886results about How to "Fast detection method" patented technology

The invention relates to a method for detecting tumour cells by adopting a microelectrode array impedance biosensor chip. The method comprises the steps of: adopting nanometer immunity magnetic microballoon concentration separating technology to capture tumour cells from sputamentum or blood samples to form magnetic miroballoon-tumour cell biological magnetic combination; and utilizing magnetic force generated by a magnet to lead the magnetic miroballoon-tumour cell biological magnetic combination to be instantly absorbed on the surface of the microelectrode array impedance biosensor chip, arousing principles of changing impedance characteristic of micro-sensing electrode and adopting an impedance analyzer to conduct parallel scanning testing to the impedance characteristic of the micro-sensing electrode so as to determine whether the tumour cells exist. The invention provides a non-damage, quick, simple and convenient tumour cell detection method, and can be applied to the tumour early diagnosis and treatment, and can be used for diagnosing and monitoring the shifting and recurring of cancer cells of clinical tumour patients and carrying out wide prediction for major diseases, and also can be applied to the fields such as food detection, drug screening and the like.

Disclosed is a reagent for quantitative detection of a Beta-receptor stimulant through Europium chelate latex time-resolved immunochromatographic assay. The reagent includes test card or test strip which includes a nitrocellulose membrane, absorbent paper, a sample pad and a PVC bottom plate, and a microporous container. A test sample firstly dissolves out Eu3+ fluorescent latex particles which are attached in the microporous container and marked with an anti-Beta-receptor-stimulant small-molecule antibody and after sufficient mixing, the test sample reacts completely with the marker and then a reaction liquid is dropped to the test card or test strip to carry out immunochromatography and at the same time, an immune competition reaction with a Beta-receptor-stimulant small molecule and BSA conjugate which envelopes the nitrocellulose membrane is carried out and five minutes later, the test card or the test strip is inserted into a fluorescent reading meter to measure a fluorescent value so as o obtain a test result. The method is high in sensitivity and quantitative and integrates the advantages of simple and convenient operation and rapidness, the method is applied to rapid detection of veterinary drug residuals such as the Beta-receptor stimulant and the like in food and raw materials on sites of production fields such as plantation, cultivation, animal husbandry and food processing and the like.

The invention relates to an antibody against fucosylated protein GP73, a method for preparing the antibody against the fucosylated protein GP73, a reagent for diagnosing liver cancers, a kit for diagnosing the liver cancers, application of the reagent and / or the kit to preparing products for diagnosing liver diseases and a method for preparing purified fucosylated protein GP73. Occurrence, development and metastasis of liver cancers are diagnosed by detecting the fucosylated protein GP73 or the antibody. The antibody against the fucosylated protein GP73 can be more widely used for affinity chromatography, cDNA library screening, immunologic diagnosis or pharmaceutical preparation.

The invention discloses a method for using an electronic nose to detect the freshness of chicken. The invention aims at providing a method which uses the electronic nose to detect the freshness of chicken, has the advantages of rapidity and simpleness, easy use and objective and reliable detection result, and can reduce the impact of storing conditions to the detection result. The method comprises the following steps: a chicken sample to be detected is put into a sealed container and then heated in water bath with thermostatic control; the heated chicken sample is put into a test room, and then smell sent by the sample is detected by a gas sensor array which consists of four gas sensors of different types; the data which is aquired by the gas sensor array is collected and processed; and then the freshness of chicken is judged according to the processing result of the data. The detection method uses an electronic nose which consists of four gas sensors of different types to detect the chicken sample, and has the advantages of objective, accurate, and fast detection result, good repeatability, simple analysis process and small using amount of sensors, and reduces the extraction of eigenvalues.

The invention relates to a detecting device, which is suitable for detecting the angle of a light absorption axis of a first polarizer of a liquid crystal panel, and the liquid crystal panel comprises a first baseplate, a second baseplate and a liquid layer. The detecting device includes a light source, a rotatable detecting polarizer and a light detector. The light source is arranged at one side of the liquid crystal panel, and the liquid crystal panel is arranged on the optical path of the light source. The rotatable detecting polarizer is arranged at the other side of the liquid crystal panel and is arranged on the optical path of the light source, and the first polarizer is arranged between the baseplate and the rotatable detecting polarizer. The optical detector is arranged on the optical path of the light source, and the optical detector and the first polarizer are respectively arranged at two sides of the rotatable detecting polarizer; so using the detecting device can rapidly detect the angle of the light absorption axis of the first polarizer.

