6 results about "Protein measurement" patented technology

The invention belongs to the technical field of biotechnology and protein determination, and particularly relates to confining liquid as well as a preparation method and application thereof. Modified bovine serum albumin and recombinant casein polypeptide are used as core components of the confining liquid, hydroxypropyl-beta-cyclodextrin, polyglycerol fatty acid ester and other auxiliary components are added, and the confining liquid is prepared through a specific technology. The modified bovine serum albumin and the recombinant casein polypeptide have a synergistic effect, so that active sites of the detection carrier can be efficiently sealed; the stabilizing agent and other components are supplemented, so that the stability and compatibility of the confining liquid are improved. The confining liquid is simple and convenient in preparation process, controllable in cost, suitable for various detection carriers such as elisa plates and nitrocellulose membranes, capable of reducing non-specific binding and improving detection sensitivity, and suitable for various biological detection scenes such as enzyme-linked immunosorbent assay.

The invention relates to a detection method of B-raf nucleic acid V600E point mutation and a corresponding product. The method for detecting the V600E point mutation of B-raf nucleic acid based on RPA / RAA / ERA / MIRA-CRISPR / Cas12a comprises the following steps: in the same container, contacting a sample to be detected with the composition, the product combination, the reagent, the kit, the system or a part for RPA / RAA / ERA / MIRA nucleic acid amplification and a part for eliminating B-raf wild type nucleic acid in the system, and then contacting with the part for CRISPR nucleic acid detection; more preferably, the first V-A type Cas protein is inactivated before the first V-A type Cas protein is contacted with the part for CRISPR nucleic acid detection. A change in the signal is determined. Compared with the prior art, a one-tube two-step CRISPR nucleic acid detection system for the V600E point mutation of the B-raf nucleic acid is established, the detection time is 45 minutes, and the CRISPR nucleic acid detection system has the characteristics of rapidness in detection, convenience in operation and the like, and also has relatively high detection sensitivity.

The invention discloses a method for improving the stability of peanut butter, and belongs to the technical field of food processing. The invention provides a solution aiming at the problems that the peanut butter is easy to separate out grease and harden in texture in the storage process. The method comprises the following steps: by taking peanut protein powder as a raw material, preparing peanut protein isolate by an alkali-solution and acid-isolation method; measuring the contents of protein, fat and water in the peanut kernels, selecting baking temperature and time according to the measurement result, baking, removing red skin, and grinding to obtain a peanut butter base material; measuring the wet basis protein content and the moisture content of the peanut butter base material, selecting the addition amount of the peanut protein isolate in a range of 2-3% of the mass of the base material, and adding the peanut protein isolate into the base material to enable the wet basis total protein content of the mixture to reach 30-33%; and finally, stirring and mixing the mixture, and standing to obtain the stabilized peanut butter. The method is mainly used for improving the grease separation problem of the peanut butter and improving the storage stability of the peanut butter.

Methods for the detection and quantification of target molecules, such as proteins, in biological samples are provided. The disclosed method includes capturing a target molecule by an aptamer, replacing the aptamer with an aptamer identification sequence, and then sequencing the aptamer identification sequence by a new generation sequencing technique.

This invention discloses a cerebrospinal fluid or urine total protein detection kit, belonging to the technical field of detection kits. The kit includes reagent R1 and reagent R2, wherein reagent R1 includes a buffer stabilizer Tris to maintain the stability of reagent R1 during the opening process. This invention solves the problems of easy pH drop and poor stability of existing turbidimetric total protein assay kits after opening. The cerebrospinal fluid or urine total protein detection kit, by adding Tris to reagent R1, can maintain the stability of small amounts of reagent after opening, which is beneficial for further promotion and use in the market, ensuring the long-term reliability of test results and the economy of reagent use.

The invention belongs to the technical field of biotechnology and protein determination, and particularly relates to a rapid membrane transfer method. The method comprises the following steps: firstly, soaking gel in a pretreatment solution, sequentially soaking a solid-phase membrane in methanol and an equilibrium solution, then assembling a membrane transfer device, adding a membrane transfer buffer solution, and transferring the membrane by adopting a gradient electric field. Through the synergistic effect of gel pretreatment, optimization of a membrane transfer buffer solution formula and a gradient electric field membrane transfer mode, the membrane transfer time is shortened, and the experiment efficiency is greatly improved. According to the membrane transfer method, the transfer efficiency of proteins with the molecular weight of 10-200 kDa reaches 90% or above, the problems that high-molecular-weight proteins are incompletely transferred and small-molecular-weight proteins are prone to penetrating in an existing method are solved, the sensitivity and accuracy of subsequent detection are remarkably improved, and the membrane transfer method is suitable for large-scale application and popularization.