water. The invention also provides a preparation method and applications of the artificial feed. The preparation method of the artificial feed is low in cost, easy to operate and high in practicality; the artificial feed provides necessary conditions for scale production of the scotogramma trifolii, relieves the pollution caused by feeding with a natural feed and solves the anniversary and subculture feeding problems of the scotogramma trifolii; anda foundation for providing insect sources with the same physiological indexes for a further biological, genetic and toxicological research is laid.

The invention discloses a method for detecting nanoscale plastic particles in an animal biological sample, and relates to the field of biological monitoring by utilizing the animal biological sample and toxicological research of tissues and organs. The method comprises the following steps: collecting biological samples such as animal marine products, rodent experimental animal organs and the like,digesting and releasing plastic particles in the samples through alkali liquor, carrying out surface modification on the nano-plastic by utilizing the properties of the digested product, and extracting the nano-plastic by utilizing the effects of solid-phase adsorbent adsorption, coating coagulating sedimentation and the like; and finally, detecting the concentration of the plastic specific indication compound by using a Pyr-GC-MS combined platform, and converting the mass concentration of the indication compound into the mass of the nano plastic. The problems caused by different filter membrane apertures, filter membrane blockage and the like when plastic in a tissue sample is extracted through conventional membrane filtration are solved. A trace and ultra-trace pollution analysis meansis provided for research on health problems caused by nano-plastics and exposure risks of people. The method is simple, low in cost, high in benefit and wide in application range.

The invention provides a processing method and a processing device for efficacy of combined medicine. The processing method comprises: obtaining a dose-effect curved band of expected additive effect of combined medicine; obtaining an actual dose-effect relationship curve formed by variation of actual effect value of the combined medicine with variation of dosage of a certain target component medicine in the combined medicine; comparing position relationships of the actual dose-effect relationship curve and the dose-effect curved band, and when the actual dose-effect relationship curve is above the dose-effect curved band, efficacy output of the combined medicine being synergy; when the actual dose-effect relationship curve is below the dose-effect curved band, efficacy output of the combined medicine being antagonism; and when the actual dose-effect relationship curve is in the dose-effect curved band range, the efficacy output of the combined medicine being addition. The processing method solves a problem in the prior art that efficacy cannot be accurately detected when multiple medicines are used in a combined manner. The method can be widely applied in research and development of compound medicines, toxicologic study, efficacy and safety evaluation of the combined medicine, and environmental evaluation.

The invention provides a culture medium and method for inducing human pluripotent stem cells to be differentiated into alveolar cells or alveolar organs, and particularly relates to a culture medium and method for inducing the human pluripotent stem cells to be differentiated into lung precursor cells or alveolar cells by using a small molecule activator/inhibitor. The cells obtained by induced differentiation express typical lung precursor cells and alveolar cell biomarkers, and a three-dimensional human alveolar organ has an alveolar-like blister structure and can be used as a material for toxicological research, drug screening and lung disease research.

A Chinese medicine for treating the general debility is prepared from 12 Chinese-medicinal materials including ginseng, astragalus root, Chinese angelica root, Chuan-xiong rhizome, etc.