16 results about "Routine analysis" patented technology

The present invention discloses a method for detecting Hg with an "off-on" type fluorescence sensor. 2+ The method for detecting Hg in carbon dots is a method for detecting Hg in carbon dots. The method includes: 1) preparing carbon dots with polyethyleneimine and ammonium citrate as precursors; 2) detecting Hg in carbon dots. 2+ The addition of Hg 2+ The standard curve of Hg concentration and fluorescence quenching degree was obtained and the Hg 2+ 3) Glutathione was mixed with serum and then added to carbon dots and Hg 2+ In the system, a standard curve of glutathione concentration and fluorescence recovery degree was constructed to obtain the concentration of glutathione in serum samples, with a detection limit as low as 61.89 nmol / L. This invention is the first to use an "off-on" type fluorescence sensor to detect Hg 2+ It can quantitatively detect two substances, GSH and glutathione, with simple operation, safe and efficient, and suitable for routine analysis.

The invention relates to the technical field of network security, in particular to an attack identification method and device, electronic equipment and a storage medium, which are used for timely and effectively discovering attacks destroying DNS load balance. The method comprises the steps that a detection routine analyzes a DNS event message, generates a detection DNS request and sends the detection DNS request to an NS server, the DNS event message is generated when a DNS analyzer determines that the NS server does not respond to an original DNS request, the original DNS request comprises a request domain name, and the DNS event message comprises the request domain name and an NS request type; based on the response state of the NS server to the detection DNS request, a detection result is determined, and the detection result represents whether the analysis service of the NS server fails or not and whether the request domain name has attack characteristics or not.

The invention discloses a method for determining the content of diquafosol tetrasodium and detecting related substances, wherein the method mainly adopts high performance liquid chromatography to determine and inspect the content of diquafosol tetrasodium raw material medicine and its related substances. The method is time-saving and labor-saving, has high precision, accurate content determination results, good repeatability and recovery rate, and the method is verified and can be used for routine analysis and quality control of diquafosol tetrasodium raw material medicine and preparation samples.

The invention discloses an HPLC (high performance liquid chromatography) detection method of diltiazem hydrochloride and an isomer thereof, effective separation of diltiazem hydrochloride and the diltiazem hydrochloride isomer can be realized, accurate nature determination can be realized, the method is simple and easy to implement, an instrument is a common high performance liquid chromatograph, the detection cost is low, and the method is suitable for industrial production. The method is good in specificity, linearity, precision, stability and other tests, can be used for conventional analysis and quality control of diltiazem hydrochloride and diltiazem hydrochloride isomers, and meets the requirements of research, development and production.

The invention provides a method for establishing a mathematical model of a suction curve of a smoking machine for conventional analysis and the mathematical model, and the method comprises the following steps: firstly, according to the geometrical relationship of a suction mechanism, establishing geometrical relationships between a piston movement distance x and a connecting rod length l, a crankshaft radius r, a distance h between a fulcrum and a crankshaft center, and a crank angular displacement theta; according to the principle of piston movement, an expression of the suction flow phi based on the geometrical relationship of the suction mechanism is established; simplifying and unifying the expression of the suction flow phi; and finally, converting the expression of the suction flow phi according to the corresponding relation of the piston motion, the ventilation capacity and the suction duration to obtain a mathematical model. According to the method and the obtained mathematical model, the suction curve of the smoking machine for conventional analysis is summarized, and a unified suction curve mathematical model is formed, so that a research basis is provided for research and design of a smoking mechanism.

This invention discloses a sensitive fluorescence sensor for detecting NO in ham sausage samples. 2‑ The method belongs to the field of analytical detection. The steps are as follows: 1. Prepare carbon dots using citric acid and cauline as precursors; 2. NO in the carbon dots 2‑ The addition of [a specific substance] will quench fluorescence through dynamic quenching and diazotization effects, based on which NO [a specific substance] can be constructed. 2‑ Standard curves of NO concentration and fluorescence quenching degree were obtained, and the NO concentration in ham sausage samples was determined. 2‑ The concentration of NO. This invention is the first to use a sensitive fluorescence sensor to sequentially detect the concentration of NO. 2‑ It enables quantitative detection of substances and is simple, safe, and efficient, making it suitable for routine analysis.

