Harm reducing natural plant additive for cigarettes, and preparation method and application thereof
A technology of natural plants and additives, applied in the fields of application, tobacco, and tobacco treatment, etc., to achieve the effect of clear biological harm reduction
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[0028] 1. Cytotoxicity evaluation of tobacco smoke added with natural plant tobacco harm reduction additives
[0029] 1. Purpose of the test
[0030] The cytotoxicity of cigarettes with different treatments was evaluated.
[0031] 2. Test samples
[0032] There are 2 types of cigarettes, labeled as control cigarettes and experimental cigarettes (natural plant tobacco with harm-reducing additives added, the addition ratio is 0.03%).
[0033] 3. Experimental plan
[0034] 3.1 Experimental cells: human bronchial epithelial cells (BEP2D cells) immortalized by adenovirus-12 / SV40 virus, the cells use LHC-8 serum-free medium (purchased from Biofluid, USA) at 37°C, 5% CO2 and 95% humidity cultivated under conditions.
[0035] 3.2 Preparation and poisoning of cigarette smoke condensate (CSC)
[0036] Collect cigarette smoke according to standard smoking conditions (standard conditions defined by smoking machines for routine analysis in GB / T16450-1996), prepare smoke condensate (CS...
Embodiment 1
[0089] Take Epimedium, Eclipta, Coltsfoot, Leonurus, Dandelion and Trichosanthes bark respectively, grind them and mix them uniformly according to the ratio of 0.5:0.5:0.2:0.3:0.3:1, add 8 times the amount of pure water, soak for 2 hours, Heat reflux extraction at 75°C for 1 hour, extract 3 times in total, combine the extracts from the 3 times, concentrate at 80°C, and pressure 0.02Mpa to a fluid extract with a density of 1.12. Add 2 times the volume of ethanol, let it stand for 24 hours, filter the precipitate, concentrate to a density of 1.12 liquid extract under the conditions of 80°C and a pressure of 0.02Mpa, and filter to obtain the harm-reducing additive for tobacco. Take 10g of the additive, dissolve it in 20kg of distilled water, spray it on 500kg of shredded tobacco, and roll it into cigarettes.
Embodiment 2
[0091] Take Epimedium, Eclipta, Coltsfoot, Motherwort, Dandelion and Trichosanthes bark respectively, grind them and mix them evenly according to the ratio of 1:0.8:0.5:0.5:0.5:1, add 6 times the amount of pure water, soak for 6 hours, Heat reflux extraction at 70°C for 1.5h, extract 3 times in total, combine the three extracts, concentrate at 75°C and pressure 0.06Mpa to a fluid extract with a density of 1.11. Add 2 times the volume of ethanol, let it stand for 36 hours, filter the precipitate, concentrate at 75°C and a pressure of 0.06Mpa to a liquid extract with a density of 1.11, and filter to obtain the harm-reducing additive for tobacco. Take 50g of the additive, dissolve it in 20kg of distilled water, spray it on 500kg of shredded tobacco, and roll it into cigarettes.
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