This invention relates to the field of detection and analysis technology, and discloses a method for detecting
perfluorooctanoic acid (PFOA) in water. The method includes the following steps: filtering a
water sample through a filter membrane and collecting the filtrate; using a C18 reversed-phase
chromatographic column, with an
aqueous solution containing tetrabutylammonium bisulfate as mobile phase A and
acetonitrile as mobile phase B,
isocratic elution is performed at a detection
wavelength of 208-215 nm, and detection is performed using a
UV detector or a
diode array
detector;
qualitative analysis is performed based on chromatographic
retention time, and quantitative analysis is performed using the external standard method based on chromatographic
peak area. This invention, by optimizing the detection
wavelength and introducing tetrabutylammonium bisulfate as an
ion-
pairing reagent, effectively improves the chromatographic peak shape and resolution of PFOA, achieving a
correlation coefficient R0 within a wide
linear range of 0.5-200 mg / L. 2 The
detection limit is >0.999, which meets the detection requirements of most water bodies; the method has good
repeatability, with a spiked
recovery rate of up to 98.30%-108.35% in complex matrices; it has strong anti-interference ability, and the detection results are stable in the
pH range of 4-10, making it suitable for actual water samples under different acid and alkaline conditions.