35results about How to "Fast detection method" patented technology

The invention belongs to the technical field of biology, and particularly relates to a primer probe for rapid detection of hepatitis A virus RT-RPA as well as application and a rapid detection method of the primer probe. The primer probe for the hepatitis A virus RT-RPA rapid detection is shown as SEQ ID NO: 1-3, RT-RPA-LFD rapid detection of the hepatitis A virus is established, the primer probe can be used for preparing a reagent for hepatitis A virus detection, rapid, high-sensitivity, high-specificity and macroscopic high-accuracy detection of the hepatitis A virus is achieved, and an efficient and convenient solution is provided for hepatitis A virus detection.

The invention belongs to the technical field of molecular biology, and particularly relates to a pseudo-ginseng component rapid detection method based on a Proofman-LMTIA technology. According to the method, a pseudo-ginseng ITS2 sequence is taken as a specific target, and high-specificity and high-sensitivity detection of pseudo-ginseng components is realized through a designed LMTIA primer and a Proofman probe. Compared with the traditional detection technology, the invention has the advantages of simplified primer design, low-cost equipment requirement, rapid detection (only about 30 minutes) and wide application range. The method is not only suitable for detection of raw materials such as pseudo-ginseng seeds, seedlings, roots and rhizomes, decoction pieces and the like, but also can be used for authenticity identification of terminal products such as food, medicines, health care products and the like containing pseudo-ginseng components. Through innovative application of the Proofman-LMTIA technology, an effective solution is provided for rapid and accurate identification of pseudo-ginseng and derivative products thereof, and the method has important market application prospects and social benefits.

The invention discloses a wearable C peptide detection device as well as a detection method and application thereof. The device comprises a detection chip, and the detection chip comprises a microneedle array, a reaction cavity and an injection part which are sequentially connected through a liquid outlet channel. According to the wearable C peptide detection device, rapid detection of C peptide can be achieved within 15 min, and meanwhile, the wearable C peptide detection device can be repeatedly used after elution, so that repeated, rapid and dense data collection can be achieved, and real-time monitoring of the C peptide is achieved.

The invention provides a redried tobacco leaf mildewing detection method based on mixed attention mechanism enhancement and a 3D residual CNN network, and the method comprises the steps: collecting redried tobacco leaf samples, and preparing mildewed tobacco leaf samples in different periods; hyper-spectral image data of the mildewed and redried tobacco leaf samples are collected through hyper-spectral image collecting equipment; the collected hyperspectral image data is corrected; establishing a mildewed tobacco leaf data set according to the corrected hyperspectral image data; an MA3D-ResNet model is constructed; carrying out iterative training on the model by utilizing the training set and the verification set, and storing model parameters with the highest accuracy on the verification set within a specified number of iterations; model parameters which are obtained through training and have the highest accuracy rate are imported into the model, test set data are input, evaluation indexes are calculated according to a model output result, the corrected hyperspectral image of the redried tobacco leaves to be detected is input into the tested model, and a mildew detection result is obtained. The method can effectively improve the detection precision of the mildewing degree of tobacco leaves with extremely similar spectral characteristics, especially early mildewing tobacco leaves.

The invention discloses a method for detecting and quantifying impurity residues in a polyethylene glycol-polycaprolactone triblock copolymer. Impurities are a polyethylene glycol-polycaprolactone diblock copolymer and residual polyethylene glycol. The method comprises the following steps: derivatizing, separating and purifying a polymer and impurities, performing nuclear magnetic detection to detect impurities which are difficult to detect by a conventional analysis method, and calculating the content of the impurities according to a peak area ratio of a polymer derivatization functional group characteristic peak to an impurity derivatization functional group characteristic peak. The method is high in derivatization efficiency and simple in derivative separation, impurity residue information of the product can be effectively obtained through nuclear magnetic test calculation, and the method is a novel detection method which is simple to operate and high in practicability.

The present invention discloses a method for predicting rosin properties based on infrared spectroscopy, the method comprising: S1, collecting rosin samples from wetland pine wood, dividing the rosin samples into a first rosin sample and a second rosin sample; S2, analyzing the sample components based on the first rosin sample to obtain the rosin components; S3, collecting near-infrared spectral data of the sample based on the second rosin sample to obtain near-infrared spectral data of the rosin; S4, correlating and fitting the rosin components with the near-infrared spectral data to establish a near-infrared correction model for rosin properties; S5, using an external validation set of rosin samples that do not participate in modeling to verify the near-infrared correction model for rosin properties; S6, predicting rosin properties based on the near-infrared correction model for rosin properties to obtain a rosin property prediction result. The present invention improves the efficiency of genetic improvement of resin-producing tree species and promotes the rapid development of forest genetic improvement research.

