Characteristic wave bands based method and device for rapidly detecting content of proteins in black fungi

A technology of protein content and characteristic waveband is applied in the detection field of black fungus protein content, which can solve the problems of time-consuming measurement process, high cost of consumables, chemical pollution, etc., and achieve the effect of reducing detection cost, low cost and simple detection process.

Inactive Publication Date: 2010-05-12
ZHEJIANG UNIV
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Problems solved by technology

The traditional protein measurement method is Kjeldahl method or combustion nitrogen method, the measurement process is time-consuming, laborious, high cost of consumables, and the intermediate process is prone to chemical pollution

Method used

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  • Characteristic wave bands based method and device for rapidly detecting content of proteins in black fungi
  • Characteristic wave bands based method and device for rapidly detecting content of proteins in black fungi
  • Characteristic wave bands based method and device for rapidly detecting content of proteins in black fungi

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Embodiment Construction

[0024] Such as figure 1 As shown, a black fungus protein content rapid detection device based on characteristic bands includes a spectrum acquisition device 6, an embedded system 7 and a power supply system 8, the light source acquisition device 6 is connected to the embedded system 7 through a USB data line, and the embedded system 7 is powered by a power supply system 8 .

[0025] The spectrum acquisition device 6 consists of a sample cell 1, a quartz glass slide 2 covered on the sample cell 1, a photosensitive sensor fixed layer 3 above the quartz glass slide 2, a photosensitive sensor combination device 4 fixed by the photosensitive sensor fixed layer 3, and a light source system 5 Composition; the photosensitive sensor fixed layer 3 is provided with 8 channels uniformly distributed along the same circumference, the center line of each channel is 45° to the vertical direction, and meets the irradiation center of the light source system 5 on the quartz glass slide 2; the ph...

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Abstract

The invention discloses a characteristic wave bands based method for rapidly detecting the content of proteins in black fungi. The method comprises the following steps: 1) grinding and then sieving the black fungi; 2) acquiring the reflectance values of the black fungi respectively corresponding to the wavelength 1318nm, 1100nm, 1396nm, 2214nm, 1834nm, 1520nm and 2498nm; 3) converting the obtained reflectance values into absorbency values; 4) putting the absorbency values into the following linear equation respectively: Y=89.960X1-248.482X2+269.366X3-68.022X4+200.063X5-309.675X6+87.126X7+2.028, and obtaining the content of the proteins in the black fungi through computation, wherein in the equation, Y is the content of the proteins in the black fungi, and X1-X7 respectively represent the absorbency values of the black fungi respectively corresponding to the wavelength 1318nm, 1100nm, 1396nm, 2214nm, 1834nm, 1520nm and 2498nm. The invention also discloses a device for implementing the above method. The invention is mainly used for rapidly and accurately detecting the content of the proteins in the black fungi through the characteristic wave bands, is rapid and accurate in detection, greatly shortens the detection time, reduces the environmental pollution and lowers the detection cost.

Description

technical field [0001] The invention relates to a method and device for detecting protein content of black fungus, in particular to a method and device for rapidly detecting protein content of black fungus based on a characteristic band model. Background technique [0002] Black fungus is rich in nutrition, contains more carbohydrates, protein, fat, cellulose and other nutritional components, and is widely eaten as a food with health care functions. The quality of black fungus varies greatly due to the influence of the growth environment (altitude, temperature and humidity, climate conditions, etc.) of its place of origin. Protein is an important indicator of the quality of black fungus, and its rapid detection is very important for the quality control of black fungus. The traditional protein measurement methods are Kjeldahl method or combustion nitrogen method. The measurement process is time-consuming, labor-intensive, and the cost of consumables is high, and the intermed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/35G01N21/01G01N21/3563G01N21/359
Inventor 何勇刘飞
Owner ZHEJIANG UNIV
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