This application relates to the technical field of hypoxia tolerance of grass carp populations, and specifically relates to a method, device, equipment and medium for identifying the hypoxia tolerance ability of grass carp populations. This application obtains the blood routine information of the grass carp population to be tested; obtains a hypoxia tolerance screening model, and the hypoxia tolerance screening model characterizes the relationship between the blood routine information and the hypoxia tolerance ability of the grass carp population; and obtains a hypoxia tolerance identification result according to the blood routine information and the hypoxia tolerance screening model. The technical solution of this application can judge the hypoxia tolerance ability of grass carp only by collecting the blood of grass carp for blood routine analysis to obtain blood routine information.

The invention discloses a fast protein electrophoresis buffer solution dry powder and preparation and application thereof, the buffer solution dry powder takes prepared 1L of a 1 * buffer solution as a reference standard and comprises the following components by mass: 3-6g of tris (hydroxymethyl) aminomethane, 15-25g of glycine, 4-8g of N-(2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid and 0.5-1g of lithium dodecyl sulfate, and the water content of the dry powder is less than or equal to 3% (w / w). According to the buffer solution dry powder disclosed by the invention, by optimizing core components and proportions of a buffer system, the protein electrophoresis buffer solution dry powder which is rapid in electrophoretic separation, high in resolution, low in toxicity, stable and simple and convenient to prepare is realized, and the protein electrophoresis buffer solution dry powder is suitable for mainstream protein electrophoresis scenes such as SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) and is particularly suitable for high-throughput detection, clinical rapid diagnosis and laboratory conventional analysis.

The application discloses a method for visual detection of uric acid based on fluorescent carbon dots, and belongs to the field of analysis and detection. The carbon dots are prepared by taking 3-amino phenyl boronic acid and L-glutamine as raw materials, and anhydrous ethanol and deionized water as solvents. The imino group in uric acid has a positive charge, while the boronic acid group on the surface of the carbon dots after doping treatment has a negative charge. There is an electrostatic interaction between the two and a complex is formed, so that the fluorescence is quenched, and in this process, the fluorescence color also changes. Based on this, a relationship curve between the uric acid concentration and the fluorescence quenching degree is constructed, the detection limit is as low as 33.0nM, and the method is applied to visual detection of uric acid in water samples, serum and urine, visual detection of uric acid is realized, and the detection limits are 33.0nM, 37.6nM and 72.0nM respectively. The method is simple, safe and efficient, and is suitable for routine analysis.

The invention discloses an online ratio analyzer sampling probe back flushing method, and belongs to the field of sulfur recovery pipeline sampling probe blockage treatment.The online ratio analyzer sampling probe back flushing method comprises the following steps that when back flushing needs to be started, the blockage degree of a sampling probe is obtained; and the reverse blowing controller starts a reverse blowing electromagnetic valve and controls pulse reverse blowing according to the blockage degree to perform reverse blowing according to different reverse blowing periods and reverse blowing flows, so that impurities on the sampling probe are blown back into the sulfur recovery pipeline. When the sampling probe needs to be subjected to back flushing, the back flushing controller sets the corresponding back flushing flow and back flushing period according to different blocking degrees, so that compared with the existing mode of not distinguishing the blocking degrees, the back flushing time is saved, the efficiency of eliminating the blocking obstacles is improved, and the conventional analysis and measurement of the gas sample by the analyzer are ensured.

The present invention discloses a method for detecting tetracycline antibiotics in milk based on deep ultraviolet emission carbon dots, and belongs to the field of analysis and detection. The method for detecting tetracycline antibiotics in milk based on deep ultraviolet emission carbon dots comprises the following steps: (1) preparing a substance rich in hydroxyl, amino and carboxyl groups into a carbon dot solution; (2) performing a simple pretreatment after mixing milk with tetracycline, and then mixing with the carbon dots in (1), and obtaining the degree of fluorescence quenching of the mixed solution by fluorescence spectrum detection, and finally constructing a standard curve of tetracycline concentration and the degree of fluorescence intensity quenching of the mixed solution; (3) according to the standard curve of step (2), the purpose of detecting tetracycline antibiotics in milk samples is achieved. The present invention uses deep ultraviolet emission carbon dots as fluorescent probes for the first time to realize the quantitative detection of tetracycline antibiotics in milk, and has the advantages of high sensitivity, simple operation, safety, high energy utilization and low cost, and is suitable for routine analysis.