The invention discloses a hapten for detecting the content of isofenphos-methyl and application of the hapten, particularly relates to the hapten for detecting the content of isofenphos-methyl and a preparation method of the hapten, and further discloses application of the hapten in the aspects of antibodies and kits. The enzyme linked immunosorbent assay kit established by aiming at the hapten for detecting the content of isofenphos-methyl can realize rapid detection of a large batch of samples to be detected, is convenient to use and low in detection cost, the detection method is efficient, accurate and rapid, can simultaneously detect a large batch of samples, and is suitable for on-site monitoring of isofenphos-methyl in fruits, vegetables and traditional Chinese medicinal materials and screening of a large number of samples.

The invention relates to the technical field of biology, in particular to a primer probe combination and a kit for real-time fluorescence MIRA detection of high-toxicity klebsiella pneumoniae and application of the primer probe combination and the kit. The invention provides a primer probe combination for detecting high-virulence klebsiella pneumoniae and matched products (a reagent and an MIRA detection kit). Aiming at the typical virulence gene iucA of the high-virulence klebsiella pneumoniae, the primer group and the probe suitable for real-time fluorescence MIRA detection are specifically designed, and the primer probe combination and the product are particularly suitable for on-site screening of the high-virulence klebsiella pneumoniae, are high in detection speed and good in specificity and are not interfered by false positive, cross contamination and the like. Meanwhile, the detection result is reliable, the sensitivity is high, the specificity is strong, and the kit is suitable for food inspection, disease control centers and quality supervision departments.

The present application provides a SNP molecular marker related to wet damage tolerance of Brassica napus and application thereof, wherein the sequence of the SNP molecular marker is shown as SEQ ID No. 1, and an A / G base mutation exists at 11872958 of the sequence shown as SEQ ID No. 1. The SNP molecular marker provided by the present application is based on a competitive allele-specific PCR marker detection method, and is used for detection and identification of the wet damage tolerance trait of Brassica napus. The detection method is simple in operation, low in cost, accurate in detection result, good in repeatability and stability, and different detection laboratories and different data results can be compared and verified with each other, and the data has universal comparability.

The invention belongs to the technical field of biology, and discloses a detection method of non-white proteinuria and application of the non-white proteinuria in auxiliary diagnosis of metabolic syndrome. The non-white proteinuria detection method comprises the following steps: S1, carrying out qualitative analysis on a urine sample to obtain qualitative detection results including urine pH, PRO and BLD; if the BLD is positive, judging that the urine sample is suspected non-white proteinuria; if the BLD is negative, executing the step S2; s2, according to the urine qualitative detection result, a urine sample quantitative detection mode is selected to carry out TPU and MALB quantitative detection on the urine sample, and a quantitative detection result is obtained; and jointly judging whether the urine sample is non-white proteinuria or not according to TPU and MALB quantitative detection results and PRO qualitative detection results. The invention also provides a non-white proteinuria detection system which comprises a laboratory information system, an IS system and a urine quantitative analysis system. The non-white proteinuria detection method can realize identification of white proteinuria and non-white proteinuria in proteinuria, and provides a new way for clinical diagnosis of MetS.

The application of the biomarker combination in the preparation of a drug for fulminant myocarditis belongs to the field of drugs. The application is characterized in that the biomarker combination comprises Siglec-5; the biomarker combination is further selected from the group consisting of sST2, PAI-1, Siglec-5, CD163, CD40, P-Cadherin, CD14, CTLA4. The application finds that the expression of the biomarker in the plasma of a patient with fulminant myocarditis is increased, and confirms the role of the biomarker as a drug target in relieving and / or improving fulminant myocarditis. The level of the biomarker can be controlled by a drug, the diagnosis and subsequent treatment of the disease can be more intensified, and the biomarker has great application value in clinical practice.

The invention relates to the technical field of biology, in particular to an HPLC (High Performance Liquid Chromatography) analysis method of poly-HRP and a marker thereof. The invention provides the HPLC detection method of the poly-HRP and / or the marker-labeled poly-HRP, the HPLC detection method can realize the polymerization degree of the marker-labeled poly-HRP and the detection of the marker or the HRP, and the HPLC detection method is simple, rapid, good in repeatability and convenient to control the difference between batches, so that the defects in the prior art are overcome, and the HPLC detection method has a wide application prospect.