The utility model relates to a biosensor, which utilizes the specific identification of the aptamer on target biomolecule and the changes in the electric conductance property of organic productive polymerization caused by the aptamer to do detection on the biological target quantitatively. The microelectrode of the biosensor comprises a working electrode and a counter electrode; wherein, the working electrode or the counter electrode respectively comprises a plurality of electrode wires, which are the same in number and in relative array, an in situ synthesized aptamer decorated organic conductive polymerization film or nano-wire is connected between the electrode wires of the working electrode and the electrode wires of the counter electrode, and the working electrode and the counter electrode are connected with a conductor respectively. By detecting changes in the electric conductance property of the organic productive polymerization caused by the specific combination of the target biomolecule and the aptamer on the organic conductive polymerization which is fixed between the microelectrode pairs, the utility model gets the quantitative response to the bio-macromolecules, such as the target protein, directly and fast, and avoids the complex and time consuming marking and incubation in the traditional method.

The invention relates to a burst traffic detection method based on dynamic threshold. By means of the method, the burst traffic in a high-speed network environment can be effectively detected on line in real time, and the problem that a fixed threshold method cannot well adapt to the dynamically changing network environment is solved. According to the technical scheme, a threshold is dynamically adjusted according to the source IP entropy value of the normal historical traffic, a sliding window mechanism is introduced, and burst traffic is detected by comparing whether the difference value between the current detection value and the average entropy value of a sliding window exceeds the dynamic threshold; the method comprises steps as follows: generation of an initial sliding window, generation of a reference value, generation of the current detection value, abnormality judgment of the current detection value, processing of normal traffic and processing of burst traffic. The detection method is rapid, simple, high in detection rate and low in false rate, the network safety is effectively guaranteed, and the detection method has high practicability and huge economic and social benefits.

The invention belongs to the technical field of marine organism pathogen detection, and in particular relates to a multiple-PCR detection method and a kit for simultaneously detecting WSSV (white spot syndrome virus), AHPND (acute hepatopancreatic necrosis disease), EHP (enterocytozoon hepatopenaei) and IHHNV (infectious hypodermal and hepatopancreatic necrosis virus) in prawns. With the application of the multiple-PCR detection method, amplification of four pathogen DNAs can be simultaneously conducted in one time by virtue of special detection primers, namely WSSV F and WSSV R, AHPND F and AHPND R, EHP F and EHP R as well as IHHNV F and IHHNV R of the WSSV, the AHPND, the EHP and the IHHNV. The multiple-PCR detection method provided by the invention is convenient and rapid, low in detection limit, strong in specificity, high in sensitivity and high in accuracy rate and is capable of simultaneously detecting the white spot syndrome virus, the acute hepatopancreatic necrosis disease, the enterocytozoon hepatopenaei and the infectious hypodermal and hepatopancreatic necrosis virus in the prawns.

The invention discloses a Cpf1 reagent kit for quickly detecting nucleic acid of an African swine fever virus. The Cpf1 reagent kit comprises a Cpf1 detection system suitable for quickly detecting theAfrican swine fever virus, and an immune colloidal gold test strip, wherein the Cpf1 detection system comprises specific crRNA protein, specific Cpf1 protein and a single-chain DNA(ssDNA) reporting system in accordance with a p72 gene of the African swine fever virus, the specific crRNA is one or more of crRNAs from ASFV P72 crRNA1 to ASFV P72 crRNA10, and the sequence of the specific crRNA is SEQ NO.4 to SEQ NO.13; and the single-chain DNA(ssDNA) reporting system comprises ssDNA FQreporter for fluorescence detection of a microplate reader and / or ssDNA DB reporter for detecting the immune colloidal gold test strip. According to the Cpf1 reagent kit disclosed by the invention, for the first time, the Cpf1 is used for detecting the African swine fever virus, and has the advantages of beinghigh in sensitivity, high in specificity, short in time consumption, high in flux, independent of large-scale experiment equipment and the like. The advantages enable a detection method based on the immune colloidal gold test strip developed by the invention to be conveniently used in basic laboratories and breeding enterprises to be used for performing detection, identification and diagnosis on basic quick detection of the African swine fever.

The invention discloses a fluorescence quantitative PCR detection reagent for African swine fever virus, and a preparation method and the application thereof. A set of specific primers and Taqman probes are designed and synthesized to be used for detecting ASFV P54 in relevant porcine products. A standard curve drawn in the invention provides a standard for the quantitative detection of ASFV P54. The invention establishes a fast and simple real-time fluorescence quantitative PCR detection system with strong specificity and high flexibility. The detection time is only several hours, and the detection lower limit can be 15 copies. The invention can be applied to the diagnosis and quarantine technology towards the imported relevant porcine products at port, and the invention provides reliable and effective technical condition for the import quarantine work of the country without ASF.