The invention relates to the technical field of blood analysis, in particular to an animal blood routine analyzer which comprises an analyzer body, a sampling part is arranged on the analyzer body, a fixed bottom plate is fixedly connected to the inner wall of the sampling part, and a rotary shaking mechanism, an up-down shaking mechanism and an adsorption stabilizing mechanism are arranged at the top end of the fixed bottom plate; the rotary shaking mechanism comprises a sliding block plate, a shaking plate and a placing column, a placing groove is formed in the top end of the placing column, the bottom end of the placing groove is semicircular, a circular placing hole is formed in the sliding block plate in a penetrating mode, and the circular placing hole and the placing groove are used for placing a glass test tube and conducting preliminary limiting; according to the invention, uniform distribution of the anticoagulant in a blood sample is ensured through a dual oscillation mechanism, and formation of tiny clots is effectively prevented, so that the risk of blockage of a sampling needle, a pipeline and a detection cavity of an analytical instrument is avoided, and smooth operation of the instrument and timeliness of a detection process are guaranteed.

The assessment of confirmation therapeutic protein identification and purity can be assessed through comparative analysis against a reference standard. Therapeutic protein identification is not only dependent on the amino acid sequence, its secondary structure, but also the post-translational modifications that it possesses and all impact its stability. The current methods are labor intensive and require extensive sample preparation. The proposed method employs infrared microscopy to acquire hyperspectral images of a fixed sample volume of only 2 μL of the intact protein in solution. The spectral data obtained from these images are then analyzed by correlation algorithms, specifically: two trace in two-dimensions (2T2D) and two-dimensional correlation spectroscopy (2D-COS) providing both a simple output for interpretation based on the 2T2D, but also the dynamic fingerprint of the desired therapeutic protein using the 2D-COS, the combination of which provide the elements for decision making. These correlation algorithms also provide enhanced spectral resolution which is key to the analysis. The effort is valid for both the initial evaluation of characterization and for follow-on assessment through the drug life cycle. A rapid, robust and reproducible method for routine analysis is proposed in this application.

The invention relates to the technical field of hypoxia resistance of grass carp groups, in particular to a method, a device, equipment and a medium for identifying hypoxia resistance of grass carp groups. The method comprises the following steps: acquiring blood routine information of a to-be-detected grass carp population; obtaining a hypoxia-resistant screening model, wherein the hypoxia-resistant screening model represents the relationship between the blood routine information and the hypoxia-resistant capability of the grass carp population; and according to the blood routine information and the hypoxia-resistant screening model, obtaining a hypoxia-resistant identification result. According to the technical scheme, the hypoxia tolerance of the grass carp can be judged only by collecting the blood of the grass carp and carrying out blood routine analysis to obtain the blood routine information.

This invention relates to a pretreatment method for conventional chemical analysis of tobacco, comprising the following specific steps: S1, quantitative feeding: conveying and removing caps, adding powder, adding liquid, and resetting; S2, oscillating extraction; S3, filtration separation: needle assembly, secondary cap removal, liquid extraction, secondary capping, and filtration; S4, removal and resetting; wherein quantitative feeding is achieved through the cooperation of separate rotating gripper assemblies and clamping assemblies, which not only avoid mutual interference but also ensure accuracy through coordinated positioning; during filtration separation, the synchronously staggered rotating gripper assemblies and liquid extraction assemblies effectively avoid interference between steps, reduce the use of conveying mechanisms, and shorten the operation distance. This invention, without requiring excessive conveying mechanisms, makes each processing step simple and clear through the corresponding correlation and positioning between steps, resulting in more compact connections between adjacent steps and facilitating subsequent rapid cleaning.

The application discloses a method for detecting ascorbic acid in urine based on nitrogen and boron co-doped fluorescent carbon dots, and belongs to the field of analysis and detection. The carbon dots are prepared by taking citric acid, ethylenediamine and boric acid as precursors, and the fluorescent quantum yield of the carbon dots reaches 84.5%. The high iodine oxide oxidizes the surface functional groups of the carbon dots to generate non-fluorescent groups, so that the fluorescence of the carbon dots is quenched. The ascorbic acid preferentially reacts with the high iodine oxide in an oxidation-reduction reaction, thereby inhibiting the fluorescence quenching effect of the high iodine oxide, and the inhibition degree is related to the concentration of the ascorbic acid. Based on this, a relationship curve between the concentration of the ascorbic acid and the degree of inhibition of fluorescence quenching is constructed, so that the quantitative detection of the concentration of the ascorbic acid in urine is realized, and the detection limit is as low as 0.01 nmol / L. The application first uses an "inhibition" type fluorescence sensor to quantitatively detect the ascorbic acid, and is simple, safe, efficient and suitable for routine analysis.