This invention discloses a cerebrospinal fluid or urinetotal protein detection kit, belonging to the technical field of detection kits. The kit includes reagent R1 and reagent R2, wherein reagent R1 includes a buffer stabilizer Tris to maintain the stability of reagent R1 during the opening process. This invention solves the problems of easy pH drop and poor stability of existing turbidimetric total proteinassay kits after opening. The cerebrospinal fluid or urinetotal protein detection kit, by adding Tris to reagent R1, can maintain the stability of small amounts of reagent after opening, which is beneficial for further promotion and use in the market, ensuring the long-term reliability of test results and the economy of reagent use.