The invention belongs to the technical field of agricultural product quality analysis, and particularly relates to a near-infrared detection method for the peanut quality and application. The method comprises the following steps that peanut samples are collected, physical and chemical testing is performed on the peanut samples, near-infrared scanning is performed on the peanut samples, denoising processing and preprocessing are performed on obtained light absorption values, obtained preprocessed light absorption values are analyzed, near-infrared spectrum characteristic wavelengths are obtained through screening, and a prediction model of the peanut quality is built through a stepwise regression method. According to the near-infrared detection method for the peanut quality and the application, the obtained information is intuitive and reliable, the characteristic wavelengths of the peanut quality are determined and are few in number, and the analytical method that the model is built through the characteristic wavelengths is applied, so that the model precision is improved; on the condition of the same prediction precision, the prediction speed is high; meanwhile, through the built near-infrared prediction model method for the moisture, protein, fat, total sugar and ash content of peanuts, the peanut quality can be analyzed more comprehensively, and usage and popularization are easy.

The invention relates to the technical field of biological detection, and particularly discloses a primer and probe combination for RAA-LFD detection of chicken infectious laryngotracheitis virus and application of the primer and probe combination. The primer comprises an upstream primer and a downstream primer, wherein the sequence of the upstream primer is shown as SEQ ID No. 1, and the sequence of the downstream primer is shown as SEQ ID No. 2; and the probe sequence is shown in SEQ ID No. 11. The primer and probe combination provided by the invention is used for detecting chicken infectious laryngotracheitis virus, and the corresponding RAA-LFD method is simple, rapid, convenient, reliable, strong in specificity, high in sensitivity and accuracy, and is more suitable for clinical field detection.

The invention discloses a metal surface defect image recognition non-destructive testing device and method, and relates to the field of automatic metal surface defect testing. The device comprises a mobile unit, a light sensation unit, a light source unit, an optical amplification unit, an image acquisition unit, an image analysis unit, a data storage unit and an alarm unit, wherein the light sensation unit and the light source unit ensure suitable brightness of an acquired image in a tested area, the optical amplification unit is used for optically amplifying the acquired image, the image acquisition unit acquires the image, the image analysis unit analyzes the acquired image in the tested area and judges whether defects exist or not, the data storage unit stores data needed by running ofthe device, the acquired image and analysis results, and the alarm unit gives an alarm for a tested problem area. Metal surfaces can be full-automatically tested, the tested area is large, the testing method is simple and rapid, and testing time and labor cost are reduced.

The invention belongs to the technical field of traditional Chinese medicine formula granules and particularly relates to semen armeniacae amarae formula granules as well as preparation and quality control methods thereof. The L-amygdalin and D-amygdalin ratio range considering both the curative effect and safety (low toxicity) is determined through study on the ratio of L-amygdalin to D-amygdalin in classical prescription decoction containing semen armeniacae amarae, semen armeniacae amarae formula granule extraction process parameters capable of guaranteeing the L-amygdalin and D-amygdalin ratio range are determined through process purification, high performance liquid chromatography is used for quantitative analysis in the quality control method, the amygdalin isomerization course is controlled by regulating process parameters such as decoction water amount and decoction time for semen armeniacae amarae decoction pieces during preparation, the semen armeniacae amarae formula granules meeting the quality requirement are prepared and has the same effect with classical prescriptions, and quality control of the semen armeniacae amarae formula granules is realized.

The invention discloses a characteristic wave bands based method for rapidly detecting the content of proteins in black fungi. The method comprises the following steps: 1) grinding and then sieving the black fungi; 2) acquiring the reflectance values of the black fungi respectively corresponding to the wavelength 1318nm, 1100nm, 1396nm, 2214nm, 1834nm, 1520nm and 2498nm; 3) converting the obtained reflectance values into absorbency values; 4) putting the absorbency values into the following linear equation respectively: Y=89.960X1-248.482X2+269.366X3-68.022X4+200.063X5-309.675X6+87.126X7+2.028, and obtaining the content of the proteins in the black fungi through computation, wherein in the equation, Y is the content of the proteins in the black fungi, and X1-X7 respectively represent the absorbency values of the black fungi respectively corresponding to the wavelength 1318nm, 1100nm, 1396nm, 2214nm, 1834nm, 1520nm and 2498nm. The invention also discloses a device for implementing the above method. The invention is mainly used for rapidly and accurately detecting the content of the proteins in the black fungi through the characteristic wave bands, is rapid and accurate in detection, greatly shortens the detection time, reduces the environmental pollution and lowers the detection cost.

