Bifidobacterium bifidum CCFM1063 and application thereof
A technology of Bifidobacterium bifidum and functional bacterial agent, applied in the field of microorganisms, can solve the problems of increased risk of death of type 2 diabetes, and achieve the effects of relieving PFOA toxicity, relieving colitis, and improving the expression of MafA gene
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Embodiment 1
[0090] Example 1: Bifidobacterium bifidum CCFM1063 has good tolerance to simulated gastrointestinal fluid
[0091] The cryopreserved Bifidobacterium bifidum CCFM1063 was inoculated in mMRS medium (MRS medium + 0.05% cysteine hydrochloride), cultured anaerobically at 37°C for 48 hours, and then subcultured in mMRS medium for 2-2 hours. After 3 times, take 1 mL of the culture solution of Bifidobacterium bifidum CCFM1063, mix it with 9.0 mL of pH 2.5 artificial simulated gastric juice (containing 1% pepsin, mMRS medium at pH=2.5), and culture it anaerobically at 37°C. Samples were taken at 0h, 0.5h, 1h, and 2h, and the mMRS agar medium was used to pour culture to count plate colonies, determine the number of viable bacteria and calculate their survival rate.
[0092] The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria at the 0th hour in the culture solution, ex...
Embodiment 2
[0098] Example 2: Bifidobacterium bifidum CCFM1063 has no toxic side effects on C57BL / 6J mice
[0099]Bifidobacterium bifidum CCFM1063 cells were resuspended in 3% sucrose solution to make a concentration of 3.0×10 9 CFU / mL bacterial suspension. Eight healthy male C57BL / 6J mice with a body weight of about 16-20 g were taken. After one week of adaptation to the environment, the bacterial suspension of this concentration was administered orally once a day, observed for one week, and the death and body weight were recorded.
[0100] The results of these tests are listed in Table 3. These results indicate that feeding concentrations of 3.0×10 9 CFU / mL of Bifidobacterium bifidum CCFM1063 had no significant effect on mice, no significant change in body weight, and no death. The appearance of the mice had no obvious pathological symptoms.
[0101] Table 3 Changes in body weight and death of mice
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[0103] Note: -: no death of mice
Embodiment 3
[0104] Example 3: Bifidobacterium bifidum CCFM1063 has a recovery effect on intestinal flora imbalance in type 2 diabetic mice
[0105] Take 40 healthy male C57BL / 6J mice weighing 16-20g, adapt to the environment for 1 week, and divide them into 5 groups randomly: blank control group (NC), model control group (M), rosiglitazone control group (RH) , Bifidobacterium bifidum CCFM1063 intervention group (CCFM1063), Bifidobacterium bifidum BB12 control group (BB12) each group contains 8 mice, and the dosage of gavage bacterial suspension is 3.0×10 9 CFU / mL, resuspended in 3% sucrose solution. The grouping and treatment methods of experimental animals are shown in Table 4:
[0106] Table 4 Grouping of experimental animals
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[0109] Week 2-7: The mice in the normal group were fed with normal feed, and the rest of the mice were fed with high-fat feed.
[0110] On the 1st day of the 11th week, all mice were fasted for 12 hours, the normal group was injected wi...
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