The invention discloses aqueous anti-graffiti adhesion resistant paint and a preparation method thereof, and belongs to paint and a preparation method thereof. The paint consists of the following components by weight portion: 50 to 55 portions of emulsion, 2 to 4 portions of organic silicon emulsion, 14 to 16 portions of siloxane emulsion containing hydroxy functional group, 3 to 5 portions of hydrophobic organic silicon modified emulsion, 0.15 to 0.2 portion of smooth flow agent, 0.85 to 0.95 portion of film forming assistant, 0.1 to 0.2 portion of preservative, 0.1 to 0.2 portion of mildew preventive, 0.8 to 1 portion of ethylene glycol, 5 to 7 portions of hydrophobic agent, 0.1 to 0.2 portion of thickening agent, and 23 to 25 portions of deionized water. The invention also discloses the preparation method thereof. The paint has the advantages of good anti-graffiti adhesion resistant function, super-strong scrubbing resistance, easy cleaning, and quick and convenient construction, has zero formaldehyde, benzene and heavy metals, has low VOC, and is typical environment-friendly paint.