The invention relates to a protease residue detection method which comprises the following steps: step 1, sample preparation: collecting a corresponding sample according to the type of protease to be detected to obtain a sample to be tested; step 2, reagent preparation; 3, setting a detection method, and sampling by using a wiping sampling method; step 4, signal reading: reading a signal generated by reaction through detection equipment, recording the signal reading of each sample, and providing original data for subsequent data analysis; and 5, data analysis. The detection method disclosed by the invention is rapid and convenient, the analysis and inspection time is shortened, and the detection method also has important significance in the aspect of maintenance of the cleaning verification state.

The invention provides a method for detecting the content of amantadine in a water body. The method is a liquid chromatography-tandem mass spectrometry method for detecting the content of amantadine in a water body (including seawater and fresh water), and aims at providing a rapid, sensitive and accurate detection means to meet the requirement for safety monitoring of a water body environment.

The present invention provides a quality control method for a Ruren medicinal material and a Ruren formula granule, the quality control method comprising the following steps: extracting the Ruren medicinal material or the Ruren formula granule to prepare a test solution; mixing protocatechuic acid and an ethanol aqueous solution to prepare a reference solution; respectively detecting the test solution and the reference solution by high performance liquid chromatography to obtain the content of protocatechuic acid in the Ruren medicinal material or the Ruren formula granule and the characteristic spectrum of the Ruren medicinal material or the Ruren formula granule. The present invention constructs an HPLC characteristic spectrum of the Ruren medicinal material and the Ruren formula granule, fully demonstrating the chemical composition characteristics of the Ruren medicinal material and the Ruren formula granule; at the same time, the present invention establishes an HPLC content determination method for protocatechuic acid in the Ruren medicinal material and the Ruren formula granule, which is simple to operate, high in accuracy and good in stability.

The invention belongs to the field of analysis and detection, and particularly relates to an HPLC-SEC detection method for detecting the content of deoxyribonucleic acid in androstenedione prepared by a fermentation method, the method is rapid, simple and convenient, the androstenedione and the deoxyribonucleic acid can be effectively separated, meanwhile, the low-concentration deoxyribonucleic acid can be effectively detected and accurately quantified, and the detection accuracy is high. And more reliable guarantee is provided for product quality and medication safety.

The invention belongs to the technical field of biopharmacy analysis, and provides a # imgabs0 # 13-S9 content detection method, which comprises the operation of detecting a sample to be detected by adopting an RP-UPLC-ELSD (Reverse P-Ultra Performance Liquid Chromatography-Evaporative Light Scattering Detector) method, mobile phases adopted in the RP-UPLC-ELSD method comprise a mobile phase A and a mobile phase B; wherein the mobile phase A is water, and the mobile phase B is methanol; according to the method, the mobile phase is adopted for gradient elution. According to the detection method for the content of the # imgabs1 # 13-S9, provided by the invention, the residual quantity of the # imgabs2 # 13-S9 in the recombinant protein vaccine stock solution can be accurately determined, and the detection method is rapid, accurate, linear and good in specificity, is not interfered by other components in the recombinant protein vaccine stock solution, and has guiding significance for detecting the residual quantity of the # imgabs3 # 13-S9 in a histone vaccine stock solution.

The invention belongs to the technical field of detection, and particularly discloses a method for screening anti-inflammatory and uric acid-reducing related drugs for treating gout illegally added in food and health food, a liquid chromatography-mass spectrometry detection method covers 15 acute and chronic gout drugs commonly used in domestic and overseas markets, and is high in sensitivity, convenient to operate, short in method time consumption and high in accuracy. And gout treatment related drugs illegally added in food and health food can be conveniently and rapidly identified.

The measuring circuit comprises a digital-to-analog conversion chip, a voltage sampling circuit, a current sampling circuit and a 3.3 V LDO voltage stabilizing circuit, the digital-to-analog conversion chip comprises an A0 pin, an A1 pin, an A2 pin, an A3 pin, an SCL pin and an SDA pin, the A0 pin and the A1 pin are electrically connected with a resistor R4, a resistor R5, a resistor R1, a resistor C2 and a resistor C3, the A2 pin is electrically connected with an R6, an R3, an R2 and a C4, the current sampling circuit is electrically connected with the 3.3 V LDO voltage stabilizing circuit, and the 3.3 V LDO voltage stabilizing circuit is electrically connected with the 3.3 V LDO voltage stabilizing circuit. The A3 pin is electrically connected with the R7, and the SCL pin and the SDA pin are electrically connected with the R8 and the R9. According to the utility model, a rapid, accurate and reliable detection method is provided, the method is durable and low in cost, the measuring range of sampling voltage and current can be adjusted according to actual requirements, the circuit design is simplified, and the manufacturing cost is remarkably reduced.