The invention discloses a preparation method for roxburgh rose and a multi-index detection method. The roxburgh rose is a national medicinal material of Guizhou province, contains rich nutrients and has multiple pharmacological activities, and all parts of the roxburgh rose are valuable. The conventional detection method for the roxburgh rose is relatively simple, and the quality of the roxburgh rose is difficult to control. By setting control indexes capable of reflecting performance characteristics of the roxburgh rose, the provided detection method is simple, convenient, quick, and high in repeatability and stability; and the provided preparation method contributes to preserving active ingredients such as vitamin C in the roxburgh rose.

The invention relates to a transmitter fault diagnosis circuit and a method thereof. The fault diagnosis circuit comprises a sensor, a signal processing module, a standard signal input module, and a change over switch. The change over switch connects with the sensor and the signal processing module, and connects with the standard signal input module and the signal processing module simultaneously. According to the invention, a standard signal is inputted to the signal processing module through the standard signal input module, and connection between the sensor and the signal processing module or connection between the standard signal input unit and the signal processing module is switched through the change over switch. When a fault appears, the change over switch disconnects the connection between the sensor and the signal processing module, and connects the standard signal input module with the signal processing module, the standard signal is inputted to the signal processing module through the standard signal input module, according to the standard signal, the signal processing module carries out processing, followed by outputting, if an output result is normal, the sensor has a fault. Conversely, the signal processing module has a fault. The transmitter fault diagnosis circuit has the characteristics of simple structure, low cost and convenient maintenance.

The invention provides a schiff base fluorescent probe and a synthesis method and application thereof. The preparation method of the probe comprises the following steps: heating and refluxing 8-hydroxyl julolidine-9-formaldehyde and 4-phenyl-3-thiosemicarbazide which are reactants in ethanol for 20-24 hours; after reaction is finished, carrying out suction filtration; and washing to obtain yellow solid which is the schiff base fluorescent probe. The fluorescent probe can be used for detecting trace water and copper ions. Detection of the trace water is realized on the basis of a complex formed by the schiff base fluorescent probe and Al3+. Detection of the copper ions is realized by making schiff base probe into schiff base test paper. The detecting method is high in sensitivity, simple to operate, convenient, rapid and practical.

The invention discloses a rock mass real-time wave velocity measurement and quality evaluation method and belongs to the technical field of rock mass engineering. The method is characterized in that abutton of an ultrasonic wave sending device is continuously pressed at different ultrasonic wave sending positions and at different time, and the speed of ultrasonic waves propagated to each sensor each time is continuously calculated, and inversion is performed according to parameter change values such as wave speed, attenuation coefficient, waveform, frequency, frequency spectrum, amplitude andthe like to obtain physical and mechanical indexes and microstructure characteristics of the rock-soil body, such as change conditions of pores and cracks, so as to analyze the stability condition ofa mine rock body. The device is advantaged in that the device is combined with a micro-seismic system, testing can be completed only by arranging an ultrasonic sending system, operation is easy, theprice is low, and practicability is high; the ultrasonic testing technology has strong advantages in the field of rock-soil testing, ultrasonic waves have geometrical acoustics and physical acousticsat the same time, directivity of the sound waves is good, and energy is high; flexibility is high, and the test time, the test frequency, the test depth and the test position can be manually controlled.

The invention discloses a composite affinity column for purifying 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone, a preparation method and an application thereof. According to the invention, by employing 4% of cylindrical agarose gel as a solid phase carrier, agarose gel and an antibody are coupled to form an immunoadsorbent, and filling in a column to prepare the immunization affinity column. When a sample containing 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone passes through the immunization affinity column, 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone can be specifically adsorbed by the immunoadsorbent, other impurities flows out of the immunization affinity column, then methanol is used for eluting 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone from the column, so that the sample can be better purified.

The invention provides a detection method for galacto-oligosaccharide based on biological enzyme technology, which belongs to the technical field of food engineering. According to the invention, at first, the method of removing proteins by using trichloroacetic acid and lead acetate is employed for pretreatment of an object to be detected, and membrane filtration is utilized to remove impurities so as to obtain a sample to be detected; then, sodium hydroxide and sodium acetate with different concentration are utilized for ion exchange and gradient elution; finally, a four-potential pulsed amperometric detector is used for detection. The invention is applicable to separation and detection of cow's milk containing galacto-oligosaccharide and a variety of galacto-oligosaccharide components in a galacto-oligosaccharide solution system; employment of ion chromatography, utilization of the methods of ion exchange and gradient elution for separation of galacto-oligosaccharide and cooperativedetection with the four-potential pulsed amperometric detector enable contamination and interference to an analytical column, the detector and the like caused by macro-molecular substances such as proteins, fat and emulsifiers which might exist in the sample to be eliminated, thereby allowing accurate and rapid detection to be achieved.

