30 results about "Protein measurement" patented technology

The invention relates to a CRP (C-Reactive Protein) assay kit, and a preparation method and application thereof, and belongs to the technical field of IVD reagents (In Vitro Diagnostic Reagents). TheCRP assay kit provided by the invention comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises buffer solutions, inorganic salts, surfactants, stabilizing agents and preservatives;and the reagent R2 comprises buffer solutions, surfactants, sealing agents, anti-human CRP antibody latex conjugates and preservatives. The CRP assay kit provided by the invention has the advantages that on the basis of the existing latex immunoturbidimetry, a reaction system is optimized by a specific preparation method through an anti-freezing agent; the detection accuracy, the analysis sensitivity and precision of tested reagents are further improved; the research and development cost of the reagents are not improved; and the CRP assay kit can easily adapt to the development of the modern clinical laboratory medicine.

The invention discloses a determination method for the absorption and transportation amount of six components in rhizoma bletillae in a Caco-2 cell model. The method comprises the steps of: preparing a rhizoma bletillae extract solution, a standard solution serving as a reference substance and an internal standard solution; establishing a human-derived colon adenocarcinoma cell line Caco-2 cell model; preparing a cell suspension through a Caco-2 cell model; determining the content of the six components by UPLC-MS/MS; determining the total protein content according to a Coomassie brilliant blue dye liquor protein determination kit method, and calculating the cell uptake X=the total protein of a to-be-determined substance. The invention adopts UPLC-MS/MS to establish the analysis method for the 6 components in a rhizoma bletillae extract, determines the influence of the rhizoma bletillae extract to absorption and uptake of Caco-2 cells under the conditions of time, concentration, temperature, pH and P-gh inhibitors, preliminarily evaluates the in vivo absorption characteristics of the rhizoma bletillae extract, and provides scientific basis for the research and development of the oral preparation of the rhizoma bletillae extract.

The present invention relates to a technique of measuring a protein based on a degree of coloring in a liquid sample mixed with a protein measurement indicator. In the present invention, information reflecting creatinine concentration in the liquid sample is obtained, and then an influence quantity caused by creatinine to the protein concentration measurement is eliminated based on the information.

The invention provides an immune repertoire information analysis method for detecting BCR heavy and light chains in blood. The method specifically comprises the steps of constructing a reference sequence, preprocessing sequencing data, merging Paired Reads, comparing data after Merge with the reference sequence, filtering a comparison result, and performing statistical analysis. Meanwhile, the invention further provides an immune repertoire method for detecting the blood BCR. The information analysis method is adopted in the method, sequencing analysis of a CDR3 region sequence is realized bydesigning a primer combination of the blood BCR heavy and light chains; the method is used for detecting the BCR immune repertoire heavy chain and light chain of rabbits for the first time. CDR3 sequence information related to antibody generation is studied through immune repertoire sequencing of blood B cells, the law of antibody generation is understood more deeply, and meanwhile, a new way forefficiently and rapidly synthesizing specific antibodies in vitro is explored by combining technologies such as gene cloning, cell transfection, protein determination and purification.

The invention provides a C-reactive protein determination reagent. The C-reactive protein determination reagent comprises a C-reactive protein detection reagent R1, a C-reactive protein detection reagent R2 and a C-reactive protein calibrator; buffer solution is prepared from 4- hydroxyethylpiperazine ethane sulfonic acid-sodium hydroxide buffer solution, trihydroxymethyl aminomethane-hydrochloricacid buffer solution, 3-(N-morpholinyl)-2-hydroxyl propanesulfonic acid-sodium hydroxide buffer solution and phosphate buffer solution according to mass ratio of 1:1:1:1; the C-reactive protein immune gel emulsion microspheres are polystyrene microspheres, preservative is one or two of proclin 300, gentamicin and chloramphenicol. The buffer solution used for the reagent provided by the inventionis prepared from the 4- hydroxyethylpiperazine ethane sulfonic acid-sodium hydroxide buffer solution, the trihydroxymethyl aminomethane-hydrochloric acid buffer solution, the 3-(N-morpholinyl)-2-hydroxyl propanesulfonic acid-sodium hydroxide buffer solution and the phosphate buffer solution according to mass ratio of 1:1:1:1, and the reagent is remarkable effects; and the reagent has the advantages of high accuracy, high measurement precision, high analysis sensitivity, and so on.

