19 results about "Protein measurement" patented technology

The utility model relates to the field of culture solution concentration detection, in particular to an on-line protein concentration detection device for expanding a concentration range. The device comprises a first injection pump, a selector valve, a container assembly, a dilution and detection assembly and an air filter, the selector valve is provided with a public port and six selection ports uniformly distributed around the public port, and the public port is communicated with one of the six selection ports; a buffer ring is communicated between the first injection pump and the common port of the selection valve; the container assembly comprises a calibration standard liquid container, a first cleaning liquid container, a second cleaning liquid container and a culture container; the dilution and detection assembly comprises a three-way valve, a dilution assembly and a detection assembly respectively communicated with two ends of the three-way valve, and a sample reserving valve communicated with the output end of the detection assembly. According to the utility model, the detection concentration range is expanded to 6.5 orders of magnitude, the requirement of the protein measurement concentration range from extremely low concentration to extremely high concentration in the whole cell culture process is met, and the measurement accuracy and sensitivity are high.

The invention provides a rapid determination method for precipitated protein in yellow rice wine. Comprising the following steps: (1) pretreating yellow wine; (2) pretreatment of an ultrafiltration tube; (3) intercepting precipitated protein; and (4) determining the content of precipitated protein. The invention provides a rapid determination method of precipitated protein in yellow rice wine, which can physically separate and remove free amino acid, oligopeptide and other small molecular substances interfering with trace protein determination based on ultrafiltration tube centrifugal treatment, thereby rapidly determining the content of protein really participating in yellow rice wine precipitation formation. The specificity and the accuracy of a detection result are effectively improved.

The invention belongs to the technical field of biotechnology and protein determination, and particularly relates to confining liquid as well as a preparation method and application thereof. Modified bovine serum albumin and recombinant casein polypeptide are used as core components of the confining liquid, hydroxypropyl-beta-cyclodextrin, polyglycerol fatty acid ester and other auxiliary components are added, and the confining liquid is prepared through a specific technology. The modified bovine serum albumin and the recombinant casein polypeptide have a synergistic effect, so that active sites of the detection carrier can be efficiently sealed; the stabilizing agent and other components are supplemented, so that the stability and compatibility of the confining liquid are improved. The confining liquid is simple and convenient in preparation process, controllable in cost, suitable for various detection carriers such as elisa plates and nitrocellulose membranes, capable of reducing non-specific binding and improving detection sensitivity, and suitable for various biological detection scenes such as enzyme-linked immunosorbent assay.

The invention relates to a detection method of B-raf nucleic acid V600E point mutation and a corresponding product. The method for detecting the V600E point mutation of B-raf nucleic acid based on RPA / RAA / ERA / MIRA-CRISPR / Cas12a comprises the following steps: in the same container, contacting a sample to be detected with the composition, the product combination, the reagent, the kit, the system or a part for RPA / RAA / ERA / MIRA nucleic acid amplification and a part for eliminating B-raf wild type nucleic acid in the system, and then contacting with the part for CRISPR nucleic acid detection; more preferably, the first V-A type Cas protein is inactivated before the first V-A type Cas protein is contacted with the part for CRISPR nucleic acid detection. A change in the signal is determined. Compared with the prior art, a one-tube two-step CRISPR nucleic acid detection system for the V600E point mutation of the B-raf nucleic acid is established, the detection time is 45 minutes, and the CRISPR nucleic acid detection system has the characteristics of rapidness in detection, convenience in operation and the like, and also has relatively high detection sensitivity.

The invention discloses a method for improving the stability of peanut butter, and belongs to the technical field of food processing. The invention provides a solution aiming at the problems that the peanut butter is easy to separate out grease and harden in texture in the storage process. The method comprises the following steps: by taking peanut protein powder as a raw material, preparing peanut protein isolate by an alkali-solution and acid-isolation method; measuring the contents of protein, fat and water in the peanut kernels, selecting baking temperature and time according to the measurement result, baking, removing red skin, and grinding to obtain a peanut butter base material; measuring the wet basis protein content and the moisture content of the peanut butter base material, selecting the addition amount of the peanut protein isolate in a range of 2-3% of the mass of the base material, and adding the peanut protein isolate into the base material to enable the wet basis total protein content of the mixture to reach 30-33%; and finally, stirring and mixing the mixture, and standing to obtain the stabilized peanut butter. The method is mainly used for improving the grease separation problem of the peanut butter and improving the storage stability of the peanut butter.

