The present invention discloses a method for preparing a high-sensitivity nano-zirconia-doped pafenolol molecular imprinting electrochemical sensor, the high-sensitivity nano-zirconia-doped pafenolol molecular imprinting electrochemical sensor is prepared by use of pafenolol as a template molecule, (5s, 8s)-3-(4'-chloro-3'-fluoro-4-vinyl-biphenyl-3-yl)-4-hydroxy-8-ethyoxyl-1-aza-spiro [4.5] dec-3-ene-2-one as a single function monomer, azobisisobutyronitrile as an initiator, nano-zirconia as a dopant and maleic rosin ethylene glycol acrylate as a crosslinking agent, wherein the maleic rosin ethylene glycol acrylate is prepared from rosin as a raw material, and the method is simple and practical, and overcomes the shortcomings that a conventional analysis method is complicated, expensive in equipment, and low in sensitivity.