The invention discloses a specific protein analyzer. The specific protein analyzer comprises a sample supply device, a first reagent supply device, a second reagent supply device, a reaction tank, a uniform mixing assembly, a cache assembly, a detection device and a control device, wherein the sample supply device, the first reagent supply device and the second reagent supply device are respectively used for supplying a blood sample to be detected, a first reagent and a second reagent into the reaction tank; the uniform mixing assembly is used for uniformly mixing the mixed sample liquid, then the control device is used for controlling the cache assembly to suck out the mixed sample liquid in the middle of the reaction tank for caching, and specific protein content detection of the detection device is received. The mixed sample liquid in the middle of the reaction tank is relatively uniform and contains a small number of bubbles so that a more accurate detection effect can be obtained. The invention further provides a specific protein determination method and a computer readable storage medium for implementing the method.

The invention provides a database for rapidly detecting mutton quality based on near infrared and an establishment method thereof, and relates to the technical field of mutton quality detection. The database comprises a qualitative identification module, a crude protein determination module, a crude fat determination module, a fatty acid determination module, an amino acid determination module anda grading module. According to the invention, by establishing a mutton database containing crude protein, crude fat, amino acid, fatty acid and other nutritional indexes, meat color, brightness, PH and other physical indexes, and corresponding spectral information of mutton, the operation is simple, the cost is low, the purposes of quantitative analysis of nutritional ingredients and quality grading can be achieved through simple spectral scanning, and meanwhile, a reliable detection model is also provided, the detection accuracy of crude protein, crude fat, amino acid and fatty acid of nutritional indexes is extremely high, the blank of quantitative indexes of mutton grading is filled, and the grading accuracy is 95% or above.

The invention provides a method for evaluating influences of biological protein on apolygus lucorum. The method includes the steps of firstly, diluting biological protein to be measured to obtain biological protein with different concentrations, mixing the biological protein with apolygus lucorum artificial fodders to obtain biological protein measurement fodders, and taking fodders where biological protein is not added as a contrast; secondly, wrapping the measurement fodders and the contrast fodders with extended sealing films respectively to obtain capsules; thirdly, adhering the capsules to the side wall of a raising box without cover; fourthly, inoculating nymphs of apolygus lucorum into the raising box, sealing the opening of the raising box through gauze, and conducting cultivation; fifthly, recording the death condition of apolygus lucorum or the weight changes of apolygus lucorum from the day 0, and thereby evaluating the influences of biological protein on apolygus lucorum. The method is wide in application range, agents with stomach toxicity and systemic action and chemical substances dissolved in the artificial fodders can be effectively measured, the problem that piercing-sucking mouthpart insects including apolygus lucorum can hardly make direct contact with biological protein with different concentrations is solved, the influences of biological protein on apolygus lucorum can be accurately measured, and the method has high practicability.

The field of extracellular vesicles (EVs) currently attracts substantial attention in biomedicine due to the proposed role of EVs in various biological processes and their potential of serving as biomarkers for diseases. However, the availability of approaches for reliable and reproducible standardised quantification of EVs is limited. Therefore, current interest in EV research urges reliable tools of standardization and accurate enumeration of EVs, preferably on the basis of lipid quantification. By definition, EVs are surrounded by phospholipid bilayers, therefore, lipids (such as phospholipids and cholesterol) are essential components of all EVs. The method of the invention avoids the overestimation of EV concentration based on the protein measurement, as it focuses on the defining component of EVs, the lipid bilayer. Our method can be used virtually in any standard laboratories where a fume hood, a thermoblock, and a spectrophotometer are available. The application does not require expensive equipment, therefore it can be an easy, reliable and quick method for quantification of EVs and standardisation of EV experiments.

The present invention provides methods for quantitation of glycated in a biological sample using a solid support matrix by making a first bound protein measurement total bound protein under conditionswhere both glycated and non-glycated protein bind to the support and making a second bound protein measurement under conditions where glycated protein is bound to the support and non-glycated proteinis not substantially bound. Diagnostic devices and kits comprising the methods of the present invention are also provided.

Disclosed is a digestion device for protein measurement, which relates to a digestion device. A right-angle container is formed in the front end face of a chassis; an electric appliance control box is arranged at one side of the chassis and at an end part of the right-angle container; a plurality of transverse foundation pillars are arranged in the right-angle container in parallel and are connected to the chassis; a mounting base is arranged on and connected to the transverse foundation pillars; a plurality of internal heating digestion pipes are vertically arranged in the mounting base in parallel; a lower part of each internal heating digestion pipe is provided with two transverse holes internal heating digestion pipe perpendicular to axes of the internal heating digestion pipes; each transverse hole is internally provided with a through-tube; each through-tube is internally provided with a U-shaped heating wire; each internal heating digestion pipe is corresponding to a switch and a temperature control base; the temperature control bases are fixedly sleeved in the internal heating digestion pipes; the side wall of each temperature control base is equipped with a temperature sensor and two U-shaped heating wires, wherein the temperature sensor and the U-shaped heating wires are connected to the electric appliance control box via conductive wires; and a plurality of switches are installed on the upper end face of the chassis, and are connected to the control box through conductive wires. The digestion device is used for measuring protein in various biological and plant samples.