The invention relates to a glial fibrillary acidic protein (GFAP) determination kit, and relates to the technical field of biomedical detection, the glial fibrillary acidic protein determination kit comprises an anti-glial fibrillary acidic protein single domain antibody, the anti-glial fibrillary acidic protein single domain antibody comprises VHH, the VHH comprises CDR1, CDR2 and CDR3 in an amino acid sequence shown as SEQ ID NO: 1; the kit also comprises magnetic particles; wherein the magnetic particles are coupled with the anti-colloidal fibrillary acidic protein single-domain antibody. According to the present invention, the single-domain antibody capable of specifically recognizing the glial fibrillary acidic protein is adopted as the capture antibody, and the magnetic particle separation and chemiluminescence detection technology is combined, such that the high-sensitivity, high-specificity, rapid and stable quantitative detection of the glial fibrillary acidic protein in the cerebrospinal fluid, the serum or the plasma is achieved, and the important clinical application prospect is provided.

The specific protein analyzer comprises a sample supply device, a first reagent supply device, a second reagent supply device, a reaction tank, a uniform mixing assembly, a cache assembly, a detection device and a control device. The sample supply device, the first reagent supply device and the second reagent supply device are respectively used for supplying a blood sample to be detected, a first reagent and a second reagent into the reaction tank. The uniform mixing assembly is used for uniformly mixing the mixed sample liquid, then the control device is used for controlling the caching assembly to suck out the uniformly mixed sample liquid in the middle of the reaction tank for caching, and specific protein content detection of the detection device is received. The uniformly mixed sample liquid in the middle of the reaction tank is relatively uniform and contains a small number of bubbles, so that a more accurate detection effect can be obtained. The invention further provides a specific protein determination method and a computer readable storage medium for implementing the method.

The present application provides a specific protein analyzer, which includes a sample supply device, a first reagent supply device, a second reagent supply device, a reaction cell, a mixing assembly, a buffer assembly, a detection device, and a control device. The sample supply device, the first reagent supply device, and the second reagent supply device are respectively used to supply a blood sample to be tested, a first reagent, and a second reagent to the reaction cell. The mixing assembly mixes the mixed sample solution, and then the control device controls the buffer assembly to suck out the mixed sample solution in the middle of the reaction cell for buffering, and accepts the specific protein content detection by the detection device. The mixed sample solution in the middle of the reaction cell is relatively uniform and contains fewer bubbles, so more accurate detection results can be obtained. The present application also provides a specific protein determination method and a computer-readable storage medium for implementing this method.

The invention discloses full-automatic soybean component infrared detection equipment based on a mechanical arm and a use method. The full-automatic soybean component infrared detection equipment comprises a rotary worktable, wherein a plurality of groups of soybean sample containers are loaded on the rotary worktable; a six-axis mechanical arm is arranged on one side of the rotary workbench, and the six-axis mechanical arm can grab a soybean sample container on the rotary workbench and put a soybean sample in the soybean sample container into an infrared tester for detection; the initial detection camera assembly is used for detecting the sealing film integrity of the soybean sample container; the soybean sample container is shaken through the material uniformizing mechanism, so that the soybean sample particles in the soybean sample container are clear; the sealing film at the top of the soybean sample container is separated through the film cutting mechanism; and the final inspection camera assembly is used for detecting empty bottle residues of the soybean sample container. And by matching with a near-infrared soybean protein tester, unmanned full-automatic detection of soybean components is really realized in a clean laboratory.

Provided herein are compositions, methods, and kits suitable for detection and / or quantification of calprotectin in a sample (including extracts thereof). In some aspects, compositions, methods, and / or kits comprise a Fab-Enabled Split Luciferase Calprotectin Assay (FESCA) or components thereof, a novel quantitative method for calprotectin measurement. In some aspects, provided herein are two new fragment antigen binding proteins (Fabs), CP16 and CP17, which bind to different epitopes of the calprotectin complex. These Fabs can be fused, directly or indirectly, with a reporter system, such as split NanoLuciferase fragments, enabling the reconstitution of active luciferase upon binding to calprotectin either in solution or in varied immobilized assay formats. Technologies provided herein can be utilized to detect physiologically relevant calprotectin levels across various sample types, including serum, plasma, whole blood, and stool extracts, and can be utilized in methods of treating a subject.