The invention discloses a molecular marker in a bovine KCNIP1 gene and application of the molecular marker. The molecular marker is an SNP (Single Nucleotide Polymorphism) site g.2487025Ggt in a bovine genome; a. The molecular marker or the substance for detecting the single nucleotide polymorphism of the cattle molecular marker provided by the invention can be applied to cattle genetic breeding, so that the number of red blood cells and the hemoglobin concentration of improved cattle offspring in Tibetan are reduced, and the problem of hypoxia faced by cattle breeding in a plateau environment is solved.

The invention provides a porous laser-induced graphene electrode, a preparation method, a product and application, and belongs to the technical field of environmental protection. The preparation method of the graphene electrode based on porous laser induction comprises the following steps: carrying out laser direct writing on the surface of an organic polymer film, and collecting a black material when the surface of the organic polymer film becomes black; and dispersing the black material in a solution, then coating the surface of a substrate electrode with the obtained dispersion liquid, and drying to obtain the porous laser-induced graphene electrode. According to the present invention, the porous laser-induced graphene electrode is adopted as the working electrode, and the porous laser-induced graphene electrode is adopted as the electrochemical sensor so as to be applied to detection of BPA in the actual environmental sample, such that the wide detection range, the low detection limit, the high sensitivity, and the good repeatability and the good stability are provided;

The application belongs to the technical field of disease diagnosis reagent, and relates to application of a reagent for detecting a marker THBS1 in preparation of a type 2 cardiorenal syndrome diagnosis reagent. In view of the problems of difficulty in diagnosis of type 2 cardiorenal syndrome and insufficient risk assessment in the prior art, a new biomarker THBS1 is found, and the correlation of the marker with type 2 cardiorenal syndrome is confirmed. Therefore, the reagent for detecting the marker THBS1 can be used for preparation of a related reagent for diagnosis and / or evaluation of prognosis of type 2 cardiorenal syndrome. It is found through an animal model that the level of THBS1 in plasma of a type 2 cardiorenal syndrome model mouse is significantly increased, and it is confirmed that THBS1 can be used as a biomarker for diagnosis of type 2 cardiorenal syndrome. Therefore, the level of THBS1 in plasma can be detected to assist in diagnosis of type 2 cardiorenal syndrome or evaluation of prognosis of type 2 cardiorenal syndrome.

The present application provides a molecular marker in the ADAM18 gene of cattle and its application. The molecular marker is one or more of g.34873341T>A, g.34874222C>T and g.34877973C>G in the cattle genome. The molecular marker or the material used to detect the single nucleotide polymorphism of the molecular marker of cattle provided by the present application can be used in cattle genetic breeding to reduce the hemoglobin concentration of the offspring of Tibetan improved cattle and solve the low oxygen bottleneck problem faced by the breeding of plateau cattle.

The invention discloses a COVID-19 nucleic acid detection primer group and a COVID-19 isothermal amplification nucleic acid detection kit, and relates to the technical field of virus nucleic acid detection methods. The invention provides a detection kit of COVID-19, the detection kit is constructed by using the nucleic acid detection primer group of the COVID-19, nucleic acid extraction is not needed, the COVID-19 virus can be accurately and rapidly detected within one hour under an isothermal condition through a specific chromogenic reaction, the infection condition of the COVID-19 virus can be conveniently and intuitively screened, and the detection kit has the advantages that the detection kit is simple in structure and convenient to operate, and the detection kit has a good application prospect. The method has wide prospects and great social benefits.

The present invention discloses a detection device and a detection method for quantitative analysis of cigarette fly ash. The detection device comprises: a simulated human body suction unit (1), a fly ash collection unit (2), an ash flicking unit (3), an image acquisition unit (4), an image processing unit and a workbench (6). The present invention comprehensively considers the fly ash area of ​​the cigarette during the suction movement and the ash flicking process as an indicator of the cigarette fly ash amount. In the present invention, the image acquisition unit (4) collects in real time the cigarette fly ash images on the fly ash collection plate (21) before and after the ash flicking, and the image processing unit analyzes the collected images and calculates the cigarette fly ash amount during the cigarette movement and suction process. In the cigarette fly ash detection device of the present invention, the image acquisition unit (4) directly collects the cigarette fly ash images falling on the fly ash collection plate (21), so as to more intuitively examine the fly ash amount of the cigarette during the suction movement and the ash flicking process. The detection method is fast, accurate, easy to promote, and the result is more accurate.