The invention discloses a milk product true protein determinator and a determination method thereof, and belongs to the technical field of milk product true protein detection. The milk product true protein determinator comprises a potentiometer, a dissolved oxygen electrode, a test cup body, a magnetic stirrer and a current detection device; the bottom of the test cup body is communicated with and fixed on the magnetic stirrer, a stirring rod located on the magnetic stirrer is arranged in an inner cavity of the test cup body, the dissolved oxygen electrode is connected with the potentiometer through a wire, the bottom of the dissolved oxygen electrode stretches into the inner cavity of the test cup body, the current detection device is used for detecting currents of a solution in the inner cavity of the test cup body, and the potentiometer, the dissolved oxygen electrode, magnetic stirrer and the current detection device are controlled by a control system to work. According to the milk product true protein determinator and the determination method thereof, the content of protein in milk products is quickly determined by means of a current method, the content of the protein in the sample is analyzed by determining the current decrease value through a dissolved oxygen meter, and therefore the detection errors caused by existence of non-protein nitrogen are reduced.

The invention discloses a free protein S determination kit and a preparation method thereof. The kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises a first buffer solution, a first stabilizer and a coagulant, and the pH value of the reagent R1 is 6.0-9.0; the R2 reagent comprises anti-human FPS antibody latex particles, a second buffer solution and a second stabilizer, the pH value of the R2 reagent is 6.0-9.0, and FPS is free protein S; the preparation method comprises the following steps: adding a first stabilizer and a coagulant into a first buffer solution, and adjusting the pH value to 6.0-9.0 to prepare a reagent R1; adding anti-human FPS antibody latex particles and a second stabilizer into a second buffer solution, and adjusting the pH value to 6.0-9.0 to prepare a reagent R2; according to the invention, the anti-human FPS antibody is coupled with the latex particles, and the FPS in the human plasma is determined by using the latex enhanced immunoturbidimetry, so that the accuracy is high, the stability is good, and the linear range is wide; the preparation method is simple and stable in process.

The invention discloses a detection system for determining the occupational fatigue degree of key industries based on human saliva protein, and relates to the technical field of fatigue detection. The fatigue detection system comprises a biomarker database, a data acquisition module, a processor module, a cloud database and a mobile terminal, network visual detection is realized by establishing a fatigue crowd biomarker database, a front-end saliva fatigue biomarker parameter acquisition and information processing system is applied to carry out monitoring, the physical quantity data such as the type content of fatigue biomarkers are collected and monitored, the processor module carries out processing and function analysis on the collected data signals, a man-machine interaction interface is established, a fatigue prevention and control technology network system is constructed through cloud data, the data are sent to the monitoring terminal through a network, the fatigue degree of the occupational population is prejudged by prevention and control personnel, prevention and control measures are taken, and a prevention and control system covering links of health management, fatigue detection, risk assessment, alarm intervention and the like is constructed.

The invention designs a series of polypeptide combinational libraries with different lengths. Polypeptides with functions of inhibiting the activity of HCV NS5B RNA polymerase can be screened out by performing molecular docking on the polypeptides and specific inhibitory sites on HCV NS5B RNA polymerase by using molecular docking software and performing comprehensive analysis on results, and can be used for laying a foundation for further researching and developing anti-HCV medicines. Combined experimental results of modified polypeptide and NS5B expression protein show that the designed polypeptides can be combined with expression protein to express that fusion protein measurement results have correlations with virtual docking results. The polypeptides designed by the invention can be used for detecting HCV NS5B protein, and results obtained by performing polypeptide inhibition screening by using an HCV NS5B RNA polymerase in-vitro activity identification system show that the polypeptides designed by the invention have significant inhibition effects on the polymerase activity of HCV NS5B protein, IC50 of the polypeptides can reach 9.85 microns, and the polypeptides can be used as potential medicines for inhibiting HCV.

Provided is a glycated protein assay reagent containing at least a Trinder reagent, 4-aminoantipyrine, protease, a stabilizer of the protease, and ferrocyanide, wherein at least the Trinder reagent is contained in a Trinder reagent-containing partial composition, at least the 4-aminoantipyrine is contained in a 4-aminoantipyrine-containing partial composition, the stabilizer of the protease is a stabilizer that increases the oxidation-reduction potential of the ferrocyanide above 0.058 V when the stabilizer of the protease and the ferrocyanide are mixed, and the oxidation-reduction potential is an oxidation-reduction potential in a reaction system containing the stabilizer of the protease and the ferrocyanide and not containing glycated protein.