The application relates to a cell strain stability prediction method and device, computer equipment and a storage medium. The method comprises the following steps: obtaining an observation image of a cell strain to be predicted; inputting the observation image into a stability prediction model for processing to obtain a stability value of the cell strain to be predicted or a stability category determined according to the stability value; the stability prediction model is determined according to images of a plurality of historical cell strains and a stability value or a stability category determined according to the stability value of each historical cell strain; and the stability value or the stability category determined according to the stability value is used to represent the stability of the protein expression amount of the cell strain. When the prediction result of the stability value of the cell strain or the stability category determined according to the stability value is determined by using the prediction method of the application, the manual multi-generation culture and a large amount of protein measurement experiment are not needed, the stability prediction model can be relied on to realize the prediction of the stability of the cell strain, and the stability prediction efficiency of the cell strain is greatly improved.

Methods for the detection and quantification of target molecules, such as proteins, in biological samples are provided. The disclosed method includes capturing a target molecule by an aptamer, replacing the aptamer with an aptamer identification sequence, and then sequencing the aptamer identification sequence by a new generation sequencing technique.

This invention discloses a cerebrospinal fluid or urine total protein detection kit, belonging to the technical field of detection kits. The kit includes reagent R1 and reagent R2, wherein reagent R1 includes a buffer stabilizer Tris to maintain the stability of reagent R1 during the opening process. This invention solves the problems of easy pH drop and poor stability of existing turbidimetric total protein assay kits after opening. The cerebrospinal fluid or urine total protein detection kit, by adding Tris to reagent R1, can maintain the stability of small amounts of reagent after opening, which is beneficial for further promotion and use in the market, ensuring the long-term reliability of test results and the economy of reagent use.

The invention belongs to the technical field of biotechnology and protein determination, and particularly relates to a rapid membrane transfer method. The method comprises the following steps: firstly, soaking gel in a pretreatment solution, sequentially soaking a solid-phase membrane in methanol and an equilibrium solution, then assembling a membrane transfer device, adding a membrane transfer buffer solution, and transferring the membrane by adopting a gradient electric field. Through the synergistic effect of gel pretreatment, optimization of a membrane transfer buffer solution formula and a gradient electric field membrane transfer mode, the membrane transfer time is shortened, and the experiment efficiency is greatly improved. According to the membrane transfer method, the transfer efficiency of proteins with the molecular weight of 10-200 kDa reaches 90% or above, the problems that high-molecular-weight proteins are incompletely transferred and small-molecular-weight proteins are prone to penetrating in an existing method are solved, the sensitivity and accuracy of subsequent detection are remarkably improved, and the membrane transfer method is suitable for large-scale application and popularization.

The field of extracellular vesicles (EVs) currently attracts substantial attention in biomedicine due to the proposed role of EVs in various biological processes and their potential of serving as biomarkers for diseases. However, the availability of approaches for reliable and reproducible standardised quantification of EVs is limited. Therefore, current interest in EV research urges reliable tools of standardization and accurate enumeration of EVs, preferably on the basis of lipid quantification. By definition, EVs are surrounded by phospholipid bilayers, therefore, lipids (such as phospholipids and cholesterol) are essential components of all EVs. The method of the invention avoids the overestimation of EV concentration based on the protein measurement, as it focuses on the defining component of EVs, the lipid bilayer. Our method can be used virtually in any standard laboratories where a fume hood, a thermoblock, and a spectrophotometer are available. The application does not require expensive equipment, therefore it can be an easy, reliable and quick method for quantification of EVs and standardisation of EV experiments.

Methods for detection and quantification of target molecules, such as proteins, in a biological sample are provided. The disclosed methods include capturing a target molecule by an aptamer, replacing the aptamer with an aptamer identifier sequence, and then sequencing the aptamer identifier sequence by a next generation sequencing technology.

According to an embodiment of the present disclosure, a medium with which blood plasma is separated and stored, the medium including a porous medium for separation of blood plasma and a substance supported on the porous medium, the substance that suppresses glycation of a protein by glucose, is provided.

Tri-molecular complexes and related systems for quantifying the abundances of target molecules in a biological sample are provided. The disclosed tri-molecular complexes each include an aptamer, a first capture probe hybridized to a first portion of the aptamer, and a second capture probe hybridized to a second portion of the aptamer. The second capture probe further includes an aptamer identification sequence that may be used to identify the aptamer, and thereby to identify the target molecule to which the aptamer is configured to bind.