The invention belongs to the technical field of detection, and provides a Triton N-101 content determination method, which comprises the operation of detecting a to-be-detected sample by adopting an RP-HPLC-ELSD (Reverse P-High Performance Liquid Chromatography-Evaporative Light Scattering Detector) method, mobile phases adopted in the RP-HPLC-ELSD method comprise a mobile phase A, a mobile phase B and a mobile phase C; wherein the mobile phase A is water, the mobile phase B is methanol, and the mobile phase C is isopropanol; according to the method, the mobile phase is adopted for gradient elution. The method provided by the invention can be used for accurately determining the residual quantity of Triton N-101 in the subunit influenza vaccine containing the milk adjuvant, is rapid, accurate and good in linearity and specificity, and is not interfered by all components of the milk adjuvant and other components in components of a sample preparation.

The invention discloses a method and a kit for detecting sodium cyclamate in baijiu, and belongs to the technical field of food safety detection. The hapten is obtained, the sodium cyclamate monoclonal antibody is prepared according to the hapten, the enzyme linked immunosorbent assay kit is further prepared through specific binding of the sodium cyclamate monoclonal antibody and sodium cyclamate, and qualitative and quantitative detection can be conducted on sodium cyclamate in baijiu. The detection method is rapid, efficient and high in sensitivity, the lowest detection limit of sodium cyclamate in various types of baijiu is as low as 2.75-2.82 mu g / L, the detection variable coefficient RSD is smaller than 5%, the detection result is accurate and sensitive, and the problems that an existing detection method is complex in sample pretreatment step, high in instrument and equipment condition requirement and the like are effectively solved.

The present invention discloses a method for rapidly detecting protein-bound BMAA in marine diatoms, comprising the following steps: firstly, full-wavelength scanning of the expressed and purified cysK enzyme is performed to determine the absorbance of the cysK protease; then, a method for detecting the cysK enzyme in diatom cells based on absorbance is established, and the concentration of the cysK enzyme in the diatom cells is detected at a specific wavelength (450nm); and the content of BMAA in the corresponding diatoms is determined by the LC-MS / MS method; finally, the linear relationship between the cysK enzyme concentration in the diatom cells and the amount of BMAA synthesis in the diatom cells is evaluated, so as to achieve the purpose of rapidly estimating the protein-bound BMAA in the diatom cells based on the absorbance method. There is no method for rapidly quantifying algal toxins using important synthases in algae, and the present invention can solve the problems of low efficiency and high cost of detecting BMAA toxins in diatoms to a certain extent.

The application relates to the field of crystal structure symmetry classification and retrieval, and specifically discloses a crystal structure symmetry determination and retrieval method using electron diffraction, which comprises the following steps: a multi-view selected area electron diffraction data input module is used to acquire a two-zone axial diffraction spectrum and perform pretreatment; a symmetry feature coding module is used to extract a single-view descriptor through a double-branch convolutional neural network, to obtain a material descriptor through view pooling fusion; a hierarchical symmetry classification module is used to realize hierarchical classification of crystal systems and space groups, and to guarantee the logic of the results through consistency constraints; a high-dimensional feature embedding and database construction module is used to standardize crystal structure files, to generate descriptors, and to construct a vector database; and a constrained structure retrieval module is used to combine the classification results and user constraints to filter candidate structures, and to complete retrieval through cosine similarity. The application solves the problems of poor generalization, insufficient multi-view feature fusion and inability to associate a crystal library in the prior art, and realizes full-process automation of crystal structure symmetry classification and retrieval.

The present invention belongs to the technical field of molecular marker-assisted selection of goats, and particularly relates to a SNP molecular marker associated with the number of lambs in the FSHB gene of goats and its application. The goat SNP site is g.21919354C>T, and the nucleotide sequence of the molecular marker is shown in SEQ ID NO.1. The allele type of the SNP molecular marker is T, which can significantly improve the number of lambs of goat offspring, and provide a new molecular marker for the detection of the number of lambs of goats or molecular marker-assisted breeding.