A method for detecting the germination percentage of cotton seeds includes such steps as shearing a small opening at the micropylar end of seeds to expose the radicle by 2-3 mm, immersing seeds in the warm water at 53-56 deg.C until the water is cooled to 39-41 deg.C, immersing the seeds in the water at 38-42 deg.C in culture case for 25-65 min, putting the seeds on paper bed, culturing at 28-32 deg.C, examining the germination first time after the seeds are cultured for 24 hr, and examining the germination second time after the seeds are cultured for 48 hr.

The present invention relates to an immunoadsorbent and a composite affinity column for purification of fumonisin B1, aflatoxin, ochratoxin A and zearalenone. The immunoadsorbent comprises a solid carrier and fumonisin B1, aflatoxin, ochratoxin A and zearalenone monoclonal antibodies coupled with the solid carrier, the fumonisin B1 monoclonal antibody is secreted from hybridoma cell line Fm7A11 with the preservation number of CCTCCNO.C201636, the hybridoma cell line Fm7A11 is preserved in CCTCC in March 29, 2016 in Wuhan University Wuhan China. The composite affinity column is loaded with the immunoadsorbent with the fumonisin B1, aflatoxin, ochratoxin A and zearalenone monoclonal antibodies, and is used for purification of the fumonisin B1, aflatoxin, ochratoxin A and zearalenone. The composite affinity column can be used for purification and detection of fumonisin B1, aflatoxin, ochratoxin A and zearalenone toxin samples.

The invention relates to a method of detecting aflatoxin and pesticide residues in a peanut, in particular to a method of determining aflatoxin and pesticide residues in a peanut by ultra performanceliquid chromatography-quadrupole / high resolution mass spectrometry, which belongs to the field of analytical chemistry. A method of determining four kinds of aflatoxin and eleven kinds of pesticide residues in a peanut by ultra performance liquid chromatography-quadrupole / high resolution mass spectrometry adopts high performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry to determine aflatoxin and pesticide residues in a peanut, a sample is extracted through an acetic acid-acetonitrile-water extract and is purified through a PSA and C18 silica gel mixed system, an aqueous solution containing 0.1% of formic acid and methanol are used in a positive ion mode to perform gradient elution on a mobile phase, and high resolution mass spectrometry qualitative and quantitative detection is carried out. The method is accurate, efficient and reproducible, and can simultaneously determine four kinds of aflatoxin and eleven kinds of pesticide residues in a peanut.

A mark substance of specificity - anti-genicity for atrophic arthritis is carbamyl ornithine fibre connexin existied in blood plasma and joint synovium tissue of atrophic arthritis patient. The invented mark substance has excellent application value in preparing reagent and medicine for diagnosing and curing disease of atrophic arthritis.

The invention provides a method for extracting and detecting a biotin of a bird nest. The method comprises the following steps of: extracting the biotin from the bird nest by using dimethylformamide; performing centrifugal purification and concentration to prepare a sample solution; by using a one-point external standard method, developing the sample solution and a biotin standard solution on a high-performance thin-layer plate coated with silica gel 60F254 by using a developer, wherein the developer is a mixture of chloroform, acetone, methanol and glacial acetic acid; spraying 0.05 percent of potassium permanganate solution to a developed plate for coloring; and drying the developed plate, and then performing thin layer scanning with absorption wavelength of 400 nm to obtain the contentof the biotin in the bird nest. The method has the advantages of simplicity, accuracy, quickness, stability and reliability, and the defects of complicated detection steps and high time consumption of the conventional microbiological method, and high investment cost, large solvent dosage and high cost of a high performance liquid chromatography are overcome.

The invention relates to a high-efficiency and simple method for detecting a human papilloma virus (HPV) neutralizing antibody, which can be applicable to high-throughput detection of the human papilloma virus neutralizing antibody. The method is characterized by using a spot detection method for detecting cells infected by human papilloma virus or pseudotype virus. The invention further discloses applications of the method in screening and identifying the neutralized monoclonal antibody against HPV, evaluating the immunity protectiveness of HPV vaccine, the potency test (ED50 test) of HPV vaccine, etc.