The invention discloses a preparation method and application of a bioactive nanometer organic zinc and selenium animal and plant nutrient solution. The method comprises the following steps: weighing raw materials in the following mass percent, and adding the following raw materials in every 5000kg of water: 8 to 12kg of maltose, 15 to 25kg of sodium cyclamate, 15 to 25g of gelatin, 15 to 25g of chitosan, 25 to 35g of zinc oxide, 450 to 550g of lysine, 35 to 45g of sodium tetraborate, 95 to 105g of glycine betaine, 95 to 105g of nonprotein nitrogen, 95 to 105g of beta-glucolase, 450 to 550g of saccharomycetes, 450 to 550g of selenium philic bacillus laterosporus, and 25 to 35g of selenium dioxide; weighing and uniformly mixing and stirring the raw materials, and performing fermentation on the raw materials for 15 to 20 days at 25 to 35 DEG C, so as to obtain the nutrient solution. The nutrient solution can not only ensure that plants accelerate absorb and enrich organic zinc and selenium, but also can promote the plants to perform in vivo absorption on various nutrients beneficial to growth and improvement of the mouthfeel, enables the egg dead rate to be greater than 95 percent, is odorless, environmental-friendly, convenient to apply, uniform, does not destroy roots and seedlings, improves the plant quality and yield, and can further improve the antiviral and anti-infective ability of animals.